Background:Study the effect of Jiangu Shenjin decoction on the Wnt/β-catenin signalling pathway in knee osteoarthritis.Methods:Forty-eight female Sprague-Dawley rats were randomly divided into eight groups.After succ...Background:Study the effect of Jiangu Shenjin decoction on the Wnt/β-catenin signalling pathway in knee osteoarthritis.Methods:Forty-eight female Sprague-Dawley rats were randomly divided into eight groups.After successful modelling,the blank group and the model group were given a normal diet and drinking water,and the other rats were given meloxicam,an anti-bone hyperplasia agent and Jiangu Shenjin decoction by gavage for 4 weeks.The degree of movement and knee swelling were observed.Serum levels of interleukin-1β,interleukin-6,and tumour necrosis factor-αwere measured.The levels of inflammatory factors,pathological changes in articular cartilage and the mRNA expression levels ofβ-catenin and Wnt4 were examined.Results:Compared with the model group,the treatment group had lower levels than the model group.The medium-dose group had lower levels than the high-dose group.Compared with the meloxicam group and the anti-bone hyperplasia group,the medium-dose group had lower levels than the high-dose group.The medium-dose group had slightly lower levels than the other two groups,and the difference among the three groups was not significant.The mRNA expression level of Wnt4 andβ-catenin indicated thatβ-catenin was inhibited in the signalling pathway.The level ofβ-catenin was inhibited and then increased each treatment,and bone metabolism regulation by Jiangu Shenjin decoction was effective.Conclusion:Jiangu Shenjin decoction may act through Wnt to treat knee osteoarthritis.Theβ-catenin signalling pathway inhibits inflammatory factors and regulates proteins in the signalling pathway to affect bone metabolism.展开更多
Objective:To investigate the effects of Bushen Jiangu decoction (kidney invigorating and bone invigorating decoction) on sex hormones and cytokines in women with postmenopausal osteoporosis (PMOP).Methods:From Februar...Objective:To investigate the effects of Bushen Jiangu decoction (kidney invigorating and bone invigorating decoction) on sex hormones and cytokines in women with postmenopausal osteoporosis (PMOP).Methods:From February 2014 to May 2016, 98 PMOP patients were selected as the research objects, randomly divided into observation group and control group (n=49). Two groups were given oral alendronate 70 mg/ times, 1 time/week, Caltrate D 1 tablets, 2 times/d. The observation group was given Bushen Jiangu decoction, daily 1 agent. The treatment period was 6 months. The fasting peripheral venous blood was collected before and after treatment and analyzed by automated chemiluminescence immunoassay analyzer for determination of serum estradiol (E2), follicle stimulating hormone (FSH) and luteinizing hormone (LH), prolactin (PRL), testosterone (T) and type I collagen carboxy terminal peptide (CTX), type I precollagen (PINP);using radioimmunoassay method of interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-alpha), insulin-like growth factor (IGF-1).Results:The E2 and T levels of the observation group after treatment were significantly increased compared with before treatment (P<0.05). The IL-6 and TNF-alpha of the observation group after treatment were lower than before treatment, while IGF-1 increased than that before treatment, significantly better than the control group (P<0.05);The CTX, P of NP of the observation group after treatment was significantly lower than the control group during the same period (P<0.05).Conclusions:The combination of Bushen Jiangu decoction combined with sodium hyaluronate can effectively improve the level of sex hormones in patients with PMOP, regulate the level of IL-6, TNF-alpha and IGF-1, inhibit the formation of osteoclasts and bone resorption, and improve the treatment effect of osteoporosis.展开更多
目的探讨筋骨疗伤膏联合补肾接骨汤对老年下肢骨折患者术后肿胀及典型Wnt/β-链蛋白(Canonical Wnt/β-catenin pathway,Wnt/β-catenin)信号通路的影响。方法收集2021年1月—2022年7月在医院骨外科进行诊治的下肢骨折患者92例,随机数...目的探讨筋骨疗伤膏联合补肾接骨汤对老年下肢骨折患者术后肿胀及典型Wnt/β-链蛋白(Canonical Wnt/β-catenin pathway,Wnt/β-catenin)信号通路的影响。方法收集2021年1月—2022年7月在医院骨外科进行诊治的下肢骨折患者92例,随机数字法分为试验组(46例)及对照组(46例)。所有受试者予以切开复位内固定术,对照组术后接受常规康复手段和热水泡脚,试验组在对照组的基础上予以筋骨疗伤膏联合补肾接骨汤。对比两组临床恢复情况、肿胀程度、疼痛评分、血液流变学指标、Wnt/β-catenin信号通路血清蛋白水平、骨痂生长情况及不良反应。数据结果应用统计学软件SPSS 19.0加以处理。结果与对照组相比,试验组完全负重锻炼、骨折愈合时间缩短,骨折延迟愈合率较低(P<0.05)。与治疗前相比,两组治疗后肿胀程度、疼痛评分均明显降低(P<0.05),与对照组相比,试验组患者肿胀程度、疼痛评分较低(P<0.05)。与治疗前相比,两组治疗后β-catenin升高,β-Ⅰ型胶原羧基端肽(βisomer of C-terminal telopeptide of type Ⅰ collagen,β-CTX)及总Ⅰ型胶原氨基端延长肽(total type Ⅰ collagen amino terminal extender peptide,T-PINP)水平均明显降低(P<0.05),与对照组相比,试验组β-catenin水平较高,β-CTX及T-PINP水平较低(P<0.05)。与治疗前相比,两组骨痂量评分、骨痂密度评分均明显升高(P<0.05),与对照组相比,试验组治疗后骨痂量评分、骨痂密度评分较高(P<0.05)。与对照组相比,试验组并发症发生率较低(P<0.05)。结论通过筋骨疗伤膏联合补肾接骨汤可减轻老年下肢骨折患者的肿胀及疼痛程度,改善血液微循环,其治疗机制可能与调节Wnt/β-catenin信号通路具有密切相关性。展开更多
基金the General Project of the Natural Science Foundation of Hainan Province(No.819MS064).
文摘Background:Study the effect of Jiangu Shenjin decoction on the Wnt/β-catenin signalling pathway in knee osteoarthritis.Methods:Forty-eight female Sprague-Dawley rats were randomly divided into eight groups.After successful modelling,the blank group and the model group were given a normal diet and drinking water,and the other rats were given meloxicam,an anti-bone hyperplasia agent and Jiangu Shenjin decoction by gavage for 4 weeks.The degree of movement and knee swelling were observed.Serum levels of interleukin-1β,interleukin-6,and tumour necrosis factor-αwere measured.The levels of inflammatory factors,pathological changes in articular cartilage and the mRNA expression levels ofβ-catenin and Wnt4 were examined.Results:Compared with the model group,the treatment group had lower levels than the model group.The medium-dose group had lower levels than the high-dose group.Compared with the meloxicam group and the anti-bone hyperplasia group,the medium-dose group had lower levels than the high-dose group.The medium-dose group had slightly lower levels than the other two groups,and the difference among the three groups was not significant.The mRNA expression level of Wnt4 andβ-catenin indicated thatβ-catenin was inhibited in the signalling pathway.The level ofβ-catenin was inhibited and then increased each treatment,and bone metabolism regulation by Jiangu Shenjin decoction was effective.Conclusion:Jiangu Shenjin decoction may act through Wnt to treat knee osteoarthritis.Theβ-catenin signalling pathway inhibits inflammatory factors and regulates proteins in the signalling pathway to affect bone metabolism.
文摘Objective:To investigate the effects of Bushen Jiangu decoction (kidney invigorating and bone invigorating decoction) on sex hormones and cytokines in women with postmenopausal osteoporosis (PMOP).Methods:From February 2014 to May 2016, 98 PMOP patients were selected as the research objects, randomly divided into observation group and control group (n=49). Two groups were given oral alendronate 70 mg/ times, 1 time/week, Caltrate D 1 tablets, 2 times/d. The observation group was given Bushen Jiangu decoction, daily 1 agent. The treatment period was 6 months. The fasting peripheral venous blood was collected before and after treatment and analyzed by automated chemiluminescence immunoassay analyzer for determination of serum estradiol (E2), follicle stimulating hormone (FSH) and luteinizing hormone (LH), prolactin (PRL), testosterone (T) and type I collagen carboxy terminal peptide (CTX), type I precollagen (PINP);using radioimmunoassay method of interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-alpha), insulin-like growth factor (IGF-1).Results:The E2 and T levels of the observation group after treatment were significantly increased compared with before treatment (P<0.05). The IL-6 and TNF-alpha of the observation group after treatment were lower than before treatment, while IGF-1 increased than that before treatment, significantly better than the control group (P<0.05);The CTX, P of NP of the observation group after treatment was significantly lower than the control group during the same period (P<0.05).Conclusions:The combination of Bushen Jiangu decoction combined with sodium hyaluronate can effectively improve the level of sex hormones in patients with PMOP, regulate the level of IL-6, TNF-alpha and IGF-1, inhibit the formation of osteoclasts and bone resorption, and improve the treatment effect of osteoporosis.
文摘目的探讨筋骨疗伤膏联合补肾接骨汤对老年下肢骨折患者术后肿胀及典型Wnt/β-链蛋白(Canonical Wnt/β-catenin pathway,Wnt/β-catenin)信号通路的影响。方法收集2021年1月—2022年7月在医院骨外科进行诊治的下肢骨折患者92例,随机数字法分为试验组(46例)及对照组(46例)。所有受试者予以切开复位内固定术,对照组术后接受常规康复手段和热水泡脚,试验组在对照组的基础上予以筋骨疗伤膏联合补肾接骨汤。对比两组临床恢复情况、肿胀程度、疼痛评分、血液流变学指标、Wnt/β-catenin信号通路血清蛋白水平、骨痂生长情况及不良反应。数据结果应用统计学软件SPSS 19.0加以处理。结果与对照组相比,试验组完全负重锻炼、骨折愈合时间缩短,骨折延迟愈合率较低(P<0.05)。与治疗前相比,两组治疗后肿胀程度、疼痛评分均明显降低(P<0.05),与对照组相比,试验组患者肿胀程度、疼痛评分较低(P<0.05)。与治疗前相比,两组治疗后β-catenin升高,β-Ⅰ型胶原羧基端肽(βisomer of C-terminal telopeptide of type Ⅰ collagen,β-CTX)及总Ⅰ型胶原氨基端延长肽(total type Ⅰ collagen amino terminal extender peptide,T-PINP)水平均明显降低(P<0.05),与对照组相比,试验组β-catenin水平较高,β-CTX及T-PINP水平较低(P<0.05)。与治疗前相比,两组骨痂量评分、骨痂密度评分均明显升高(P<0.05),与对照组相比,试验组治疗后骨痂量评分、骨痂密度评分较高(P<0.05)。与对照组相比,试验组并发症发生率较低(P<0.05)。结论通过筋骨疗伤膏联合补肾接骨汤可减轻老年下肢骨折患者的肿胀及疼痛程度,改善血液微循环,其治疗机制可能与调节Wnt/β-catenin信号通路具有密切相关性。
