鸡毛菜切段离体培养的研究

分别把鸡毛菜（ＰｔｅｒｏｃｌａｄｉａｔｅｎｕｉｓＯｋａｍ）藻体的顶部、中部、基部切成长２～３ｍｍ的切段，在室温下置培养皿中培养，结果表明，顶部切段的顶端细胞能继续生长，并直接长出新枝；顶部切段的两侧，中部、基部切段的上、下断面及两侧均能长出数量不等的芽体并继而长成新枝，下断面还能长出无色透明的假根丝。光照强度与切段再生的关系密切，在适宜范围内呈正相关，适宜光强为２０００～３０００Ｌｘ，黑暗条件下切段不能成活。

福州夏秋季鸡毛菜品种筛选与综合评价

为加快杂交鸡毛菜新品种的推广应用,丰富福州地区鸡毛菜品种类型,筛选出满足市场需求的鸡毛菜新品种,对引进的9个鸡毛菜品种进行品种比较试验。通过对参试品种农艺学性状、产量、品质及霜霉病抗性的综合评价,结果表明:金品鸡毛菜3号、福青5号和金品青松品种长势强,整齐一致;金品鸡毛菜3号的产量最高,为13235.3 kg·hm^(-2),比对照金品鸡毛菜2号增产40.6%;其次为福青5号和金品青松,分别为12647.1、12352.9 kg·hm^(-2),分别比对照金品鸡毛菜2号增产34.4%和31.2%,金品鸡毛菜3号、福青5号、金品青松与对照相比增产极显著。在霜霉病抗性方面,福青5号、金品青松、金品鸡毛菜4号和金品鸡毛菜3号病情指数分别为5.52、6.84、7.78和8.29,均表现为免疫。综上,筛选出金品鸡毛菜3号、福青5号和金品青松等3个商品性好、产量高、抗性好、综合表现优良的鸡毛菜品种,适宜福州市夏秋季种植。

Influence of bioactive sulphated polysaccharide-protein complexes on hepatocarcinogenesis, angiogenesis and immunomodulatory activities

Objective:To explore the in vivo anticancer,anti-angiogenesis and immunomodulatory efficacies of the bioactive polysaccharide isolated from cold aqueous extract of Jania rubens(JCEM) and Pterocladia capillacea(PCEM) as well as hot aqueous extract of Enteromorpha intestinalis(EHEM) against hepatocellular carcinoma rat model(HCC) and to study their chemical composition.Methods:The sugars and amino acids composition of the bioactive polysaccharides of JCEM,PCEM and EHEM were determined using gas liquid chromatography and amino acid analyzer,respectively.These polysaccharide extracts(20 mg/kg b.wt.for 5 weeks) were assessed on hepatocarcinogenesis in rats and α-fetoprotein(AFP),carcinoembryonic antigen(CEA),glypican-3(GPC-3),hepatocyte growth factor(HGF) and vascular endothelial growth factor(VEGF) and Ig G levels were evaluated.Results:The GLC analysis of JCEM,PCEM and EHEM polysaccharide revealed the presence of 10,9 and10 sugars,in addition the amino acid analyser enable identification of 16,15 and 15 amino acids,respectively.These polysaccharide extracts of JCEM,PCEM and EHEM produced significant decrease in serum AFP,CEA,GPC-3,HGF and VEGF compared with untreated HCC group.JCEM,PCEM and EHEM had an immunostimulatory responses by increasing the IgG levels as compared by naive value(1.23,1.53 and 1.17 folds),respectively.The bioactive polysaccharides in HCC induced rats improved the humoral immune response.The photomicrographs of liver tissue sections of the groups of HCC treated with polysaccharide extracts of Jania rubens and Enteromorpha intestinalis showed intact histological structure.Moreover,fractions HE1,HE4,HE7 obtained from polysaccharide of EHEM showed moderate cytotoxic activity against Hep G2 in vitro with IC_(50) 73.1,42.6,76.2 μg/mL.However,fractions of PCEM and JCEM show no or weak cytotoxicity against Hep G2 in vitro where the cytotoxic activity of their crude polysaccharide extract proved synergetic effect.Conclusions:The pronounced antitumor activity of sulphated polysaccharide-protein complexes of JCEM and EHEM is due to direct cytotoxic activity,anti-hepatocarcinogensis,and anti-angiogenesis.In addition,JCEM,PCEM and EHEM had an immunostimulatory response and improved the humoral immune response in HCC induced rats.

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