地肤叶水提物对四氧嘧啶致糖尿病小鼠降糖作用的观察

目的:探讨地肤叶水提物对糖尿病小鼠的降糖作用。方法:通过标准化四氧嘧啶造模糖尿病小鼠。选取血糖相近的小鼠分3组进行实验,分别喂药10天、10天、7天后观察实验组降糖结果,进行统计分析;并对实验小鼠进行毒理学实验。结果:地肤叶水提物剂量在4·0g/kg灌胃给药,7～10日均有一定降糖作用,与对照组比较可下降20%左右,最高达34·5%。结论:地肤叶水提物具有一定降糖作用,且不具有明显的毒副作用。

地肤子正丁醇部分降糖机制的研究

目的 :观察地肤子正丁醇部分 (NBFK)对小鼠胃排空、大鼠小肠黏膜α 葡萄糖苷酶活性及小肠糖吸收的影响 ,研究其降糖作用机制。方法 :小鼠灌胃酚红测定胃中酚红残留量计算胃排空百分率 ,并按葡萄糖氧化酶法测定血糖含量 ;在大鼠小肠黏膜匀浆中加入蔗糖、麦芽糖或乳糖 ,测产生的葡萄糖量以表示小肠黏膜α 葡萄糖苷酶的活性 ;大鼠小肠灌流含葡萄糖液 ,测灌流液中葡萄糖的浓度 ,计算未吸收的葡萄糖量。结果 :NBFK 5 0mg/kg灌胃能抑制正常小鼠胃排空 ,2 5、5 0mg/kg明显抑制灌胃葡萄糖、静脉注射四氧嘧啶所致高血糖小鼠和皮下注射胰岛素所致低血糖小鼠的胃排空 ;NBFK 12 5～ 5 0 0 μg/ml抑制大鼠小肠粘膜蔗糖酶、麦芽糖酶和乳糖酶的活性 ,10 0～ 80 0 μg/ml浓度依赖性减少大鼠小肠对葡萄糖的吸收。 结论 :地肤子正丁醇部分的降糖作用与其抑制糖分在胃肠道的转运、转化及吸收有关。

地肤子正丁醇部分对小鼠小肠运动的影响

目的 :研究地肤子正丁醇部分 (NBFK)灌胃给药对小鼠小肠推进功能的影响。方法 :以炭末在小鼠小肠内的推进率为指标 ,在正常小鼠及芬氟拉明、多巴胺等预处置小鼠 ,观察NKFK对小肠运动的影响。结果 :NBFK 5 0mg/kg促进正常小鼠的小肠推进功能 ,芬氟拉明、多巴胺、醋酸和Nω 硝基 L 精氨酸抑制肠运动后 ,其促进作用更加明显 ,而阿托品预处置则使其促进作用消失。结论 :NBFK改善小肠推进功能 。

Comparison and Evaluation of Several Dermatophagoides Pteronyssinus Allergen Extracts for Skin Prick Test

Objective To evaluate the significance of several Dermatophagoides pteronyssinus allergen extracts for skin prick test(SPT) in patients allergic to Dermatophagoides pteronyssinus.Methods Two hundred and nineteen patients enrolled in Peking Union Medical College Hospital underwent SPT and serum specific IgE assay to detect the Dermatophagoides pteronyssinus allergen.Three kinds of house dust mite allergen extracts were used for SPT,including the Dermatophagoides pteronyssinus extract prepared by our laboratory(group A),standardized Dermatophagoides pteronyssinus extract(group B),and mixed extracts of Dermatophagoides pteronyssinus and Dermatophagoides farinae(group C).Human serum specific IgE result was regarded as the reference standard for diagnosis of Dermatophagoides pteronyssinus allergy.The receiver operating characteristic(ROC) curve was used to evaluate the diagnostic performance of SPT with the extracts of three groups.Results SPT results showed that the median wheal diameter of group A,group B,and group C was 0.43,0.35,and 0.28 cm,respectively,with significant difference among three groups(P<0.05).The difference was significant between group A and B(P<0.01) as well as group A and C(P<0.01),but not between group B and C(P>0.05).There was no local urticaria or systemic allergic reactions following the procedure of SPT.Local reaction was observed in 5 patients and delayed reaction was in 2 patients of group A.As for group B and C,local reaction occurred in 3 cases and delayed reaction in 2 cases in each group.The area under ROC curve of SPT with extract in group A,group B,and group C was 0.765,0.801,and 0.782,respectively.Based on the detection results of serum specific IgE,the sensitivity of SPT in diagnosis of Dermato-phagoides pteronyssinus allergy with extract of group A,group B,and group C was 92.4%,87.0%,and 81.5%,and the specificity was 60.6%,73.2%,and 74.8%,respectively.Conclusion The Dermatophagoides pteronyssinus extract for SPT prepared by our laboratory offers good sensitivity and specificity comparable to commercially available allergen extracts,and it may be an appropriate candidate for clinical screening and diagnosis of Dermatophagoides pteronyssinus allergy.