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Cloninger's Psychobiological Model of Temperament and the Discounting of Delayed and Effortful Outcomes Among Adolescents
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作者 Marta Malesza 《Psychology Research》 2015年第8期452-463,共12页
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Cloning and Expression of Rat Liver S-Adenosylmethionine Synthetase 被引量:1
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作者 CUI Wei HUANG Lei LI Wei WANG Li-ping 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2006年第2期236-238,共3页
The S-adenosylmethionine synthetase(SAM synthetase) is responsible for in vivo synthesis of S-adenosylmethionine(SAM), which is a kind of biologically active molecules distributed in all body tissues and fluids an... The S-adenosylmethionine synthetase(SAM synthetase) is responsible for in vivo synthesis of S-adenosylmethionine(SAM), which is a kind of biologically active molecules distributed in all body tissues and fluids and involved in a number of biochemical reactions. In this study, a cDNA containing the coding sequence for rat liver SAM synthetase was cloned into the prokaryotic expression vector pQE30 and expressed in E. coli M15. A major band corresponding to a protein of 48 kDa was detected on SDS-PAGE. The protein was distributed in both the soluble fraction and the insoluble fraction. In soluble fractions the protein was fully active. 展开更多
关键词 SAM SAM synthetase cloning EXPRESSION HPLC
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IDENTIFICATION OF A NEW HUMAN nm23 GENE,nm23-H3b
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作者 江贤朋 刘康达 +4 位作者 周信达 汤钊猷 张志芳 张颖 吴祥甫 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1994年第1期18-23,共6页
Nm23 is a kind of effective tumor metastasis suppressor genes which included two types in human:nm23-H1 and nm23-H2. Amino acid identity between nm23-H1 and nm23-H2 was 88%.In this study,using a pair of primers to fla... Nm23 is a kind of effective tumor metastasis suppressor genes which included two types in human:nm23-H1 and nm23-H2. Amino acid identity between nm23-H1 and nm23-H2 was 88%.In this study,using a pair of primers to flank the part of coding sequence of nm 23,the 5'-translated sequence was amplified by polymerase chain reaction (PCR) from human normal liver genomic DNA.A 375-base pairs clone was charactertzed,which designated pnm23-H3b.The nm23-H3b nucleotide sequence between 40 bp and 70 bp is different from nm23-H1 and nm23-H2,and other sequences have 86%and 90%identical to nm23-H1 and nm23-H2,respectively.Southern blot containing BglⅡ-digested human liver genomic DNA hybridized to the entire nm23-H3b DNA and showed three bands at 10.5,7.9 and 4.0 Kb.These data demonstrate that the third human nm23 exists possibly.Therefore,nm23 may be considered a family of closely related genes. 展开更多
关键词 Tumor metastasis suppressor gene nm23-H3b PCR cloning.
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Dolly,Cloning's Poster Child,Dies at Age 6
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作者 Maggie Fox 顾金凤 《当代外语研究》 2003年第3期1-3,共3页
选注者言:虽然全世界的吸引力在2月14日似乎都集中到了联合国武器核查小组负责人布利克斯向安理会提交关于伊拉克大规模杀伤性武器的第二份报告上,但是另一个消息或许更让人为之震动,或心有戚戚焉,或心存惋惜。世界上第一只用成年动物... 选注者言:虽然全世界的吸引力在2月14日似乎都集中到了联合国武器核查小组负责人布利克斯向安理会提交关于伊拉克大规模杀伤性武器的第二份报告上,但是另一个消息或许更让人为之震动,或心有戚戚焉,或心存惋惜。世界上第一只用成年动物体细胞克隆成功的哺乳动物——多利羊,在这一天被执行安乐死。多利的生,辉煌大于震惊,而多利的死,则震惊大于辉煌。 展开更多
关键词 Dolly cloning’s Poster Child Dies at Age 6
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