整合子在产ESBLs志贺菌中的检测

目的:探讨产超广谱β-内酰胺酶(ESBLs)志贺菌多重耐药与整合子的关系,研究其耐药机制。方法:圆盘扩散方法检测抗菌药物敏感性,改良三维试验对可疑产ESBLs志贺菌进行检测,多重PCR检测、、类整合子,并对整合子进行DNA测序以确定携带的耐药基因盒。结果:在10株产ESBLs志贺菌中全部检出类整合子,其可变区含有dfrA17-aadA5基因盒。结论:本地区产ESBLs志贺菌全部含有类整合子,分别编码磺胺类和氨基糖苷类多重耐药性。

Survey of Multidrug Resistance and Class I Integron Prevalence in Chicken-derived Salmonella

[Objective] This study aimed to investigate the multidrug resistance and prevalence of class I integrons in Salmonel a. [Method] Salmonel a strains were isolated from chicken farms in Shandong Province. Kauffmann-White classification method was employed to analyze the serotypes of Salmonel a strains. Minimum in-hibition concentration （MIC） of Salmonel a strains against 19 common antimicrobial drugs was analyzed determined with microdilution method. The class I integrons and carried drug resistance gene cassettes were detected by PCR. [Result] A total of 311 Salmonel a strains were isolated and classified into two serotypes, including 133 Salmonel a Indiana strains and 178 Salmonel a Enteritidis strains. Drug sensitivity test showed that the isolated Salmonel a strains were general y resistant to sulfadiazine, sulfamethoxazole, nalidixic acid, ampicil in, tetracycline, doxycycline and trimethoprim, with a multidrug resistance rate of 91.0% （283/311）; 99% strains were sensitive to amikacin and colistin. PCR assay indicated that the detection rate of class I integrons was 65.0% （202/311）; the positive rate of class I integrons in Salmonel a strains with multidrug resistance was 92.6%; among 202 positive strains, six strains carried gene cassette dfr17-aadA5. [Conclusion] According to the above results, class I integrons exist general y in Salmonel a and are closely associated with the multidrug resistance of Salmonel a strains.

In vitro investigation of RGD-modified stabilized cationic liposomes as non-viral vehicle for siRNA delivery

In this study, the novel RGD-modified stabilized cationic liposomes were developed as the delivery vehicle for siRNA targeting human MDR1 gene. The complex of cationic liposomes and siRNA, RGD-Lipo-siRNA, was prepared with a narrow size distribution below 200 nm. It was shown that the encapsulated siRNA in the liposomes could be effectively protected from serum degradation. Also, enhanced cell binding and intracellular uptake of siRNA in the doxorubicin-resistant human ova- rian cancer cell lines SKOV3/A were found in RGD-Lipo-siRNA preparation as compared to that of unmodified cationic lipsomes （Lipo-siRNA）. Using the post-insertion method for RGD modification, lysosome release of siRNA in pRGD-Lipo-siRNA was improved. From flow cytometry, significant increase of doxorubicin accumulation was observed in the SKOV3/A cells treated with pRGD-Lipo-siRNA targeting human MDR1 gene. In vitro cytotoxicity assay showed that the significant cell growth inhibition was achieved in the SKOV3/A cells after treating with the combined use of siRNA and doxorubicin. In conclusions, postinserted RGD modified lipoplex, pRGD-Lipo-siRNA, was successfully used for siRNA transfection and achieved drug resistance reversal in human ovarian cancer SKOV3/A （doxorubicin-resistant） cells. It suggested that this liposomes might be a potential vehicle for siRNA delivery in vivo.

A Property Based Approach for Simultaneous Process and Molecular Design

In this work, property clustering techniques and group contribution methods are combined to enable simultaneous consideration of process performance requirements and molecular property constraints. Using this methodology, the process design problem is solved to identify the property targets corresponding to the desired process performance. A significant advantage of the developed methodology is that for problems that can be satisfactorily described by only three properties, the process and molecular design problems can be simultaneously solved visually on a ternary diagram, irrespective of how many molecular fragments are included in the search space. On the ternary cluster diagram, the target properties are represented as individual points if given as discrete values or as a region if given as intervals. The structure and identity of candidate components is then identified by combining or ＂mixing＂ molecular fragments until the resulting properties match the targets.

Effect of Quantum Fluctuation on Two-Dimensional Spatially Anisotropic Heisenberg Antiferromagnet with Integer Spin

In the present paper, we calculate the Gaussian correction to the critical value J^c⊥ caused by quantum spin fluctuation in a two-dimensional spatially anisotropic Heisenberg antiferromagnet with integer spin S. Previously, some authors computed this quantity by the mean-field theory based on the Sehwinger boson representation of spin operators. However, for S = 1, their result is much less than the one derived by numerical calculations. By taking the effect of quantum spin fluctuation into consideration, we are able to produce a greatly improved result.

Transformation of insect-resistant gene into cauliflower (Brassica oleracea L.var. botrytis)

Cowpea Trypsin Inhibitor (CpTI) gene was transferred into the cotyle dons and hypocotyls of cauliflower by Agrobacterium-mediated transformation met hod. The best selective concentration of kanamycin (kan) was 15 mg L-1. The con centration of carbencillin (carb) was 500 mg L-1. 14 transgenic cauliflower pla nts were obtained. The putative transformants were assayed by PCR and Southern b lotting analysis. The results indicated that CpTI gene was transferred into caul iflower successfully.

Photonics-to-electronics integration for optical interconnects in the early 21^(st) century

We discuss the technical rationale,challenges,and potential for achieving “optics-to-the chip” via the intimate integration of photonics components such as lasers,detectors,and modulators with VLSI electronics.We review the progress made towards commercializing this technology for high-density optical transceivers and switching products.

Hybrid particle swarm optimization with chaotic search for solving integer and mixed integer programming problems

A novel chaotic search method is proposed,and a hybrid algorithm combining particle swarm optimization(PSO) with this new method,called CLSPSO,is put forward to solve 14 integer and mixed integer programming problems.The performances of CLSPSO are compared with those of other five hybrid algorithms combining PSO with chaotic search methods.Experimental results indicate that in terms of robustness and final convergence speed,CLSPSO is better than other five algorithms in solving many of these problems.Furthermore,CLSPSO exhibits good performance in solving two high-dimensional problems,and it finds better solutions than the known ones.A performance index(PI) is introduced to fairly compare the above six algorithms,and the obtained values of(PI) in three cases demonstrate that CLSPSO is superior to all the other five algorithms under the same conditions.

Correlation of the antiseptic resistance in Acinetobacter baumannii and the antiseptic resistance gene qacEΔ1 located in classⅠintegron

In the past decade, uses of antiseptics and disinfectants in hospitals and other health care centers are rather common, but the chance to develop resistance to antiseptics and disinfectants is also increased. Acinetobacter baumannii is one of the opportunistic bacteria involving in the nosocomial infection. In the present study, the correlation of the antiseptic resistance in A. baumannii and the antiseptic resistance gene qacEΔ 1 was investigated by means of determination of MICs. Meanwhile, the MICs of glutaraldehyde, chlorhexidine, benzalkonium bromide, iodophor and trichloroisocyanurate to 80 clinical isolates of A. baumannii were detected by tube dilution assay and the resistance genes intll and qacE Δ 1 in these isolates were amplified by PCR and verified by DNA sequencer. It was found that the MIC50 for these 5 antiseptics tested were 32, 8, 8, 4 and 1 μg/ml respectively, and the detection rates of intll and qacEΔ1 gene were 60.0% and 77.6% respectively. In addition, 55% of the 80 isolates simultaneously possessed both intll and qacEΔ1 gene, and the percentage of antiseptic resistance of A. baumannii carring both genes to benzalkonium bromide were higher than that without these two genes, however, there was no significant difference between intll and qacEΔ1 gene. The result in bactericidal efficiency assay indicated that chlorhexidine could still produce rapid and strong bactericidal effect at concentration of 1 MIC after 10 rain exposure. These results suggest that the antiseptic resistance of A. baumannil to various antiseptics is correlated with the presence of the antiseptic resistance genes qacEΔ 1 in bacteria, thus warning that the increase of the antiseptic resistance should not be ignored and the relative high concentration or prolonged application time is required to achieve a sufficient bactericidal effect.

How the Orbital System of Particles Consolidates Gravity and Electromagnetism

There is an actual reality that underlies the relative reality of physics. The orbital system is shown to be the principle by which motion transforms space into matter. The support of the universe is the absolute three-stage hierarchy of particles, atoms, and gravitational systems. Below 1/c waves are dissociated into strands and neutrinos are separated as points of charge. The electron and positron are single strands with opposed helical turns. Protons and neutrons have a nucleus of positrinos and negatrinos surrounded by concentric shells of strands in 2＂~ resonance. The orbital strands reverberate into space creating a field with gravitational and electromagnetic aspects. The orbital system defines matter, energy, motion, and time based on composition. The three stages have a constant field content but differ by field density and components.

Matrix Tensor Product Approach to the Equivalence of Multipartite States under Local Unitary Transformations

The equivalence of multipartite quantum mixed states under local unitary transformations is studied. A criterion for the equivalence of non-degenerate mixed multipartite quantum states under local unitary transformations is presented.

Analysis on the Development of Lushan （Xingzi） Hot Spring Health Tourism Products from the Perspective of Product Integrated Marketing

Through the development of more than 60 years, the hot spring tourism industry in China will confront with a situation of ＂innovative competition, standard development, and secondary upgrade＂. In this paper, from the perspective of product integrated marketing theory, by taking Lushan （Xingzi） hot spring as an example, the authors carry out an analysis on some problems in the development of Xingzi hot spring tourism products, propose the framework and model of the integrated development of hot spring health tourism products, and also provide some suggestions for the design of Xingzi hot spring health tourism routes.

Power Generation Expansion Planning Using an Interior Point with Cutting Plane （IP/CP） Method

The generation expansion planning is one of complex mixed-integer optimization problems, which involves a large number of continuous or discrete decision variables and constraints. In this paper, an interior point with cutting plane （IP/CP） method is proposed to solve the mixed-integer optimization problem of the electrical power generation expansion planning. The IP/CP method could improve the overall efficiency of the solution and reduce the computational time. Proposed method is combined with the Bender＇s decomposition technique in order to decompose the generation expansion problem into a master investment problem and a slave operational problem. The numerical example is presented to compare with the effectiveness of the proposed algorithm.

Common Information Model： A Bus Service for Electric Calculations in AES Eletropaulo

This paper resumes a research project developed in the concession area of AES Eletropaulo, the largest electrical energy distribution company in Brazil. First, the global standards of information exchange within power transmission and distribution area were evaluated, allowing the definition of state of the art on the theme, followed by determining its applications considering technologies already applied by the company. The specifications needed for the generation of a data integration model are adapted to radial overhead network at company concession area. The project developed an intermediary connectivity layer, based on the CIM （common information model）, which enables corporative systems to communicate in a standard way, through the use of integrating technologies. It, therefore, enabled modeling all main subjects of an electrical network in an open, extensible and non-proprietary way, in a model that contains classes and attributes of such subjects, as well as their relationships. Calculation and planning products adopted by the company were integrated to the technological layer implemented.

IPSO-based hybrid approaches for reliability-redundancy allocation problems

The problem of maximizing system reliability through component reliability choices and component redundancy is called tell-ability-redundancy allocation problem （RAP）, and it is a difficult but realistic nonlinear mixed-integer optimization prob- lem. For the RAP. we pay attention to an improved particle swarm optimization （IPSO）, and introduce four hybrid approaches for combining the IPSO with other conventional search techniques, such as harmony search （HS） and LXPM （a real coded GA）. The basic structure of the hybrid approaches includes two phases. After devising an initial solution by the HS or LXPM technique in the first phase, the IPSO performs an optimal search in the next phase. In addition, a new procedure by using golden search, named GS, is developed for further improving the solutions obtained by IPSO. Consequently, four ISPO-based hybrid approaches are proposed including HS-IPSO, LXPM-IPSO, HS-IPSO-GS, and LXPM-IPSO-GS. In order to validate the per-formance of proposed approaches, five nonlinear mixed-integer RAPs are investigated where both the number of re- dundancy components and the corresponding component reliability in each subsystem are to be decided simultaneously. As shown, the proposed approaches are all superior in terms of both optimal solutions and robustness to those by IPSO. Especially the pro-posed LXPM-IPSO-GS has shown more excellent performance than other typical approaches in the literature.

Interaction of citrate with Pt(100) surface investigated by cyclic voltammetry towards understanding the structure-tuning effect in nanomaterials synthesis

This study aims to understand the effects of functional agents such as capping agents, stabilizers, surfactants and additives in shape-controlled synthesis of nanomaterials. The well-defined Pt(100) single crystal surface was used as a model to investigate its interaction with citrate, a capping agent that is often used in shape-controlled synthesis of nanomaterials. It demonstrated that, through a systematic study of electrochemical cyclic voltammetry, the presence of citrate in solution could increase the current peak density of hydrogen adsorption at high potential (j p,L ), while decrease proportionally the current peak density of hydrogen adsorption at low potential (j p,S ). Furthermore, the increase of citrate concentration shifted negatively the peak potentials (E p,L and E p,S ) of both j p,L and j p,S . The results indicated that the interaction of citrate with Pt(100) surface could induce increasing the (100) surface domains of two-dimensional long range order (2D-(100)), and decreasing the (100) surface domains of one-dimensional short range order (1D-(100)). It also revealed that the interaction of citrate with Pt(100) surface could stabilize the 2D-(100) structure. The findings gained in this study implied that the citrate may lead to form stable 2D-(100) domains on Pt nanoparticles upon the shape-controlled synthesis of Pt nanomaterials.