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Synergetic Bioproduction of Short-Chain Fatty Acids from Waste Activated Sludge Intensified by the Combined Use of Potassium Ferrate and Biosurfactants
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作者 CHEN Yanyan YAO Shuo +2 位作者 ZHANG Dahai LI Xianguo FENG Lijuan 《Journal of Ocean University of China》 SCIE CAS CSCD 2024年第3期695-709,共15页
The synergetic effect and underlying mechanism of potassium ferrate(PF)with tea saponin(TS,a biosurfactant)in producing short chain fatty acids(SCFAs)from anaerobic fermentation of waste activated sludge(WAS)were expl... The synergetic effect and underlying mechanism of potassium ferrate(PF)with tea saponin(TS,a biosurfactant)in producing short chain fatty acids(SCFAs)from anaerobic fermentation of waste activated sludge(WAS)were explored in this work.Experimental results showed that 0.2 g PF(g TSS)^(-1)(total suspended solid)combined with 0.02 g TS(g TSS)^(-1) could further improve SCFAs’production,and the maximum SCFAs content reached 2008.7 mg COD L^(-1),which is 1.2 and 4.5 times higher than those with PF and TS individually added,respectively,and 5.3 times higher than that of blank WAS on Day 12.In the model substrates experiments,the degradation rates of bovine serum albumin and dextran with combination of PF and TS were 41.3%±0.1% and 48.5%±0.06%,respectively,on Day 3,which are lower than those in blank WAS(with degradation rates of 72.3%±0.5%and 90.3%±0.3%).It was revealed that the oxidative effect of PF and the solubilization of TS caused more organic matters to be dissolved out from WAS,providing a large number of biodegradable substances for subsequent SCFAs production.While WAS pretreated with the combination of PF and TS,the relative abundances of Firmicutes increased from 6.4%(blank)to 38.6%,and that of Proteobacteria decreased from 41.8%(blank)to 21.8%.The combination of PF and TS promoted the hydrolysis process of WAS by enriching Firmicutes,and then increased acetic acid production by inhibiting Proteobacteria that consumed SCFAs.Meanwhile,at the genus level,acidogenesis bacteria(e.g.,Proteiniclasticum and Petrimonas)were enriched whereas SCFAs consuming bacteria(e.g.,Dokdonella)were inhibited. 展开更多
关键词 waste activated sludge anaerobic fermentation potassium ferrate biosurfactant PRETREATMENT
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Antiseptic Efficacy of A Soap Made from Biosurfactants Isolated from Bacillus and Lactobacillus against Pathogenic Bacteria
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作者 Frédéric Yannick Okouakoua Christian Aimé Kayath +10 位作者 Nicaise Saturnin Mokémiabeka Varelle Bervanie Ngala Elenga Digne Nedjea N’goma-Mona Ndelani Nkalla Lambi Sandra Paola Elenga Wilson Christ Dieuveil Bayakissa Malanda Rodinet Tsana Junior Patrick Sergy Bissoko1 Moïse Doria Kaya-Ongoto Duchel Jeanedvi Kinavouidi Etienne Nguimbi 《Advances in Microbiology》 CAS 2024年第1期31-58,共28页
The aim of our study was to use a biosurfactant produced by Bacillus and Lactobacillus isolates as an antiseptic in the formulation of local soap. A total of 60 isolates were characterized by microbiological technique... The aim of our study was to use a biosurfactant produced by Bacillus and Lactobacillus isolates as an antiseptic in the formulation of local soap. A total of 60 isolates were characterized by microbiological techniques (30 Bacillus and 30 Lactobacillus) and the ability to produce biosurfactants was demonstrated by a hydrocarbon emulsification index (E24). The emulsification indexes (E24) varied from 9% to 100% for Bacillus and from 33% to 100% for Lactobacillus as well. The antagonistic assay showed that biosurfactants were able to inhibit the formation of biofilms and growth of pathogens such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Salmonella typhirium, Shigella boydii and Proteus mirabilis. The biosurfactant consortium (BioC) from Bacillus consortium and from Lactobacillus was able to inhibit biofilm formation and the pathogens growth. The BioC was stable to alkaline pH and the temperatures stability of Biosurfactant was ranging from 50°C to 90°C. The soap was made by the cold saponification process using one biosurfactant consortium formulated. This soap has a pH of 10 and showed good cleaning power and good foam stability. Similarly, the soap showed good antiseptic power and disinfection power against all pathogens tested. Handwashing is critical to preventing disease transmission. The persistence of pathogens in waste water was evaluated. The BioS produced showed good disinfection power against all pathogens tested. The valor of reduction on the hands and in the waste water was significantly more than compared to the control soaps used. This soap could be used in the prevention, fighting, and treatment of bacterial and viral infections. 展开更多
关键词 ANTISEPTIC SOAP biosurfactants BACILLUS LACTOBACILLUS DISINFECTION Pathogens bacteria
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Biological Characteristics of Biosurfactantproducing Petroleum-degrader Bacterium Bacillus BS-8
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作者 常慧萍 邢文会 +3 位作者 夏铁骑 付瑞敏 陶令霞 张红 《Agricultural Science & Technology》 CAS 2016年第1期1-3,18,共4页
The growth characteristics of petroleum-degrader BS-8(Bacillus sp.) and the factors influencing its biosurfactant production were tested; the biosurfactant releasing mode of BS-8 was speculated by measuring OD600, s... The growth characteristics of petroleum-degrader BS-8(Bacillus sp.) and the factors influencing its biosurfactant production were tested; the biosurfactant releasing mode of BS-8 was speculated by measuring OD600, surface tension and oil displacement of fermentation broth; and the effects of carbon source, nitrogen source, temperature, p H, and Na Cl concentration on biosurfactant production by BS-8 were observed in this study. The results showed that the biosurfactant releasing mode of BS-8 was growth-correlated, the surface tension of BS-8 fermentation broth declined with the total bacteria increasing, and the oil displacement was positive correlated with biosurfactant content in fermentation broth; and the optimal culture conditions for effective biosurfactant production included: glucose as carbon source,yeast extract as nitrogen source, Na Cl concentration of 2%, p H of 7.0 and temperature at 30 ℃. 展开更多
关键词 biosurfactant Petroleum-degrader BS-8 Growth-correlated Biological characteristic
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Stimulatory effects of biosurfactant produced by Pseudomonas aeruginosa BSZ-07 on rice straw decomposing 被引量:7
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作者 ZHANG Qiuzhuo CAI Weimin WANG Juan 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2008年第8期975-980,共6页
Biosurfactant,produced by Pseudomonas aeruginosa BSZ-07,was added to the dee straw decomposing process to enhance the production of reducing sugars.Observed by Fourier Transform InfraRed(FT-IR)and Nuclear Magnetic Res... Biosurfactant,produced by Pseudomonas aeruginosa BSZ-07,was added to the dee straw decomposing process to enhance the production of reducing sugars.Observed by Fourier Transform InfraRed(FT-IR)and Nuclear Magnetic Resonance(NMR)analysis, the purified biosurfactant was considered as a mixture of RL1 and RL2,which are two different types of rhamnolipids.Two different adding methods,adding the purified rhamnolipid and the on-site production of it were compared.The results showed that 0.5 g/L was the optimum co... 展开更多
关键词 biosurfactant RHAMNOLIPID ON-SITE rice straw decomposing
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Influence of biosurfactant on the diesel oil remediation in soil-water system 被引量:6
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作者 LI Yu-ying ZHENG Xi-lai LI Bing 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2006年第3期587-590,共4页
There were six high diesel oil degrading bacteria strains isolated from the oil contaminated soil that collected from Linzi City. The strain YI was able to produce biosurfactant rhanmolipid when cultivated on diesel o... There were six high diesel oil degrading bacteria strains isolated from the oil contaminated soil that collected from Linzi City. The strain YI was able to produce biosurfactant rhanmolipid when cultivated on diesel oil as carbon source. The critical micelle concentrations (CMC) of rhanmolipid in water and in the soil were measured respectively according to the correlation between the surface tension of the medium and the added rhamnolipid concentration. The results showed that the CMC of rhanmolipid in water was 65 mg/L, and was 185 mg/L in soil. The tests on diesel oil biodegradation were conducted with the addition of different concentrations of rhamnolipid in water and in soil respectively. When 0.01% rhanmolipid was added to water, the diesel oil degradation was enhanced. On the contrary, when the same concentration of rhanmolipid was added to the soil, the degradation of diesel oil was inhibited. The results suggested that the rhamnolipid could enhance the diesel oil biodegradation, indicating that the concentration of rhamnolipid was higher than the corresponding CMC in the medium. Kinetics parameters for the diesel oil biodegradation parameters such as biodegradation constant (λ), coefficient of correlation (r) and half life (t1/2) in both tests were numerically analyzed in this paper, indicating that the moderate concentration of rhamnolipid in the medium could not only enhance the extent of diesel oil biodegradation but also shorten the time for oil remediation. 展开更多
关键词 biosurfactant diesel oil REMEDIATION BACTERIA
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Isolation and characterization of halophilic Bacillus sp.BS3 able to produce pharmacologically important biosurfactants 被引量:5
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作者 MBS Donio SFA Ronica +4 位作者 V Thanga Viji S Velmurugan J Adlin Jenifer M Michaelbabu T Citarasu 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2013年第11期876-883,共8页
Objective:To characterize the pharmacological importance of biosurfactants isolated from halophilic Bacillus sp BS3.Methods:Halophilic Bacillus sp.BS3 was isolated from solar salt works,identified by 16S rRNA sequenci... Objective:To characterize the pharmacological importance of biosurfactants isolated from halophilic Bacillus sp BS3.Methods:Halophilic Bacillus sp.BS3 was isolated from solar salt works,identified by 16S rRNA sequencing and was used for screening their biosurfactant production.Characters of the biosurfactant and their anticancer activity were analyzed and performed in mammary epithelial carcinoma cell at different concentrations.Results:The biosurfactant were characterized by TLC,FTIR and GC-MS analysis and identified as lipopeptide type.GC-MS analysis revealed that,the biosurfactant had various compounds including 13Docosenamide.(Z);Mannosamine,9- and N,N,N',N'-tetramethyl.Surprisingly the antiviral activity was found against shrimp white spot syndrome virus(WSSV) by suppressing the viral replication and significantly raised shrimp survival(P<0.01).Anticancer activity performed in the mammary epithelial carcinoma cell at different concentrations of biosurfactants,among the various concentrations of biosurfactants such as 0.000 25,0.002 5,0.025,0.25 and 2.5 μ g,the 0.25 μ g concentration suppressed the cells significantly(P<0.05) to 24.8%.Conclusions:Based on the findings,the present study concluded that,there is a possibility to develop eco-friendly antimicrobial and anticancer drugs from the extremophilic origin. 展开更多
关键词 HALOPHILIC BACILLUS sp BS3 biosurfactants LIPOPEPTIDE Antimicrobial ANTICANCER
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Effect of biosurfactant on the sorption of phenanthrene onto original and H_2O_2-treated soils 被引量:1
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作者 PEI Xiaohong, ZHAN Xinhua, ZHOU Lixiang College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2009年第10期1378-1385,共8页
The objective of this study was to examine the effect of biosurfactant on the sorption of phenanthrene (PHE) onto the original or HzO2-treated black loamy soil (typic isohumisols) and red sandy soil (typic ferral... The objective of this study was to examine the effect of biosurfactant on the sorption of phenanthrene (PHE) onto the original or HzO2-treated black loamy soil (typic isohumisols) and red sandy soil (typic ferralisols). The sorption isotherms were performed with the original and "soft" carbon-removed soils in the presence and absence of biosurfactant (200 mg/L). The sorption and degradation of biosurfactant were investigated. The result showed that organic matter played an important role in PHE sorption onto the black loamy and red sandy soils, and the PHE sorption isotherms on the "soft" carbon-removed soils exhibited more nonlinearity than those on the original soils. The values of partition coefficient (Kd) on the original black loamy soil with or without 200 mg/L biosurfactant were 181.6 and 494.5 mL/g, respectively. Correspondingly, in the red sandy soil, Kd was 246.4 and 212.8 mL/g in the presence or absence of biosurfactant, respectively. The changes of Kd suggested that biosurfactant inhibited PHE sorption onto the black loamy soil, but facilitated PHE sorption onto the red sandy soil. The nonlinearity of PHE sorption isotherm was decreased in the presence of biosurfactant. Site specific sorption might occur during PHE sorption onto both the original and the "soft" carbon-removed soils in the presence of biosurfactant. It was noted that biosurfactant could also be sorbed onto soils. The maximal sorption capacity of the red sandy soil for biosurfactant was (76.9 ± 0.007) μg/g, which was 1.31 times that of black loamy soil. Biosurfactant was degraded quickly in the two selected soils, and 92% of biosurfactant were mineralized throughout the incubation experiment for 7 d. It implied that biosurfactant should be added frequently when the remediation of polycyclic aromatic hydrocarbon (PAH)-contaminated soils was conducted through PAH desorption approach facilitated by biosurfactant. 展开更多
关键词 biosurfactant PHENANTHRENE SORPTION "soft" carbon soil
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Production and characterization of biosurfactant from Bacillus subtilis CCTCC AB93108 被引量:1
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作者 刘智峰 曾光明 +2 位作者 钟华 傅海燕 刘小兰 《Journal of Central South University》 SCIE EI CAS 2010年第3期516-521,共6页
The production and properties of the biosurfactant synthesized by Bacillus subtilis CCTCC AB93108 were studied. The maximum concentration of the surfactant is 1.64 g/L when the bacteria grow in a medium supplemented w... The production and properties of the biosurfactant synthesized by Bacillus subtilis CCTCC AB93108 were studied. The maximum concentration of the surfactant is 1.64 g/L when the bacteria grow in a medium supplemented with glucose as carbon sources. The isolated biosurfactant is a complex of protein and polysaccharide without lipids. It reduces the surface tension of distilled water to 45.9 mN/m, and its critical micelle concentration (CMC) is 2.96 g/L. It can stabilize emulsions of several aromatic and aliphatic hydrocarbons, such as benzene, xylene, n-pentane, n-nonane, gasoline and diesel oil. It presents high emulsification activity and stability in a wide range of temperature (4-100 ℃) and a long period of duration. 展开更多
关键词 Bacillus subtilis biosurfactant SURFACTANT emulsification activity emulsion stability HYDROPHOBICITY
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Functional characterization of a biosurfactant-producing thermo-tolerant bacteria isolated from an oil reservoir 被引量:1
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作者 Wang Jing Ji Guang +3 位作者 Tian Jing Zhang Hongdan Dong Hanping Yu Li 《Petroleum Science》 SCIE CAS CSCD 2011年第3期353-356,共4页
A Bacillus subtilis strain JA-1 isolated from an oil reservoir was studied. This strain is capable of growth and producing biosurfactant at a temperature of 60 ℃. In nutrient medium it produced biosurfactant which re... A Bacillus subtilis strain JA-1 isolated from an oil reservoir was studied. This strain is capable of growth and producing biosurfactant at a temperature of 60 ℃. In nutrient medium it produced biosurfactant which reduced the surface tension from 68.2 mN/m to 28.3 mN/m, with the critical micelle concentration (CMC) of 48 mg/L. The measured surface tension indicated that the biosurfactant possessed stable surface activity at high temperature and a specific range of pH and salt concentrations. The results of thin layer chromatography (TLC) together with FT-IR showed that the metabolic product of strain JA-1 is a lipopeptide biosurfactant. The ability to growth at high temperature and to produce biosurfactant makes strain JA-1 promising for enhanced oil recovery. 展开更多
关键词 biosurfactant bacillus subtilis LIPOPEPTIDE surface tension critical micelle concentration(CMC)
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Optimization of Biosurfactant Production from Glycerol by Pseudomonas Aeruginosa EQ 109 Using Factorial Design 2^3 被引量:1
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作者 Francisca Pessoa De Franca Flavia Padilha Bastos Dos Santos Lea Maria de Almeida Lopes 《Journal of Chemistry and Chemical Engineering》 2010年第10期1-12,共12页
One possible application for the excess of glycerol, which is an exceeding byproduct in biodiesel industry, was used as carbon and energy sources for bioproducts synthesis. This work aims to evaluate biosurfactant pro... One possible application for the excess of glycerol, which is an exceeding byproduct in biodiesel industry, was used as carbon and energy sources for bioproducts synthesis. This work aims to evaluate biosurfactant production from glycerol by Pseudomonas aeruginosa EQ 109 isolated from crude oil-contaminated soil. Factorial design 2^3 was utilized to optimize the amount of biosurfactant produced, by using pH (A), initial biomass concentration (B), and initial glycerol concentration (C) as independent factors. The experiments were carried out in flasks containing 100 mL of mineral medium. Biosurfactant production was monitored by increase of the emulsification of aviation kerosene (E24) and surface tension reduction (STr). The results have shown that, at pH = 7.0, in order to increase E24, variables as B and C are the most influential, leading to a maximum value of E24 = 79%, as well as for an increase of GC (GCmax = 49%). STR was the variable with the best correlation factor for the proposed linear model (R2=0.96) and its maximum value was 48%. Xfwas not significant to the model, although it was influenced by pH and C, with C = 40g/L (Xfmax = 4.56 g/L). 展开更多
关键词 BIODIESEL biosurfactant factorial design GLYCEROL Pseudomonas aeruginosa.
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Optimized Biosurfactant Production by <i>Pseudomonas aeruginosa</i>Strain CGA1 Using Agro-Industrial Waste as Sole Carbon Source 被引量:1
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作者 Chikodili G. Anaukwu Chioma M. Ogbukagu Ikechukwu A. Ekwealor 《Advances in Microbiology》 2020年第10期543-562,共20页
Biosurfactants are biomolecules produced by microorganisms, which possess several advantages over their chemical counterparts. Production can be cost-effective if renewable wastes are utilized as substrates. In this s... Biosurfactants are biomolecules produced by microorganisms, which possess several advantages over their chemical counterparts. Production can be cost-effective if renewable wastes are utilized as substrates. In this study, optimization of biosurfactant production by <i>Pseudomonas aeruginosa</i> strain CGA1 was carried out using response surface methodology. The conventional “One factor at a time” method of optimization was initially adopted to ascertain the impact of different renewable wastes on biosurfactant production. Four independent variables were tested: carbon and nitrogen concentration, medium volume, and inoculum size. Biosurfactant production was based on the emulsification index measurement. Results indicated that the preferred carbon source by the isolate was sugar cane molasses. A 2.31-fold increase in biosurfactant yield and emulsification index of 96.3% ± 0.75% under optimized cultural conditions of 20 g/L of molasses, 5 g/L of sodium nitrate, 1.93 ml inoculum size and 60 ml medium volume in 250 ml conical flask were obtained. The regression coefficient (R<sup>2</sup>) value of 84.15% implied adequate fitness of the model. The surface tension of distilled water was reduced from 72.1 mN/m to 35.0 ± 0.0 mN/m, and critical micelle concentration was attained at 60 mg·L<sup>-1</sup>. FTIR and GC-MS analysis indicated that the biosurfactant was a lipopeptide having characteristic lipid and peptide peak values. This study proves that the sole use of agro-industrial wastes for the production of biosurfactant is very efficient, and ensures the economic feasibility of biosurfactant production. 展开更多
关键词 Pseudomonas aeruginosa Optimization Response Surface Methodology biosurfactant Sugar Cane Molasses LIPOPEPTIDE
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Biosurfactant-producing Ability of Highly Efficient Petroleum-degrading Bacterium BS-8
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作者 Huiping CHANG Tieqi XIA +2 位作者 Hongpeng HAN Ruimin FU Hong ZHANG 《Agricultural Biotechnology》 CAS 2015年第4期61-63,69,共4页
After incubation for 6 -30 h, with the rapid increase of bacterial cell number, surface tension of bacterium BS-8 was reduced sharply from 63.2 mN/m to 39.4 mN/m. The production of biosurfactants by BS-8 was growth-de... After incubation for 6 -30 h, with the rapid increase of bacterial cell number, surface tension of bacterium BS-8 was reduced sharply from 63.2 mN/m to 39.4 mN/m. The production of biosurfactants by BS-8 was growth-dependent. Using glucose as the carbon source, bacterium BS-8 was incubated. Based on centrifugation, precipitation and chromogenie reaction of the culture solution, results indicated that the biosurfactants belonged to lipopeptides. The yield of biosurfaetants isolated and purified from the culture solution was 0.58 g/L, and the critical micelle concentration (CMC) was 90 mg,/L. Under conditions of pH 4 -9, tem- perature 20 -70 ℃, NaCl concentration 1% -6%, biosurfactants predueed by BS-8 exhibited the highest stability. 展开更多
关键词 PETROLEUM Degrading bacterium BS-8 biosurfactants LIPOPEPTIDES Stability
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Biosurfactant Production by Pseudomonas aeruginosa and Yarrowia lipolytica and Its Use for Detergent Formulations
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作者 Silvanito Alves Barbosa Diego de Freitas Coelho +2 位作者 Edgar Silveira Elias Basile Tambourgi Roberto Rodrigues de Souza 《Journal of Chemistry and Chemical Engineering》 2013年第8期767-773,共7页
This work reports detergents production using biological surfactants, microbiologically synthesized, and compares its foaming power and emulsification capacity to those presented by a petroleum based surfactant. Both ... This work reports detergents production using biological surfactants, microbiologically synthesized, and compares its foaming power and emulsification capacity to those presented by a petroleum based surfactant. Both used microorganisms were capable to produce surfactants, been able to emulsify oil/water mixtures and cause decrease of surface tension of water. The biosurfactant produced from Yarrowia lipolytica has a critical micelle concentration lower than that obtained from Pseudomonas aeruginosa (10 and 30 mg·Lt, respectively), but the later showed better results in foaming power and emulsification experiments, similar to the synthetic detergent. 展开更多
关键词 Biodetergent biosurfactant Pseudomonas aeruginosa Yarrowia lipolytica.
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Current Research and Strategy on the Biosurfactants Used in the Remediation of Petrochemical Polluted Environment
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作者 Cheng Shuiming Liu Jiefeng +2 位作者 Lu Yan Tan Liquan Zhou Tian 《Meteorological and Environmental Research》 CAS 2014年第2期59-62,共4页
Biosurfactants are biologically active metabolites, and the efficiency of direct screening of new biosurfactants from nature using traditional methods is low, which should be enhanced in the following studies by adopt... Biosurfactants are biologically active metabolites, and the efficiency of direct screening of new biosurfactants from nature using traditional methods is low, which should be enhanced in the following studies by adopting advanced biotechnologies. Rapid development and wide application of microbial culture independent methods, such as metagenomics, metatranscriptomics, metaproteomics and metabonomics, etc., contributes to quickly and precisely screening of novel biological surfactants. We mainly represented the current status of research and applications of biosurfactants in the remediation of petrochemical polluted environment, and also prospected avenues for future research. 展开更多
关键词 biosurfactants Petrochemical pollution Environmental remediation Uncultured microbial technology China
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Optimization Techniques and Development of Neural Models Applied in Biosurfactant Production by <i>Bacillus subtilis</i>Using Alternative Substrates
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作者 Juliana Ferrari Ferreira Secato Brunno Ferreira dos Santos +1 位作者 Alexandre Nunes Ponezi Elias Basile Tambourgi 《Advances in Bioscience and Biotechnology》 2017年第10期343-360,共18页
Bacillus subtilis was investigated as production of biosurfactant using a combination based on waste of candy industry and glycerol from biodiesel production process as only substrate. The experimental design chosen f... Bacillus subtilis was investigated as production of biosurfactant using a combination based on waste of candy industry and glycerol from biodiesel production process as only substrate. The experimental design chosen for optimization by response surface methodology was a central composite rotatable design (CCRD) and dry weight (DW) and crude biosurfactant (CB) concentrations were selected as responses in analysis. Two techniques were implemented response surface methodology (RSM) and artificial neural network (ANN). First challenge of study was to assess the effects of the interactions between variables and reach optimum values. With the CCRD results, RSM and ANN models were developed, optimizing the production of biosurfactant. The correlation coefficients (R2) of RSM models explained 88% for DW and 73% for CB of the interactions among substrate concentrations, while ANN models explained 99% for DW and 98% for CB, demonstrating that developed ANN models were more accurate and consistent in predicting optimized conditions than RSM model. The maximum DW and CB produced in the optimum conditions were 25.60 ± 5.0 g/L and 668 ± 40 mg/L, respectively. The crude biosurfactant also showed applications in cases of oil spreading in water due to clear zone produced in Petri dishes assays. 展开更多
关键词 biosurfactant BACILLUS SUBTILIS Response Surface Methodology Artificial Neural Network Oil Spreading Waste Management
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Mineralization of Petroleum Contaminated Wastewater by Co-Culture of Petroleum-Degrading Bacterial Community and Biosurfactant-Producing Bacterium
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作者 Bo young Jeon Il Lae Jung Doo Hyun Park 《Journal of Environmental Protection》 2011年第7期895-902,共8页
Activity of a crude biosurfactant extracted from the culture fluid of Serratia sp. that was isolated from riverbed soil was shown to increase in proportion to the cultivation time, and was higher at pH 8 than at pH 7.... Activity of a crude biosurfactant extracted from the culture fluid of Serratia sp. that was isolated from riverbed soil was shown to increase in proportion to the cultivation time, and was higher at pH 8 than at pH 7. Serratia sp. grew in the mineral-based medium with soybean oil but was not with kerosene-diesel. The petroleum-degrading bacteria—Acinetobacter sp., Pseudomonas sp., Paracoccus sp., and Cupriavidus sp.—were isolated from a specially designed enrichment culture. The efficiency of mineralization of wastewater contaminated with kerosene and diesel (WKD) by the petroleum-degrading bacterial community (PDBC) was enhanced significantly by addition of the crude biosurfactant. The efficiency of mineralization of the WKD was also about 2 times boosted by co-culture of Serratia sp. and PDBC. Bacterial community of Serratia sp. and PDBC co-cultivated in the WKD was maintained for at least 8 days according to the TGGE pattern of 16S rDNA obtained from the bacterial culture. In conclusion, the co-culture of Serratia sp. and PDBC is an applicable technique for the mineralization of wastewater contaminated with petroleum, which may substitute for chemical or biological surfactant. 展开更多
关键词 biosurfactant SERRATIA Sp. Petroleum-Degrading Bacteria Mixed Culture TGGE
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Electrokinetic and Flotation Investigations of Surface Properties Modification of Magnesite and Serpentinite Using Biosurfactants and Surfactants
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作者 Agnieszka Didyk-Mucha Izabela Polowczyk +1 位作者 Zygmunt Sadowski Jan Kudelko 《Journal of Physical Science and Application》 2015年第2期87-95,共9页
This paper presents some results of zeta potential measurements performed into biosurfactant adsorption onto magnesite and serpentinite surface. Zeta potential and isoelectric point measurement of magnesite and serpen... This paper presents some results of zeta potential measurements performed into biosurfactant adsorption onto magnesite and serpentinite surface. Zeta potential and isoelectric point measurement of magnesite and serpentinite particles before and after interaction with biosurfactant broth solution and activator (nickel(II) ion solution) were carried out. The zeta potential results show that presence of biosurfactants changes both magnesite and serpentinite surface potential by physical adsorption which increases the hydrophobicity of mineral particles. Measurements of particles zeta potential in the presence of biosurfactant broth are relevant to the minerals flotation. Hallimod flotation response of magnesite and serpentinite as a function of collector concentration was investigated. Bioflotation test results show that at the presence of broth, the flotation separation of magnesite from serpentinite is possible. 展开更多
关键词 Zeta potential adsorption BIOFLOTATION biosurfactant activation SERPENTINITE magnesite.
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Optimization and Evaluation of Biosurfactant Produced by Pantoea sp. Using Pineapple Peel Residue, Vegetable Fat and Corn Steep Liquor
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作者 Fabiola Carolina Gomes de Almeida Thayse Alves de Lima e Silva +3 位作者 Ian Garrard Leonie Asfora Sarubbo Galba Maria de Campos-Takaki Elias Basile Tambourgi 《Journal of Chemistry and Chemical Engineering》 2015年第4期269-279,共11页
In this study, the authors have investigated the potential of a bacterial strain of Pantoea sp., isolated from wastewater of the textile industry, for the production of biosurfactant. The biosurfactant production was ... In this study, the authors have investigated the potential of a bacterial strain of Pantoea sp., isolated from wastewater of the textile industry, for the production of biosurfactant. The biosurfactant production was optimized by the combination of CCD (central composite design) and RSM (response surface methodology). To assess the effects and interactions of medium the vegetable fat (1.5, 2.0 and 2.5 v/v), the variables corn steep liquor (2.0, 5.0 and 8.0 v/v) and pineapple peel residue (10.0, 25.0 and 40.0 v/v) on the surface tension were evaluated. The empirical model developed through RSM in terms of the effective operational factors mentioned above was found to be adequate to describe the biosurfactant production. Compositional analysis of the produced biosurfaetant has been carried out by FT-IR (Fourier transform infrared spectoscopy) and subjected to the test of removing hydrocarbons. Through the analysis, vegetable fat and pineapple peel residue were found to be the most significant factors, whereas corn steep liquor had less effect within the ranges investigated. A maximum reduction in surface tension of 30.00 mN/m was obtained under the optimal conditions of 2.0% (v/v) vegetable fat concentration, 5.0% (v/v) corn steep liquor and 25.0% (v/v) pineapple peel residue concentration of medium. FT-IR spectrometer analysis of the biosurfactant characterized it as a glycolipid derivative. The biosurfactant exhibited the ability to solubilize the hydrocarbons tested, working between 64% and 92%. According to consists of bars with a length proportional to the absolute value of the estimated effects divided by the standard error. On this chart, ANOVA (analysis of variance) effect estimates are arranged from the largest to smallest absolute value. The chart includes a vertical line at the critical p-value of 0.05. Effects for which the bars are smaller than the critical p-value are considered non-significant and do not have an effect on the response variables. The effects are either positive or negative ANOVA; the determination of regression coefficients and the construction of graphs were performed using the Statistical program, version 7.0 (Statsoft Inc, RSA). The results, the biosurfactant produced by Pantoea sp. can be a valuable source for application in rapid environmental bioremediation. 展开更多
关键词 biosurfactant OPTIMIZATION bioremediation.
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Application of Biosurfactant from Candida sphaerica UCP 0995 in Removal of Petroleum Derivative from Soil and Sea Water
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作者 Humberto Bezerra de Souza Sobrinho Juliana Moura de Luna +2 位作者 Raquel Dinz Rufino Ana Lucia Figueiredo Porto Leonie Asfora Sarubbo 《Journal of Life Sciences》 2013年第6期559-569,共11页
The biosurfactant produced by Candida sphaerica in a fermentor containing 5% vegetal oil refinery waste and 2.5% was tested in the removal of motor oil from soils and seawater. In kinetic assays, the isolated biosurfa... The biosurfactant produced by Candida sphaerica in a fermentor containing 5% vegetal oil refinery waste and 2.5% was tested in the removal of motor oil from soils and seawater. In kinetic assays, the isolated biosurfactant removed more than 86% of the motor oil adsorbed to clay, silty and sandy soils at the critical micelle concentration (CMC). Static removal tests performed in glass columns demonstrated that the crude biosurfactant was able to remove 75% and 92% of the oil contained in clay and silty soil, respectively, whereas the isolated biosurfactant at its CMC removed 50% of the oil from sandy soil. In the washing of hydrophobic compound on a porous surface, the removal rate was 60%. The biosurfactant also proved to be efficient in detergency tests since the crude surfactant removed 41% of motor oil from contaminated cotton cloth. In tests carried out with seawater, the crude biosurfactant showed an oil spreading efficiency of 75% in both screening dispersion test and oil displacement efficiency methods. Regarding the swirling bottle test, the dispersion rate was 72% for the isolated biosurfactant at a concentration twice the CMC. The biosurfactant studied demonstrated potential for application as an adjuvant in biotechnological processes for environmental decontamination. 展开更多
关键词 Candida sphaerica biosurfactant motor oil bioremediation.
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Giving More Benefits to Biosurfactants Secreted by Lactic Acid Bacteria Isolated from Plantain Wine by Using Multiplex PCR Identification
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作者 Michaelle Bokamba Moukala Christian Aimé Kayath +4 位作者 Gabriel Ahombo Nicole Prisca Makaya Dangui Duchel Jeanedvi Kinouani Kinavouidi Effort Concentré Nguimbi Mouélé Martin Diatewa 《Advances in Microbiology》 2019年第11期917-930,共14页
Fermented beverages have continued to give more surprises in terms of the presence of biomolecules and the diversity of microorganisms that may be contained. Republic of Congo is home to a panoply of fermented foods a... Fermented beverages have continued to give more surprises in terms of the presence of biomolecules and the diversity of microorganisms that may be contained. Republic of Congo is home to a panoply of fermented foods and beverages that are still not yet studied. This is the case of plantain wine fluently called banana wine. Within this framework, this work aims to study the role of Biosurfactant-like Biomolecules secreted during fermentation of plantain wine. Using MRS medium, 15 isolates bacteria have been found. 100% are able to secrete biosurfactant and 66.66% are extractible biosurfactants. 33% of isolates have been associated to Lactobacillus plantarum (Is2, Is9, Is12 and Is13) by using a one-step multi-plex PCR that targets genes encoding for bacteriocins. Biosurfactants secreted by L. plantarum play an important role in the preservation of banana wine. The biosurfactants extracted with chloroform and ammonium sulphate are able to inhibit the growth of pathogenic bacteria including Shigella flexneri, Salmonella spp., Pseudomonas aeruginosa and Staphy-lococcus aureus. 展开更多
关键词 PLANTAIN WINE FERMENTED BEVERAGE LACTOBACILLUS spp. Multiplexe PCR biosurfactants Pathogenic Inhibition
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