The classification of infrasound events has considerable importance in improving the capability to identify the types of natural disasters.The traditional infrasound classification mainly relies on machine learning al...The classification of infrasound events has considerable importance in improving the capability to identify the types of natural disasters.The traditional infrasound classification mainly relies on machine learning algorithms after artificial feature extraction.However,guaranteeing the effectiveness of the extracted features is difficult.The current trend focuses on using a convolution neural network to automatically extract features for classification.This method can be used to extract signal spatial features automatically through a convolution kernel;however,infrasound signals contain not only spatial information but also temporal information when used as a time series.These extracted temporal features are also crucial.If only a convolution neural network is used,then the time dependence of the infrasound sequence will be missed.Using long short-term memory networks can compensate for the missing time-series features but induces spatial feature information loss of the infrasound signal.A multiscale squeeze excitation–convolution neural network–bidirectional long short-term memory network infrasound event classification fusion model is proposed in this study to address these problems.This model automatically extracted temporal and spatial features,adaptively selected features,and also realized the fusion of the two types of features.Experimental results showed that the classification accuracy of the model was more than 98%,thus verifying the effectiveness and superiority of the proposed model.展开更多
[Objective] The aim was to explore the growth characteristics of different quantity leaflet compound leaves of Juglans cathayensis in Xinjiang so as to provide basic data for the study of J.cathayensis.[Method] The le...[Objective] The aim was to explore the growth characteristics of different quantity leaflet compound leaves of Juglans cathayensis in Xinjiang so as to provide basic data for the study of J.cathayensis.[Method] The length,width and biomass of compound leaves growth characteristics of three types of different quantity leaflet compound leaves of J.cathayensis were measured in large samples.[Result] The length,width,rachis biomass and total biomass of compound leaves increased with the number of leaflets increase;leaflets biomass of the same phyllotaxy decreased with the number of leaflets increase.The leaflet length,width and biomass of the same compound leaves increased with the number of phyllotaxy increase.The differences on petiole length and top leaflets length were not significant;there were significant differences on compound leaves width;the differences on the total biomass of compound leaves were extremely significant.[Conclusion] There was rather large ecology plasticity of the compound leaves of J.cathayensis in Xinjiang.展开更多
Glyptosternoid fishes are a group of sisorid catfishes living in torrents of rivers mainly originating from the Qinghai-Tibet Plateau. Based on our survey in the Drung River Basin, seven collecting sites were investig...Glyptosternoid fishes are a group of sisorid catfishes living in torrents of rivers mainly originating from the Qinghai-Tibet Plateau. Based on our survey in the Drung River Basin, seven collecting sites were investigated and 271 glyptosternoid fishes caught belong to three species (Pareuchiloglanis kamengensis, Exostoma labiatum and Oreoglanis mocropterus). Features of the distribution of the three catfishes were assessed. More individuals of E. labiatum were caught in the lower reaches of the Drung River with fast water velocity and it might be more adapted to a torrent habitat. The relationships between standard length (L) and weight (W) for P. kamengensis, E. labiatum and O. macropterus were also studied, and the parameter b of the L-W relationship (W = aL^b) ranged between 2. 8201 and 3. 0131. From the present study, all the three catfish species grow allometrically and the growth type of E. labiatum is the closest to a symmetrical one.展开更多
Based on the clay minerals, content analysis from 187 stations in the surface sediments, this paper dealt with the assemblages and the distributions of clay minerals in the surface sediments off the Yangtze River estu...Based on the clay minerals, content analysis from 187 stations in the surface sediments, this paper dealt with the assemblages and the distributions of clay minerals in the surface sediments off the Yangtze River estuary. The research results showed that the illite dominates the surface sediments off the Yangtze River estuary with the smectite or kaolinite the second rank and chlorite the third rank; the illite-smectite-kaolinite-chlorite-assemblage is the main type of the clay minerals assemblage in the area, with the illite-kaolinite-chlorite-smectite-type as the second rank; the source of clay mineral is mainly from the Yellow River and the Yangtze River. The Q-type cluster analysis show that sediments from both recent and ancient Yellow River were deposited mainly in the northeast part of the study area to the north of 29°30′. Substance from the Yangtze River was deposited mainly in the western and middle parts off the Yangtze River estuary.展开更多
The fiber length trait (FLT) of 538 individuals from nature birch population in Maorshan region, Heilongjang, China were measured, of which 100 individuals were selected as representative variety of correlated fragm...The fiber length trait (FLT) of 538 individuals from nature birch population in Maorshan region, Heilongjang, China were measured, of which 100 individuals were selected as representative variety of correlated fragments screening with random amplified polymorphism DNA (RAPD) technique. In total of 20 RAPD primers were tested through multiple regression analysis between amplified strip and the character behaviors, and a correlative segment BFLR-16 was obtained. The correlation coefficient between BFLI-16 and FLT was 0.6144, with the significant level of 1%. BFLI-16 was then cloned, sequenced and transformed into SCAR marker. The percentage of identifying long fiber birches by this SCAR was more than 92. The result indicates that the SCAR markers has high specificity for the long fiber individuals and is highly linked with the gene controlling the character of fiber length, and its existence is significantly correlative with the increase in the fiber length.展开更多
SiC nanowires were prepared on C/C composite surface without catalyst by chemical vapor deposition(CVD) using CH3 SiCl3 as precursor.SEM images of the CVD-product reveal that some long nanowires have grown to tens o...SiC nanowires were prepared on C/C composite surface without catalyst by chemical vapor deposition(CVD) using CH3 SiCl3 as precursor.SEM images of the CVD-product reveal that some long nanowires have grown to tens of micrometers with some gathered as a ball.Some short nanowires agglomerate like chestnut shell with many thorns accompanied by some deposited nano-particles.XRD,Raman-spectrum and FTIR patterns indicate that the product is a typical β-SiC.TEM images show that the nanowires have a wide diameter range from 10 to 100 nm,and some thin nanowires are bonded to the thick one by amorphous CVD-SiC.A SiC branch generates from an amorphous section of a thick one with an angle of 70° between them,which is consistent with the [111] axis stacking angle of the crystal.SAED and fast Fourier transform(FFT) patterns reveal that the nanowires can grow along with different axes,and the bamboo-nodes section is full of stacking faults and twin crystal.The twisted SiC lattice planes reveal that the screw dislocation growth is the main mechanism for the CVD-SiC nanowires.展开更多
The research analyzed birthplace, work site and Work unit, discipline, agri- cultural product variety, current age, and selected age, as well as internal and exter- nal factors for growth, which providers references f...The research analyzed birthplace, work site and Work unit, discipline, agri- cultural product variety, current age, and selected age, as well as internal and exter- nal factors for growth, which providers references for exploring development of high- level agricultural S&T talents and formulation of relevant policies.展开更多
The growth and development of Jingjiang Taro [Colocasia esculenta(L.) Schott] were observed, laying foundation for highly-yielding cultivation technology system of Xiangsha taro. The results indicate that germinatio...The growth and development of Jingjiang Taro [Colocasia esculenta(L.) Schott] were observed, laying foundation for highly-yielding cultivation technology system of Xiangsha taro. The results indicate that germination of the second taro happened from 46 d after sprouting (7-8 leaves); the first taro began to expand, from 125 d after sprouting (12 leaves) when the the second taro was been formed; the third taro began to form from 141 to 150 d after sprouting (15-16 leaves). The fresh weight of the second and third taros quickly increased from the time of 15 leaves to harvest. Early September is a transformation term of Xiangsha taro from vegetative body dominant to corm growth dominant.展开更多
The plant growth and physiological and biochemical responses of root and leaves of grape Yinhong seedlings to the weak lights of 20 000,16 000,12 000,8 000 lx and the normal illumination of 25 000 lx(CK) respectivel...The plant growth and physiological and biochemical responses of root and leaves of grape Yinhong seedlings to the weak lights of 20 000,16 000,12 000,8 000 lx and the normal illumination of 25 000 lx(CK) respectively,were investigated.There was no significant dfference in growth indexes of root and leaves of grapevine seedlings between the light of 20 000 lx and CK,and the light of 16 000 lx and CK for 30 d.The chlorophyll contents,soluble protein contents,net photosynthetic rates,transpiration rates,stomatal conductance,water use efficiency and protective enzyme(CAT,POD,SOD) activities in the leaves under the lights of 20 000 lx and 16 000 lx for 30 d were all higher than those under the lights of 20 000 lx and 16 000 lx for 1 d.Under the light of 8 000 lx for 30 d,the growth indexes of root and leaves of grapevine seedlings were significantly lower than those of CK,and except for MDA content,most physiological and biochemical indexes of the leaves were significantly lower than those under the light of 8 000 lx for 1 d.Under12 000 lx,the values of most growth indexes in root and leaves and physiological and biochemical indexes in leaves were between the 16 000 lx and 8 000 lx.In conclusion,Yinhong could grow under the lights above 16 000 lx,and would be stunted by the weak light below 8 000 lx.展开更多
Effects of light intensity on growth, development and formation on Fagopy- rum cymosum were explored with natural light intensities at 100%(A), 85.2%(B), 75.8%(C) and 59.7%(D). The results showed that the decl...Effects of light intensity on growth, development and formation on Fagopy- rum cymosum were explored with natural light intensities at 100%(A), 85.2%(B), 75.8%(C) and 59.7%(D). The results showed that the decline of light intensity re- duced nutrient growth period, so that plant growth stage entered in advance and extended, which indicated that the decline of light intensity would lower leaf number and area, the number of twigs from the stem, as well as photosynthate. Further- more, the reduction degree increased upon light intensity decrease degree, and the proper shading improved stem height and leaf area enhanced before growth term.展开更多
A novel substrate current model is proposed for submicron and deep-submicron li ghtly-doped-drain (LDD) n-MOSFET,with the emphasis on accurate description of the characteristics length by taking the effects of channe...A novel substrate current model is proposed for submicron and deep-submicron li ghtly-doped-drain (LDD) n-MOSFET,with the emphasis on accurate description of the characteristics length by taking the effects of channel length and bias int o account.This is due to that the characteristics lenth significantly affects th e maximum lateral electric field and the length of velocity saturation region,bo th of which are very important in modeling the drain current and the substrate c urrent.The comparison between simulations and experiments shows a good predictio n of the model for submicron and deep-submicron LDD MOSFET.Moreover,the analyti cal model is suitable for descgn of devices as it is low in computation consumpt ion.展开更多
[Objective] Surveys of phytoplankton were taken during the autumn of 2009 along Anhui-Jiangsu reaches of the Yangtze River to understand the species com- position and their spatial distributions. [Method] Phytoplankto...[Objective] Surveys of phytoplankton were taken during the autumn of 2009 along Anhui-Jiangsu reaches of the Yangtze River to understand the species com- position and their spatial distributions. [Method] Phytoplankton species, their quanti- ties, biomass and the Mcnaughton's dominance indices were all measured and their spatial distribution characteristics were determined using cluster analysis. [Result] There were 27 species, belonging to 5 phyla, namely Chlorophyta, Bacillariophyta, Cyanophyta, Euglenophyta and Cryptophyta, of phytoplankton collected and identified from the surveys. Results showed that Bacillariophyta was the predominant phyto- plankton with 16 species collected which accounted for 59.3% of the total species identified. The number of species collected belonging to Chlorophyta, Cyanophyta, Cryptophyta and Euglenophyta were 6, 2, 2, and 1, accounting for 22.2%, 7.4%, 7.4% and 3.7% of the total number identified, respectively. At the species level, the predominant species were Fragilaria crotonensis, Melosira italica, Navicula crypto- cephala, Cyclotella striata and Chroomonas acuta. Phytoplankton abundance was ranging from 5.68×10^4 to 7.08×10^4 cells/L with its average of 6.01×10^4 cells/L. Phyto- plankton biomass was ranging from 30.43 to 34.73 μg/L with its average of 32.46 μg/L. Compared with the previous reports, the number of phytoplankton species was decreased but its abundance and biomass was increased along the Jiangsu reach of the Yangtze River. However, Bacillariophyta species were still the predominant species and the phytoplankton community structure had not significantly changed from the previous studies. [Conclusion] These results might be explained as that the water quality in the Yangtze River was deteriorated but had not come to the worst. The results of similarity analysis gave two clusters of phytoplankton community as Nanjing, Wuhu and Jiangyin sampling sites were clustered into one group and Tongling and Anqing were clustered into another group.展开更多
[Objective] This study was to improve the virus replication efficiency of full length infectious cDNA clones by making use of the ribozyme's self incision property.[Method] By employing three-step PCR,HDV ribozyme(H...[Objective] This study was to improve the virus replication efficiency of full length infectious cDNA clones by making use of the ribozyme's self incision property.[Method] By employing three-step PCR,HDV ribozyme(HdvRz)cDNA was isolated,and cloned into the downstream flanking the genome of the porcine reproductive and respiratory syndrome virus,and into which the bovine growth hormone polyadenylation sequence(BGH)was inserted via enzyme digestion and ligation,yielding pAPRRS-HB.The newly constructed pAPRRS-HB was used to transfect MARC-145 cells,in which the N protein and non-structural protein(nsp2)were determined by indirect immunofluorescence assay after 72 h of expression;meanwhile the virus titer of cell supernatant was tested using TCID50 assay.[Result] pAPRRS-HB containing complete infectious PRRSV cDNA has been successfully developed,and it performed about 10-fold higher virus rescue rate than pAPRRS without the engineered ribozyme element.[Conclusion] The results laid a foundation for revealing the structure and function of PRRSV gene.展开更多
Based on the 1961-2010 weather data from 65 meteorological stations in Heilongjiang Province, using the statistic software and GIS, the change characteristics of water budget, including the precipitation, reference ev...Based on the 1961-2010 weather data from 65 meteorological stations in Heilongjiang Province, using the statistic software and GIS, the change characteristics of water budget, including the precipitation, reference evapotranspiration and water budget in the whole year and the temperature-defined growing season, were analyzed. The results indicated that the both the precipitation during the whole year and that of during the temperature-defined growing season from 1961 to 2010 all decreased, while the annual reference evapotranspiration decreased, but the reference evapotranspiration during the temperature-defined growing season presented an increasing trend. The water resources were generally characterized by water deficit, especially in the beginning of 21 st .展开更多
In the clinical reports, the E1784K mutation in SCN5A is recognized as a phenotypic overlap between the long QT syndrome (LQT3) and the Brugada syndrome (BrS) in the characteristics of electrocardiograms (ECGs) ...In the clinical reports, the E1784K mutation in SCN5A is recognized as a phenotypic overlap between the long QT syndrome (LQT3) and the Brugada syndrome (BrS) in the characteristics of electrocardiograms (ECGs) since the mutation can influence sodium channel functions. However it is still unclear if the E1784K mutation-induced sodium ionic channel alterations account for the overlap at tissue level. Thsu, a detailed computational model is developed to underpin the functional impacts of the E1784K mutation on the action potential (AP), the effective refractory period (ERP) and the abnormal ECG. Simulation results stlggest'that the E1784K mutation-induced sodium channel alterations are insufficient to produce the phenotypic overlap between LQT3 and BrS, and the overlap may arise from the complicated effects of the E1784K mutation-induced changes in sodium channel currents with an increase of the transient outward current ITo or a decrease of the L-type calcium current ICaL .展开更多
[Objective] To study the cocoon traits of wild silkworm descendants. [Method] In the selective breeding of domesticated raising wild silkworm and wild silkworm descendants, investigation on the traits of the built coc...[Objective] To study the cocoon traits of wild silkworm descendants. [Method] In the selective breeding of domesticated raising wild silkworm and wild silkworm descendants, investigation on the traits of the built cocoons was carried out. [Result] The pointed traits of cocoons displayed obviously in the wild silkworm descendants, its pointed parts were consistent with the rear part of silkworm chrysalis, and the female silkworm has slight difference, indicating the silkworm co- coon pointed trait was hereditary. [Conclusion] This study provided references for the study of silkworm cocoon traits of wild silkworm descendant and guides the selective breeding of silkworm variety.展开更多
Aim Peptides as ligands have shown the active targeting properties to the receptors like integrins, a family of receptors over-expressed in cancers. The present study was to develop and characterize two peptides modif...Aim Peptides as ligands have shown the active targeting properties to the receptors like integrins, a family of receptors over-expressed in cancers. The present study was to develop and characterize two peptides modified drug-containing liposomes. Methods Argine-glycine-aspartic acid (RGD) tripeptide and glycine-argine-glycine-aspartic acid-serine (GRGDS) pentapeptide were used for modifications on the doxorubicin-loaded sterically stabilized liposomes (SSL-doxorubicin) for the liposome preparation, RGD-SSL-doxorubicin and GRGDS-SSL-doxorubicin, respectively. Characterizations were performed by measurements of the encapsulation efficiency, particle size and zeta potential, release rates in a simulated in vivo environment, and cytotoxicity to ovarian cancer cells. Cell uptake was investigated by flow cytometry and confocal microscopy methods. Results All encapsulation efficiencies of the liposomes were above 95%, and the modifications using RGD or GRGDS did not affect the final encapsulation efficiency. Average particle sizes of the liposomes Were in the range between 105.7 ± 3.5 nm and 130.5 ± 3.0 nm, and zeta potential values were between -3.3 ± 0.3 and -6.1 ± 0.3 mV. Approximately 2/5 of doxorubicin was released from liposomes before 12 h in the simulated in vivo environment containing fetal bovine serum. Inhibitory rates to cancer cells of the modified liposomes were slightly lower as compared to free doxorubicin. Similar phenomena were observed in the uptake measured by flow cytometry and confocal assay. After uptake applying various formulations on the cancer cells, doxorubicin was mainly distributed in the nuclei of SKOV-3 cells. Conclusion Two new doxorubicin-contained liposomes were successfully prepared and modified with argine-glycine-aspartic acid (RGD) tripeptide and glycine-argine-glycine- aspartic acid-serine (GRGDS) pentapeptide. In vitro characterization indicated that modifications did not alter significantly the properties of the sterically stabilized liposomes.展开更多
Multipurpose tree species (MPTs) were studied in an agroforestry arboretum under subtropical humid climate in Northeast India. Out of 12 MPTs planted under agroforestry systems, Acacia auriculiformis in spacing of 2...Multipurpose tree species (MPTs) were studied in an agroforestry arboretum under subtropical humid climate in Northeast India. Out of 12 MPTs planted under agroforestry systems, Acacia auriculiformis in spacing of 2 m × 2 m (2500 stems·hm^-2) could have the potentiality to meet the timber/fuelwood requirement due to its high wood production of 635 m^3·hm^-2 with mean annual increment (MAI) of 2.54×10^-2 m^3.treel.a^-1 in a short rotation period of 10 years. Thus, A. auriculiformis is a short rotation forest tree species suitable to grow in subtropical humid climate. On the other hand, at 16 years of age, Eucalyptus hybrid and Michelia champaca in spacing of 3 m × 3 m (1111 stems.hm^2) produced appreciably high timber volume of 315 m^3.hm^-2 and 165 m^3.hm^-2 with MAI of 1.77×10^-2 m^3.tree^-1·a^-1 and 0.92×10.2 m^3.tree^-1.a^-1, respectively. At 16 years of age, Gmelina arborea produced a timber volume of 147 m^3.hm^-2 with MAI of 1.47×10^-2 m^3.tree^-1.a^-1 followed by Samania saman (140 m^3.hm^-2), Albizziaprocera (113 m^3·hm^-2) and Tectona grandis (79 m3.hm^-2) with MAI of 1.40, 1.13 and 0.78 × 10^-2 m^3 .tree^-1a^-1, respectively in 4 m × 4 m spacing (625 stems.hm^-2). Gliricidia maculata and Leucaena leucocephala could be used as live fences around the farm boundary to supply their N-rich leaves for mulch as well as manure to crops. In agroforestry arboretum, direct seeded upland rice (Oryza sativa - variety, AR-11), groundnut (Arachis hypogaea - variety, JL-24) and sesamum (Sesamum indicum - variety, B-67) were grown during the initial period upto 8 years of tree establishment. Under other MPTs, there was a reduction in crop productivity as compared to open space. After 8 years of tree establishment, horti-silvi and silvi-pastoral systems were developed and pineapple (Ananas comosus - variety Queen), turmeric (Curcuma longa -variety RCT -1) and cowpea (Vigna sinensis - variety Pusa Barsati) as forage crop were raised. The productivity of pineapple, turmeric and cowpea was comparatively high under Azadirachta indica. The productivity of horticultural and forage crops in association with trees such as G. arborea, A. procera, S. saman, T. grandis and M. champaca of high timber value could be harnessed as viable agroforestry systems. Changes in soil properties were also monitored. Amelioration of soil acidity, increase in soil organic carbon, and enhanced humification of soil humus, high nutrient availability, low soil erodibility and high surface soil (0-15 cm) moisture availability were noted in soils under MPTs.展开更多
基金supported by the Shaanxi Province Natural Science Basic Research Plan Project(2023-JC-YB-244).
文摘The classification of infrasound events has considerable importance in improving the capability to identify the types of natural disasters.The traditional infrasound classification mainly relies on machine learning algorithms after artificial feature extraction.However,guaranteeing the effectiveness of the extracted features is difficult.The current trend focuses on using a convolution neural network to automatically extract features for classification.This method can be used to extract signal spatial features automatically through a convolution kernel;however,infrasound signals contain not only spatial information but also temporal information when used as a time series.These extracted temporal features are also crucial.If only a convolution neural network is used,then the time dependence of the infrasound sequence will be missed.Using long short-term memory networks can compensate for the missing time-series features but induces spatial feature information loss of the infrasound signal.A multiscale squeeze excitation–convolution neural network–bidirectional long short-term memory network infrasound event classification fusion model is proposed in this study to address these problems.This model automatically extracted temporal and spatial features,adaptively selected features,and also realized the fusion of the two types of features.Experimental results showed that the classification accuracy of the model was more than 98%,thus verifying the effectiveness and superiority of the proposed model.
基金Supported by National Natural Science Foundation of China(41061009)Yili Normal College Research Fund(200917)~~
文摘[Objective] The aim was to explore the growth characteristics of different quantity leaflet compound leaves of Juglans cathayensis in Xinjiang so as to provide basic data for the study of J.cathayensis.[Method] The length,width and biomass of compound leaves growth characteristics of three types of different quantity leaflet compound leaves of J.cathayensis were measured in large samples.[Result] The length,width,rachis biomass and total biomass of compound leaves increased with the number of leaflets increase;leaflets biomass of the same phyllotaxy decreased with the number of leaflets increase.The leaflet length,width and biomass of the same compound leaves increased with the number of phyllotaxy increase.The differences on petiole length and top leaflets length were not significant;there were significant differences on compound leaves width;the differences on the total biomass of compound leaves were extremely significant.[Conclusion] There was rather large ecology plasticity of the compound leaves of J.cathayensis in Xinjiang.
文摘Glyptosternoid fishes are a group of sisorid catfishes living in torrents of rivers mainly originating from the Qinghai-Tibet Plateau. Based on our survey in the Drung River Basin, seven collecting sites were investigated and 271 glyptosternoid fishes caught belong to three species (Pareuchiloglanis kamengensis, Exostoma labiatum and Oreoglanis mocropterus). Features of the distribution of the three catfishes were assessed. More individuals of E. labiatum were caught in the lower reaches of the Drung River with fast water velocity and it might be more adapted to a torrent habitat. The relationships between standard length (L) and weight (W) for P. kamengensis, E. labiatum and O. macropterus were also studied, and the parameter b of the L-W relationship (W = aL^b) ranged between 2. 8201 and 3. 0131. From the present study, all the three catfish species grow allometrically and the growth type of E. labiatum is the closest to a symmetrical one.
文摘Based on the clay minerals, content analysis from 187 stations in the surface sediments, this paper dealt with the assemblages and the distributions of clay minerals in the surface sediments off the Yangtze River estuary. The research results showed that the illite dominates the surface sediments off the Yangtze River estuary with the smectite or kaolinite the second rank and chlorite the third rank; the illite-smectite-kaolinite-chlorite-assemblage is the main type of the clay minerals assemblage in the area, with the illite-kaolinite-chlorite-smectite-type as the second rank; the source of clay mineral is mainly from the Yellow River and the Yangtze River. The Q-type cluster analysis show that sediments from both recent and ancient Yellow River were deposited mainly in the northeast part of the study area to the north of 29°30′. Substance from the Yangtze River was deposited mainly in the western and middle parts off the Yangtze River estuary.
基金supported by the National 863 Program (2002BA515B0401)National Natural Science Foundation of China (30571513)Foundation of Heilongjiang Province (GA06B301)
文摘The fiber length trait (FLT) of 538 individuals from nature birch population in Maorshan region, Heilongjang, China were measured, of which 100 individuals were selected as representative variety of correlated fragments screening with random amplified polymorphism DNA (RAPD) technique. In total of 20 RAPD primers were tested through multiple regression analysis between amplified strip and the character behaviors, and a correlative segment BFLR-16 was obtained. The correlation coefficient between BFLI-16 and FLT was 0.6144, with the significant level of 1%. BFLI-16 was then cloned, sequenced and transformed into SCAR marker. The percentage of identifying long fiber birches by this SCAR was more than 92. The result indicates that the SCAR markers has high specificity for the long fiber individuals and is highly linked with the gene controlling the character of fiber length, and its existence is significantly correlative with the increase in the fiber length.
基金Project(201206375003)supported by the China Scholarship Council
文摘SiC nanowires were prepared on C/C composite surface without catalyst by chemical vapor deposition(CVD) using CH3 SiCl3 as precursor.SEM images of the CVD-product reveal that some long nanowires have grown to tens of micrometers with some gathered as a ball.Some short nanowires agglomerate like chestnut shell with many thorns accompanied by some deposited nano-particles.XRD,Raman-spectrum and FTIR patterns indicate that the product is a typical β-SiC.TEM images show that the nanowires have a wide diameter range from 10 to 100 nm,and some thin nanowires are bonded to the thick one by amorphous CVD-SiC.A SiC branch generates from an amorphous section of a thick one with an angle of 70° between them,which is consistent with the [111] axis stacking angle of the crystal.SAED and fast Fourier transform(FFT) patterns reveal that the nanowires can grow along with different axes,and the bamboo-nodes section is full of stacking faults and twin crystal.The twisted SiC lattice planes reveal that the screw dislocation growth is the main mechanism for the CVD-SiC nanowires.
基金Supported by Key Project of Ministry of Agriculture([2015]No.6)~~
文摘The research analyzed birthplace, work site and Work unit, discipline, agri- cultural product variety, current age, and selected age, as well as internal and exter- nal factors for growth, which providers references for exploring development of high- level agricultural S&T talents and formulation of relevant policies.
基金Supported by Jiangsu Self-innovation Fund for Agricultural Science and Technology[CX(12)2008]~~
文摘The growth and development of Jingjiang Taro [Colocasia esculenta(L.) Schott] were observed, laying foundation for highly-yielding cultivation technology system of Xiangsha taro. The results indicate that germination of the second taro happened from 46 d after sprouting (7-8 leaves); the first taro began to expand, from 125 d after sprouting (12 leaves) when the the second taro was been formed; the third taro began to form from 141 to 150 d after sprouting (15-16 leaves). The fresh weight of the second and third taros quickly increased from the time of 15 leaves to harvest. Early September is a transformation term of Xiangsha taro from vegetative body dominant to corm growth dominant.
基金Supported by Science and Technology Innovation Team Project of Ningbo Province of China(2011B82019)Supported by Natural Science Foundation of Ningbo Province of China(Y13C150014)+1 种基金Supported by Foundation of Zhejiang Educational Committee(2011C32008)Supported by Science and Technology Project of Ningbo Province,China(2010C91049)~~
文摘The plant growth and physiological and biochemical responses of root and leaves of grape Yinhong seedlings to the weak lights of 20 000,16 000,12 000,8 000 lx and the normal illumination of 25 000 lx(CK) respectively,were investigated.There was no significant dfference in growth indexes of root and leaves of grapevine seedlings between the light of 20 000 lx and CK,and the light of 16 000 lx and CK for 30 d.The chlorophyll contents,soluble protein contents,net photosynthetic rates,transpiration rates,stomatal conductance,water use efficiency and protective enzyme(CAT,POD,SOD) activities in the leaves under the lights of 20 000 lx and 16 000 lx for 30 d were all higher than those under the lights of 20 000 lx and 16 000 lx for 1 d.Under the light of 8 000 lx for 30 d,the growth indexes of root and leaves of grapevine seedlings were significantly lower than those of CK,and except for MDA content,most physiological and biochemical indexes of the leaves were significantly lower than those under the light of 8 000 lx for 1 d.Under12 000 lx,the values of most growth indexes in root and leaves and physiological and biochemical indexes in leaves were between the 16 000 lx and 8 000 lx.In conclusion,Yinhong could grow under the lights above 16 000 lx,and would be stunted by the weak light below 8 000 lx.
基金Special Funds for Fundamental Work of Ministry of Science and Technology(2006BAD13B02-13)~~
文摘Effects of light intensity on growth, development and formation on Fagopy- rum cymosum were explored with natural light intensities at 100%(A), 85.2%(B), 75.8%(C) and 59.7%(D). The results showed that the decline of light intensity re- duced nutrient growth period, so that plant growth stage entered in advance and extended, which indicated that the decline of light intensity would lower leaf number and area, the number of twigs from the stem, as well as photosynthate. Further- more, the reduction degree increased upon light intensity decrease degree, and the proper shading improved stem height and leaf area enhanced before growth term.
文摘A novel substrate current model is proposed for submicron and deep-submicron li ghtly-doped-drain (LDD) n-MOSFET,with the emphasis on accurate description of the characteristics length by taking the effects of channel length and bias int o account.This is due to that the characteristics lenth significantly affects th e maximum lateral electric field and the length of velocity saturation region,bo th of which are very important in modeling the drain current and the substrate c urrent.The comparison between simulations and experiments shows a good predictio n of the model for submicron and deep-submicron LDD MOSFET.Moreover,the analyti cal model is suitable for descgn of devices as it is low in computation consumpt ion.
基金Supported by Fishery Germplasm Conservation Project of the Ministry of Agriculture(MOA)(No.6115048)State Specific Project on Fundamental Scientific Research Financed to Public Institutes(No.2009JBFB10)~~
文摘[Objective] Surveys of phytoplankton were taken during the autumn of 2009 along Anhui-Jiangsu reaches of the Yangtze River to understand the species com- position and their spatial distributions. [Method] Phytoplankton species, their quanti- ties, biomass and the Mcnaughton's dominance indices were all measured and their spatial distribution characteristics were determined using cluster analysis. [Result] There were 27 species, belonging to 5 phyla, namely Chlorophyta, Bacillariophyta, Cyanophyta, Euglenophyta and Cryptophyta, of phytoplankton collected and identified from the surveys. Results showed that Bacillariophyta was the predominant phyto- plankton with 16 species collected which accounted for 59.3% of the total species identified. The number of species collected belonging to Chlorophyta, Cyanophyta, Cryptophyta and Euglenophyta were 6, 2, 2, and 1, accounting for 22.2%, 7.4%, 7.4% and 3.7% of the total number identified, respectively. At the species level, the predominant species were Fragilaria crotonensis, Melosira italica, Navicula crypto- cephala, Cyclotella striata and Chroomonas acuta. Phytoplankton abundance was ranging from 5.68×10^4 to 7.08×10^4 cells/L with its average of 6.01×10^4 cells/L. Phyto- plankton biomass was ranging from 30.43 to 34.73 μg/L with its average of 32.46 μg/L. Compared with the previous reports, the number of phytoplankton species was decreased but its abundance and biomass was increased along the Jiangsu reach of the Yangtze River. However, Bacillariophyta species were still the predominant species and the phytoplankton community structure had not significantly changed from the previous studies. [Conclusion] These results might be explained as that the water quality in the Yangtze River was deteriorated but had not come to the worst. The results of similarity analysis gave two clusters of phytoplankton community as Nanjing, Wuhu and Jiangyin sampling sites were clustered into one group and Tongling and Anqing were clustered into another group.
基金Supported by National Science and Technology R&D Program during 11th 5-year Plan Period(2006BAD06A01)~~
文摘[Objective] This study was to improve the virus replication efficiency of full length infectious cDNA clones by making use of the ribozyme's self incision property.[Method] By employing three-step PCR,HDV ribozyme(HdvRz)cDNA was isolated,and cloned into the downstream flanking the genome of the porcine reproductive and respiratory syndrome virus,and into which the bovine growth hormone polyadenylation sequence(BGH)was inserted via enzyme digestion and ligation,yielding pAPRRS-HB.The newly constructed pAPRRS-HB was used to transfect MARC-145 cells,in which the N protein and non-structural protein(nsp2)were determined by indirect immunofluorescence assay after 72 h of expression;meanwhile the virus titer of cell supernatant was tested using TCID50 assay.[Result] pAPRRS-HB containing complete infectious PRRSV cDNA has been successfully developed,and it performed about 10-fold higher virus rescue rate than pAPRRS without the engineered ribozyme element.[Conclusion] The results laid a foundation for revealing the structure and function of PRRSV gene.
基金Supported by the Special Fund for Meteorological-Scientific Research in the Public Interest(GYHY201106026,GYHY200906021,GYHY200706030)the Special Fund for Climate Changes of China Meteorological Administration(CCSF-09-13)~~
文摘Based on the 1961-2010 weather data from 65 meteorological stations in Heilongjiang Province, using the statistic software and GIS, the change characteristics of water budget, including the precipitation, reference evapotranspiration and water budget in the whole year and the temperature-defined growing season, were analyzed. The results indicated that the both the precipitation during the whole year and that of during the temperature-defined growing season from 1961 to 2010 all decreased, while the annual reference evapotranspiration decreased, but the reference evapotranspiration during the temperature-defined growing season presented an increasing trend. The water resources were generally characterized by water deficit, especially in the beginning of 21 st .
基金Supported by the National Natural Science Foundation of China(61001167,61172149)~~
文摘In the clinical reports, the E1784K mutation in SCN5A is recognized as a phenotypic overlap between the long QT syndrome (LQT3) and the Brugada syndrome (BrS) in the characteristics of electrocardiograms (ECGs) since the mutation can influence sodium channel functions. However it is still unclear if the E1784K mutation-induced sodium ionic channel alterations account for the overlap at tissue level. Thsu, a detailed computational model is developed to underpin the functional impacts of the E1784K mutation on the action potential (AP), the effective refractory period (ERP) and the abnormal ECG. Simulation results stlggest'that the E1784K mutation-induced sodium channel alterations are insufficient to produce the phenotypic overlap between LQT3 and BrS, and the overlap may arise from the complicated effects of the E1784K mutation-induced changes in sodium channel currents with an increase of the transient outward current ITo or a decrease of the L-type calcium current ICaL .
基金Supported by the Agricultural Fund Project of Ministry of Science and Technology(Y0415)the China Agriculture Research System(Sericulture)~~
文摘[Objective] To study the cocoon traits of wild silkworm descendants. [Method] In the selective breeding of domesticated raising wild silkworm and wild silkworm descendants, investigation on the traits of the built cocoons was carried out. [Result] The pointed traits of cocoons displayed obviously in the wild silkworm descendants, its pointed parts were consistent with the rear part of silkworm chrysalis, and the female silkworm has slight difference, indicating the silkworm co- coon pointed trait was hereditary. [Conclusion] This study provided references for the study of silkworm cocoon traits of wild silkworm descendant and guides the selective breeding of silkworm variety.
基金National Natural Science Foundation of China(Grant No. 30572261)the 985 Projects (Phase II) of theState Key Laboratory of Natural and Biomimetic Drugs(Peking University, China).
文摘Aim Peptides as ligands have shown the active targeting properties to the receptors like integrins, a family of receptors over-expressed in cancers. The present study was to develop and characterize two peptides modified drug-containing liposomes. Methods Argine-glycine-aspartic acid (RGD) tripeptide and glycine-argine-glycine-aspartic acid-serine (GRGDS) pentapeptide were used for modifications on the doxorubicin-loaded sterically stabilized liposomes (SSL-doxorubicin) for the liposome preparation, RGD-SSL-doxorubicin and GRGDS-SSL-doxorubicin, respectively. Characterizations were performed by measurements of the encapsulation efficiency, particle size and zeta potential, release rates in a simulated in vivo environment, and cytotoxicity to ovarian cancer cells. Cell uptake was investigated by flow cytometry and confocal microscopy methods. Results All encapsulation efficiencies of the liposomes were above 95%, and the modifications using RGD or GRGDS did not affect the final encapsulation efficiency. Average particle sizes of the liposomes Were in the range between 105.7 ± 3.5 nm and 130.5 ± 3.0 nm, and zeta potential values were between -3.3 ± 0.3 and -6.1 ± 0.3 mV. Approximately 2/5 of doxorubicin was released from liposomes before 12 h in the simulated in vivo environment containing fetal bovine serum. Inhibitory rates to cancer cells of the modified liposomes were slightly lower as compared to free doxorubicin. Similar phenomena were observed in the uptake measured by flow cytometry and confocal assay. After uptake applying various formulations on the cancer cells, doxorubicin was mainly distributed in the nuclei of SKOV-3 cells. Conclusion Two new doxorubicin-contained liposomes were successfully prepared and modified with argine-glycine-aspartic acid (RGD) tripeptide and glycine-argine-glycine- aspartic acid-serine (GRGDS) pentapeptide. In vitro characterization indicated that modifications did not alter significantly the properties of the sterically stabilized liposomes.
文摘Multipurpose tree species (MPTs) were studied in an agroforestry arboretum under subtropical humid climate in Northeast India. Out of 12 MPTs planted under agroforestry systems, Acacia auriculiformis in spacing of 2 m × 2 m (2500 stems·hm^-2) could have the potentiality to meet the timber/fuelwood requirement due to its high wood production of 635 m^3·hm^-2 with mean annual increment (MAI) of 2.54×10^-2 m^3.treel.a^-1 in a short rotation period of 10 years. Thus, A. auriculiformis is a short rotation forest tree species suitable to grow in subtropical humid climate. On the other hand, at 16 years of age, Eucalyptus hybrid and Michelia champaca in spacing of 3 m × 3 m (1111 stems.hm^2) produced appreciably high timber volume of 315 m^3.hm^-2 and 165 m^3.hm^-2 with MAI of 1.77×10^-2 m^3.tree^-1·a^-1 and 0.92×10.2 m^3.tree^-1.a^-1, respectively. At 16 years of age, Gmelina arborea produced a timber volume of 147 m^3.hm^-2 with MAI of 1.47×10^-2 m^3.tree^-1.a^-1 followed by Samania saman (140 m^3.hm^-2), Albizziaprocera (113 m^3·hm^-2) and Tectona grandis (79 m3.hm^-2) with MAI of 1.40, 1.13 and 0.78 × 10^-2 m^3 .tree^-1a^-1, respectively in 4 m × 4 m spacing (625 stems.hm^-2). Gliricidia maculata and Leucaena leucocephala could be used as live fences around the farm boundary to supply their N-rich leaves for mulch as well as manure to crops. In agroforestry arboretum, direct seeded upland rice (Oryza sativa - variety, AR-11), groundnut (Arachis hypogaea - variety, JL-24) and sesamum (Sesamum indicum - variety, B-67) were grown during the initial period upto 8 years of tree establishment. Under other MPTs, there was a reduction in crop productivity as compared to open space. After 8 years of tree establishment, horti-silvi and silvi-pastoral systems were developed and pineapple (Ananas comosus - variety Queen), turmeric (Curcuma longa -variety RCT -1) and cowpea (Vigna sinensis - variety Pusa Barsati) as forage crop were raised. The productivity of pineapple, turmeric and cowpea was comparatively high under Azadirachta indica. The productivity of horticultural and forage crops in association with trees such as G. arborea, A. procera, S. saman, T. grandis and M. champaca of high timber value could be harnessed as viable agroforestry systems. Changes in soil properties were also monitored. Amelioration of soil acidity, increase in soil organic carbon, and enhanced humification of soil humus, high nutrient availability, low soil erodibility and high surface soil (0-15 cm) moisture availability were noted in soils under MPTs.