Probes and nano-delivery systems targeting NAD(P)H:quinone oxidoreductase 1:a mini-review

The two-electron cytoplasmic reductase NAD(P)H:quinone oxidoreductase 1 is expressed in many tissues.NAD(P)H:quinone oxidoreductase 1 is well-known for being highly expressed in most cancers.Therefore,it could be a target for cancer therapy.Because it is a quinone reductase,many bioimaging probes based on quinone structures target NAD(P)H:quinone oxidoreductase 1 to diagnose tumours.Its expression is higher in tumours than in normal tissues,and using target drugs such asβ-lapachone to reduce side effects in normal tissues can help.However,the physicochemical properties ofβlapachone limit its application.The problem can be solved by using nanosystems to deliverβ-lapachone.This minireview summarizes quinone-based fluorescent,nearinfrared and two-photon fluorescent probes,as well as nanosystems for delivering the NAD(P)H:quinone oxidoreductase 1-activating drugβ-lapachone.This review provides valuable information for the future development of probes and nano-delivery systems that target NAD(P)H:quinone oxidoreductase 1.

茶叶防癌有效组分对NAD(P)H-醌还原酶的诱导作用

通过检测体外培养的ＨｅｐＧ２肝肿瘤细胞中ＮＡＤ（Ｐ）Ｈ－醌还原酶（ＱＲ）的活性，对茶叶中多种组分及单体诱导代谢解毒酶活性的能力进行了比较。结果发现，茶多酚、茶色素、混合茶对ＱＲ活性均有较明显的诱导作用；茶色素成分茶红素、茶多酚单体成分表没食子儿茶素没食子酸酯（ＥＧＣＧ）、表儿茶素没食子酸酯（ＥＣＧ）等也显示了一定的诱导作用，而表没食子儿茶素（ＥＧＣ）、表儿茶素（ＥＣ）、茶黄素、茶类黄醇、茶多糖和茶咖啡因的诱导作用不明显。结果表明：茶叶多种组分和单体均具有对体内解毒酶ＱＲ活性的诱导作用，而其中混合成份比单体成份的作用更为明显。因此，茶叶的抗氧化作用应是以茶多酚。

NQO1 C609T polymorphism correlated to colon cancer risk in farmers from western region of Inner Mongolia

Objective： To investigate the relationship between NAD（P）H：quinone oxidoreductase 1 （NQOI） C609T polymorphism and colon cancer risk in farmers from western region of Inner Mongolia. Methods： Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was performed to analyze NQO1 C609T polymorphism from 160 healthy controls and 76 colon cancer patients. Results： Among the colon cancer patients, the incidence of NQOI T allele （53.29%） was significantly higher than it in control group （33.75%, P〈0.001）. The individuals with NQO1 T allele had higher risk [2.239 （95% CI： 1.510-3.321） times] to develop colon cancer than individuals with NQO1 C allele. The incidence of NQO1 （TFI-） （34.21%） in colon cancer patients was higher than that in control group （15.62%, P〈0.001）. Odds ratios （OR） analysis suggested that NQO1 （T/F） and NQOI （T/C） genotype carriers had 3.813 （95% CI： 1.836-7.920） times and 2.080 （1.026-4.219） times risk compared with wild-type NQO1 （C/C） gene carriers in developing colon cancer. Individuals with NQO1 （T/I＇） genotype had 2.541 （95% CI： 0.990-6.552） times, 3.713 （95% CI： 1.542-8.935） times, and 3.471 （95% CI： 1.356-8.886） times risk than individuals with NQOI （T/C） or NQOI （C/C） genotype in well- differentiated, moderately-differentiated, and poorly-differentiated colon cancer patients, respectively. Conclusions： NQO1 gene C609T could be one of risk factors of colon cancer in farmers from western region of Inner Mongolia,

NQO1基因多态性与大肠癌遗传易感性

目的:探讨依赖还原型辅酶Ⅰ/Ⅱ:醌氧化还原酶(NQO1)基因多态性与大肠癌遗传易感性之间的相关性。方法:采用聚合酶链反应—限制性片段长度多态性(PCR-RFLP)基因型分析技术对101例大肠癌患者及103例对照者NQO1cDNA609位点多态性进行测定。结果:基因型频率分布在大肠癌组与对照组差异显著(χ2=9.52,P<0.01),T/T基因型携带者大肠癌患病危险性是野生纯合型(C/C)的3.56倍(OR=3.56,95%CI为1.58～7.96)。NQO1T等位基因及C等位基因频率在大肠癌组与对照组分别为42.1%、57.3%,57.9%、42.7%,T等位基因频率两组有显著差异(χ2=9.43,P<0.01),T等位基因携带者患大肠癌的危险性是C等位基因携带者的1.85倍,(OR=1.85,95%CI为1.25～2.73)。结论:NQO1在大肠癌的形成过程中起一定的保护作用,而NQO1cDNA609位点T等位基因可能是大肠癌发生的危险因素,NQO1基因的多态性与生活特征共同决定个体大肠癌的患病风险。

CD44、NQO1基因多态性与肺癌易感性及化疗预后的相关性研究

目的探究CD44、对醌氧化还原酶(NQO1)基因多态性与肺癌易感性及化疗预后的相关性。方法选取98例肺癌患者(肺癌组)和100例健康对照人群(对照组),选择CD44基因的rs8193和rs13347两个SNP位点,并选择NQO1基因rs1437135、rs10517和rs18005663个位点进行基因多态性检测,分析2组分布特征;分析CD44、NQO1基因多态性与肺癌患者临床病理特征及化疗疗效的关系。结果CD44基因的rs8193、rs13347基因位点及NQO1基因rs1437135、rs10517、rs1800566基因位点的基因型和分布频率在肺癌组及对照组比较差异有统计学意义(P<0.05)。对比化疗显效和无效肺癌患者性别、年龄、吸烟史、病理类型差异无统计学意义(P>0.05);化疗显效和无效肺癌患者CD44基因的rs8193、rs13347基因位点及NQO1基因rs1437135、rs10517、rs1800566基因位点多态性比较差异具有统计学意义(P<0.05)。不同CD44、NQO1基因型的肺癌患者中位生存时间(MST)比较差异具有统计学意义(P<0.05)。结论CD44、NQO1基因多态性与肺癌易感性、化疗效果及远期生存存在密切关系。CD44基因rs8193位点CC基因型、NQO1基因rs1437135位点AA基因型及rs1800566位点CC型可能为抑制肺癌发生的保护因素,CD44基因rs13347位点CC基因型、NQO1基因rs10517位点CC基因型为肺癌发生的风险因素。