Objective: To investigate changes of Ca2+ activated potassium channels (KCa) in autogenous vein grafts. Methods: Contraction of venous ring was measured by means of perfusion in vitro. The intimal rabbits proliferatio...Objective: To investigate changes of Ca2+ activated potassium channels (KCa) in autogenous vein grafts. Methods: Contraction of venous ring was measured by means of perfusion in vitro. The intimal rabbits proliferation of vascular and proliferation of cultured smooth muscle cells(vascular smooth muscle cells, VSMCs)were observed by the means of computerised image analysis and MTT method respectively. Furthermore, whole cell mode of patch clamp was used to record KCa of VSMCs isolated from autogenous vein grafts. Results: One week after transplantation there were no significant differences of contraction and intimal relative thickness between autogenous vein grafts and control. Contraction and intimal relative thickness of autogenous vein graft were significantly increased 2 weeks after transplantation (P<0.05, n=8 vs control), and they was more enhanced 4 weeks after vein transplantation (P<0.01, n=8 vs control).TEA(blocker of Ca2+ activated potassium channels)increased MTT A490 nm value of VSMCs from femoral vein in a dose dependent manner(P<0.05, n=8). KCa current density was significantly attenuated in VSMCs from autogenous vein grafts (1-4) week after transplantation(P<0.05, n=5).Conclusion: KCa is inhibited in autogenous vein graft, which account for vasospasm and intimal proliferation.展开更多
Objective: To evaluate the possibility of Salvia Miltiorrhiza (SM) on the prevention of restenosis, the authors investigated the effects of SM on the intimal thickening of air-injured carotid artery of rats, andexamin...Objective: To evaluate the possibility of Salvia Miltiorrhiza (SM) on the prevention of restenosis, the authors investigated the effects of SM on the intimal thickening of air-injured carotid artery of rats, andexamined the effects of SM on the proliferation of rabbit aortic median smooth muscle cells (SMC) cultivated invitro. Methods' Artery injury model of 17 rats about 4 months old was established by Fishman air-dry method.Fourteen days after operation, the maximal artery intimal and medial thickness of the control and SM groupwere measured on the image analysis system. Using cell counting and thymidine ('H-TdR) up-take method, theauthors also examined the effects of SM on the proliferation of aortic median SMC from 4 rabbits. Results: SMinhibited the proliferation and 3 H-TdR up-take of SMC in a dose-dependent manner in vitro (P < 0. 05 or 0. 01vs. control). It also inhibited the intimal thickening of rat arteries after deendothelialization. The maximal intimal thickness of the SM group was much thinner than that of the control (15. 22 1 2. 19 Urn vs. 80. 17 1 23. 49pm, P< 0. 01). Conclusions: The study raises the possibility that SM can be used to prevent clinical restenosis.展开更多
文摘Objective: To investigate changes of Ca2+ activated potassium channels (KCa) in autogenous vein grafts. Methods: Contraction of venous ring was measured by means of perfusion in vitro. The intimal rabbits proliferation of vascular and proliferation of cultured smooth muscle cells(vascular smooth muscle cells, VSMCs)were observed by the means of computerised image analysis and MTT method respectively. Furthermore, whole cell mode of patch clamp was used to record KCa of VSMCs isolated from autogenous vein grafts. Results: One week after transplantation there were no significant differences of contraction and intimal relative thickness between autogenous vein grafts and control. Contraction and intimal relative thickness of autogenous vein graft were significantly increased 2 weeks after transplantation (P<0.05, n=8 vs control), and they was more enhanced 4 weeks after vein transplantation (P<0.01, n=8 vs control).TEA(blocker of Ca2+ activated potassium channels)increased MTT A490 nm value of VSMCs from femoral vein in a dose dependent manner(P<0.05, n=8). KCa current density was significantly attenuated in VSMCs from autogenous vein grafts (1-4) week after transplantation(P<0.05, n=5).Conclusion: KCa is inhibited in autogenous vein graft, which account for vasospasm and intimal proliferation.
文摘Objective: To evaluate the possibility of Salvia Miltiorrhiza (SM) on the prevention of restenosis, the authors investigated the effects of SM on the intimal thickening of air-injured carotid artery of rats, andexamined the effects of SM on the proliferation of rabbit aortic median smooth muscle cells (SMC) cultivated invitro. Methods' Artery injury model of 17 rats about 4 months old was established by Fishman air-dry method.Fourteen days after operation, the maximal artery intimal and medial thickness of the control and SM groupwere measured on the image analysis system. Using cell counting and thymidine ('H-TdR) up-take method, theauthors also examined the effects of SM on the proliferation of aortic median SMC from 4 rabbits. Results: SMinhibited the proliferation and 3 H-TdR up-take of SMC in a dose-dependent manner in vitro (P < 0. 05 or 0. 01vs. control). It also inhibited the intimal thickening of rat arteries after deendothelialization. The maximal intimal thickness of the SM group was much thinner than that of the control (15. 22 1 2. 19 Urn vs. 80. 17 1 23. 49pm, P< 0. 01). Conclusions: The study raises the possibility that SM can be used to prevent clinical restenosis.
