The beginning of the Japanese Upper Paleolithic has mainly been examined using two major models:the Middle Paleolithic evolutionary model within the archipelago and the continental Upper Paleolithic diffusion/migratio...The beginning of the Japanese Upper Paleolithic has mainly been examined using two major models:the Middle Paleolithic evolutionary model within the archipelago and the continental Upper Paleolithic diffusion/migration model.However,recent archeological data from Japan and nearby countries are challenging such simple models.This paper critically reviews previous chronology of the Japanese Paleolithic,including possible Lower and Middle Paleolithic(LP/MP),and attempts to show an alternative model of the beginning of the Japanese Upper Paleolithic.This paper suggests several possible specimens of LP/MP and recommends further geoarchaeological investigation to understand the reliability and cultural relationship between possible LP/MP specimens and the Early Upper Paleolithic(EUP).The start of the Japanese EUP is presently characterized by a flake industry with trapezoids and denticulates around 39-37 kaBP cal on Paleo-Honshu Island,which has partial resemblance with contemporary assemblages in China and the Korean Peninsula,although trapezoids are endemic only to the Japanese EUP and may have derived from the ancestral lithic tradition.Blade technology appeared earliest on Central Paleo-Honshu Island,about 1000 years later than the earliest flake technology.Although blade technology may have originated from the elongated flake technology of the previous period,the sudden simultaneous emergence implies that it diffused from the Korean Peninsula.This paper proposes that blade technology from the Korean Peninsula arrived on the northeastern Paleo-Honshu Island,including the Japan Sea coastal region of western Honshu,rather than the southwest,where flake technology long prospered,due to differences in ecological settings and adaptation strategies between the two regions.展开更多
This study analyzed nueleotide sequences from the mitochondrial eytochrome oxidase submit (COI) gene region (450 bp) to investigate the genetic structure of the oriental river prawn ( Macrobrachium nipponense ) ...This study analyzed nueleotide sequences from the mitochondrial eytochrome oxidase submit (COI) gene region (450 bp) to investigate the genetic structure of the oriental river prawn ( Macrobrachium nipponense ) among nine populations from the Yangtze and Lancang Rivers. A total of 79 individuals were collected for this work. Eighty-nine nucleotides were found to be variable, resulting in 46 haplotypes. Among the nine populations, the population from Kunming shows the greatest level of variability (h = 1.000, π = 0.028), whereas the population from Cbongqing exhibits the lowest level of variability (h = 0.700,π = 0.008). Analysis of molecular variance suggested that of the total genetic diversity, 9.66% was attributable to inter-population diversity and the remainder (90.34%) to differences within populations. A molecular phylogenetic tree constructed using the Neighbor-joining (N J) method showed that the 46 haplotypes were assigned to two clades associated with geographic regions. These results provide basic information for the conservation and sustainable exploitation of this species.展开更多
The Yangtze River estuary is the main production area of Anguilla japonica in China,as well as the only existing fishery area for adult eels.Japanese eels are distributed in the main rivers and many tributaries from t...The Yangtze River estuary is the main production area of Anguilla japonica in China,as well as the only existing fishery area for adult eels.Japanese eels are distributed in the main rivers and many tributaries from the Yangtze River estuary to the upper Jinsha River,which extend to nearly 3 000 km.However,their migration behaviors remain relatively unknown.We analyzed the biological characteristics of 153 specimens of silver eels collected from the Jingjiang section of Yangtze River(31o30'N,120o42'E) between September and November,2008,and tested the sagittal Sr/Ca ratios of 27 specimens.Among the 153 specimens examined,85 were female and 68 were male,which translated to a female-male ratio of 1:0.8.The ages of the female specimens ranged from 3 to 7 a(average 5.52) with an average total length(TL) of(669±80) mm,average body weight(BW) of(555±229)g,average condition factor of 1.77±0.22,and average gonad somatic index(GSI) of 1.32±0.31.The ages of the males ranged from 3 to 5 a(average:4.38) with an average TL of(518±51) mm,average BW of(234±76) g,average condition factor of 1.62±0.18,and average GSI of 0.21±0.11.All biological parameters of females were significantly larger than those of the male specimens(P0.05).According to the average Sr/Ca ratio(7.99±1.05) ×10-3 of the elver mark of sagitta,17 individuals(62.96%) were river eels and 10 individuals(37.04%) were estuarine eels.Of 16 females,13 individuals(81.25%) were river eels and 3 were estuarine eels,while of 11 males,36.36% were river eels and 63.64% were estuarine eels.The analysis on Sr/Ca ratios for every growth layer group(GLG) indicated there were no significant differences between second-age males and females.However,significant differences were observed between the third-age,fourth-age,and migration-age male and female specimens.This was likely related to the fact that second-age eels of both sexes stay in the same inhabitation waters;however,as they grow older,they move to different areas.展开更多
Amphioxus has an important evolutionary position as a result of their phylogenetic position relative to vertebrates. Understanding their chromosomes would provide key points in the study of evolutionary biology and co...Amphioxus has an important evolutionary position as a result of their phylogenetic position relative to vertebrates. Understanding their chromosomes would provide key points in the study of evolutionary biology and comparative genomics. The difficulty in preparing amphioxus chromosomes currently provides a significant hurdle in this research. In the current study, we describe an improved method for metaphase preparation from amphioxus embryos and methodology for preparing metaphase spreads from regenerative somatic cells. Chromosomes of two amphioxus species from Xiamen waters in China are also observed. The diploid chromosome number was found to be 40 in Branchiostoma belcheri, while B. japonicum has 36, confirming the two are distinct species from cytotaxonomic viewpoint.展开更多
Crustal deformation can provide constraints for studying earthquake rupture and shock wave transmission for the Mw9.0 eastern Japan great earthquake. Using the single- epoch precise point positioning (PPP) method an...Crustal deformation can provide constraints for studying earthquake rupture and shock wave transmission for the Mw9.0 eastern Japan great earthquake. Using the single- epoch precise point positioning (PPP) method and the appropriate positioning flow, we process GPS data from six IGS (International GNSS Service) sites (e.g., MIZU, TSK2, USUD, MTKA, AIRA and KSMV) located in Japan and obtain the positioning results with centimeter scale precision. The displacement time series of the six sites are analyzed using the least squares spectral analysis method to estimate deformations caused by the Mw9.0 mainshock and the Mw7.9 aftershock, and the cumulative displacements after 1 day. Mainshock displacements at station MIZU, the nearest site to the mainshock in the North (N), East (E), and Up (U) directions, are -1.202 m, 2.180 m and -0.104 m, respectively, and the cumulative deformations after 1 day are -1.117 m, 2.071 m and -0.072 m, respectively. The displacements at station KSMV, the nearest site to the Mw7.9 aftershock in the N, E and U directions, are -0.032 m, 0.742 m and -0.345 m, respectively. The other sites obviously experienced eastern movements and subsidence. The deformation vectors indicate that the horizontal displacements caused by the earthquake point to the epicenter and rupture. Elastic bounds evidently took place at all sites. The results indicate that the crustal movements and earthquake were part of a megathrust caused by the Pacific Plate sinking under the North American Plate to the northeast of Japan island arc.展开更多
[Objective] The study aimed to establish an approach to high titer Et immune serum preparation by low-aged Japanese rabbits. [Method] Antigen of Et was prepared at first, Japanese rabbits were taken as immunized anima...[Objective] The study aimed to establish an approach to high titer Et immune serum preparation by low-aged Japanese rabbits. [Method] Antigen of Et was prepared at first, Japanese rabbits were taken as immunized animal, and divided into two groups for experiments, one is two-month-old group (T group), and another is six-month-old group (S group). Japanese rabbits were continuous by immunized with low-dose by using auricular intravenous method, then immune sera were collected. Immune serum antibody titer was determined with micro-agglutination reaction method. E Resultl Agglutination reaction showed that the Et serum titer of S group is higher than that of T group in the first testing with the same dose. But in the second testing, the serum titer of the T group and and the S group was consistent, [Conclusien] The animals in the T group (Japanese rabbits) were fewer months old, and produced high titer antiserum was which consistent with the S group, which indicated that the method of preparing of high titer Et immune serum by low-aged Japanese rabbits was feasible.展开更多
[Objective] The aim was to explore the composition of essential oils of Morus alba leaves and Chamaecyparis pisifera outer heartwood. [Method] The essential oils of M. alba leaves and C. pisifera outer heartwood were ...[Objective] The aim was to explore the composition of essential oils of Morus alba leaves and Chamaecyparis pisifera outer heartwood. [Method] The essential oils of M. alba leaves and C. pisifera outer heartwood were analyzed by gas chromatography-mass spectrometry,respectively. [Result] Alcohols [phytol (59.47%,extracted 3 h for M. alba leaves),α-cadinol (38.77%,extracted 6 h for C. pisifera outer heartwood)] were their first principal volatile components. Phytol was usually used as pharmaceutical raw material and α-cadinol was a kind of active organic compound with antibacterial function. [Conclusion] The essential oils of M. alba leaves and C. pisifera outer heartwood with hydrodistillation have medicinal value.展开更多
Structure, species composition, and soil properties of a subtropical evergreen broad-leaved forest in Okinawa, Japan, were examined by establishment of plots at thirty sites. The forest was characterized by a relative...Structure, species composition, and soil properties of a subtropical evergreen broad-leaved forest in Okinawa, Japan, were examined by establishment of plots at thirty sites. The forest was characterized by a relatively low canopy and a large number of small-diameter trees. Mean canopy height for this forest was 10 m and stands contained an average of 5400 stems-ha^-1 ( -〉 3.0 cm DBH); 64% of those stems were smaller than 10 cm DBH. The total basal area was 54.4 m^2-ha^-1, of which Castanopsis sieboldii contributed 48%. The forest showed high species diversity of trees. 80 tree species (≥ 3.0 cm DBH) from 31 families was identified in the thirty sampling plots. C. sieboldii and Schima wallichii were the dominant and subdominant species in terms of importance value. The mean tree species diversity indices for the plots were, 3.36 for Diversity index (H'), 0.71 for Equitability index (J') and 4.72 for Species richness index (S'), all of which strongly declined with the increase of importance value of the dominant, C. sieboldii. Measures of soil nutrients indicated low fertility, extreme heterogeneity and possible A1 toxicity. Regression analysis showed that stem density and the dominant tree height were significantly correlated with soil pH. There was a significant positive relationship between species diversity index and soil exchangeable K^+, Ca^2+, and Ca^2+/Al^3- ratio (all p values 〈0.001) and a negative relationship with N, C and P. The results suggest that soil property is a major factor influencing forest composition and structure within the subtropical forest in Okinawa.展开更多
The expressed sequence tag of eukaryotic translation initiation factor 5 (eIF5) from the Schistosoma japonicum adult worm cDNA library through subtractive hybridization between male and female worms was analyzed by ...The expressed sequence tag of eukaryotic translation initiation factor 5 (eIF5) from the Schistosoma japonicum adult worm cDNA library through subtractive hybridization between male and female worms was analyzed by the bioinformatics method. The overlapping sequences were assembled into one that includes the complete open reading frame (GenBank accession number: AY686501). The full-length cDNA of SjeIF5 was cloned into a pET-28c^(+) vector, which generated a prokaryotic expression plasmid, and a fusion protein of 18 kDa was induced in Escherichia coll. The recombinant expression of eIF5 protein of Schistosoma japonicum was purified. The immunoprotection test against schistosomiasis demonstrated that the recombinant protein worked to a certain extent, especially in the reduction of eggs in the liver of the host.展开更多
[Objective] The aim is to investigate the impact of different host plants, planting methods and directions on the damage law of Asterococcus muratae. [Method] The quantity of pests, size of female pests and quantity o...[Objective] The aim is to investigate the impact of different host plants, planting methods and directions on the damage law of Asterococcus muratae. [Method] The quantity of pests, size of female pests and quantity of ova of A. mu-ratae in Suzhou in different host plants under two planting methods and in four di-rections of a host plant were measured. [Result] A. muratae exhibits selectivity to host plants, and their quantity, female pest size and reproductive capacity are higher in Magnolia grandiflora(southern magnolia) than in other plants. The four directions of the host plant suffer to different extents, and the north part is usual y the most in-jured. Mass-planted southern magnolias are more seriously impacted than individual y-planted ones. Both size and weight of a female pest have significant linear relations with ovum quantity, and the weight of a female pest is more closely related to its ovum quantity. Based on the weight of a female pest, we can predict the exact ovum quantity according to the equation y=2E-05x+0.003 7. [Conclusion] The research re-sults provide reference for making an effective control plan for A. muratae.展开更多
AIM: To discuss the efficacy of endoscopic mass screening for gastric cancer.METHODS: The data used in this study were the results of mass screening programs for gastric cancer in Niigata City from 2002 to 2004. The...AIM: To discuss the efficacy of endoscopic mass screening for gastric cancer.METHODS: The data used in this study were the results of mass screening programs for gastric cancer in Niigata City from 2002 to 2004. The number of participants was 35089 in 2002, 34557 in 2003 and 36600 in 2004. The finding ratio referred to the final diagnosis of gastric cancer after a double check of endoscopic files and histological findings. The costs of identifying one case of gastric cancer were calculated based on the total expense for each screening program and additional close examinations. RESULTS: From the analysis of individual screening program with endoscopy, individual screening program with X-ray (ISX) and mass screening program with photofluorography (MSP) in reference to the finding ratio of gastric cancer, endoscopic examination was the best for detecting early gastric cancer, the finding ratio was 0.87% in 2004, approximately 2.7 and 4.6 times higher than those of the ISX and MSP groups. In addition, this novel method was the cheapest means regarding the cost of identifying one case of gastric cancer, which was estimated to be 1608000 Japanese yen in 2004. CONCLUSION: Endoscopic mass screening is a promising method and can be effectively applied if a sufficient number of skilled endoscopists become available to staff the system and if city offices support it.展开更多
Developing high-yielding rice(Oryza sativa L.)cultivars depends on having a better understanding of nitrogen(N) accumulation and translocation to the ear during the reproductive stage.Field experiments were carried ou...Developing high-yielding rice(Oryza sativa L.)cultivars depends on having a better understanding of nitrogen(N) accumulation and translocation to the ear during the reproductive stage.Field experiments were carried out to evaluate the genetic variation for N accumulation and translocation in different Japonica rice cultivars at different N rates and to identify any relationship to grain yield in southeast China.Four Japonica cultivars with similar agronomic characteristics were grown at two experimental sites in 2004 with three N rates of 0,60,and 180 kg N ha^(-1).Dry weights and N contents of rice plants were measured at tillering,initiation,anthesis,and maturity.Grain yields exhibited significant differences (P<0.05)among the cultivars and N application rates.Increasing N rates improved N uptake at anthesis and maturity in all four cultivars(P<0.05).N translocation from vegetative organs to the grains increased with enhanced N rates (P<0.05).N translocation to the grains ranged from 9 to 64 kg N ha^(-1)and N-translocation efficiency from 33% to 68%. Grain yield was linear to N uptake at anthesis(r^2=0.78^(**))and N translocation(r^2=0.67^(**)).Thus,cultivars with a high N uptake at anthesis,low residual N in the straw at maturity,and appropriate low N fertilizer supply in southeast China should efficiently increase N-recovery rate while maintaining grain yield and soil fertility.展开更多
基金JSPS KAKENHI Grant Numbers 18H03596(PI:Yosuke Kaifu)JP19H01336(PI:Hiroyuki Sato)21H00608(PI:Kazuki Morisaki)。
文摘The beginning of the Japanese Upper Paleolithic has mainly been examined using two major models:the Middle Paleolithic evolutionary model within the archipelago and the continental Upper Paleolithic diffusion/migration model.However,recent archeological data from Japan and nearby countries are challenging such simple models.This paper critically reviews previous chronology of the Japanese Paleolithic,including possible Lower and Middle Paleolithic(LP/MP),and attempts to show an alternative model of the beginning of the Japanese Upper Paleolithic.This paper suggests several possible specimens of LP/MP and recommends further geoarchaeological investigation to understand the reliability and cultural relationship between possible LP/MP specimens and the Early Upper Paleolithic(EUP).The start of the Japanese EUP is presently characterized by a flake industry with trapezoids and denticulates around 39-37 kaBP cal on Paleo-Honshu Island,which has partial resemblance with contemporary assemblages in China and the Korean Peninsula,although trapezoids are endemic only to the Japanese EUP and may have derived from the ancestral lithic tradition.Blade technology appeared earliest on Central Paleo-Honshu Island,about 1000 years later than the earliest flake technology.Although blade technology may have originated from the elongated flake technology of the previous period,the sudden simultaneous emergence implies that it diffused from the Korean Peninsula.This paper proposes that blade technology from the Korean Peninsula arrived on the northeastern Paleo-Honshu Island,including the Japan Sea coastal region of western Honshu,rather than the southwest,where flake technology long prospered,due to differences in ecological settings and adaptation strategies between the two regions.
基金This project was supported partly by Key Research Programof Zhejiang(2005C12006-01,2006C12005)
文摘This study analyzed nueleotide sequences from the mitochondrial eytochrome oxidase submit (COI) gene region (450 bp) to investigate the genetic structure of the oriental river prawn ( Macrobrachium nipponense ) among nine populations from the Yangtze and Lancang Rivers. A total of 79 individuals were collected for this work. Eighty-nine nucleotides were found to be variable, resulting in 46 haplotypes. Among the nine populations, the population from Kunming shows the greatest level of variability (h = 1.000, π = 0.028), whereas the population from Cbongqing exhibits the lowest level of variability (h = 0.700,π = 0.008). Analysis of molecular variance suggested that of the total genetic diversity, 9.66% was attributable to inter-population diversity and the remainder (90.34%) to differences within populations. A molecular phylogenetic tree constructed using the Neighbor-joining (N J) method showed that the 46 haplotypes were assigned to two clades associated with geographic regions. These results provide basic information for the conservation and sustainable exploitation of this species.
基金supported by the Chinese National Science Foundation under the Grant(30771650)the Key Programs of Science and Technology Commission Foundation of Shanghai(08391910200)+3 种基金Special Fund for Agroscientific Research in the Public Interest(201203065)Research Fund for the Doctoral Program of Higher Education of China(20070264001)E-class Programs of Shanghai Municipal Education Commission(E03009)the Key Subject Construction of Shanghai(S30701)
文摘The Yangtze River estuary is the main production area of Anguilla japonica in China,as well as the only existing fishery area for adult eels.Japanese eels are distributed in the main rivers and many tributaries from the Yangtze River estuary to the upper Jinsha River,which extend to nearly 3 000 km.However,their migration behaviors remain relatively unknown.We analyzed the biological characteristics of 153 specimens of silver eels collected from the Jingjiang section of Yangtze River(31o30'N,120o42'E) between September and November,2008,and tested the sagittal Sr/Ca ratios of 27 specimens.Among the 153 specimens examined,85 were female and 68 were male,which translated to a female-male ratio of 1:0.8.The ages of the female specimens ranged from 3 to 7 a(average 5.52) with an average total length(TL) of(669±80) mm,average body weight(BW) of(555±229)g,average condition factor of 1.77±0.22,and average gonad somatic index(GSI) of 1.32±0.31.The ages of the males ranged from 3 to 5 a(average:4.38) with an average TL of(518±51) mm,average BW of(234±76) g,average condition factor of 1.62±0.18,and average GSI of 0.21±0.11.All biological parameters of females were significantly larger than those of the male specimens(P0.05).According to the average Sr/Ca ratio(7.99±1.05) ×10-3 of the elver mark of sagitta,17 individuals(62.96%) were river eels and 10 individuals(37.04%) were estuarine eels.Of 16 females,13 individuals(81.25%) were river eels and 3 were estuarine eels,while of 11 males,36.36% were river eels and 63.64% were estuarine eels.The analysis on Sr/Ca ratios for every growth layer group(GLG) indicated there were no significant differences between second-age males and females.However,significant differences were observed between the third-age,fourth-age,and migration-age male and female specimens.This was likely related to the fact that second-age eels of both sexes stay in the same inhabitation waters;however,as they grow older,they move to different areas.
基金Supported by grants from NSFC (No.30570208)SRFDP of Ministry of Education, China (20070384041)The Education Department of Fujian Province, China (JB07063)
文摘Amphioxus has an important evolutionary position as a result of their phylogenetic position relative to vertebrates. Understanding their chromosomes would provide key points in the study of evolutionary biology and comparative genomics. The difficulty in preparing amphioxus chromosomes currently provides a significant hurdle in this research. In the current study, we describe an improved method for metaphase preparation from amphioxus embryos and methodology for preparing metaphase spreads from regenerative somatic cells. Chromosomes of two amphioxus species from Xiamen waters in China are also observed. The diploid chromosome number was found to be 40 in Branchiostoma belcheri, while B. japonicum has 36, confirming the two are distinct species from cytotaxonomic viewpoint.
基金supported partially by the National Natural Science Foundation of China(No.40974004 and 40974016)the Key Laboratory of Surveying and Mapping Technology on Island and Reef of NASMG,China(No.2011A01)the Key Laboratory of Advanced Surveying Engineering of NASMG,China(No.TJES1101)
文摘Crustal deformation can provide constraints for studying earthquake rupture and shock wave transmission for the Mw9.0 eastern Japan great earthquake. Using the single- epoch precise point positioning (PPP) method and the appropriate positioning flow, we process GPS data from six IGS (International GNSS Service) sites (e.g., MIZU, TSK2, USUD, MTKA, AIRA and KSMV) located in Japan and obtain the positioning results with centimeter scale precision. The displacement time series of the six sites are analyzed using the least squares spectral analysis method to estimate deformations caused by the Mw9.0 mainshock and the Mw7.9 aftershock, and the cumulative displacements after 1 day. Mainshock displacements at station MIZU, the nearest site to the mainshock in the North (N), East (E), and Up (U) directions, are -1.202 m, 2.180 m and -0.104 m, respectively, and the cumulative deformations after 1 day are -1.117 m, 2.071 m and -0.072 m, respectively. The displacements at station KSMV, the nearest site to the Mw7.9 aftershock in the N, E and U directions, are -0.032 m, 0.742 m and -0.345 m, respectively. The other sites obviously experienced eastern movements and subsidence. The deformation vectors indicate that the horizontal displacements caused by the earthquake point to the epicenter and rupture. Elastic bounds evidently took place at all sites. The results indicate that the crustal movements and earthquake were part of a megathrust caused by the Pacific Plate sinking under the North American Plate to the northeast of Japan island arc.
基金Supported by Science and Technology Key Project of Science and Technology Bureau of Tangshan:Study of Rapid Detection and Diag-nosis Technology on Key Aquatic Animal Pathogen(06125401A-3)~~
文摘[Objective] The study aimed to establish an approach to high titer Et immune serum preparation by low-aged Japanese rabbits. [Method] Antigen of Et was prepared at first, Japanese rabbits were taken as immunized animal, and divided into two groups for experiments, one is two-month-old group (T group), and another is six-month-old group (S group). Japanese rabbits were continuous by immunized with low-dose by using auricular intravenous method, then immune sera were collected. Immune serum antibody titer was determined with micro-agglutination reaction method. E Resultl Agglutination reaction showed that the Et serum titer of S group is higher than that of T group in the first testing with the same dose. But in the second testing, the serum titer of the T group and and the S group was consistent, [Conclusien] The animals in the T group (Japanese rabbits) were fewer months old, and produced high titer antiserum was which consistent with the S group, which indicated that the method of preparing of high titer Et immune serum by low-aged Japanese rabbits was feasible.
基金Supported by the Forestry Department of Heilongjiang Province Science and Technology Promotion Project (01043208003)Students Innovative Pilot Project (10102250107)~~
文摘[Objective] The aim was to explore the composition of essential oils of Morus alba leaves and Chamaecyparis pisifera outer heartwood. [Method] The essential oils of M. alba leaves and C. pisifera outer heartwood were analyzed by gas chromatography-mass spectrometry,respectively. [Result] Alcohols [phytol (59.47%,extracted 3 h for M. alba leaves),α-cadinol (38.77%,extracted 6 h for C. pisifera outer heartwood)] were their first principal volatile components. Phytol was usually used as pharmaceutical raw material and α-cadinol was a kind of active organic compound with antibacterial function. [Conclusion] The essential oils of M. alba leaves and C. pisifera outer heartwood with hydrodistillation have medicinal value.
基金supported by National Natural Science Foundation of China (No.30471386)Japanese Society for Promotion of Sciences (15P03118)
文摘Structure, species composition, and soil properties of a subtropical evergreen broad-leaved forest in Okinawa, Japan, were examined by establishment of plots at thirty sites. The forest was characterized by a relatively low canopy and a large number of small-diameter trees. Mean canopy height for this forest was 10 m and stands contained an average of 5400 stems-ha^-1 ( -〉 3.0 cm DBH); 64% of those stems were smaller than 10 cm DBH. The total basal area was 54.4 m^2-ha^-1, of which Castanopsis sieboldii contributed 48%. The forest showed high species diversity of trees. 80 tree species (≥ 3.0 cm DBH) from 31 families was identified in the thirty sampling plots. C. sieboldii and Schima wallichii were the dominant and subdominant species in terms of importance value. The mean tree species diversity indices for the plots were, 3.36 for Diversity index (H'), 0.71 for Equitability index (J') and 4.72 for Species richness index (S'), all of which strongly declined with the increase of importance value of the dominant, C. sieboldii. Measures of soil nutrients indicated low fertility, extreme heterogeneity and possible A1 toxicity. Regression analysis showed that stem density and the dominant tree height were significantly correlated with soil pH. There was a significant positive relationship between species diversity index and soil exchangeable K^+, Ca^2+, and Ca^2+/Al^3- ratio (all p values 〈0.001) and a negative relationship with N, C and P. The results suggest that soil property is a major factor influencing forest composition and structure within the subtropical forest in Okinawa.
文摘The expressed sequence tag of eukaryotic translation initiation factor 5 (eIF5) from the Schistosoma japonicum adult worm cDNA library through subtractive hybridization between male and female worms was analyzed by the bioinformatics method. The overlapping sequences were assembled into one that includes the complete open reading frame (GenBank accession number: AY686501). The full-length cDNA of SjeIF5 was cloned into a pET-28c^(+) vector, which generated a prokaryotic expression plasmid, and a fusion protein of 18 kDa was induced in Escherichia coll. The recombinant expression of eIF5 protein of Schistosoma japonicum was purified. The immunoprotection test against schistosomiasis demonstrated that the recombinant protein worked to a certain extent, especially in the reduction of eggs in the liver of the host.
文摘[Objective] The aim is to investigate the impact of different host plants, planting methods and directions on the damage law of Asterococcus muratae. [Method] The quantity of pests, size of female pests and quantity of ova of A. mu-ratae in Suzhou in different host plants under two planting methods and in four di-rections of a host plant were measured. [Result] A. muratae exhibits selectivity to host plants, and their quantity, female pest size and reproductive capacity are higher in Magnolia grandiflora(southern magnolia) than in other plants. The four directions of the host plant suffer to different extents, and the north part is usual y the most in-jured. Mass-planted southern magnolias are more seriously impacted than individual y-planted ones. Both size and weight of a female pest have significant linear relations with ovum quantity, and the weight of a female pest is more closely related to its ovum quantity. Based on the weight of a female pest, we can predict the exact ovum quantity according to the equation y=2E-05x+0.003 7. [Conclusion] The research re-sults provide reference for making an effective control plan for A. muratae.
文摘AIM: To discuss the efficacy of endoscopic mass screening for gastric cancer.METHODS: The data used in this study were the results of mass screening programs for gastric cancer in Niigata City from 2002 to 2004. The number of participants was 35089 in 2002, 34557 in 2003 and 36600 in 2004. The finding ratio referred to the final diagnosis of gastric cancer after a double check of endoscopic files and histological findings. The costs of identifying one case of gastric cancer were calculated based on the total expense for each screening program and additional close examinations. RESULTS: From the analysis of individual screening program with endoscopy, individual screening program with X-ray (ISX) and mass screening program with photofluorography (MSP) in reference to the finding ratio of gastric cancer, endoscopic examination was the best for detecting early gastric cancer, the finding ratio was 0.87% in 2004, approximately 2.7 and 4.6 times higher than those of the ISX and MSP groups. In addition, this novel method was the cheapest means regarding the cost of identifying one case of gastric cancer, which was estimated to be 1608000 Japanese yen in 2004. CONCLUSION: Endoscopic mass screening is a promising method and can be effectively applied if a sufficient number of skilled endoscopists become available to staff the system and if city offices support it.
基金Project supported by the National Natural Science Foundation of China(Nos.30390082 and 3027079)the Science and Technology Committee of Jiangsu Province,China(No.BE2005369).
文摘Developing high-yielding rice(Oryza sativa L.)cultivars depends on having a better understanding of nitrogen(N) accumulation and translocation to the ear during the reproductive stage.Field experiments were carried out to evaluate the genetic variation for N accumulation and translocation in different Japonica rice cultivars at different N rates and to identify any relationship to grain yield in southeast China.Four Japonica cultivars with similar agronomic characteristics were grown at two experimental sites in 2004 with three N rates of 0,60,and 180 kg N ha^(-1).Dry weights and N contents of rice plants were measured at tillering,initiation,anthesis,and maturity.Grain yields exhibited significant differences (P<0.05)among the cultivars and N application rates.Increasing N rates improved N uptake at anthesis and maturity in all four cultivars(P<0.05).N translocation from vegetative organs to the grains increased with enhanced N rates (P<0.05).N translocation to the grains ranged from 9 to 64 kg N ha^(-1)and N-translocation efficiency from 33% to 68%. Grain yield was linear to N uptake at anthesis(r^2=0.78^(**))and N translocation(r^2=0.67^(**)).Thus,cultivars with a high N uptake at anthesis,low residual N in the straw at maturity,and appropriate low N fertilizer supply in southeast China should efficiently increase N-recovery rate while maintaining grain yield and soil fertility.