This study aimed to achieve rapid detection of Pseudomonas syringae pv.tabaci,the pathogen of tobacco wildfire disease.The specific primers and probes for recombinase-aided amplification(RAA)were designed with HrpZ as...This study aimed to achieve rapid detection of Pseudomonas syringae pv.tabaci,the pathogen of tobacco wildfire disease.The specific primers and probes for recombinase-aided amplification(RAA)were designed with HrpZ as the target gene.RAA was then combined with the lateral flow dipstick(LFD)to establish a LFD-RAA-based rapid detection system for the pathogen.Furthermore,the detection performance of the established method was tested.The results showed that the LFDRAA method had high specificity.The amplification could be completed after 25 min of reaction at 39℃.The sensitivity of the established method reached 0.0001 ng/μL,which was superior to that of PCR detection.Moreover,the LFD-RAA method could quickly detect P.syringae pv.tabaci from tobacco leaves,demonstrating field applicability.To sum up,the LFD-RAA method established in this study can be applied in the rapid detection and early diagnosis of tobacco wildfire disease.展开更多
[Objective] This study aimed to preliminarily determine the physiological mechanism of glyphosate resistance produced in wild soybean(Glycine soja) and further provide a basis for the breeding of glyphosate-resistan...[Objective] This study aimed to preliminarily determine the physiological mechanism of glyphosate resistance produced in wild soybean(Glycine soja) and further provide a basis for the breeding of glyphosate-resistant soybean. [Method] First,a screening for glyphosate resistant varieties among sixty-seven wild soybean materials was done in a field trial; subsequently, physiological indexes of the screened resistant variety ZYD0685 and the sensitive variety ZYD0790 were studied. [Result]At the glyphosate dose of 1.23 kg a.i/hm2, glyphoaste resistance varied greatly among different wild soybean materials, with the highest survival rate of 87% and83% occurring in ZYD0685 and ZYD2405, respectively, and that of another seven accessions ranged from 2.7% to 38%, and all the remaining fifty-eight soybean materials died. After treatment with glyphoaste at different doses, there were no significant differences in chlorophyll content and shikimate content in the resistant ZYD0685, but there was an evident increase in the activity of gultathione-S-transferases(GSTs); while in the sensitive ZYD0790, the content of shikimic acid increased significantly, and chlorophyll content decreased significantly, and GSTs activity revealed a slight change. [Conclusion] Therefore, lowering the amount of accumulated shikimic acid is the major physiological response to glyphosate in wild soybean.展开更多
Two species of the genus Mutuuraia Munroe from China are reported in this paper. Among them, M. flavimacularis sp. nov., is described as new to science with male genital structure illustrated.
Bamboo,is very important for its value and rapid growth,is mostly cultivated in China.Pyraustinae are severely injurious to bamboo.The external morphology of the last-instar larvae of Algedonis coclesalis(Walker),Cr...Bamboo,is very important for its value and rapid growth,is mostly cultivated in China.Pyraustinae are severely injurious to bamboo.The external morphology of the last-instar larvae of Algedonis coclesalis(Walker),Crocidophora evenoralis Walker and Demoboly pervulglis Hampson(all Pyraustini injurious to bamboo) are described and illustrated.Biological information for these larvae is provided.展开更多
[Objective] This study aimed to investigate the mutagenic effect of 60 Co γ-rays irradiation on turf characteristics of Buchloe dactyloides (Nutt.) Engelm. [Method] Buffalo grass were irradiated with 60 Co γ-rays ...[Objective] This study aimed to investigate the mutagenic effect of 60 Co γ-rays irradiation on turf characteristics of Buchloe dactyloides (Nutt.) Engelm. [Method] Buffalo grass were irradiated with 60 Co γ-rays at five different radiation intensities (1 200, 1 400, 1 600, 1 800, 2 000 Gy) to determine the related turf characteristics and analyze the mutagenic effect of 60 Co γ-rays at different radiation intensities on buffalo grass. [Result] Germination rates of buffalo grass irradiated by different radiation intensities of 60 Co γ-rays varied inconsistently, and the root length and bud length were shorter than the control; compared with the control, the height of irradiated seedlings was significantly reduced, and the number of tillers, plant height, leaf length and leaf blade width were smaller than the control; however, the stolon length, stolon diameter and number of stolon nods had no significant difference compared with the control. [Conclusion] This study laid foundation for determining the appropriate radiation intensity of 60 Co γ-rays and selecting useful mutants of buffalo grass.展开更多
Two species of the genus Epiparbattia Caradja from China are reported in this paper. One new species, Epiparbattia oligotricha sp. Nov., is described with genital structures illustrated.
Urochondra setulosa (Trin.) C.E. Hubbard is a coastal halophytic grass thriving on the coastal dunes along the Pakistani seashore. This grass could be useful in coastal sand dune stabilization using seawater irrigat...Urochondra setulosa (Trin.) C.E. Hubbard is a coastal halophytic grass thriving on the coastal dunes along the Pakistani seashore. This grass could be useful in coastal sand dune stabilization using seawater irrigation. The purpose of this investigation was to test the hypothesis that Ca^2+ (0.0, 2.5, 5.0, 10.0 and 50.0 mmol/L) alleviates the adverse effects of KC1, MgSO4, NaC1 and Na2SO4 at 0, 200, 400, 600, 800 and 1000 mmol/L on the germination of Urochondra setulosa. Seed germination was inhibited with increase in salt concentration with few seeds germinated at and above 400 mmol/L concentration. No seed germinated in any of the KC1 treatments. Inclusion of CaC12 substantially alleviated the inhibitory effects of all salts. Germination was higher under photoperiod in comparison to those seeds germinated under complete darkness. Among the CaC12 concentrations used, 10 mmol/L was most effective in alleviating salinity effects and allowing few seeds to germinate at 1000 mmol/L KC1, MgSO4, NaC1 and Na2SO4 solution.展开更多
[Objective] The study aimed to explore the degradation law and trend of artificial grassland. [Method] Taking the ryegrass (Lolium perenne) - white clover ( Trifolium repens) artificial grassland in Maiping Townsh...[Objective] The study aimed to explore the degradation law and trend of artificial grassland. [Method] Taking the ryegrass (Lolium perenne) - white clover ( Trifolium repens) artificial grassland in Maiping Township, Guizhou Province as the research object, the grassland vegetation of 40 quadrate from different areas (area around the sheep shed, hilltop, hillside, flatland at the foot of the hill) were analyzed by comparing the dominance and richness index. [ Result] Degradation of different degrees appeared in various areas of this artificial grassland. To be specific, the degradation of grassland around the sheep shed was not serious for less consumption by the sheep; however, the areas on the hilltop and hillside degenerated to the grassland types of dallis grass ( Paspalum dilatatum Poir. ) -Indian lovegrass ( Eragrostis pilosa) and dallis grass - cogongrass [ Imperata cylindrica ( Linn. ) Beauv. ], respectively, and the area at the foot of the hill degenerated to the grassland type dominated by garland chrysanthetnum ( Chrysanthemum coronarium L. ) and knotgrass ( Paspalum distichum L. ). [ Conclusion ] This study provided a basis for grassland improvement as well as the efficient and sustainable utilization of grazing-type artificial grassland in South China.展开更多
Land animals as well as all organisms in ocean synthesize sulfated polysaccharides. Fungi split from animals about 1.5 billion years ago. As fungi make the evolutionary journey from ocean to land, the biggest changes ...Land animals as well as all organisms in ocean synthesize sulfated polysaccharides. Fungi split from animals about 1.5 billion years ago. As fungi make the evolutionary journey from ocean to land, the biggest changes in their living environment may be a sharp decrease in salt concentration. It is established that sulfated polysaccharides interact with hundreds of signaling molecules and facilitate many signaling transduction pathways, including fibroblast growth factor (FGF) and FGF receptor signaling pathway. The disappearance of sulfated polysaccharides in fimgi and plants on land might indicate that polysaccharides without sulfation might be sufficient in facilitating protein ligand/receptor interactions in low salinity land. Recently, it was reported that plants on land start to synthesize sulfated polysaccharides in high salt environment, suggesting that fungi might be able to do the same when ex- posed in such environment. Interestingly, Cordyceps, a fungus habituating inside caterpillar body, is the most valued traditional Chi- nese Medicine. One of the important pharmaceutical active ingredients in Cordyceps is polysaccharides. Therefore, we hypothesize that the salty environment inside caterpillar body might allow the fungi to synthesize sulfated polysaccharides. To test the hypothesis, we isolated polysaccharides from both lava and sporophore of wild Cordyceps and also from Cordyceps militaris cultured without or with added salts. We then measured the polysaccharide activity using a FGF2/FGFRlc signaling-dependent BaF3 cell proliferation assay and found that polysaccharides isolated from wild Cordyceps activated FGF2/FGFR signaling, indicating that the polysaccha- rides synthesized by wild Cordyceps are indeed different from those by the cultured mycelium.展开更多
[ Objective] This study was to explore the inheritance of BC1 F3 to excellent characteristics of parents( Elymus dahuricus, Hordeum brevisubu/atum), heterosis and fertility restoration. E Methodl The agronomic chara...[ Objective] This study was to explore the inheritance of BC1 F3 to excellent characteristics of parents( Elymus dahuricus, Hordeum brevisubu/atum), heterosis and fertility restoration. E Methodl The agronomic characters such as growth period, growth rate, pollen fertility, fecundity, reproducibility and fresh grass output of Elymus dahudcus, Hordeum brevisubu/atum and BC1 F3 were comparatively studied. E Result l The growth dynamic of the BC1 F3 lined tended to like that of its parent Hordeum brevisubu/atum; although growing periods of various lines were different, they were close to that of Hordeurn brevisubulatum. There were differences in pollen fertility and seed setting, the pollen fertility rate of YF3-93 was higher than recurrent parent Hordeum brevisubulatum, its seed setting rate in open pollination was also higher. Some lines had low pollen fertility rate and seed setting rate, such as the pollen fertility rate of PF3-52. There were significant differences in the output among BC1 F3 lines, e.g. the heterosis (HP) of YF3-64, YF3-74 and YF3-83 were 75.53%, 75.12% and 66.16%, respectively; however, the yields of PF3- 52, PF3-15 and PF3-42 were lower than their parents. ~ Conclusion] This study provided reference for breeding new varieties of forage which is suitable to drought and saline environment.展开更多
Genetic diversity study of wild Philippine paddy straw mushroom (Volvariella volvacea) was conducted to establish a germplasm collection accessible to volvariella producers in the Philippines. Forty one wild strains...Genetic diversity study of wild Philippine paddy straw mushroom (Volvariella volvacea) was conducted to establish a germplasm collection accessible to volvariella producers in the Philippines. Forty one wild strains were collected from different geographical areas in Northern and Central Luzon region. Strains were differentiated using random amplified polymorphic DNA (RAPD). A single 10-based primer was used to generate randomly amplified polymorphic DNA (RAPD) in V. volvacea and differences were noted in band size (bp) ranging from 1,800 bp to 550 bp. Principal component analysis (PCA) of the RAPD data revealed 4 groups from wild strains. One strain showed RAPD pattern with band appearance at 1,750, 950 and 750 bp; 3 strains at 1,800 and 750 bp; 8 strains at 1,500 and 550; and the most abundant group with 29 strains at 750 bp. With observed lack of heterogeneity among strains, it is recommended that more collections from the wild should be undertaken for more diverse germplasm collection. Moreover, it is suggested that RAPD can be used to delineate strains of V. volvacea with potential importance on genetic diversity conservation and breeding.展开更多
Field observation was conducted at typical rangeland in Mongolia to examine the frontal area index. Frontal area index is the characteristics of surface roughness elements affecting sand transport. The frontal area wa...Field observation was conducted at typical rangeland in Mongolia to examine the frontal area index. Frontal area index is the characteristics of surface roughness elements affecting sand transport. The frontal area was strongly correlated with the effective shelter length on sand transport in both short grasses and shrubs. The frontal area index was 0.031 and the relationship between the roughness length and frontal area index in this site agreed with past studies.展开更多
This study was conducted using seeds in Petri dish containing agar medium in order to determine acetyl-CoA carboxylase (ACCase) herbicides resistance (R) in Avena sterilis that was grown in wheat fields at Adana p...This study was conducted using seeds in Petri dish containing agar medium in order to determine acetyl-CoA carboxylase (ACCase) herbicides resistance (R) in Avena sterilis that was grown in wheat fields at Adana province, Turkey. Seeds were collected from one large suspected field, where clodinafop-propargyl (Aryloxyphenoxypropionate “FOPs”) and pinoxaden (Phenylpyrazoline “DEN”) have been applied for many years. Susceptible (S) population was collected from the road side on the same region. Agar media of concentration 14 g/L was prepared and it was melted in microwave. Then the amount of 20 mL agar media was added into each Petri dish. Five seeds were placed on agar mediums containing discriminating dose of clodinafop and pinoxaden. Petri dishes were placed in growth incubator operating at 10 °C. After 15 d, both radicle and hypocotyl length were measured. The percentage of germinated seed and dose-response curves were determined. At these different concentration levels, there were more than 50% of R and less than 40% of S seed germinated for pinoxaden. However, for clodinafop, more than 60% of R and less than 50% of S seeds were germinated. At higher concentration levels, the populations of resistant and susceptible were not germinated for both herbicides. The resistance value of R population was then compared with that of the S biotype. From the resistance index (RI), the population was more resistant to pinoxaden (7.43 for radicle and 2.47 for hypocotyl) than the clodinafop-propagyl (1.39 for radicle and 3.77 for hypocotyl). The method provided a simple, quick and cost effective way to identify ACCase herbicides resistance in most grass weeds.展开更多
文摘This study aimed to achieve rapid detection of Pseudomonas syringae pv.tabaci,the pathogen of tobacco wildfire disease.The specific primers and probes for recombinase-aided amplification(RAA)were designed with HrpZ as the target gene.RAA was then combined with the lateral flow dipstick(LFD)to establish a LFD-RAA-based rapid detection system for the pathogen.Furthermore,the detection performance of the established method was tested.The results showed that the LFDRAA method had high specificity.The amplification could be completed after 25 min of reaction at 39℃.The sensitivity of the established method reached 0.0001 ng/μL,which was superior to that of PCR detection.Moreover,the LFD-RAA method could quickly detect P.syringae pv.tabaci from tobacco leaves,demonstrating field applicability.To sum up,the LFD-RAA method established in this study can be applied in the rapid detection and early diagnosis of tobacco wildfire disease.
基金Supported by the National Natural Science Foundation of China(30971834)~~
文摘[Objective] This study aimed to preliminarily determine the physiological mechanism of glyphosate resistance produced in wild soybean(Glycine soja) and further provide a basis for the breeding of glyphosate-resistant soybean. [Method] First,a screening for glyphosate resistant varieties among sixty-seven wild soybean materials was done in a field trial; subsequently, physiological indexes of the screened resistant variety ZYD0685 and the sensitive variety ZYD0790 were studied. [Result]At the glyphosate dose of 1.23 kg a.i/hm2, glyphoaste resistance varied greatly among different wild soybean materials, with the highest survival rate of 87% and83% occurring in ZYD0685 and ZYD2405, respectively, and that of another seven accessions ranged from 2.7% to 38%, and all the remaining fifty-eight soybean materials died. After treatment with glyphoaste at different doses, there were no significant differences in chlorophyll content and shikimate content in the resistant ZYD0685, but there was an evident increase in the activity of gultathione-S-transferases(GSTs); while in the sensitive ZYD0790, the content of shikimic acid increased significantly, and chlorophyll content decreased significantly, and GSTs activity revealed a slight change. [Conclusion] Therefore, lowering the amount of accumulated shikimic acid is the major physiological response to glyphosate in wild soybean.
基金The research was supported by the National Natural Science Foundation of Chinafor the special program( 1 0 8)
文摘Two species of the genus Mutuuraia Munroe from China are reported in this paper. Among them, M. flavimacularis sp. nov., is described as new to science with male genital structure illustrated.
基金supported by the Zhejiang Natural Science Foundation (Y3080105)
文摘Bamboo,is very important for its value and rapid growth,is mostly cultivated in China.Pyraustinae are severely injurious to bamboo.The external morphology of the last-instar larvae of Algedonis coclesalis(Walker),Crocidophora evenoralis Walker and Demoboly pervulglis Hampson(all Pyraustini injurious to bamboo) are described and illustrated.Biological information for these larvae is provided.
基金Supported by National Science and Technology Support Project of China (2009BADA7B04, 2011BAD17B01-02)National 973 Project of China (2012CB215300)+1 种基金National 863 Project of China (2012AA101201)National Pasture Modern Industry Technology System of China (CARS-35)~~
文摘[Objective] This study aimed to investigate the mutagenic effect of 60 Co γ-rays irradiation on turf characteristics of Buchloe dactyloides (Nutt.) Engelm. [Method] Buffalo grass were irradiated with 60 Co γ-rays at five different radiation intensities (1 200, 1 400, 1 600, 1 800, 2 000 Gy) to determine the related turf characteristics and analyze the mutagenic effect of 60 Co γ-rays at different radiation intensities on buffalo grass. [Result] Germination rates of buffalo grass irradiated by different radiation intensities of 60 Co γ-rays varied inconsistently, and the root length and bud length were shorter than the control; compared with the control, the height of irradiated seedlings was significantly reduced, and the number of tillers, plant height, leaf length and leaf blade width were smaller than the control; however, the stolon length, stolon diameter and number of stolon nods had no significant difference compared with the control. [Conclusion] This study laid foundation for determining the appropriate radiation intensity of 60 Co γ-rays and selecting useful mutants of buffalo grass.
基金The research was supported by the Post-Doctoral Science Foundation of China(2004035035) and the Natural Science Foundation of Guangdong (04300537)
文摘Two species of the genus Epiparbattia Caradja from China are reported in this paper. One new species, Epiparbattia oligotricha sp. Nov., is described with genital structures illustrated.
基金Project supported by the 16th Pakistan-China Cooperation Project(Study on Sustainable Halophytes Utilization, No. 16-413)
文摘Urochondra setulosa (Trin.) C.E. Hubbard is a coastal halophytic grass thriving on the coastal dunes along the Pakistani seashore. This grass could be useful in coastal sand dune stabilization using seawater irrigation. The purpose of this investigation was to test the hypothesis that Ca^2+ (0.0, 2.5, 5.0, 10.0 and 50.0 mmol/L) alleviates the adverse effects of KC1, MgSO4, NaC1 and Na2SO4 at 0, 200, 400, 600, 800 and 1000 mmol/L on the germination of Urochondra setulosa. Seed germination was inhibited with increase in salt concentration with few seeds germinated at and above 400 mmol/L concentration. No seed germinated in any of the KC1 treatments. Inclusion of CaC12 substantially alleviated the inhibitory effects of all salts. Germination was higher under photoperiod in comparison to those seeds germinated under complete darkness. Among the CaC12 concentrations used, 10 mmol/L was most effective in alleviating salinity effects and allowing few seeds to germinate at 1000 mmol/L KC1, MgSO4, NaC1 and Na2SO4 solution.
基金Supported by National Key Technology R & D Program(2006BAD16B07) Fund of Science and Technology in Guizhou Province ([2008]2074)~~
文摘[Objective] The study aimed to explore the degradation law and trend of artificial grassland. [Method] Taking the ryegrass (Lolium perenne) - white clover ( Trifolium repens) artificial grassland in Maiping Township, Guizhou Province as the research object, the grassland vegetation of 40 quadrate from different areas (area around the sheep shed, hilltop, hillside, flatland at the foot of the hill) were analyzed by comparing the dominance and richness index. [ Result] Degradation of different degrees appeared in various areas of this artificial grassland. To be specific, the degradation of grassland around the sheep shed was not serious for less consumption by the sheep; however, the areas on the hilltop and hillside degenerated to the grassland types of dallis grass ( Paspalum dilatatum Poir. ) -Indian lovegrass ( Eragrostis pilosa) and dallis grass - cogongrass [ Imperata cylindrica ( Linn. ) Beauv. ], respectively, and the area at the foot of the hill degenerated to the grassland type dominated by garland chrysanthetnum ( Chrysanthemum coronarium L. ) and knotgrass ( Paspalum distichum L. ). [ Conclusion ] This study provided a basis for grassland improvement as well as the efficient and sustainable utilization of grazing-type artificial grassland in South China.
基金supported by the National Natural Science Foundation of China (Grant No. 91129706)
文摘Land animals as well as all organisms in ocean synthesize sulfated polysaccharides. Fungi split from animals about 1.5 billion years ago. As fungi make the evolutionary journey from ocean to land, the biggest changes in their living environment may be a sharp decrease in salt concentration. It is established that sulfated polysaccharides interact with hundreds of signaling molecules and facilitate many signaling transduction pathways, including fibroblast growth factor (FGF) and FGF receptor signaling pathway. The disappearance of sulfated polysaccharides in fimgi and plants on land might indicate that polysaccharides without sulfation might be sufficient in facilitating protein ligand/receptor interactions in low salinity land. Recently, it was reported that plants on land start to synthesize sulfated polysaccharides in high salt environment, suggesting that fungi might be able to do the same when ex- posed in such environment. Interestingly, Cordyceps, a fungus habituating inside caterpillar body, is the most valued traditional Chi- nese Medicine. One of the important pharmaceutical active ingredients in Cordyceps is polysaccharides. Therefore, we hypothesize that the salty environment inside caterpillar body might allow the fungi to synthesize sulfated polysaccharides. To test the hypothesis, we isolated polysaccharides from both lava and sporophore of wild Cordyceps and also from Cordyceps militaris cultured without or with added salts. We then measured the polysaccharide activity using a FGF2/FGFRlc signaling-dependent BaF3 cell proliferation assay and found that polysaccharides isolated from wild Cordyceps activated FGF2/FGFR signaling, indicating that the polysaccha- rides synthesized by wild Cordyceps are indeed different from those by the cultured mycelium.
文摘[ Objective] This study was to explore the inheritance of BC1 F3 to excellent characteristics of parents( Elymus dahuricus, Hordeum brevisubu/atum), heterosis and fertility restoration. E Methodl The agronomic characters such as growth period, growth rate, pollen fertility, fecundity, reproducibility and fresh grass output of Elymus dahudcus, Hordeum brevisubu/atum and BC1 F3 were comparatively studied. E Result l The growth dynamic of the BC1 F3 lined tended to like that of its parent Hordeum brevisubu/atum; although growing periods of various lines were different, they were close to that of Hordeurn brevisubulatum. There were differences in pollen fertility and seed setting, the pollen fertility rate of YF3-93 was higher than recurrent parent Hordeum brevisubulatum, its seed setting rate in open pollination was also higher. Some lines had low pollen fertility rate and seed setting rate, such as the pollen fertility rate of PF3-52. There were significant differences in the output among BC1 F3 lines, e.g. the heterosis (HP) of YF3-64, YF3-74 and YF3-83 were 75.53%, 75.12% and 66.16%, respectively; however, the yields of PF3- 52, PF3-15 and PF3-42 were lower than their parents. ~ Conclusion] This study provided reference for breeding new varieties of forage which is suitable to drought and saline environment.
文摘Genetic diversity study of wild Philippine paddy straw mushroom (Volvariella volvacea) was conducted to establish a germplasm collection accessible to volvariella producers in the Philippines. Forty one wild strains were collected from different geographical areas in Northern and Central Luzon region. Strains were differentiated using random amplified polymorphic DNA (RAPD). A single 10-based primer was used to generate randomly amplified polymorphic DNA (RAPD) in V. volvacea and differences were noted in band size (bp) ranging from 1,800 bp to 550 bp. Principal component analysis (PCA) of the RAPD data revealed 4 groups from wild strains. One strain showed RAPD pattern with band appearance at 1,750, 950 and 750 bp; 3 strains at 1,800 and 750 bp; 8 strains at 1,500 and 550; and the most abundant group with 29 strains at 750 bp. With observed lack of heterogeneity among strains, it is recommended that more collections from the wild should be undertaken for more diverse germplasm collection. Moreover, it is suggested that RAPD can be used to delineate strains of V. volvacea with potential importance on genetic diversity conservation and breeding.
文摘Field observation was conducted at typical rangeland in Mongolia to examine the frontal area index. Frontal area index is the characteristics of surface roughness elements affecting sand transport. The frontal area was strongly correlated with the effective shelter length on sand transport in both short grasses and shrubs. The frontal area index was 0.031 and the relationship between the roughness length and frontal area index in this site agreed with past studies.
文摘This study was conducted using seeds in Petri dish containing agar medium in order to determine acetyl-CoA carboxylase (ACCase) herbicides resistance (R) in Avena sterilis that was grown in wheat fields at Adana province, Turkey. Seeds were collected from one large suspected field, where clodinafop-propargyl (Aryloxyphenoxypropionate “FOPs”) and pinoxaden (Phenylpyrazoline “DEN”) have been applied for many years. Susceptible (S) population was collected from the road side on the same region. Agar media of concentration 14 g/L was prepared and it was melted in microwave. Then the amount of 20 mL agar media was added into each Petri dish. Five seeds were placed on agar mediums containing discriminating dose of clodinafop and pinoxaden. Petri dishes were placed in growth incubator operating at 10 °C. After 15 d, both radicle and hypocotyl length were measured. The percentage of germinated seed and dose-response curves were determined. At these different concentration levels, there were more than 50% of R and less than 40% of S seed germinated for pinoxaden. However, for clodinafop, more than 60% of R and less than 50% of S seeds were germinated. At higher concentration levels, the populations of resistant and susceptible were not germinated for both herbicides. The resistance value of R population was then compared with that of the S biotype. From the resistance index (RI), the population was more resistant to pinoxaden (7.43 for radicle and 2.47 for hypocotyl) than the clodinafop-propagyl (1.39 for radicle and 3.77 for hypocotyl). The method provided a simple, quick and cost effective way to identify ACCase herbicides resistance in most grass weeds.