An estimated 73870 people will be diagnosed with melanoma in the United States in 2015,resulting in 9940 deaths.The majority of patients with cutaneous melanomas are cured with wide local excision.However,current evid...An estimated 73870 people will be diagnosed with melanoma in the United States in 2015,resulting in 9940 deaths.The majority of patients with cutaneous melanomas are cured with wide local excision.However,current evidence supports the use of sentinel lymph node biopsy(SLNB) given the 15%-20% of patients who harbor regional node metastasis.More importantly,the presence or absence of nodal micrometastases has been found to be the most important prognostic factor in earlystage melanoma,particularly in intermediate thickness melanoma.This review examines the development of SLNB for melanoma as a means to determine a patient's nodal status,the efficacy of SLNB in patients with melanoma,and the biology of melanoma metastatic to sentinel lymph nodes.Prospective randomized trials have guided the development of practice guidelines for use of SLNB for melanoma and have shown the prognostic value of SLNB.Given the rapidly advancing molecular and surgical technologies,the technical aspects of diagnosis,identification,and management of regional lymph nodes in melanoma continues to evolve and to improve.Additionally,there is ongoing research examining both the role of SLNB for specific clinical scenarios and the ways to identify patients who may benefit from completion lymphadenectomy for a positive SLN.Until further data provides sufficient evidence to alter national consensusbased guidelines,SLNB with completion lymphadenectomy remains the standard of care for clinically node-negative patients found to have a positive SLN.展开更多
This article represents the main positions of the theory of pleiotropic action of biologically active compounds (BACs) and medicines, which has been designed by the author based on her own experimental researches. The...This article represents the main positions of the theory of pleiotropic action of biologically active compounds (BACs) and medicines, which has been designed by the author based on her own experimental researches. The term “pleiotropy” means the ability of the BACs and medicines to implement more than one mechanism of action resulting in the specific biological (pharmacological) effect. The interaction of these mechanisms forms a distinct pattern of biological response (pleiotropic pattern), which reflects the change in his character with the increased dose (concentration)-dependent efficacy of BACs and medicines. The article consists of description of different pleiotropic patterns established in experiments on the model of reactive oxygen species (ROS) generation by macrophages dependent on activity of specialized enzyme called Nox2-NAD(P)H oxidase (Nox2, EC 1.6.3.1). Moreover, it consists of explanation of pharmacodynamic nature of pleiotropic patterns by means of application Chou-Talalay median effect equalization and combination index (CI) theory. The novel theory explains unsolved until now universal aspects of activity BACs and medicines, such as slope angles of “dose-effect” dependences in the conditions relevant in vivo, and it is of fundamental interest. However, it has applications in experimental pharmacology, as it allows defining the choice of the individual compounds and combinations, modulating the trust effect selectively and efficiently. This knowledge opens up new approaches to medicines discovery and evaluation, their rational dosing and combining.展开更多
The morphological and cultural characteristics of Hendersonia acicola Munch & Tub,, isolated from the needles of Pinus sylvestris var. mongolica Litvin, were described. The fungi was cultured on three kinds of med...The morphological and cultural characteristics of Hendersonia acicola Munch & Tub,, isolated from the needles of Pinus sylvestris var. mongolica Litvin, were described. The fungi was cultured on three kinds of media and the speed of mycelial growth and the required time to produce conidia were different. On the medium of needle decoction agar, the fungi grew fast and produced conidia after 5 days culturing. On PDA came second and it needed 7 days to produce conidia. On the medium of maize flour agar, it grew slowly and produces conidia after 27 days incubating. The fungi cannot produce conidia under darkness on all three kinds of media.The conidia can germinate at 8℃ -28℃. The optimum conditions for conidia to germinate are 25℃, RH 100% and pH 4 and under these conditions it only takes four hours for conidia to germinate. The conidia germination is not affected by展开更多
[Objective] The biological characteristics and pathogenicities of Shewanella algae and Shewanella abalone from Babylonia were studied in this paper. [Method]The hemolytic bacteria were isolated from the hepatopancreas...[Objective] The biological characteristics and pathogenicities of Shewanella algae and Shewanella abalone from Babylonia were studied in this paper. [Method]The hemolytic bacteria were isolated from the hepatopancreas of Babylonia suffered from proboscis edema with blood agar plate. The dominant bacterial community in the ill Babylonia was identified by 16 S r DNA sequence analysis, and the bacterial cultural and biochemical characteristics and pathogenicities were studied. [Result]The Shewanella bacteria, including Shewanella algae and Shewanella abalone, are the dominant bacterial community in Babylonia suffered from proboscis edema.The colony characteristics of Shewanella algae in nutrient agar medium, TCBS agar medium and CHROMagar vibrio colored medium were similar to those of Shewanella abalone. Shewanella algae possessed β-hemolysis and Shewanella abalone possessed α-hemolysis in the blood agar plate. The biochemical reaction of Shewanella algae and Shewanella abalone was all of non-fermentation type. The results of artificial infection test showed that half lethal dose(LD50) of the test strains of Shewanella algae was 10-5.50/0.1 ml. The test strains of Shewanella algae have strong toxicity, and could cause mice and chickens to die of sepsis with mortality of100%. The mortality of Babylonia infected with Shewanella algae was 10%; while the survived Babylonia lost the ability of moving and intaking for a long time, but they were not suffered from proboscis edema. There was no death in mice or chicks infected with Shewanella abalone, but their livers and spleens were slightly hyperemic and swelling. There was also no death in Babylonia infected with Shewanella abalone, but their intaking and moving ability was lost for a short time.[Conclusion] Although Shewanella algae and Shewanella abalone were the dominant bacteria in Babylonia suffered from proboscis edema, they were not the main pathogenic bacteria for proboscis edema. Shewanella algae had strong pathogenicity to mice, chicks and Babylonia, while Shewanella abalone showed no marked pathogenicity to those experimental animals in this study.展开更多
Studies indicat that; 1. when soil moisture is above 15% and soil temperature is 20-28℃ ,the condition is benefic for infecting and reproducing of the nematodes. At the same time,this condition is samely needed for t...Studies indicat that; 1. when soil moisture is above 15% and soil temperature is 20-28℃ ,the condition is benefic for infecting and reproducing of the nematodes. At the same time,this condition is samely needed for the white grubs; 2. the migration ability of the nematodes is lower in soil of field than that in tested soil of the laboratory. But in general,the nematodes appplyed into soil distribute mainly in the ploughed layer and a migration area for them can be formed in the direction of horizontal; 3. observations reveal that invasion time of the S. glaseri NC34 into larval (H. parallela) through intersegmental membrane of body segment and foot after the inoculation is 30 min,and it for invading cuticle of larvae body side is 120 min. But the infection time is different in the different individuals of S. glaseri and in the different species of grubs.展开更多
This paper reviewed the unique biological function of trehalose and its mechanism of stabilizing biological macromolecules and the research progress in the protective effect of trehalose on lactic acid bacteria fermen...This paper reviewed the unique biological function of trehalose and its mechanism of stabilizing biological macromolecules and the research progress in the protective effect of trehalose on lactic acid bacteria fermentation starters during lyophilization in food production.The application of trehalose in food industry was prospected.展开更多
Objective:To construct the tissue engineering seed cell(HaCaT cell line) with stable expression of the human epidermal growth factor(EGF),and analyze the changes of its biological characteristics.Methods:PCDNA3.1-EGF ...Objective:To construct the tissue engineering seed cell(HaCaT cell line) with stable expression of the human epidermal growth factor(EGF),and analyze the changes of its biological characteristics.Methods:PCDNA3.1-EGF eukaryotic expression vector was transferred into HaCaT cell,and G418 was utilized to select the HaCaT-EGF cell line.Using an inverted microscope,PCR,ELISA method to detect the changes of the cell morphology,the expression of the ECF gene and protein,and the mRNA expression levels of apoptosis related molecule Caspase-3,the cell cycle related protein cyclin D1.Results:The mRNA expression levels of the obtained HaCaT-ECF cell were more than 100 times higher than the level of ordinary HaCaT cell.The colony of the HaCaT-EGF cells was more focused and tight compared to the empty vector transfected HaCaT cells and normal HaCaT cells.The expression levels of apoptotic factor Caspase-3 and cyclin Dt in HaCaT-EGF cell were significantly higher than those in the empty vector HaCaT- pcDNA3.1 cell,and the differences were statistically significant(P<0.01),but there was no significant difference compared to the normal HaCaT cells(P>0.05).Conclusions: HaCaT-EGF cell can continuously secrete ECF,and the biological characteristic is stable.It can be used for tissue engineering experiment and is an ideal seed cell for constructing tissue engineered skin.展开更多
OBJECTIVE: To summarize the biological characteristics of neural stem cells, and the separation, purification. differentiation and source of neural stem cells. DATA SOURCES : An online search of Pubmed database was ...OBJECTIVE: To summarize the biological characteristics of neural stem cells, and the separation, purification. differentiation and source of neural stem cells. DATA SOURCES : An online search of Pubmed database was undertaken to identify English articles about the growth of neural stem cells in vitro published from January 2000 to October 2006 by using the keywords of "neural stem cells, bone marrow mesenchymal stem cells (BMSCs), umbilical cord blood stem cells, embryonic stem cells (ESC), separation methods, neural growth factor". And relevant articles published in IEEE/IEE Electronic Library (IEL) database, Springer Link database and Kluwer Online Journals were also searched, Chinese relevant articles published between January 2000 to October 2006 were searched with the same keywords in Chinese in Chinese journal full-text database. STUDY SELECTION : The articles were primarily screened, and then the full-texts were searched. Inclusive criteria: (1) Articles relevant to the biological characteristics and classification of neural stem cells; (2) Articles about the source, separation and differentiation of the ESCs, BMSCs and umbilical cord blood stem cells. The repetitive studies and reviews were excluded. DATA EXTRACTION : Thirty articles were selected from 203 relevant articles according to the inclusive criteria Articles were excluded because of repetition and reviews. DATA SYNTHESES : Neural stem cells have the ability of self-renewing and high differentiation, and they are obtained from ESCs, nerve tissue, nerve system, BMSCs and umbilical cord blood stem cells. ESCs can be separated by means of mechanical dissociation is better than that of the trypsin digestion, BMSCs by density gradient centrifuge separation, hemolysis, whole-blood culture, etc., and umbilical cord blood stem ceils by Ficoil density gradient centrifugation, hydroxyethyl starch (HES) centrifugation sedimentation, etc. Neural growth factor (NGF) and other factors play an important role in the growth of NSCs, such as transforming growth factor (TGF) is an important player in repairing organs, NGF accelerates the process of growth, insulin-like growth factor serves importantly in the differentiation of stem cells into neuron-like cells. CONCLUSION : As unipotent stem cells, NSCs have the abilities of self-renewal and potential of high differentiation. The method of mechanical dissociation is better than trypsin digestion in e separating ESCs. However, density gradient centrifuge separation is better than other methods in the separation of the BMSCs. NGF and other factors play an important role in the growth of NSCs.展开更多
[Objective] This paper was aimed to study the biological characteristics of Colletotrichum orbiculare(Berk.et Mont.) Arx, and accomplish the preliminary screening the test of fungicides. [Method]The pathogen was iso...[Objective] This paper was aimed to study the biological characteristics of Colletotrichum orbiculare(Berk.et Mont.) Arx, and accomplish the preliminary screening the test of fungicides. [Method]The pathogen was isolated from the infected leaves of watermelon collected from greenhouse in Hainan Province. The biological characteristics of C. orbiculare and indoor toxicity of fungicides were studied. [Results] The mycelium grew fastest on the medium with leaf extract of watermelon. The optimum temperature for mycelial growth and spore germination was 28 and 30 ℃, and the optimum pH was 7-9 and 6-8, respectively. The continuous darkness condition was most suitable for mycelial growth. The lethal temperature of spore was 55 ℃ water bath for 10 min in water bath. The suitable carbon sources were maltose, L-arabinose and glucose, and the suitable nitrogen sources were yeast extract, beef extract and peptone. In the indoor toxicity determination, 32.5% Benzoic azoxystrobin SC, 50% Dichlorotetrakis WP and 10% Difenoconazole WG showed better inhibition effects against the mycelial growth of C. orbiculare, with the EC50 values of 0.018 0, 0.060 2 and 0.471 5 μg/ml, respectively. [Conclusion]The research provided theoretical basis for the control of watermelon anthracnose in watermelon fields.展开更多
Na^(+)/K^(+)-ATPase is a transmembrane protein that has important roles in the maintenance of electrochemical gradients across cell membranes by transporting three Na^(+)out of and two K^(+)into cells.Additionally,Na^...Na^(+)/K^(+)-ATPase is a transmembrane protein that has important roles in the maintenance of electrochemical gradients across cell membranes by transporting three Na^(+)out of and two K^(+)into cells.Additionally,Na^(+)/K^(+)-ATPase participates in Ca^(2+)-signaling transduction and neurotransmitter release by coordinating the ion concentration gradient across the cell membrane.Na^(+)/K^(+)-ATPase works synergistically with multiple ion channels in the cell membrane to form a dynamic network of ion homeostatic regulation and affects cellular communication by regulating chemical signals and the ion balance among different types of cells.Therefo re,it is not surprising that Na^(+)/K^(+)-ATPase dysfunction has emerged as a risk factor for a variety of neurological diseases.However,published studies have so far only elucidated the important roles of Na^(+)/K^(+)-ATPase dysfunction in disease development,and we are lacking detailed mechanisms to clarify how Na^(+)/K^(+)-ATPase affects cell function.Our recent studies revealed that membrane loss of Na^(+)/K^(+)-ATPase is a key mechanism in many neurological disorders,particularly stroke and Parkinson's disease.Stabilization of plasma membrane Na^(+)/K^(+)-ATPase with an antibody is a novel strategy to treat these diseases.For this reason,Na^(+)/K^(+)-ATPase acts not only as a simple ion pump but also as a sensor/regulator or cytoprotective protein,participating in signal transduction such as neuronal autophagy and apoptosis,and glial cell migration.Thus,the present review attempts to summarize the novel biological functions of Na^(+)/K^(+)-ATPase and Na^(+)/K^(+)-ATPase-related pathogenesis.The potential for novel strategies to treat Na^(+)/K^(+)-ATPase-related brain diseases will also be discussed.展开更多
[ Objective] The paper was to understand the biological characteristics of bananamoth [ Opogona sacchari ( Bojer ) ] larvae in sugarcane. [ Method ] Through the rearing test of bananamoth larvae with different hosts...[ Objective] The paper was to understand the biological characteristics of bananamoth [ Opogona sacchari ( Bojer ) ] larvae in sugarcane. [ Method ] Through the rearing test of bananamoth larvae with different hosts, the biological characteristics of bananamoth larvae in sugarcane were observed and analyzed. [ Result ] The mortality rate of bananamoth larvae reared with sugarcane was higher than that of the pests reared with potato. The larva had 8 instars, and the devel- opment duration of the larvae reared with sugarcane was 34.6 d longer than the pests reared with potato. The larvae needed about 98.8 d to complete a generation in average. Viewed from the observation values of head width, body length and weight of larvae, bananamoth larvae reared with sugarcane basically accorded with the growth and development law of larvae. [Condusion ] The results provided theoretical basis for the preparation of effective measures against bananamoth and the assurance of safe production of.sugarcane.展开更多
[Objective] In order to find the pathogenic microorganisms suitable for biological control of filth flies, the pathogenic microorganism was isolated from the dead fly, Boettcherisca peregrine. [Method] The conidia and...[Objective] In order to find the pathogenic microorganisms suitable for biological control of filth flies, the pathogenic microorganism was isolated from the dead fly, Boettcherisca peregrine. [Method] The conidia and mycelia were observed by optical microscope. The pathogenic microorganism was identified on the basis of its culture characters and the optical morphologies of the conidia and mycelia, and its biological characteristics and pathogenicity were preliminarily studied. [Result] The pathogenic microorganism isolated from the dead fly was a new strain of Verticillium lecanii. The new strain of V. lecanii was numbered as KMZW-1. The colonies of V. lecanii KMZW-1 grew fastest on potato dextrose agar medium (PDA) at 29 ℃ and pH 6.0. The LC50 of its spore suspension to the adults of B. peregrine, Lucilia sericata, Musca domestic, Piophila casei and Drosophila melanogaster were 9.50×10^5, 4.58×10^7, 4.06×10^7, 4.10×10^3 and 1.05×10^7 conidia/ml, respectively. The LT50 were 6.86, 8.17, 8.16, 8.12 and 3.22 d, respectively. [Conclusion] V. lecanii KMZW-1 is an active pathogenic microorganism to control the adults of five fly species.展开更多
Reliable and non-invasive diagnostic tools are highly valuable for successful therapeutic strategies for the treatment of Alzheimer's disease(AD). The existence of neurofibrillary tangles(NFTs) consisting of tau p...Reliable and non-invasive diagnostic tools are highly valuable for successful therapeutic strategies for the treatment of Alzheimer's disease(AD). The existence of neurofibrillary tangles(NFTs) consisting of tau protein are one kind of the pathological features of AD, and its level of severity is correlated with the stage of AD.However, no clinically approved positron emission tomography(PET) probe is currently available for selective imaging of neurofibrillary tangles on patients. In this paper, we report our studies on biological characteristics of [18F]-THK523 as a novel tau imaging probe. With low molecular weight, [18F]-THK523 is stable, electrically neutral, lipophilic and non-mass concentration-dependent. Preliminary biological studies have shown the excellent properties of [18F]-THK523 as brain imaging tracer for further research.展开更多
AIM: To detect the biological characters of the SGC7901 gastric cancer cell-dendritic cell fusion vaccines.METHODS: The suspending living SGC7901 gastric cancer cells and dendritic cells were induced to be fusioned ...AIM: To detect the biological characters of the SGC7901 gastric cancer cell-dendritic cell fusion vaccines.METHODS: The suspending living SGC7901 gastric cancer cells and dendritic cells were induced to be fusioned by polyethylene glycol. Pure fusion cells were obtained by selective culture with the HAT/HT culture systems. The fusion cells were counted at different time points of culture and their growth curves were drawn to reflect their proliferative activities. The fusion cells were also cultured in culture medium to investigate whether they could grow into cell clones. MTT method was used to test the stimulating abilities of the fusion cells on T lymphocytes' proliferations. Moreover, the fusion cells were planted into nude mice to observe whether they could grow into new planted tumors in this kind of immunodeficiency animals.RESULTS: The fusion cells had weaker proliferative activity and clone abilities than their parental cells. When they were cultured, the counts of cells did not increase remarkably, nor could they grow into cell clones in culture medium. The fusion cells could not grow into new planted tumors after planted into nude mice. The stimulating abilities of the fusion cells on T lymphocytes' proliferations were remarkably increased than their parental dendritic cells. CONCLUSION: The SGC7901 gastric cancer cell-dendritic cell fusion vaccines have much weaker proliferative abilities than their parental cells, but they keep strong abilities to irritate the T lymphocytes and have no abilities to grow into new planted tumors in immunodeficiency animals. These are the biological basis for their antitumor biotherapies.展开更多
[ Objective ] The study systematically studied the biological characteristics of ginseng Botrytis cinerea Pers. [ Method ] The pure pathogenic fungus was isolated from ginseng B. cinerea collected in the field by tiss...[ Objective ] The study systematically studied the biological characteristics of ginseng Botrytis cinerea Pers. [ Method ] The pure pathogenic fungus was isolated from ginseng B. cinerea collected in the field by tissue segregation and purification cultivation. Subsequently, using PDA medium plate culture method, the effect of various culture conditions on mycelium growth and sporulation of ginseng B. cinerea was detected. [ Result] The optimum temperature for mycelium growth and sporulation of B. cinerea was 25 ℃. The appropriate temperature for conidia germination ranged from 20 to 25 ℃. And the optimum pH value for mycelium growth and conidia germination was 6.0. The optimum carbon source was sucrose, followed by glucose and fructose. The optimum nitrogen source was peptone, fol- lowed by beef extract, yeast extract, alanine and ammonium nitrate. Among the media, the growth of mycelium cultured on PDA medium was the fastest with the production of gray mycelium and dense colonies. Lethal temperatures for sclerotia, mycelium and conidia were 60, 55 and 50 ℃, respectively. [ Conclusion] The study provided the scientific basis for the research on the incidence law of B. cinerea and its control.展开更多
The growth characteristics of petroleum-degrader BS-8(Bacillus sp.) and the factors influencing its biosurfactant production were tested; the biosurfactant releasing mode of BS-8 was speculated by measuring OD600, s...The growth characteristics of petroleum-degrader BS-8(Bacillus sp.) and the factors influencing its biosurfactant production were tested; the biosurfactant releasing mode of BS-8 was speculated by measuring OD600, surface tension and oil displacement of fermentation broth; and the effects of carbon source, nitrogen source, temperature, p H, and Na Cl concentration on biosurfactant production by BS-8 were observed in this study. The results showed that the biosurfactant releasing mode of BS-8 was growth-correlated, the surface tension of BS-8 fermentation broth declined with the total bacteria increasing, and the oil displacement was positive correlated with biosurfactant content in fermentation broth; and the optimal culture conditions for effective biosurfactant production included: glucose as carbon source,yeast extract as nitrogen source, Na Cl concentration of 2%, p H of 7.0 and temperature at 30 ℃.展开更多
The presented paper is dedicated to a new ret-rospective view on the history of natural sci-ences in XX-XXI cc, partially including the sci-ence philosophy (mainly, the problems of the scientific realism, i.e. the cor...The presented paper is dedicated to a new ret-rospective view on the history of natural sci-ences in XX-XXI cc, partially including the sci-ence philosophy (mainly, the problems of the scientific realism, i.e. the correspondence of science to reality) and also a novel scheme for different classes of sciences with different ob-jects and paradigms. There are analyzed the chosen “great” and “grand” problems of phys-ics (including the comprehension of quantum mechanics, with a recently elaborated new chapter, connected with time as a quantum obs- ervable and time analysis of quantum processes) and also of natural sciences as a whole. The particular attention is paid to the interpretation questions and slightly to the aspects, inevitably connected with the world- views of the res- earchers (which do often constitute a part of the interpretation questions).展开更多
[Objectives]To study the biological characteristics and culture conditions of Hericium coralloides(Scop.)Pers.[Methods]The wild H.coralloides strain of Changbai Mountain was used as the experimental material to conduc...[Objectives]To study the biological characteristics and culture conditions of Hericium coralloides(Scop.)Pers.[Methods]The wild H.coralloides strain of Changbai Mountain was used as the experimental material to conduct a single factor test on its biological characteristics.[Results]The ideal available carbon source and nitrogen source are glucose and ammonium sulfate,respectively,the pH is 3.5-4.0,and the temperature is 20-22℃.Through the orthogonal experiment of 4 factors and 4 levels,it is found that the degree of influence of the 4 factors on H.coralloides is temperature>pH>nitrogen source>carbon source.[Conclusions]The optimum conditions for the mycelium culture of H.coralloides:the carbon source is glucose,and the nitrogen source is yeast extract or peptone,and pH is 3.5-4.0,and temperature is 20-22℃.展开更多
Gypsy moth, Lymantria dispar (L.) is one of the most widespread defoliators of deciduous and larch forests in Kazakhstan. Preferred host plants, flying capacity of females and neonate caterpillars, preferred places ...Gypsy moth, Lymantria dispar (L.) is one of the most widespread defoliators of deciduous and larch forests in Kazakhstan. Preferred host plants, flying capacity of females and neonate caterpillars, preferred places for egg laying, major factors of mortality and other characteristics vary considerably between different populations of the pest. According to several year researches on gypsy moth, in Kazakhstan, the main biological characteristics of this pest in the regions were investigated. Results of research showed that gypsy moth exists in central and northern of theses regions. From biological and geographical aspects, gypsy moth which there is in Kazakhstan is close to the same species from Siberia western regions. The pest lays on the different heights of trees trunk. It was found that the number of eggs in each egg mass and egg masses laid by the pest are different. The lack of egg eaters and the over- reproduction of the pest at the natural conditions have resulted in the development of bacterial and viral diseases in the populations of this pest from the regions of Kazakhstan to Siberia west.展开更多
[Objective] This study was to investigate the relationship between biological characteristics of Beauveria bassiana (Bals.) Vuill and pathogenicity to Bombyx rnori L, with the aim to provide scientific basis for the...[Objective] This study was to investigate the relationship between biological characteristics of Beauveria bassiana (Bals.) Vuill and pathogenicity to Bombyx rnori L, with the aim to provide scientific basis for the control of white muscardine in Bombyx mori L. [Method] The strains isolated and purified from the 6 Beauveria bassiana biocontrol agents from all over the country and the 3 white muscardine silkworms collected from Guangxi provincial silkworm rearing areas were identified by the morphological observation and molecular biology technology. The pathogenicity of B. bassaina to silkworms was determined, and the biological characteristics such as growth diameter, sporulation and the extracellular protease activity of the different B. bassiana strains were compared. [Result] The isolated 9 strains were all B. bassaina (Bals.) Vuillemin, and all strains had high pathogenicity to silkworm, but with different pathogenicities. The growth diameter, sporulation and extracellular protease activity of different B. bassiana strains were also different, and showed correlation with the patheogenicity to silkworms. [Conclusion] B. bassiana spores production amount and exocellular protease activity had significant positive correlation with their pathogenicity to silkworm.展开更多
文摘An estimated 73870 people will be diagnosed with melanoma in the United States in 2015,resulting in 9940 deaths.The majority of patients with cutaneous melanomas are cured with wide local excision.However,current evidence supports the use of sentinel lymph node biopsy(SLNB) given the 15%-20% of patients who harbor regional node metastasis.More importantly,the presence or absence of nodal micrometastases has been found to be the most important prognostic factor in earlystage melanoma,particularly in intermediate thickness melanoma.This review examines the development of SLNB for melanoma as a means to determine a patient's nodal status,the efficacy of SLNB in patients with melanoma,and the biology of melanoma metastatic to sentinel lymph nodes.Prospective randomized trials have guided the development of practice guidelines for use of SLNB for melanoma and have shown the prognostic value of SLNB.Given the rapidly advancing molecular and surgical technologies,the technical aspects of diagnosis,identification,and management of regional lymph nodes in melanoma continues to evolve and to improve.Additionally,there is ongoing research examining both the role of SLNB for specific clinical scenarios and the ways to identify patients who may benefit from completion lymphadenectomy for a positive SLN.Until further data provides sufficient evidence to alter national consensusbased guidelines,SLNB with completion lymphadenectomy remains the standard of care for clinically node-negative patients found to have a positive SLN.
文摘This article represents the main positions of the theory of pleiotropic action of biologically active compounds (BACs) and medicines, which has been designed by the author based on her own experimental researches. The term “pleiotropy” means the ability of the BACs and medicines to implement more than one mechanism of action resulting in the specific biological (pharmacological) effect. The interaction of these mechanisms forms a distinct pattern of biological response (pleiotropic pattern), which reflects the change in his character with the increased dose (concentration)-dependent efficacy of BACs and medicines. The article consists of description of different pleiotropic patterns established in experiments on the model of reactive oxygen species (ROS) generation by macrophages dependent on activity of specialized enzyme called Nox2-NAD(P)H oxidase (Nox2, EC 1.6.3.1). Moreover, it consists of explanation of pharmacodynamic nature of pleiotropic patterns by means of application Chou-Talalay median effect equalization and combination index (CI) theory. The novel theory explains unsolved until now universal aspects of activity BACs and medicines, such as slope angles of “dose-effect” dependences in the conditions relevant in vivo, and it is of fundamental interest. However, it has applications in experimental pharmacology, as it allows defining the choice of the individual compounds and combinations, modulating the trust effect selectively and efficiently. This knowledge opens up new approaches to medicines discovery and evaluation, their rational dosing and combining.
文摘The morphological and cultural characteristics of Hendersonia acicola Munch & Tub,, isolated from the needles of Pinus sylvestris var. mongolica Litvin, were described. The fungi was cultured on three kinds of media and the speed of mycelial growth and the required time to produce conidia were different. On the medium of needle decoction agar, the fungi grew fast and produced conidia after 5 days culturing. On PDA came second and it needed 7 days to produce conidia. On the medium of maize flour agar, it grew slowly and produces conidia after 27 days incubating. The fungi cannot produce conidia under darkness on all three kinds of media.The conidia can germinate at 8℃ -28℃. The optimum conditions for conidia to germinate are 25℃, RH 100% and pH 4 and under these conditions it only takes four hours for conidia to germinate. The conidia germination is not affected by
基金Supported by Special Project for Marine Fisheries Science and Technology and Industrial Development of Guangdong Province(A201508A05)Regional Demonstration Project of Marine Economy Innovation and Development of Guangdong Province(GD2012-A03-012)~~
文摘[Objective] The biological characteristics and pathogenicities of Shewanella algae and Shewanella abalone from Babylonia were studied in this paper. [Method]The hemolytic bacteria were isolated from the hepatopancreas of Babylonia suffered from proboscis edema with blood agar plate. The dominant bacterial community in the ill Babylonia was identified by 16 S r DNA sequence analysis, and the bacterial cultural and biochemical characteristics and pathogenicities were studied. [Result]The Shewanella bacteria, including Shewanella algae and Shewanella abalone, are the dominant bacterial community in Babylonia suffered from proboscis edema.The colony characteristics of Shewanella algae in nutrient agar medium, TCBS agar medium and CHROMagar vibrio colored medium were similar to those of Shewanella abalone. Shewanella algae possessed β-hemolysis and Shewanella abalone possessed α-hemolysis in the blood agar plate. The biochemical reaction of Shewanella algae and Shewanella abalone was all of non-fermentation type. The results of artificial infection test showed that half lethal dose(LD50) of the test strains of Shewanella algae was 10-5.50/0.1 ml. The test strains of Shewanella algae have strong toxicity, and could cause mice and chickens to die of sepsis with mortality of100%. The mortality of Babylonia infected with Shewanella algae was 10%; while the survived Babylonia lost the ability of moving and intaking for a long time, but they were not suffered from proboscis edema. There was no death in mice or chicks infected with Shewanella abalone, but their livers and spleens were slightly hyperemic and swelling. There was also no death in Babylonia infected with Shewanella abalone, but their intaking and moving ability was lost for a short time.[Conclusion] Although Shewanella algae and Shewanella abalone were the dominant bacteria in Babylonia suffered from proboscis edema, they were not the main pathogenic bacteria for proboscis edema. Shewanella algae had strong pathogenicity to mice, chicks and Babylonia, while Shewanella abalone showed no marked pathogenicity to those experimental animals in this study.
文摘Studies indicat that; 1. when soil moisture is above 15% and soil temperature is 20-28℃ ,the condition is benefic for infecting and reproducing of the nematodes. At the same time,this condition is samely needed for the white grubs; 2. the migration ability of the nematodes is lower in soil of field than that in tested soil of the laboratory. But in general,the nematodes appplyed into soil distribute mainly in the ploughed layer and a migration area for them can be formed in the direction of horizontal; 3. observations reveal that invasion time of the S. glaseri NC34 into larval (H. parallela) through intersegmental membrane of body segment and foot after the inoculation is 30 min,and it for invading cuticle of larvae body side is 120 min. But the infection time is different in the different individuals of S. glaseri and in the different species of grubs.
基金Supported by School-based Scientific Research Project of Hebi Polytechnic in 2022 (2022-KJZD-001).
文摘This paper reviewed the unique biological function of trehalose and its mechanism of stabilizing biological macromolecules and the research progress in the protective effect of trehalose on lactic acid bacteria fermentation starters during lyophilization in food production.The application of trehalose in food industry was prospected.
基金This research work was funded by the National Natural Science Foundation of China(Grant No.30901562)
文摘Objective:To construct the tissue engineering seed cell(HaCaT cell line) with stable expression of the human epidermal growth factor(EGF),and analyze the changes of its biological characteristics.Methods:PCDNA3.1-EGF eukaryotic expression vector was transferred into HaCaT cell,and G418 was utilized to select the HaCaT-EGF cell line.Using an inverted microscope,PCR,ELISA method to detect the changes of the cell morphology,the expression of the ECF gene and protein,and the mRNA expression levels of apoptosis related molecule Caspase-3,the cell cycle related protein cyclin D1.Results:The mRNA expression levels of the obtained HaCaT-ECF cell were more than 100 times higher than the level of ordinary HaCaT cell.The colony of the HaCaT-EGF cells was more focused and tight compared to the empty vector transfected HaCaT cells and normal HaCaT cells.The expression levels of apoptotic factor Caspase-3 and cyclin Dt in HaCaT-EGF cell were significantly higher than those in the empty vector HaCaT- pcDNA3.1 cell,and the differences were statistically significant(P<0.01),but there was no significant difference compared to the normal HaCaT cells(P>0.05).Conclusions: HaCaT-EGF cell can continuously secrete ECF,and the biological characteristic is stable.It can be used for tissue engineering experiment and is an ideal seed cell for constructing tissue engineered skin.
文摘OBJECTIVE: To summarize the biological characteristics of neural stem cells, and the separation, purification. differentiation and source of neural stem cells. DATA SOURCES : An online search of Pubmed database was undertaken to identify English articles about the growth of neural stem cells in vitro published from January 2000 to October 2006 by using the keywords of "neural stem cells, bone marrow mesenchymal stem cells (BMSCs), umbilical cord blood stem cells, embryonic stem cells (ESC), separation methods, neural growth factor". And relevant articles published in IEEE/IEE Electronic Library (IEL) database, Springer Link database and Kluwer Online Journals were also searched, Chinese relevant articles published between January 2000 to October 2006 were searched with the same keywords in Chinese in Chinese journal full-text database. STUDY SELECTION : The articles were primarily screened, and then the full-texts were searched. Inclusive criteria: (1) Articles relevant to the biological characteristics and classification of neural stem cells; (2) Articles about the source, separation and differentiation of the ESCs, BMSCs and umbilical cord blood stem cells. The repetitive studies and reviews were excluded. DATA EXTRACTION : Thirty articles were selected from 203 relevant articles according to the inclusive criteria Articles were excluded because of repetition and reviews. DATA SYNTHESES : Neural stem cells have the ability of self-renewing and high differentiation, and they are obtained from ESCs, nerve tissue, nerve system, BMSCs and umbilical cord blood stem cells. ESCs can be separated by means of mechanical dissociation is better than that of the trypsin digestion, BMSCs by density gradient centrifuge separation, hemolysis, whole-blood culture, etc., and umbilical cord blood stem ceils by Ficoil density gradient centrifugation, hydroxyethyl starch (HES) centrifugation sedimentation, etc. Neural growth factor (NGF) and other factors play an important role in the growth of NSCs, such as transforming growth factor (TGF) is an important player in repairing organs, NGF accelerates the process of growth, insulin-like growth factor serves importantly in the differentiation of stem cells into neuron-like cells. CONCLUSION : As unipotent stem cells, NSCs have the abilities of self-renewal and potential of high differentiation. The method of mechanical dissociation is better than trypsin digestion in e separating ESCs. However, density gradient centrifuge separation is better than other methods in the separation of the BMSCs. NGF and other factors play an important role in the growth of NSCs.
基金Supported by Scientific Funds Project of Hainan Province[(QCY(2009)71]Local Standards of Hainan Province in 2011 "Technical Regulation of Greenhouse Watermelon Production"Project[QZJJ(2011)11]
文摘[Objective] This paper was aimed to study the biological characteristics of Colletotrichum orbiculare(Berk.et Mont.) Arx, and accomplish the preliminary screening the test of fungicides. [Method]The pathogen was isolated from the infected leaves of watermelon collected from greenhouse in Hainan Province. The biological characteristics of C. orbiculare and indoor toxicity of fungicides were studied. [Results] The mycelium grew fastest on the medium with leaf extract of watermelon. The optimum temperature for mycelial growth and spore germination was 28 and 30 ℃, and the optimum pH was 7-9 and 6-8, respectively. The continuous darkness condition was most suitable for mycelial growth. The lethal temperature of spore was 55 ℃ water bath for 10 min in water bath. The suitable carbon sources were maltose, L-arabinose and glucose, and the suitable nitrogen sources were yeast extract, beef extract and peptone. In the indoor toxicity determination, 32.5% Benzoic azoxystrobin SC, 50% Dichlorotetrakis WP and 10% Difenoconazole WG showed better inhibition effects against the mycelial growth of C. orbiculare, with the EC50 values of 0.018 0, 0.060 2 and 0.471 5 μg/ml, respectively. [Conclusion]The research provided theoretical basis for the control of watermelon anthracnose in watermelon fields.
基金supported by the National Natural Science Foundation of China,No.82173800 (to JB)Shenzhen Science and Technology Program,No.KQTD20200820113040070 (to JB)。
文摘Na^(+)/K^(+)-ATPase is a transmembrane protein that has important roles in the maintenance of electrochemical gradients across cell membranes by transporting three Na^(+)out of and two K^(+)into cells.Additionally,Na^(+)/K^(+)-ATPase participates in Ca^(2+)-signaling transduction and neurotransmitter release by coordinating the ion concentration gradient across the cell membrane.Na^(+)/K^(+)-ATPase works synergistically with multiple ion channels in the cell membrane to form a dynamic network of ion homeostatic regulation and affects cellular communication by regulating chemical signals and the ion balance among different types of cells.Therefo re,it is not surprising that Na^(+)/K^(+)-ATPase dysfunction has emerged as a risk factor for a variety of neurological diseases.However,published studies have so far only elucidated the important roles of Na^(+)/K^(+)-ATPase dysfunction in disease development,and we are lacking detailed mechanisms to clarify how Na^(+)/K^(+)-ATPase affects cell function.Our recent studies revealed that membrane loss of Na^(+)/K^(+)-ATPase is a key mechanism in many neurological disorders,particularly stroke and Parkinson's disease.Stabilization of plasma membrane Na^(+)/K^(+)-ATPase with an antibody is a novel strategy to treat these diseases.For this reason,Na^(+)/K^(+)-ATPase acts not only as a simple ion pump but also as a sensor/regulator or cytoprotective protein,participating in signal transduction such as neuronal autophagy and apoptosis,and glial cell migration.Thus,the present review attempts to summarize the novel biological functions of Na^(+)/K^(+)-ATPase and Na^(+)/K^(+)-ATPase-related pathogenesis.The potential for novel strategies to treat Na^(+)/K^(+)-ATPase-related brain diseases will also be discussed.
基金Supported by Special Fund for Industrial Technology System Construction of Modern Agriculture (nycytx-024-01-07)Research Special Fund for PublicService Sectors (Agriculture) (200903004)~~
文摘[ Objective] The paper was to understand the biological characteristics of bananamoth [ Opogona sacchari ( Bojer ) ] larvae in sugarcane. [ Method ] Through the rearing test of bananamoth larvae with different hosts, the biological characteristics of bananamoth larvae in sugarcane were observed and analyzed. [ Result ] The mortality rate of bananamoth larvae reared with sugarcane was higher than that of the pests reared with potato. The larva had 8 instars, and the devel- opment duration of the larvae reared with sugarcane was 34.6 d longer than the pests reared with potato. The larvae needed about 98.8 d to complete a generation in average. Viewed from the observation values of head width, body length and weight of larvae, bananamoth larvae reared with sugarcane basically accorded with the growth and development law of larvae. [Condusion ] The results provided theoretical basis for the preparation of effective measures against bananamoth and the assurance of safe production of.sugarcane.
基金Supported by Yunnan Provincial Department of Education,Fund Projects of Young Teachers(06y15SB)Yunnan Science and Technology Project(2006NG16)~~
文摘[Objective] In order to find the pathogenic microorganisms suitable for biological control of filth flies, the pathogenic microorganism was isolated from the dead fly, Boettcherisca peregrine. [Method] The conidia and mycelia were observed by optical microscope. The pathogenic microorganism was identified on the basis of its culture characters and the optical morphologies of the conidia and mycelia, and its biological characteristics and pathogenicity were preliminarily studied. [Result] The pathogenic microorganism isolated from the dead fly was a new strain of Verticillium lecanii. The new strain of V. lecanii was numbered as KMZW-1. The colonies of V. lecanii KMZW-1 grew fastest on potato dextrose agar medium (PDA) at 29 ℃ and pH 6.0. The LC50 of its spore suspension to the adults of B. peregrine, Lucilia sericata, Musca domestic, Piophila casei and Drosophila melanogaster were 9.50×10^5, 4.58×10^7, 4.06×10^7, 4.10×10^3 and 1.05×10^7 conidia/ml, respectively. The LT50 were 6.86, 8.17, 8.16, 8.12 and 3.22 d, respectively. [Conclusion] V. lecanii KMZW-1 is an active pathogenic microorganism to control the adults of five fly species.
基金Supported by National Natural Science Foundation of China(Nos.81271516 and 81371625)Program of Shanghai Science and Technology Commission(Nos.13JC1401503 and 14DZ1930402)+1 种基金Shanghai Municipal Health and Family Planning Commission(No.2013313)Exchange Programme Foundation of Doctoral Student under the Office for Graduate Medical Education,Fudan University
文摘Reliable and non-invasive diagnostic tools are highly valuable for successful therapeutic strategies for the treatment of Alzheimer's disease(AD). The existence of neurofibrillary tangles(NFTs) consisting of tau protein are one kind of the pathological features of AD, and its level of severity is correlated with the stage of AD.However, no clinically approved positron emission tomography(PET) probe is currently available for selective imaging of neurofibrillary tangles on patients. In this paper, we report our studies on biological characteristics of [18F]-THK523 as a novel tau imaging probe. With low molecular weight, [18F]-THK523 is stable, electrically neutral, lipophilic and non-mass concentration-dependent. Preliminary biological studies have shown the excellent properties of [18F]-THK523 as brain imaging tracer for further research.
文摘AIM: To detect the biological characters of the SGC7901 gastric cancer cell-dendritic cell fusion vaccines.METHODS: The suspending living SGC7901 gastric cancer cells and dendritic cells were induced to be fusioned by polyethylene glycol. Pure fusion cells were obtained by selective culture with the HAT/HT culture systems. The fusion cells were counted at different time points of culture and their growth curves were drawn to reflect their proliferative activities. The fusion cells were also cultured in culture medium to investigate whether they could grow into cell clones. MTT method was used to test the stimulating abilities of the fusion cells on T lymphocytes' proliferations. Moreover, the fusion cells were planted into nude mice to observe whether they could grow into new planted tumors in this kind of immunodeficiency animals.RESULTS: The fusion cells had weaker proliferative activity and clone abilities than their parental cells. When they were cultured, the counts of cells did not increase remarkably, nor could they grow into cell clones in culture medium. The fusion cells could not grow into new planted tumors after planted into nude mice. The stimulating abilities of the fusion cells on T lymphocytes' proliferations were remarkably increased than their parental dendritic cells. CONCLUSION: The SGC7901 gastric cancer cell-dendritic cell fusion vaccines have much weaker proliferative abilities than their parental cells, but they keep strong abilities to irritate the T lymphocytes and have no abilities to grow into new planted tumors in immunodeficiency animals. These are the biological basis for their antitumor biotherapies.
基金Supported by State Foreign Experts Bureau Projects(SFEBPS2005#0023)Technology Development Plan Project in Yanbian University(200802)~~
文摘[ Objective ] The study systematically studied the biological characteristics of ginseng Botrytis cinerea Pers. [ Method ] The pure pathogenic fungus was isolated from ginseng B. cinerea collected in the field by tissue segregation and purification cultivation. Subsequently, using PDA medium plate culture method, the effect of various culture conditions on mycelium growth and sporulation of ginseng B. cinerea was detected. [ Result] The optimum temperature for mycelium growth and sporulation of B. cinerea was 25 ℃. The appropriate temperature for conidia germination ranged from 20 to 25 ℃. And the optimum pH value for mycelium growth and conidia germination was 6.0. The optimum carbon source was sucrose, followed by glucose and fructose. The optimum nitrogen source was peptone, fol- lowed by beef extract, yeast extract, alanine and ammonium nitrate. Among the media, the growth of mycelium cultured on PDA medium was the fastest with the production of gray mycelium and dense colonies. Lethal temperatures for sclerotia, mycelium and conidia were 60, 55 and 50 ℃, respectively. [ Conclusion] The study provided the scientific basis for the research on the incidence law of B. cinerea and its control.
基金Supported by Basic and Cutting-edge Technique Research of Henan Province(152300410092)Key Program in Science and Technology of Education Department of Henan Province(15A210020,15B180002,15B180016)~~
文摘The growth characteristics of petroleum-degrader BS-8(Bacillus sp.) and the factors influencing its biosurfactant production were tested; the biosurfactant releasing mode of BS-8 was speculated by measuring OD600, surface tension and oil displacement of fermentation broth; and the effects of carbon source, nitrogen source, temperature, p H, and Na Cl concentration on biosurfactant production by BS-8 were observed in this study. The results showed that the biosurfactant releasing mode of BS-8 was growth-correlated, the surface tension of BS-8 fermentation broth declined with the total bacteria increasing, and the oil displacement was positive correlated with biosurfactant content in fermentation broth; and the optimal culture conditions for effective biosurfactant production included: glucose as carbon source,yeast extract as nitrogen source, Na Cl concentration of 2%, p H of 7.0 and temperature at 30 ℃.
文摘The presented paper is dedicated to a new ret-rospective view on the history of natural sci-ences in XX-XXI cc, partially including the sci-ence philosophy (mainly, the problems of the scientific realism, i.e. the correspondence of science to reality) and also a novel scheme for different classes of sciences with different ob-jects and paradigms. There are analyzed the chosen “great” and “grand” problems of phys-ics (including the comprehension of quantum mechanics, with a recently elaborated new chapter, connected with time as a quantum obs- ervable and time analysis of quantum processes) and also of natural sciences as a whole. The particular attention is paid to the interpretation questions and slightly to the aspects, inevitably connected with the world- views of the res- earchers (which do often constitute a part of the interpretation questions).
基金Key Research and Development Program of Shandong Province(2019GSF107063)Science and Technology Planning Project of Yantai City(2017ZH055).
文摘[Objectives]To study the biological characteristics and culture conditions of Hericium coralloides(Scop.)Pers.[Methods]The wild H.coralloides strain of Changbai Mountain was used as the experimental material to conduct a single factor test on its biological characteristics.[Results]The ideal available carbon source and nitrogen source are glucose and ammonium sulfate,respectively,the pH is 3.5-4.0,and the temperature is 20-22℃.Through the orthogonal experiment of 4 factors and 4 levels,it is found that the degree of influence of the 4 factors on H.coralloides is temperature>pH>nitrogen source>carbon source.[Conclusions]The optimum conditions for the mycelium culture of H.coralloides:the carbon source is glucose,and the nitrogen source is yeast extract or peptone,and pH is 3.5-4.0,and temperature is 20-22℃.
文摘Gypsy moth, Lymantria dispar (L.) is one of the most widespread defoliators of deciduous and larch forests in Kazakhstan. Preferred host plants, flying capacity of females and neonate caterpillars, preferred places for egg laying, major factors of mortality and other characteristics vary considerably between different populations of the pest. According to several year researches on gypsy moth, in Kazakhstan, the main biological characteristics of this pest in the regions were investigated. Results of research showed that gypsy moth exists in central and northern of theses regions. From biological and geographical aspects, gypsy moth which there is in Kazakhstan is close to the same species from Siberia western regions. The pest lays on the different heights of trees trunk. It was found that the number of eggs in each egg mass and egg masses laid by the pest are different. The lack of egg eaters and the over- reproduction of the pest at the natural conditions have resulted in the development of bacterial and viral diseases in the populations of this pest from the regions of Kazakhstan to Siberia west.
基金Supported by the Scientific Research and Technology Development Planning Program of Guangxi Province(10169-08)the Program for the Construction of Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection(Guikeneng1001Z014)~~
文摘[Objective] This study was to investigate the relationship between biological characteristics of Beauveria bassiana (Bals.) Vuill and pathogenicity to Bombyx rnori L, with the aim to provide scientific basis for the control of white muscardine in Bombyx mori L. [Method] The strains isolated and purified from the 6 Beauveria bassiana biocontrol agents from all over the country and the 3 white muscardine silkworms collected from Guangxi provincial silkworm rearing areas were identified by the morphological observation and molecular biology technology. The pathogenicity of B. bassaina to silkworms was determined, and the biological characteristics such as growth diameter, sporulation and the extracellular protease activity of the different B. bassiana strains were compared. [Result] The isolated 9 strains were all B. bassaina (Bals.) Vuillemin, and all strains had high pathogenicity to silkworm, but with different pathogenicities. The growth diameter, sporulation and extracellular protease activity of different B. bassiana strains were also different, and showed correlation with the patheogenicity to silkworms. [Conclusion] B. bassiana spores production amount and exocellular protease activity had significant positive correlation with their pathogenicity to silkworm.