从南楚文化发展链中看《文子》对屈原思想的影响

本文分析了作为上源文化的《文子》对屈原思想发生作用的可能性，并对《文子》思想与屈原思想构成素质进行了比较，从而看出同处南楚文化发展链中的《文子》对屈原思想的影响之深刻。

《文子》重生思想简论

文子》的重生思想是在物欲极度膨胀的社会背景下提出来的 ,其内容包括贵身贱物、形气神兼修等思想 ,其重生的目的不仅仅是为了保养身体 ,从形而上的角度说重生以复归“道”、“德”为最终目的 ,从形而下的角度讲 ,重生又是治理天下的基础。这不但是老子、《文子》等学说的特点 。

Study on Cloning and Polymorphism of Porcine Adiponectin Promoter

[ Objective] The aim of this study was to provide a basis for study on adiponectin as a candidate gene for fat deposition. [ Method] The promoter sequence of adiponectin was obtained by porcine BAC library screening and primer-walking method. The polymorphisms of adiponectin promoter from 290 pigs, including 5 breeds of Lantang pig, Large spotted pig, Large white pig, Landrace and Duroc, were analyzed with PCR-RFLP. [ Result] At SNP site of adiponectin 5＇-flanking region -1 010 bp （G/A）, GG genotype frequency in Chinese indigenous pigs was significantly higher than that in exotic pigs. At SNP site of adiponectin 5＇-flanking region -394 bp （T/C）, the genotype distribution of Chi- nese indigenous pigs was abundant, while no CC genotype was detected in exotic pigs, and T allele frequency was higher in exotic pigs. [ Conclusion] SNP site mutation of - 1 010 bp （G/A） may lead to changes of the gene transcription level, while SNP site of -394 bp （T/C） properly has no relationship with gene transcription level and fat deposition.

Construction and Quality Analysis of Full-length cDNA Library of Phyllostachys heterocycla Germinating Seeds

[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to construct the full-length cDNA library by using Oligo-capping method. [Result] The constructed library has a total capacity of 6.5×10^6 recombinant clones, and a low proportion of clones without inserted frag- ments; the size of inserted fragments ranges between 0.3-5.0 kb, with strict classifi- cation and ideal consistency. Furthermore, the proportion of clones harboring long in- serted fragments （1.0-5.0 kb） is as high as 30%, achieving the standard for high- quality full-length cDNA library. [Conclusion] The full-length cDNA library of germinat- ing seeds of P. heterocycla was successfully constructed, which laid important foun- dation for the functional genomics research of bamboo plants.

Isolation and Characterization of Microsatellites in Snap Bean

The objectives of this study were to isolate and characterize microsatellites from a heat tolerant variety of snap bean (Phaseolus vulgaris L.) in order to generate polymorphic genetic markers linked to quantitative trait loci for heat tolerance. A genomic library contained 400-800 bp inserts was constructed and screened for the presence of (GA/CT) n and (CA/GT) n repeats. The proportion of positive clones yielded estimated of 3.72×10 4 such dinucleotide repeats per genome, roughly comparable to the abundance reported in other eukaryotic genomes. Twenty_six positive clones were sequenced. In contrast to mammalian genomes, the (GA/CT) n motif was much more abundant than the (CA/GT) n motif in these clones. The (GA/CT) n repeats also showed longer average repeat length (mean n =10.4 versus 6.5), suggesting that they are better candidates for yielding polymorphic genetic markers in the snap bean genome.

郑樵的汉字生成理论

本文根据《六书略》等有关材料，对郑樵的汉字生成理论作了一次探索，分析了“书画同出”、“起一成文”、“子母相生”等学说的内涵，并探讨了其汉字生成理论的内在逻辑关系，认为他的汉字生成理论已形成一个自成体系的框架，具有独创性，但也存在粗疏、幼稚之弊。

《汉书·艺文志》“杂家”蠡测

《汉书·艺文志·诸子略》有“九流十家”,杂家为其中“一家一流”。班固言杂家者流盖出于王官之议官,而周官议官后世无从可考,且汉以后历代志书杂家类书目变动极大,使后世学者对于杂家究竟为何种学派争论不休。襍与雑虽俱为今简体“杂”字之繁体,但二字本意尚有所区别,战汉时期所言杂家当写作襍家更为恰当。通过将战汉史料与《汉志》《隋志》对襍家定义对比研究后可发现,襍家与西汉早期之前的博士官特征极为相似,为襍家学者或即当时之博士。汉以后博士为儒者垄断,又武帝“罢黜百家”后儒家以外百家学皆为杂学,而诸子又皆有他名固称无需再易其名,原“博士家”也就是后来的襍家,再称博士之名已不再合宜,故刘、班以“襍家”名之。

施蛰存与鲁迅的交往新说

施蛰存和鲁迅同为我国现代文学史上影响广泛深远的小说家、散文家、诗人、翻译家和编辑家,又同样为学贯中西、博古通今而又深受广大青年爱戴的一代名教授和严谨学者,他们之间的关系自上世纪30年代迄今,成为中国现代文学史上众所瞩目的话题,乃至颇有学术价值的研究课题。文章详尽揭示有关施、鲁之争的“《庄子》与《文选》”这近百年的文坛公案,并全面论述了施蛰存与鲁迅之间多年互相支持与合作的友好交往。

Archaeal and bacterial communities in acid mine drainage from metal-rich abandoned tailing ponds, Tongling, China

To expand knowledge on microbial communities of various metal-rich levels of mine drainage environments in Anhui province, China, the archaeal and bacterial diversities were examined using a PCR-based cloning approach. Eight acid mine water samples were collected from five areas in Tongling. Phylogenetic analyses revealed that bacteria mainly fell into ten divisions, which were Betaproteobacteria, Gammaproteobacteria, Alphaproteobacteria, Deinococcus-Thermus, Nitrospira, Firmicutes, Actinobacteria, Deltaproteobacteria, Bacteroidetes, Chloroflexi. Archaea fell into three phylogenetic divisions, Thermoplasma, Ferroplasma and Thermogymnomonas. The unweighted pair group method with arithmetic mean（UPGMA） cluster analysis based on the microbial communities’ compositions revealed that five samples shared similarity with the dominance of Meiothermus and Thermomonas. Two samples had the preponderant existence of Acidithiobacillus and Leptospirillum. The remaining sample owned higher microbial communities’ diversity with the Shannon-Weaver H up to 2.91. Canonical correlation analysis（CCA） suggested that microbial community structures had great association with p H and the concentration of Hg2＋, Pb2＋, Fe3＋, Cl-, SO2- 4in water.

Developmental Expression of an Amphioxus (Branchiostoma belcheri) Gene Encoding a GATA Transcription Factor

GATA factors are evolutionarily conserved and play crucial roles during embryonic development in both vertebrates and invertebrates. Vertebrate GATAs can be divided into two subgroups, the GATAI/2/3 and the GATA4/5/6 classes. Through genomic analysis, we have identified three GATA factors, representing the GATA1/2/3 and GATA4/5/6 subfamilies respectively, and one GATA like protein in the genome of the basal chordate amphioxus （Branchiostoma floridae, cephalochordata）. Partial sequence of GATA123 in the amphioxus Branchiostoma belcheri （BbGATA123） was cloned and its expression pattern during early embryonic development was studied. Expression of BbGATA123 is first detected in the mesendoderm during gastrulation. Interestingly, in the late neurula and early larva stages, it is expressed strongly in the cerebral vesicle and the mid gut region. Its expression is compared to Otx, a gene known crucial for the development of anterior structures. Our observations suggest that GATA123, together with Otx, might play an important role in the development of amphioxus cerebral vesicle, the counterpart of the vertebrate brain.

The Case for Presupposition： On Kripke＇s Anaphoric Account

According to Kripke, traditional approaches to presupposition and anaphora do not work in some compound sentences because traditionally, they have been thought to assign presuppositions to each clause in isolation. I agree with this criticism, but also think that context is required in order to determine the presupposition included in a complex sentence. To show the context role in fixing the utterance truth-conditions in those cases, this paper introduces the Kripkean anaphoric account on presuppositions （section 2）, and then criticizes this approach because it is very restrictive （section 3）. The paper concludes that to solve those difficulties, one should appeal to a two-dimensional framework, including a new parameter that focuses on the sentence according to the concrete features of the context： the speaker＇s point of view （section 4）.

Analysis of English literary works based on face theory

Brown ＆ Levinson＇s face theory is an important theory in Pragmatics and it is an influential theory in expounding the phenomenon of politeness. Through an analysis of the functions of ＂face＂ in English literary works, this article intends to shed new lights on literary pragmatics.

The Global Value ofMencius＇s Ideas on Moral Feeling and Reason： Reinterpreting the Feeling of Compassion From the Perspective of the Philosophy of Emotion

Amid the recent global upsurge of studies on the philosophy of emotion, a reinterpretation of traditional Confucian philosophy, especially Mencius＇s ideas on the siduan （Four Original Sources） of morality, may shed new light on the subject in comparative philosophy. This paper probes the structure of moral feeling and reason described by Mencius＇s from five perspectives： （1） In view of the relationship between feeling and reason, is it better to use the expression ＂siduan＂ or the expression ＂sixin （Four Heart-minds）＂？ （2） In view of dispositional feeling, what are the four original sources？ （3） In view of moral feeling, what are the structural order of the four sources and the corresponding procedure of reasoning of four heart-minds of Humanity？ （4） In view of positive feeling, how does moral feeling grow out of the goodness of human nature？ （5） What is the global value of Mencius＇s ideas on human moral feeling？ The author concludes that Mencius＇s thought on moral feeing has a global value and cross-cultural significance, and that Chinese wisdom is more than regional but universally applicable. The structure of moral feeling and reason that Mencius identified is in accordance with the principles ofzhiliang （grasping the two poles of the beginning and the end） and yongzhong （emloying the middle）. The principles ofzhiliang and yongzhong are true universal wisdoms of Confucianism, which should be rekindled today. From a practical point of view, ＂the Way begins from moral feelings,＂ and Confucius and Yah Hui＇s seeking the simple, virtuous life is an ideal model of emotional well-being.

Molecular identification and culture observation on Acrochaete leptochaete (Chaetophoraceae, Chlorophyta) from China

Acrochaete leptochaete （Huber） Nielsen （Chaetophoraceae, Chlorophyta） was isolated from the macroalgae Chaetomorpha collected from intertidal pools in Rongcheng, Shandong, China. 18S rDNA combined with ITS regions were used to ascertain the morphological identification of the isolated material. Based on the unialgal culture, asexual reproduction and growth characteristics of A. leptochaete were investigated over wide ranges of temperature and irradiance. Results revealed that asexual reproduction of A. leptochaete could be realized by biflagellate zoospores. The zoospores germinated directly to give self- replicating generations. Zoospore germination was bipolar. A temperature range from 13-21 ℃ and a lower irradiance of 36 μmol/（m2.s） were most favorable for the growth ofA. leptochaete. Thallus organization, an important taxonomic criterion for the genus Acrochaete, was affected markedly by temperature and irradiance. Our results extend the knowledge about the species＇ general biology and its morphological plasticity. For classification and identification of a simple microphytic algae like A. leptochaete, which are traditionally placed in the class Chaetophoraceae, we propose that molecular tools associated with culture observations are applied.

The Symbolism of the Skull in Vanitas： Homo Bulla Est

In Dutch vanitas imagery of Homo bulla est there is a sad child （homo） holding a scalloped shell filled with soap and water. Although the child is amused and playing with the formation of beautiful transparent water circles, his expression is mischievous and melancholic. Blooming flowers and dead trees, burning urns, and cloudy landscapes also accompany this gloomy imagery, which alludes to a warning about moral behavior. The first part of this essay deals with a brief history about the symbolism of the skull in vanitas imagery （1590-1630）. And the second part of the essay focuses on two prints by the Dutch artist and printmaker from Haarlem, Hendrick Goltzius （1558-1617）, who honored this proverb Homo bulla est in Allegory of Transience as a remembrance of the brevity of life.

Molecular identification and culture trials of Eutreptiella gymnastica (Eutreptiales, Euglenophyceae)

During a survey for marine microalgal resources, we isolated a rare marine euglenoid from the coastal waters of Qingdao, China in 2009, and established a pure culture. Electron microscopic and molecular phylogenetic （18S rDNA and 16S rDNA sequences） analyses revealed a close affinity with Eutreptiella gymnastica, a bloom-forming species. Different culture conditions were monitored to understand optimal E. gymnastica growth characteristics. The optimal growth conditions in a batch culture of this isolate were 20C, 160 gmol photons/（mE.s） of white light, and a salinity of 10-31. Nutrient experiments demonstrated that growth increased dramatically with a phosphorus concentration greater than 72 gmol/L. Understanding the effect of culture conditions on E. gymnastica may help understanding the blooming mechanism of this alga in its natural environment.

Capital Budgeting： Methods, Aspects, and Issues

This paper furthers the understanding of capital budgeting （CB） processes. It emphasizes the assumed disposition of the forecast cash benefits from projects. The two reasons for CB are discussed. First, which projects are and are not acceptable from a financial prospective？ Second, of financially acceptable projects, CB methods can rank or order projects relative to desirability. Under conditions of certainty, the paper describes and contrasts three primary methods： pay back （PB）, net present value （NPV）, and internal rate of return （IRR） and three secondary methods： profitability index （PI）, terminal value （TV）, and modified internal rate of return （MIRR）. The presentation lists the strengths and weaknesses of each method in order to direct attention to some of the many aspects of CB decisions that firm managers should consider. Next, approaches for taking risk into consideration are discussed. The discussion raises the important issues of what to do with the net cash benefits from various projects when and if they are received. Finally, the paper provides flexible tools and example problems for teaching CB in a variety of contexts, such as professional corporate training or academic coursework.

Methods Comparison for Microsatellite Marker Development:Different Isolation Methods,Different Yield Efficiency

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology,quantitative genetics and genomics. Therefore,it is extremely necessary to select several versatile,low-cost,efficient and time-and labor-saving methods to develop a large panel of microsatellite markers. In this study,we used Zhikong scallop(Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency,while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time-and cost-saving,it is difficult to obtain a large number of microsatellite markers,mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study,we recommend two methods,microsatellite-enriched library construction method and FIASCO-colony hybridization method,for large-scale microsatellite marker development. Both methods were derived from the microsatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.

Effects of Land-Cover Changes and Other Remediations on Hydrology of Xinjiang River Sub-Watershed

To determine whether reforestation efforts in the denuded hills have significant impacts on hydrology in the Xinjiang River watershed, the authors examined eight land-cover scenarios to compare hydrologic responses and to provide a conceptual basis for restoration practices. The authors analyzed a 17-year time period using remote sensing to develop land-cover classification for the watershed. Climate, soil and terrain data for the watershed were used as input in the SWAT （soil and water analysis tool） to quantify and compare the impacts on hydrologic processes. The model was calibrated to a two-year record of stream discharge measurements. The results show significant increase in forest-cover on hills （13%）. However, the hydrological response is not very significant considering the changes in forest-cover, the surface runoff and percolation ratios only changed by 2% and 1% over time. Installment of earthen irrigation ponds in the outlets of sub-basin with maximum runoff had provided the most significant hydrologic improvements and could provide irrigation water to increase crop yield on remaining cropland. The study will provide information to the local government to aid decision-making in sustainable reforestation programs resulting in better hydrologic functioning for sustainable water resource management.

Modified molecular matrix model for predicting molecular composition of naphtha＇

To improve the naphtha composition prediction model based on molecular type homologous series matrix （MTHS）, this paper puts forward a novel molecular matrix to characterize the naphtha composition and the norreal distribution hypothesis to better describe the molecular composition distribution within each homologous series of the molecular matrix. Through prediction calculation of eight groups of naphtha samples and eight groups of gasoline samples, it is verified that the normal distribution hypothesis is more applicable than gamma distribution hypothesis for the prediction model. According to the prediction results of the samples, the restrain range of normal distribution parameters during model computing process is summarized. With the bulk properties of naphtha samples and the value range of distribution parameters as input conditions, this study utilizes the improved novel molecular matrix to predict the composition of naphtha samples. As the results show, the novel molecular matrix can predict more detailed composition information of naphtha and improve prediction accuracy with less unknown parameters.