How to promote cooperative innovation in environmentally friendly technology:a case of agricultural biotechnology in China

This study explored the factors influencing cooperative innovation in environmentally friendly agricultural biotechnology in China.First,we constructed an evolutionary game model comprising the factors of net income of cooperative innovation,net income of independent innovation,market constraints,and government subsidies.Using MATLAB simulation,we assigned different values to the aforementioned variables to explore the evolutionary trend of innovators’willingness to cooperate.Results showed that when the values of net income of cooperative innovation,net income of independent innovation,market constraints,and government subsidies exceeded the threshold,innovators’willingness to cooperate was significantly enhanced.Furthermore,the proportion of innovators who cooperated with others gradually increased to 100%;otherwise,it gradually decreased to 0%.Comparing the simulation curve with the real evolution curve of cooperative innovation in agricultural biotechnology in China,we found that the gradual decline in the willingness to cooperate could be due to insufficient subsidies for cooperative innovation,low income from cooperative innovation,weak profitability of innovators,and weak market constraints.

Cytotoxicity Tests for Evaluating Medical Devices: An Alert for the Development of Biotechnology Health Products

The risks and damages related to the use of products, technologies and services of sanitary interest can be due to defects or manufacturing failures. Certain products already contain a certain degree of risk, which requires strict quality control in their production, distribution and use, as well as in the disposal of their waste in the environment. With continuous development in science and technology, medical devices must undergo intradermal irritation and testing for sensitization, cytotoxicity, and acute systemic toxicity. In health care, biotechnology aims to provide technology-based products or processes related to energy, food, and health, which are capable of stimulating new businesses, expanding exports, integrating the value chain and stimulating new demands for innovative products and processes, taking into account health policies. The present article was prepared by a bibliographical survey of the electronic databases PubMed, Lilacs, and Bireme. Cell culture testing can be successfully employed, as it is reproducible, rapid, sensitive, and financially accessible for performing in vitro toxicity testing. Thus, it has been possible to optimize the development phase of new products by decreasing animal use or even replacing them in certain tests. Some in vitro assays validated by the Organization for Economic Cooperation and Development in the area of health products have already replaced animal testing.

Single-molecule biotechnology for protein researches

Cells employ proteins to perform metabolic functions and maintain active physiological state through charge transfer and energy conversion.These processes are carried out in a narrow space precisely and rapidly,which,no doubt,bring great difficulty for their detection and dissection.Fortunately,in recent years,the development and expansion of single-molecule technique in protein research make monitoring the dynamical changes of protein at single-molecule level a reality,which also provides a powerful tool for the further exploration of new phenomena and new mechanisms of life activities.This paper aims to summarize the working principle and essential achievements of single-molecule technique in protein research in recent five years.We focus on not only dissecting the difference of nanopores,atomic force microscope,scanning tunneling microscope,and optical tweezers technique,but also discussing the great significance of these single-molecule techniques in investigating intramolecular and intermolecular interactions,electron transport,and conformational changes.Finally,the opportunities and challenges of the single-molecule technique in protein research are discussed,which provide a new door for single-molecule protein research.

“Essentials of Biotechnology”来华留学英语授课品牌课程建设纪实

经过十多年的建设,"Essentials of Biotechnology"已建设成为江南大学乃至江苏省来华留学生英语授课品牌课程。该课程针对来华留学生学科背景有较大差异,英语能力参差不齐和课程知识涉及面广,知识点较多,理论性强等问题,在原有双语课程教学的基础上,通过优化教材和教学内容,打造双向教学模式和建设多元评价体系并持续改进,构建了多层次启发式、互动、研讨相结合的复合教学体系,显著增强留学生专业基础知识水平和自主学习能力,为后续课程学习和研究工作的开展奠定了良好的基础。教学实践结果表明,该教学体系对提高该类课程的学习成效和课程质量,具有较为显著的示范效应。

Application of Biotechnology on Apple Breeding

Modern biotechnology is a kind of potential technology. The advantages of this method have aroused much attention. Recently, biotechnology has evolved very fast in apple tree, and has got much progress in production and breeding of seedlings. This paper summarized the advances in tissue culture, molecular marker and gene engineering. The emphases and direction which need further investigation were also discussed.

Reform of Teaching Practice of Modern Instrumental Analysis in Biotechnology Programs

Through the online and offline practical reform of the Modern Instrumental Analysis course of biotechnology majors from teaching content,teaching method,teaching demonstration to teaching effect,the traditional single offline lecture is transformed into diversified and interactive modern teaching.The practical reform enriches and optimizes the course content,perfects and improves the course assessment system,and improves the teaching quality.It achieves the student-centered and application-oriented teaching goal,and also provides reference for further cultivating high-quality applied talents.

Tapping Wet Potential China and Malaysia join hands to develop marine biotechnology

Kappaphycus alvarezii(K.alvarezii)is a perennial large algae native to tropical and subtropical regions.With a slender thallus,it branches better at the upper part than the lower end.The sea moss is mainly found in Hainan Province,China,and Southeast Asian countries such as Malaysia and the Philippines because it grows best in the subtidal zone of clean.

Success Factors of Biotechnology Industry Based on Triangular Fuzzy Number

Based on the theory of competitive advantage and value chain, this paper establishes the indicator system, and develop the strategic framework using the fuzzy Delphi method. Then the triangular fuzzy number model is established using Fuzzy Analytic Hierarchy Process, and the key factors influencing biotechnology industry are extracted. The results show that in terms of weight, the key factors influencing the success of biotechnology industry are sequenced as follows: "open innovation capacity", "quality and cost control ability", "advanced customer-oriented product manufacturing capacity", "technology R & D personnel's capacity", "brand image building capacity", "logistics and sales capacity", "grasping the market demand trends". The manufacturers and government decision-making body can use this as the basis, to promote the development of the biotechnology industry.

Construction and Exploration of Teaching Reform about the Horticultural Plant Biotechnology Course

Horticultural Plant Biotechnology is a core course of undergraduate teaching of horticulture with high practicality.For students,its contents are too complex and abstract to master.In order to strengthen the teaching effect of the course,stimulate students’interest in learning,improve students’ability to analyze and solve problems,this article makes an in-depth analysis of the complicated and abstract knowledge points,the endless emergence of new knowledge,the disconnection between practice and experimental teaching in the Horticultural Plant Biotechnology course.Based on the analysis,it puts forward the reasonable and blend methods of online teaching,to cultivate innovative and practical ability of students.

Advanced Biotechnology for Cell Cryopreservation

Cell cryopreservation has evolved as an important technology required for supporting various cell-based applications,such as stem cell therapy,tissue engineering,and assisted reproduction.Recent times have witnessed an increase in the clinical demand of these applications,requiring urgent improvements in cell cryopreservation.However,cryopreservation technology suff ers from the issues of low cryopreservation effi ciency and cryoprotectant(CPA)toxicity.Application of advanced biotechnology tools can signifi cantly improve post-thaw cell survival and reduce or even eliminate the use of organic solvent CPAs,thus promoting the development of cryopreservation.Herein,based on the diff erent cryopreservation mechanisms available,we provide an overview of the applications and achievements of various biotechnology tools used in cell cryopreservation,including trehalose delivery,hydrogel-based cell encapsulation technique,droplet-based cell printing,and nanowarming,and also discuss the associated challenges and perspectives for future development.

SEAWEED CULTIVATION: A NEW APPLIED FIELD FOR BIOTECHNOLOGY

Seaweeds cultivation has resulted in great achievements, although it has a history ofonly a few decades. With higher productivity and resulting higher profit, it has become the leading marineexploitation industry with the brightest prospects. The relatively limited species introduced for commercialcultivation showed great biological diversity. Introduction of selected good strains for tradtional cultivationand the transformation from cultivation of mixed stains to that of pure cell lines are two certain tendenciesin the future. Pure line cultivation of seaweeds is a sort of advanced biotechnology. It provides new op-portunities for not only the industry itself, but also the stable market of high quality natural marine prod-ucts. More work should be done on principles and methods to obtain optimal results for the combinationof pure line cultivation techniques with advanced biochemistry. The Programmed batch production of finechemicals such as polysaccharides and proteins will probably become the social demand.

For a Holistic View of Biotechnology in West and Central Africa: What Can Integrated Development Approaches Contribute?

Africa, ever on the lookout for development levers that will allow its economy to take off, is turning more and more towards technology. This is one of the possible modern avenues to success, especially the use of the biotechnologies that are so touted by Western countries. However, the hope placed in these new technologies must not hide the long-proven fact that technology alone is not enough to solve development problems. Biotechnologies do not escape this rule. Biotechnologies can be the best and the worst things for the people of Africa. Beyond their technical contributions, we must be wary of their boomerang effects and collateral damage. A country’s development is actually more complex than simply implementing technology, and in the current global environmental context a holistic vision is necessary to ensure sustainable development. In the area of water, this integrated vision emerged on the international scene during the Dublin Conference in 1992, which consecrated the principles of Integrated Water Resources Management (IWRM). More recently, the Eco-Health concept strives to combine human health and ecosystem health while incorporating a socioeconomic dimension into the health and environmental spheres. The concern to mesh human activities better with environmental protection was materialized previously, in the 1970s already, through impact studies. After presenting this set of tools in the service of a holistic approach to the environment and development, we shall see that these approaches can inspire the players when it comes to the ways they implement biotechnologies. At the end of the day, a holistic approach to biotechnologies in Africa will be facilitated by enhanced information and communication and reliance on peasant farmers’ expertise. It will have to be rooted in broader participation of the players concerned. This integration will also concern environmental and land-owning aspects, without forgetting socio-cultural acceptance of the projects and the links with health. Ultimately, it will also mean putting the human at the heart of development by taking all the richness and particularities of African society into account.

Translating laboratory anti-aging biotechnology into applied clinical practice:Problems and obstacles

Although the use of biomedical technologies against ageing(rejuvenation biotechnologies) is considered by many as an effective way of controlling all age-related degeneration,in reality this belief cannot be justified. The human body is notoriously resistant to external perturbations and can respond in unpredictable or undesirable ways. Basic concepts of science,evolution and disease must also be considered. In this paper,I discuss some relevant problems associated with the application of any putative rejuvenation biotechnologies such as stem cell therapies,genetic engineering,tissue manipulation,as well as pharmacological approaches. I conclude that these and other biotechnologies will not be applicable to humans in the community. This is due to a wide spectrum of problems and obstacles,such as unpredictable therapeutic results,unrealistic expectations,lack of infrastructure,cellular network disruption,and many more. Even if some such technologies are developed,the totality of the problems,issues and side effects will prove an insurmountable final hurdle,rendering the development of such therapies,essentially and practically useless.

BIOTECHNOLOGY BENEFITS FARMERS ——A Brief Introduction of the Biotechnology Research Institute

The Biotechnology Research Institute (BRI), Chinese Academy of Agricultural Sciences, is one of the key institutions in China’s agricultural biotechnology area. There are 95 regular staff members and

香椿组培快繁效率的影响因子

香椿组织培养对基本培养基、激素种类以及浓度有较广泛的适应性,但不同的培养基及培养条件下,其快繁效率不同.通过对香椿用材品种茎段的离体培养,探讨了香椿组培快繁效率的影响因子.结果表明:香椿组培快繁效率高的诱导培养基为MS+6-BA0.5mg·L-1+NAA0.1～0.5mg·L-1,适宜分化丛生芽的理想培养基为4/5MS+6-BA0.5～1.0mg·L-1+NAA0.1mg·L-1+GA30.5mg·L-1,芽年增殖系数达5.612以上,有效芽苗率达100%,适合生根培养基为1/2MS+NAA0.5mg·L-1+GA30.1mg·L-1或1/2MS+IBA0.5mg·L-1+GA30.1mg·L-1,生根率达90%以上.移栽土壤基质以黄心土表面覆盖0.5～1.0cm厚细沙,移栽成活率可达90%以上.

Geminiviruses and their application in biotechnology

Being a major class of single-stranded DNA viruses, geminiviruses are mostly studied due to their catastrophic infectious effect on crops. These DNA viruses are characteristic for their ability in quickly multiplying viral genetic materials without integrating into the genome of plants, which makes them ideal for developing viral vectors for plants bioengineering. Geminivirus-derived vectors can be classified into expression vectors and virus-induced gene silencing（VIGS） vectors. Details of the design, construction, application and improvements of these geminivirus vectors are summarized and discussed.

The Use of Microbial Biotechnology in Value Addition to Cassava Wastes as Feed for Pigs: Prospects and Challenges

Cassava peels were fermented through a combination of two lactic acid bacteria (Lactobacillus coryneformis and Lactobacillus delbrueckii) and a fungus (Aspergillus fumigatus) using solid substrate fermentation (SSF) technique for five consecutive days. The enhanced product designated as microbially fermented cassava peel (MFCP) was used at graded levels in the feed formulation for growing pigs. Four iso-nitrogenous and iso-caloric diets were formulated with MFCP at 0% (T1), 20% (T2), 40% (T3) and 60% (T4) inclusion levels. Twenty-four boarlings of an average initial weight of 37.15 kg were used in a fourteen-week trial to test the effect of these graded levels of MFCP on growth performance, digestive and reproductive physiology of the experimental animals. The result showed significant differences (P < 0.05) in mean daily feed consumption. Pigs in T3 had the highest feed intake (2.24 ± 0.05 kg), while pigs in T4 had the lowest (1.78 ± 0.04 kg). The final highest live weight was in T3 (72.00 kg) and the least in T4 (58.17 kg). The feed conversion ratios also showed significant differences (P < 0.05) among treatment means. The result obtained from gut morphometry showed that animals in T1 had the highest stomach weight (full and empty), while animals in T3 had the least rate of gastric emptying (81.50%). Animals in T3 and T4 had the longest small intestine (16.21 m and 15.35 m, respectively), while those in T4 had the longest colon (4.24 m). Animals in T1 and T2 had more numbers of gastric glands, while animals in T3 and T4 had more parietal cells. The reproductive indices revealed that animals in T1 were better in terms of the weight and relative weight of vesicular and Cowper’s gland, while animals in T4 had the highest value of the weight and relative weight of prostate gland. Proximate analyses of the ileal digesta revealed that the rate of nutrient absorption was highest in T3 and T1 and lowest in T4. Histological examination of the testes revealed normal testicular architecture in the control diet. The testes of animals fed 0%, 20% and 40% MFCP diets exhibited normal and intact lumen, Sertoli cells, and germ cells. However, animal fed 60% MFCP diet revealed massive disruption and degeneration of germinal epithelium, and complete erosion of the seminiferous tubules. It could be concluded that feeding pigs at 40% MFCP would enhance feed consumption, nutrient absorption and improve the growth rate of pigs. Using MFCP beyond 40% level on swine diet could compromise gut health through diarrhoeagenic disposition of the digesta in the distal colon and modification of the parietal and chief cells of the gastric glands. The hypertrophy of the prostate gland and vacuolization of the seminiferous tubules of pigs fed the 60% MFCP diet indicated a probable reduced reproductive performance.

我国亚麻生物技术的研究现状及发展

本文简要介绍了国内外亚麻生产现状及发展,同时介绍了国内外基因工程、细胞工程的研究概况。从亚麻花药培养、单倍体育种、体细胞无性系突变体的利用、原生质体培养、转基因技术的研究等方面详细地介绍了我国亚麻生物技术的发展状况。从此看出了我国亚麻生物技术发展与国外先进水平的差距。但是我国在加大生物技术方面的资金投入的情况下,在亚麻花药培养、转基因技术、分子标记、基因克隆等方面开展深入的研究,一定能使我国亚麻生物技术实现跨越式发展,为我国亚麻生产的发展及经济的发展做出贡献。

Analysis on Development of Modern Biotechnology in Ethical Terms

In the paper, application of modem biotech was summarized and accompanying ethical problems were highlighted. In addition, four basic principles for modem biotech development were proposed.

Production of Biotechnology Fulvic Acid from Fermented Corn Straw by Microbial Community LCM9 and Its Application Effect

[Objective] The aim was to evaluate the feasibility of biotechnology fulvic acid produced by microbial community LCM9 by the use of corn straws as substrate and its application effect.[Method]Microbial community was obtained from the nature samples,and corn straws were used as fermentation substrate.The yield of biotechnology fulvic acid and its application effect was evaluated.[Result]The yield of biotechnology fulvic acid was up to 15.7% when the water content was 75.0% and fermented after 7 d using corn straws as the only source of nutrition.After 500 mg/kg of biotechnology fulvic acid was added for 5 d,the root length and bud length could be increased by 59.1% and 97.1%,respectively.[Conclusion]A higher yield of biotechnology fulvic acid could be obtained by the method in this experiment and the product could enhance the growth of wheat seedlings.