To investigate the role of CD4 + helper T (Th) cells in the memory CTL-mediated anti-tumor immunity, the RAG-1 gene knock out mice were adoptively transferred with OT-1 cells to generate the memory CTL, the C57BL/6 mi...To investigate the role of CD4 + helper T (Th) cells in the memory CTL-mediated anti-tumor immunity, the RAG-1 gene knock out mice were adoptively transferred with OT-1 cells to generate the memory CTL, the C57BL/6 mice immunized with the epitope peptide of OVA specific Th cells and with different adjuvants were adoptively transferred with these memory-CTLs, and then the animals were challenged with tumor cells EG7. It was found that although the simple immunization of mice with the epitope peptide of the OVA specific Th cells could generate more effect CTL, but this effect was not so strong enough to resist completely the challenges with tumor cells. Nevertheless, the memory CTL-mediated anti-tumor immune effect required the helps of Th1 and Th2 cells. The cross-regulation between Th1 and Th2 cells seemed to be beneficial for the host to generate more effector CTL for mounting an efficient anti-tumor response. It concluded that the interaction between Th1 and Th2 cells might be more important than the single subset of Th cells in the memory CTL-mediated anti-tumor immune response. More attention should be paid in this regard for the future studies.展开更多
Objective： To investigate the effects of resuscitation with normal saline（NS）, hypertonic saline（HTS）, and hydroxyethyl starch（HES） on regulatory T cells（Tregs）, helper T 1（Th1）/Th2 and cytotoxic T 1（Tc1）...Objective： To investigate the effects of resuscitation with normal saline（NS）, hypertonic saline（HTS）, and hydroxyethyl starch（HES） on regulatory T cells（Tregs）, helper T 1（Th1）/Th2 and cytotoxic T 1（Tc1）/Tc2 profiles in the treatment of hemorrhagic shock. Methods： Rats subjected to severe hemorrhagic shock were resuscitated for 30 min with NS（n=8）, HTS（n=8）, or HES（n=8）; sham（n=8） and naive control（n=8） groups were used for comparison. Following fluid resuscitation, the whole shed blood was reinfused for 30 min, and the rats were observed with continuous hemodynamic monitoring for 120 min. CD4^＋CD25^＋Foxp3^＋ Treg proportions, Th1/Th2 and Tc1/Tc2 profiles in spleen were analyzed by three-color flow cytometry. Results： The proportion of CD4^＋CD25^＋Foxp3^＋ Tregs and ratios of Th1/Th2 and Tc1/Tc2 did not differ among control, sham, and HTS groups, but were significantly lower in NS and HES groups（both P0.05 vs. sham）; NS and HES levels were similar. The level of Tc1 was significantly increased in HTS（P0.05 vs. sham）, and levels of Tc2 were increased in NS, HES, and HTS groups compared to sham（all P0.05）, but did not differ from each other. Conclusions： HTS resuscitation has a greater impact on immune system recovery than NS or HES by preserving the proportion of Tregs and maintaining the balance between Th1/Th2 and Tc1/Tc2 cells in the spleen. Thus, HTS resuscitation provides potential immunomodulatory activity in the early stage after hemorrhagic shock.展开更多
文摘To investigate the role of CD4 + helper T (Th) cells in the memory CTL-mediated anti-tumor immunity, the RAG-1 gene knock out mice were adoptively transferred with OT-1 cells to generate the memory CTL, the C57BL/6 mice immunized with the epitope peptide of OVA specific Th cells and with different adjuvants were adoptively transferred with these memory-CTLs, and then the animals were challenged with tumor cells EG7. It was found that although the simple immunization of mice with the epitope peptide of the OVA specific Th cells could generate more effect CTL, but this effect was not so strong enough to resist completely the challenges with tumor cells. Nevertheless, the memory CTL-mediated anti-tumor immune effect required the helps of Th1 and Th2 cells. The cross-regulation between Th1 and Th2 cells seemed to be beneficial for the host to generate more effector CTL for mounting an efficient anti-tumor response. It concluded that the interaction between Th1 and Th2 cells might be more important than the single subset of Th cells in the memory CTL-mediated anti-tumor immune response. More attention should be paid in this regard for the future studies.
基金supported by the National Natural Science Foundation of China(No.81272075)
文摘Objective： To investigate the effects of resuscitation with normal saline（NS）, hypertonic saline（HTS）, and hydroxyethyl starch（HES） on regulatory T cells（Tregs）, helper T 1（Th1）/Th2 and cytotoxic T 1（Tc1）/Tc2 profiles in the treatment of hemorrhagic shock. Methods： Rats subjected to severe hemorrhagic shock were resuscitated for 30 min with NS（n=8）, HTS（n=8）, or HES（n=8）; sham（n=8） and naive control（n=8） groups were used for comparison. Following fluid resuscitation, the whole shed blood was reinfused for 30 min, and the rats were observed with continuous hemodynamic monitoring for 120 min. CD4^＋CD25^＋Foxp3^＋ Treg proportions, Th1/Th2 and Tc1/Tc2 profiles in spleen were analyzed by three-color flow cytometry. Results： The proportion of CD4^＋CD25^＋Foxp3^＋ Tregs and ratios of Th1/Th2 and Tc1/Tc2 did not differ among control, sham, and HTS groups, but were significantly lower in NS and HES groups（both P0.05 vs. sham）; NS and HES levels were similar. The level of Tc1 was significantly increased in HTS（P0.05 vs. sham）, and levels of Tc2 were increased in NS, HES, and HTS groups compared to sham（all P0.05）, but did not differ from each other. Conclusions： HTS resuscitation has a greater impact on immune system recovery than NS or HES by preserving the proportion of Tregs and maintaining the balance between Th1/Th2 and Tc1/Tc2 cells in the spleen. Thus, HTS resuscitation provides potential immunomodulatory activity in the early stage after hemorrhagic shock.
