Radix achyranthis bidentatae(RAB), a member of the Amaranthaceae family, has been widely used in Traditional Chinese Medicine for1000 s of years. Increasing interest in RAB-derived medicinal has led to the discovery o...Radix achyranthis bidentatae(RAB), a member of the Amaranthaceae family, has been widely used in Traditional Chinese Medicine for1000 s of years. Increasing interest in RAB-derived medicinal has led to the discovery of additional triterpenoid saponins, phytoecdysones,polysaccharides, and many other compounds, as well as investigations into their pharmacology. A large number of pharmacological studies have shown RAB and its active components possess a range of pharmacological activities, including anti-tumor, anti-fertility, anti-senile, and anti?inflammatory effects. This review is an up?to?date summary and synthesis of the uses of RAB from phytochemical and pharmacological perspectives.展开更多
目的:基于网络药理学技术研究牛膝-钩藤药对治疗糖尿病合并高血压的作用机制。方法:基于中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)检索牛膝、钩藤的...目的:基于网络药理学技术研究牛膝-钩藤药对治疗糖尿病合并高血压的作用机制。方法:基于中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)检索牛膝、钩藤的活性成分及相关作用靶点,利用Cytoscape 3.7.2软件构建“药物-活性成分-靶点”相互作用网络图;在人类孟德尔遗传数据库(online mendelian inheritance in man,OMIM)、人类基因数据库(GeneCards)、DrugBank数据库及疗效药靶数据库(therapeutic target database,TTD)中筛选糖尿病、高血压的相关靶点,与活性成分靶点进行映射,并绘制“活性成分-疾病”交集靶点韦恩图。利用STRING在线分析平台分析交集靶点的相互作用关系,利用CentiScaPe 2.2插件进行网络拓扑分析,筛选出牛膝-钩藤治疗糖尿病合并高血压的潜在核心靶点。采用基因注释和资源分析数据库(Metascape)对潜在核心靶点进行基因本体(gene ontology,GO)功能富集分析和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,并利用Cytoscape 3.7.2软件构建“活性成分-核心靶点-通路-疾病”网络图。结果:共筛选出牛膝-钩藤活性成分46个及其作用靶点199个,糖尿病合并高血压相关靶点1 494个,将活性成分靶点与疾病靶点取交集,得到104个交集靶点。网络拓扑分析筛选出潜在核心靶点47个。GO富集分析得到生物过程1 004条、分子功能32条及细胞组成84条。KEGG通路富集分析得出153条信号通路。结论:牛膝-钩藤药对通过多成分、多靶点、多途径干预并治疗糖尿病合并高血压。展开更多
基金supported by the National Natural Science Foundation of China (grant number 81803690)the Innovative Talents Funding of Heilongjiang University of Chinese Medicine (grant number 2012RCQ20)+5 种基金the Heilongjiang Postdoctoral Innovation Foundation (grant number LBH-Z10020)the Heilongjiang University of Chinese Medicine Doctoral Innovation Foundation (grant number 2013bs04)the Natural Science Foundation of Heilongjiang Province (grant number H201324)the Specialized Research Fund for the Doctoral Program of Higher Education of China (grant number 20132327120003)the Heilongjiang Postdoctoral Scientific Research Developmental Fund (grant number LBH-Q16210)the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (grant number UNPYSCT-2017219)
文摘Radix achyranthis bidentatae(RAB), a member of the Amaranthaceae family, has been widely used in Traditional Chinese Medicine for1000 s of years. Increasing interest in RAB-derived medicinal has led to the discovery of additional triterpenoid saponins, phytoecdysones,polysaccharides, and many other compounds, as well as investigations into their pharmacology. A large number of pharmacological studies have shown RAB and its active components possess a range of pharmacological activities, including anti-tumor, anti-fertility, anti-senile, and anti?inflammatory effects. This review is an up?to?date summary and synthesis of the uses of RAB from phytochemical and pharmacological perspectives.
文摘目的用网络药理学方法研究牛膝防治慢性牙周炎(chronic periodontitis, CP)的主要活性成分,以及分析其潜在分子机制和药理活性。方法在TCMSP数据库获取牛膝的主要化学成分及作用靶点,从Genecards等数据库搜索CP疾病靶点,通过韦恩分析获得交集靶点。构建牛膝防治CP的"成分-靶点"网络,并利用STRING数据库构建蛋白互作网络(protein protein interaction network,PPI network)。进行基因本体(gene ontology, GO)和京都基因和基因组百科全书(kyoto encyclopedia of genes and genomes, KEGG)富集分析后,构建"成分-靶点-通路"网络。结果获得牛膝活性成分20个,交集靶点183个,其中关键靶点包括丝氨酸/苏氨酸蛋白激酶1(AKT1)、白细胞介素-6(IL-6)、肿瘤坏死因子(TNF)等。牛膝治疗CP的主要活性成分有槲皮素、黄芩素、黄芩苷等。GO富集分析得到抑菌活性等952个GO条目。KEGG富集分析得到IL-17信号通路等101条通路。结论阐述了牛膝中治疗CP的主要成分、作用靶点和途径,为进一步开发牛膝中的主要成分来防治CP具有重要的研究价值。
文摘目的:基于网络药理学技术研究牛膝-钩藤药对治疗糖尿病合并高血压的作用机制。方法:基于中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)检索牛膝、钩藤的活性成分及相关作用靶点,利用Cytoscape 3.7.2软件构建“药物-活性成分-靶点”相互作用网络图;在人类孟德尔遗传数据库(online mendelian inheritance in man,OMIM)、人类基因数据库(GeneCards)、DrugBank数据库及疗效药靶数据库(therapeutic target database,TTD)中筛选糖尿病、高血压的相关靶点,与活性成分靶点进行映射,并绘制“活性成分-疾病”交集靶点韦恩图。利用STRING在线分析平台分析交集靶点的相互作用关系,利用CentiScaPe 2.2插件进行网络拓扑分析,筛选出牛膝-钩藤治疗糖尿病合并高血压的潜在核心靶点。采用基因注释和资源分析数据库(Metascape)对潜在核心靶点进行基因本体(gene ontology,GO)功能富集分析和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,并利用Cytoscape 3.7.2软件构建“活性成分-核心靶点-通路-疾病”网络图。结果:共筛选出牛膝-钩藤活性成分46个及其作用靶点199个,糖尿病合并高血压相关靶点1 494个,将活性成分靶点与疾病靶点取交集,得到104个交集靶点。网络拓扑分析筛选出潜在核心靶点47个。GO富集分析得到生物过程1 004条、分子功能32条及细胞组成84条。KEGG通路富集分析得出153条信号通路。结论:牛膝-钩藤药对通过多成分、多靶点、多途径干预并治疗糖尿病合并高血压。
