Nuclear magnetic resonance-based metabolomics and metabolic pathway networks from patient-matched esophageal carcinoma,adjacent noncancerous tissues and urine

BACKGROUND Several studies have demonstrated a correlation between esophageal cancer(EC)and perturbed urinary metabolomic profiles,but none has described the correlation between urine metabolite profiles and those of the tumor and adjacent esophageal mucosa in the same patient.AIM To investigate how urinary metabolic phenotypes were linked to the changes in the biochemical landscape of esophageal tumors.METHODS Nuclear magnetic resonance-based metabolomics were applied to esophageal tumor tissues and adjacent normal mucosal tissues alongside patient-matched urine samples.RESULTS Analysis revealed that specific metabolite changes overlapped across both metrics,including glucose,glutamate,citrate,glycine,creatinine and taurine,indicating that the networks for metabolic pathway perturbations in EC,potentially involved in but not limited to disruption of fatty acid metabolism,glucose and glycolytic metabolism,tricarboxylic acid cycle and glutaminolysis.Additionally,changes in most urinary biomarkers correlated with changes in biomarker candidates in EC tissues,implying enhanced energy production for rapid cell proliferation.CONCLUSION Overall,these associations provide evidence for distinct metabolic signatures and pathway disturbances between the tumor tissues and urine of EC patients,and changes in urinary metabolic signature could reflect reprogramming of the aforementioned metabolic pathways in EC tissues.Further investigation is needed to validate these initial findings using larger samples and to establish the underlying mechanism of EC progression.

Exposure to Hyaluronan and Radon-Containing Water during the Treatment of Periodontal Pockets

Hyaluronic acid (HA) preparations have emerged as pivotal components in contemporary dentistry, gaining widespread recognition for their multifaceted roles in various biological functions. Extensive literature underscores the significance of HA in maintaining tissue water balance, fostering cell proliferation, promoting rapid cell migration, influencing cell differentiation during organism development, and facilitating tissue regeneration. Notably, HA’s interactions with cell surface receptors contribute to the viscosity of synovial fluid, activate the immune system, and enhance cartilage elasticity. Beyond these established functions, HA has also been investigated for its potential involvement in determining and studying the hormetic effects of radon water, adding a novel dimension to its applications in dental research. A thorough exploration of existing studies reveals a nuanced understanding of how HA interventions impact the outcomes of dental procedures. The comprehensive scope of these investigations allows for a more accurate assessment of the potential effectiveness of specific interventions and provides valuable insights into post-procedural prognoses for individual patients. This synthesis of literature serves as the foundation for elucidating the intricate interplay between HA, radon exposure, and their relevance in modern dental practices.

Mesenchymal stromal cells from human perinatal tissues: From biology to cell therapy

Cell-based regenerative medicine is of growing interest in biomedical research. The role of stem cells in this context is under intense scrutiny and may help to define principles of organ regeneration and develop innovative therapeutics for organ failure. Utilizing stem and progenitor cells for organ replacement has been conducted for many years when performing hematopoietic stem cell transplantation. Since the first successful transplantation of umbilical cord blood to treat hematological malignancies, non-hematopoietic stem and progenitor cell populations have recently been identified within umbilical cord blood and other perinatal and fetal tissues. A cell population entitled mesenchymal stromal cells (MSCs) emerged as one of the most intensely studied as it subsumes a variety of capacities: MSCs can differentiate into various subtypes of the mesodermal lineage, they secrete a large array of trophic factors suitable of recruiting endogenous repair processes and they are immunomodulatory.Focusing on perinatal tissues to isolate MSCs, we will discuss some of the challenges associated with these cell types concentrating on concepts of isolation and expansion, the comparison with cells derived from other tissue sources, regarding phenotype and differentiation capacity and finally their therapeutic potential.

Gene editing for corneal disease management

Gene editing has recently emerged as a promising technology to engineer genetic modifications precisely in the genome to achieve long-term relief from corneal disorders.Recent advances in the molecular biology leading to the development of clustered regularly interspaced short palindromic repeats(CRISPRs) and CRISPR-associated systems,zinc finger nucleases and transcription activator like effector nucleases have ushered in a new era for high throughput in vitro and in vivo genome engineering.Genome editing can be successfully used to decipher complex molecular mechanisms underlying disease pathophysiology,develop innovative next generation gene therapy,stem cell-based regenerative therapy,and personalized medicine for corneal and other ocular diseases.In this review we describe latest developments in the field of genome editing,current challenges,and future prospects for the development of personalized genebased medicine for corneal diseases.The gene editing approach is expected to revolutionize current diagnostic and treatment practices for curing blindness.

Cemented vertebra and adjacent vertebra refractured in a chronic kidney disease-mineral and bone disorder patient: A case report

BACKGROUND Although percutaneous vertebral augmentation(PVA)is a commonly used procedure for treating vertebral compression fracture(VCF),the risk of vertebral refracture should be considered.Chronic kidney disease-mineral and bone disorder(CKD-MBD)is a systemic disease of mineral and bone metabolism.It is associated with an increased risk of fracture.Few studies have reported the use of PVA in patients with CKD-MBD.We herein report a rare case wherein the cemented vertebra and the adjacent vertebra refractured simultaneously in a CKD-MBD patient after PVA.CASE SUMMARY A 74-year-old man suffered from low back pain after taking a fall about 3 wk ago.According to physical examination,imaging and laboratory findings,diagnoses of T12 VCF,CKD-MBD,and chronic kidney disease stage 5 were established.He then received percutaneous vertebroplasty at T12 vertebra.Fourteen weeks later,he presented with T12 and L1 vertebral refractures caused by lumbar sprain.Once again,he was given PVA which was optimized for the refractured vertebrae.Although the short-term postoperative effect was satisfactory,he reported chronic low back pain again at the 3-month follow-up.CONCLUSION It is necessary that patients with CKD-MBD who have received PVA are aware of the adverse effects of CKD-MBD.It may increase the risk of vertebral refracture.Furthermore,the PVA surgical technique needs to be optimized according to the condition of the patient.The medium-and long-term effects of PVA remain uncertain in patients with CKD-MBD.

Similarities and differences between mesenchymal stem/progenitor cells derived from various human tissues

BACKGROUND Mesenchymal stromal/stem cells (MSCs) constitute a promising tool in regenerative medicine and can be isolated from different human tissues. However, their biological properties are still not fully characterized. Whereas MSCs from different tissue exhibit many common characteristics, their biological activity and some markers are different and depend on their tissue of origin. Understanding the factors that underlie MSC biology should constitute important points for consideration for researchers interested in clinical MSC application. AIM To characterize the biological activity of MSCs during longterm culture isolated from: bone marrow (BM-MSCs), adipose tissue (AT-MSCs), skeletal muscles (SMMSCs), and skin (SK-MSCs). METHODS MSCs were isolated from the tissues, cultured for 10 passages, and assessed for: phenotype with immunofluorescence and flow cytometry, multipotency with differentiation capacity for osteo-, chondro-, and adipogenesis, stemness markers with qPCR for mRNA for Sox2 and Oct4, and genetic stability for p53 and c-Myc;27 bioactive factors were screened using the multiplex ELISA array, and spontaneous fusion involving a co-culture of SM-MSCs with BM-MSCs or AT-MSCs stained with PKH26 (red) or PKH67 (green) was performed. RESULTS All MSCs showed the basic MSC phenotype;however, their expression decreased during the follow-up period, as confirmed by fluorescence intensity. The examined MSCs express CD146 marker associated with proangiogenic properties;however their expression decreased in AT-MSCs and SM-MSCs, but was maintained in BM-MSCs. In contrast, in SK-MSCs CD146 expression increased in late passages. All MSCs, except BM-MSCs, expressed PW1, a marker associated with differentiation capacity and apoptosis. BM-MSCs and AT-MSCs expressed stemness markers Sox2 and Oct4 in long-term culture. All MSCs showed a stable p53 and c-Myc expression. BM-MSCs and AT-MSCs maintained their differentiation capacity during the follow-up period. In contrast, SK-MSCs and SM-MSCs had a limited ability to differentiate into adipocytes. BM-MSCs and AT-MSCs revealed similarities in phenotype maintenance, capacity for multilineage differentiation, and secretion of bioactive factors. Because AT-MSCs fused with SM-MSCs as effectively as BM-MSCs, AT-MSCs may constitute an alternative source for BM-MSCs. CONCLUSION Long-term culture affects the biological activity of MSCs obtained from various tissues. The source of MSCs and number of passages are important considerations in regenerative medicine.

玻璃化法保存异体动脉的实验研究

目的 探讨玻璃化法保存动脉的可行性和异体移植的有效性。 方法 家兔6 0只。其中2 4只切取双侧股动脉共计4 8条,平分为2组,分别采用玻璃化法和低温冷冻法保存14 d,作为移植材料。其余36只作为移植对象,平分为3组,每组12只2 4条动脉。A组行新鲜家兔动脉自体移植,B组行玻璃化保存家兔动脉异体移植,C组行低温冷冻保存家兔动脉异体移植。移植前对动脉进行大体和组织学观察。术后行动脉造影了解移植后动脉的通畅率,并分别于术后14、30、6 0和12 0 d取材,对动脉进行大体和组织学观察,测定内膜/中膜比值,并进行组间比较。 结果 移植前B组的动脉完整率为91.6 7% ,优于C组的5 4 .17% ,二者差异有统计学意义(P<0 .0 1)。移植后B组的累计通畅率达到87.5 0 % ,与A组差异无统计学意义,但优于C组的6 6 .6 7% ,二者差异有统计学意义(P<0 .0 5 )。B组的内膜/中膜比值与A、C组比较,差异有统计学意义(P<0 .0 5 )。 结论 玻璃化法保存动脉在降温和复温过程中冰晶较少,对动脉整体结构和活性影响较小,能较好地保存动脉的组织结构;玻璃化法保存的动脉异体移植的效果优于低温冷冻法保存的动脉异体移植。

虎耳草属两种虎耳草营养器官的组织解剖学鉴定

对虎耳草及三色虎耳草的形态特征及显微结构等方面进行了比较研究.结果表明:虎耳草的叶较小,近圆形,叶片宽阔肥厚,边缘圆齿状,深绿色,网状叶脉处有白色条纹,表皮细胞较小,表皮绒毛较多且长,叶柄细且长,葡匐茎,紫红色,颜色较浅,叶柄及叶内都有蔟晶分布,茎和叶柄内有较多淀粉,根内初生木质部呈六棱形.而三色虎耳草的叶较大,肾形,叶片较薄,近边缘处具乳白粉红斑宽边,翠绿色,背面有紫红色斑点,表皮细胞较大,表皮绒毛较少且短,叶柄粗且短,匍匐茎,紫红色,颜色较深,叶柄及叶内也有蔟晶分布,茎和叶柄内有很多淀粉粒,根内初生木质部呈三棱形.这些特征的比较为药材的开发和利用提供了科学依据.

不同剂量甜菜碱对热应激肉鸡组织器官发育的影响

选择将1日龄肉用仔鸡200只,随机分成3组,在试验期内,环境温度为28～31℃,湿度为83～87%。对照组仅喂基础日粮,试验1组、试验2组分别在基础日粮添中加0.1%、0.3%的甜菜碱。结果表明:试验1组鸡在38～47日龄甲状腺的重量和肾上腺重量显著增加(P<0.05),在48～57日龄试验1组鸡胸腺、胃、肝脏、心脏的重量显著增加(P<0.05),睾丸的重量极显著增加。在试验期内,与对照组相比较,试验1组鸡胰、脾、法氏囊、肺和肾重量差异都不显著(P>0.05)。试验2组鸡除了在48～57日龄睾丸重量极显著增加(P<0.01)以外,其它器官重量差异均不显著(P>0.05)。试验2组与试验1组鸡在整个试验期内各器官重量差异均不显著(P>0.05)。组织学观察表明,在高温环境中,对照组鸡肾、心脏、肺、肝、胃、脾、胸腺等器官出现不同程度病理变化,但试验1组鸡上述组织器官未见有明显变化,这说明,甜菜碱具有一定的抗热应激作用。

转基因741杨高效配套育苗体系建立

针对转基因741杨硬枝扦插难生根的特性,将组织培养及多种扦插繁殖技术有机结合在一起,建立了转基因741杨综合配套育苗体系,提出了适合无性繁殖的"遮阴间歇喷雾扦插"和"移棚不移苗扦插繁殖"育苗方法。转基因741杨组培快繁中,初始启动培养基为MS+6-BA1.0 mg/L+NAA0.1 mg/L,嫩茎增殖培养基为MS +6-BA 0.3-1.0 mg/L+,IBA 0-0.3 mg/L,茎尖生根培养基为1/2MS+0.3IBA+1.5%蔗糖。不同月份嫩枝扦插培养的苗木中,5月份扦插的嫩枝生长较快,但苗木间分化程度较高;8月份扦插的嫩枝生长相对较慢,但苗木的分化程度低,生长整齐。不同月份苗木移栽后,生长节律相同,经过1 a生长,不同月份间苗木生长差异不明显,5月份扦插的苗木生长略优于其他月份扦插的苗木。

Analyzing proteins in colonic tissues from mice with ulcerative colitis using the iTRAQ technology

Objective The aim of the study was to investigate the expression of proteins in colonic tissues of mice with ulcerative colitis(UC) by using isobaric tags for relative and absolute quantitation(iTRAQ), probe into the pathogenesis of UC, and find potential biomarkers of UC. Methods Forty C57 mice were randomly divided into the control and model groups(20 mice in each group). The mice in the model group were administered dextran sulphate sodium(DSS) for 7 consecutive days ad libitum to induce acute colitis, and the colon tissue was extracted on the 8 th day after the successful establishment of the UC model. Proteins were identified by the i TRAQ and tandem mass spectrometry techniques,and the identified proteins were analyzed by bioinformatics. Results A total of 4019 proteins were identified among the two groups. Among them, 317 significant differentially expressed proteins(DEPs) were detected according to the screening criteria for selecting DEPs, i.e. fold change ratios ≥ 1.5 or ≤ 0.67 and P-values < 0.05, of which 156 were upregulated and 161 were downregulated. In the Gene Ontology(GO) analysis, the DEPs were classified into 48 functional categories, which contained biological process, cellular component, and molecular function. Based on the 317 DEPs, the KEGG pathway analysis identified 160 vital pathways.Conclusion DEPs in colonic tissues of mice with UC were screened using the iTRAQ technique, which laid a foundation for further studies regarding the pathogenesis of UC.

复合胶原的组织工程肌腱力学性能的实验研究

目的 探讨胶原在组织工程肌腱的构建中对力学强度的影响。 方法 裸鼠75只,制成跟腱缺损的动物模型,随机分为5组,分别将人发(hair,H)、碳纤维(carbon fiber,CF)、聚羟基乙酸(polyglycolic acid,PGA)、H +PGA和CF+PGA复合外源性 型胶原,与标准转化人胚腱细胞株联合培养制成组织工程肌腱。植入裸鼠右后肢修复跟腱缺损,为实验组(A组) ;将未复合胶原的上述各种材料植入裸鼠左后肢修复跟腱缺损,为对照组(B组)。术后于2、4、6、8和12周进行生物力学测定,观察并比较其变化。 结果 实验组2～4周力学强度高于对照组,差异有统计学意义(P<0 .0 5 ) ,但6～12周两组差异无统计学意义(P>0 .0 5 ) ;时间与力学强度呈正相关,差异有统计学意义(P<0 .0 5 )。其中以人发的力学强度最佳,CF次之,PGA最差。 结论 外源性胶原在肌腱修复的早期能增强组织工程肌腱的力学强度,对患肢功能的恢复有一定的作用。

断奶前后仔猪胃组织中ghrelin基因表达及内源性生长抑素的作用

目的:研究仔猪断奶前后胃组织中ghrelin基因表达和其对胃组织发育性变化的影响,同时对内源性生长抑素的作用也进行了研究. 方法:实验选取新生仔猪18窝,随机分为实验组和对照组,自12 d起,对照组饲喂基础乳猪料,实验组在基础日粮中添加120 mg半胱胺,以耗竭内源性生长抑素,两组仔猪均于35 d断奶.分别于28,35,36.5,38, 42和45 d随机选取实验组和对照组仔猪各6头,屠宰, 称量胃重和体重并采集胃底和胃窦黏膜,用RT—PCR 方法,以18S rRNA作内标,定量分析胃底和胃窦组织中ghrelin mRNA的丰度. 结果:胃底ghrelin mRNA的丰度明显高于胃窦部;胃底ghrelin mRNA水平在35和36.5 d较低,随后逐渐增加.45 d ghrelin mRNA水平显著高于35,36.5和38 d (P<0.05);半胱胺能提高胃底ghrelin mRNA表达,其中35,36.5和38 d时试验组ghrelin mRNA表达显著高于对照组(P<0.05). 结论:Ghrelin能直接或间接作用于胃组织,调节胃的生长,表明Ghrelin与胃的形态和功能发育具有密切的关系;内源性生长抑素可抑制胃底ghrelin mRNA的表达.

米良一号猕猴桃的组织培养研究

米良一号猕猴桃外植体在不同激素的MS培养基上培养,结果表明:1诱导培养基以MS+1.0mg/LZT为好,材料不经转移可直接分化出丛生芽,ZT浓度在1.5mg/L以上时,对愈伤组织形成有抑制作用,且在1.5mg/LZT的同时,加0.5mg/L6-AB,这2种细胞分裂素超量后的抑制作用是累加的,致使抑制作用加剧无芽茎段褐化死亡;2出芽周期长短与茎段年龄和有芽无芽有关,成熟茎段出芽早,幼嫩茎段出芽迟,有芽茎段出芽早,无芽茎段出芽迟,幼嫩的无芽茎段出芽虽晚30d左右,但由愈伤组织形成的丛芽多得多;3米良一号生根能力不强,出芽后不经生根诱导不能生根,1/2MS+1.0mg/LIBA+30g/L蔗糖,有较好的诱导生根作用;4叶片培养再生率正交实验结果表明生长素优先因子顺序为ZT、6-BA、NAA、TDZ,叶的最佳诱导分化培养基为MS+10mg/LBA+0.2mg/LNAA+1.0mg/LTDZ。

仿生双相磷酸钙生物陶瓷支架的Micro-CT评价

[目的]体外用MicroCT(Microcomputedtomography)图像对仿生双相磷酸钙生物陶瓷支架的三维结构进行计算机重建和评价。[方法]犬股骨头的松质骨样本行MicroCT扫描,提取图像信息;三维凝胶叠层成型法制备出具有仿骨小梁结构的双相磷酸钙仿生生物陶瓷支架。随后用MicroCT对支架扫描,以三维结构参数对支架和松质骨样本作三维评价和比较。三维参数包括骨体积分数(BoneVolumeFraction,BVF,BV/TV)、骨表面积体积比(Bonesurface/bonevolumeratio,BS/BV)、骨小梁厚度(Trabecularthickness,TbTh)、骨小梁数目(Trabecularnumber,TbN)、骨小梁间隙(Trabecularspacing,TbSp)、结构模型指数(StructureModelIndex,SMl)和各向异性程度(degreeofanisoropy,DA)。[结果]与犬股骨头松质骨样本相比,用本方法制备出的BCP支架具有相似的三维空间结构,二者的BV/TV、TbTh、TbN无显著差别(P>0.05)。小梁结构呈板状模型。[结论]本研究制备的BCP多孔支架的小梁具有一定的取向性,力学强度和良好的适于血管长入的空间结构。

壳寡糖对肉仔鸡生产性能、小肠组织结构和肌组织矿物质元素含量的影响

采用单因子试验设计,选1日龄28000只AA肉仔鸡,随机分为4组,以研究饲料中添加不同剂量(0g/t、5g/t、25g/t、125g/t)的壳寡糖对生产性能、消化器官生长发育、小肠组织结构以及胸、腿肌组织中矿物元素含量的影响。研究结果表明:随壳寡糖添加剂量增加各周龄平均体重增加, 7周龄出栏时壳寡糖组(125g/t)平均体重增加140g,提高5. 85%,料重比降低3. 93%;与对照组比较壳寡糖组(125g/t)肉仔鸡的肝脏、胆囊、胰腺、嗉囊、肌胃和腺胃的相对重量均降低,胰腺和腺胃分别降低7. 19%和10. 34%,差异显著(p<0. 05);十二指肠、空肠、回肠、盲肠和结直肠相对重量均减小,十二指肠减少14. 05%、盲肠和结直肠分别减少12. 75%和19. 59%,有显著差异(p<0. 05);空肠和回肠的绒毛高度(p<0. 05)和十二指肠、空肠、回肠的隐窝深均增加(p<0. 05),而绒毛宽度减小(p<0. 05),肠壁和粘膜厚度下降(p<0. 05);壳寡糖组(125g/t)肉仔鸡胸肌中除P元素减少外, Ca、Zn、Fe和Mn元素含量均比对照组高(p<0. 05);腿肌中Ca、P、Zn、Fe和Mn元素含量均比对照组高,除Mn外各元素差异显著(p<0. 05)。说明壳寡糖对肉仔鸡具有降低肠壁厚度、改善肠粘膜结构、提高消化器官功能,从而利于营养物质的吸收,促进生长的作用。同时,还能增加胸、腿肌中Ca、P、Zn。

术中组织印片法快速诊断212例分析

目的探讨组织印片在术中快速诊断的可行性。方法将术中送检的212例新鲜标本,即刻切开,在不同病变切面上用载玻片轻轻触印,制成印片,快速HE染色,光镜下观察,报告诊断结果。所剩组织均做石蜡切片诊断作对照。结果212例印片诊断与石蜡切片诊断比较,印片阳性(恶性肿瘤)57例(26.9%);阴性(非肿瘤性)146例(68.9%),印片诊断准确率95.8%(203/212)。石蜡切片诊断阳性64例(30.2%),阴性148例(69.8%),准确率100.0%(212/212)。用印片诊断阳性57例与石蜡切片诊断作类型比较,有38例完全相符,诊断符合率66.7%(38/57)。7例误诊,误诊率仅为3.3%(7/212)。3例定性准确但分类不符合,9例病变交界难定。结论组织印片诊断不同于一般涂片单纯细胞学诊断,而是细胞学与组织学相互结合观察的结果,有时可进行组织类型的诊断。在冷冻切片与快速石蜡切片同时作快速印片,可有效纠正部分误诊。

MicroRNAs as potential biomarkers for diagnosis of schizophrenia and influence of antipsychotic treatment

Chara cterized by positive symptoms(such as changes in behavior or thoughts,including delusions and hallu cinations),negative symptoms(such as apathy,anhedonia,and social withdrawal),and cognitive impairments,schizophrenia is a chro nic,severe,and disabling mental disorder with late adolescence or early adulthood onset,Antipsychotics are the most commonly used drugs to treat schizophrenia,but those currently in use do not fully reverse all three types of symptoms characte rizing this condition.Schizophrenia is frequently misdiagnosed,resulting in a delay of or inappropriate treatment.Abnormal expression of microRNAs is connected to brain development and disease and could provide novel biomarkers for the diagnosis and prognosis of schizophrenia.The recent studies reviewed included microRNA profiling in blood-and urine-based materials and nervous tissue mate rials.From the studies that had validated the preliminary findings,potential candidate biomarkers for schizophrenia in adults could be miR-22-3p,-30e-5p,-92a-3p,-148b-5p,-181a-3p,-181a-5p,-181b-5p,-199 b-5p,-137 in whole blood,and miR-130b,-193a-3p in blood plasma.Antipsychotic treatment of schizophrenia patients was found to modulate the expression of certain microRNAs including miR-130b,-193a-3p,-132,-195,-30e,-432 in blood plasma.Further studies are warranted with adolescents and young adults having schizophrenia and consideration should be given to using animal models of the disorder to investigate the effect of suppressing or overexpressing specific microRNAs.

Parameters Optimization and Performance Evaluation of the Tuned Inerter Damper for the Seismic Protection of Adjacent Building Structures

In order to improve the seismic performance of adjacent buildings,two types of tuned inerter damper(TID)damping systems for adjacent buildings are proposed,which are composed of springs,inerter devices and dampers in serial or in parallel.The dynamic equations of TID adjacent building damping systems were derived,and the H2 norm criterion was used to optimize and adjust them,so that the system had the optimum damping performance under white noise random excitation.Taking TID frequency ratio and damping ratio as optimization parameters,the optimum analytical solutions of the displacement frequency response of the undamped structure under white noise excitation were obtained.The results showed that compared with the classic TMD,TID could obtain a better damping effect in the adjacent buildings.Comparing the TIDs composed of serial or parallel,it was found that the parallel TIDs had more significant advantages in controlling the peak displacement frequency response,while the H2 norm of the displacement frequency response of the damping system under the coupling of serial TID was smaller.Taking the adjacent building composed of two ten-story frame structures as an example,the displacement and energy collection time history analysis of the adjacent building coupled with the optimum design parameter TIDs were carried out.It was found that TID had a better damping effect in the full-time range compared with the classic TMD.This paper also studied the potential power of TID in adjacent buildings,which can be converted into available power resources during earthquakes.

Clinical efficacy,bone density,and follow-up in implant and orthodontic treatment for inclined adjacent teeth

BACKGROUND Tooth defects can cause elongation of occlusal teeth,leading to insufficient repair space.The combination of dental implant restoration and orthodontic treatment of oblique adjacent teeth has a significant therapeutic effect.AIM To explore clinical efficacy,bone density,and follow-up of implant and orthodontic treatment for patients with inclined adjacent teeth.METHODS In total,98 patients with oblique adjacent teeth were randomly assigned to implant restoration combined with orthodontic treatment(group A,n=49)or to receive implant restoration alone(group B,n=49).Changes in alveolar ridge bone density and apical bone density were observed before and after treatment in the two groups.Changes in chewing function and language function were compared between the two groups of patients.Follow-up lasted for 12 mo after repair to observe any adverse reactions in the oral cavity.RESULTS The clinical effective rates of group A and group B were 97.96%and 85.71%,respectively,with group A having a higher clinical effective rate than group B.After treatment,the bone density of the alveolar ridge and apical bone in both groups decreased compared to before treatment,while the chewing and language functions improved.The changes in various indicators in group A were more significant.After treatment,the satisfaction rate of group A(97.96%)was higher than that of group B(79.59%).The incidence of adverse reactions in group A(2.04%)was lower than that in group B(24.49%).CONCLUSION The amalgamation of implant restoration and orthodontic treatment for adjacent tilted teeth demonstrates notable clinical efficacy,diminishes alveolar bone resorption,and fosters patient functional rehabilitation while exhibiting negligible adverse reactions.