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SDSP-AGE结合^(125)I示踪法与ELISA法测定血中rh-bFGF的比较 被引量:1
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作者 龙启才 刘长征 +1 位作者 符志莉 梁昌盛 《中国现代应用药学》 CAS CSCD 2000年第6期453-456,共4页
目的 :比较和评价SDS 聚丙烯酰胺凝胶电泳 (SDS PAGE)结合12 5I示踪法与ELISA法测定血浆中重组人碱性成纤维细胞生长因子 (recombinanthumanbasicfibroblastgrowthfactor,rh bFGF)。方法 :1 0只大鼠iv12 5I rh bFGF1 60 0ng/kg ,用SDS P... 目的 :比较和评价SDS 聚丙烯酰胺凝胶电泳 (SDS PAGE)结合12 5I示踪法与ELISA法测定血浆中重组人碱性成纤维细胞生长因子 (recombinanthumanbasicfibroblastgrowthfactor,rh bFGF)。方法 :1 0只大鼠iv12 5I rh bFGF1 60 0ng/kg ,用SDS PAGE结合放射性计数测定血浆rh bFGF含量。 9只大鼠ivrh bFGF 1 60 0ng/kg ,用ELISA法测定血浆rh bFGF含量。结果 :两种方法的灵敏度、回收率和重现性好。ELISA法测定血浆rh bFGF含量较SDS PAGE结合12 5I示踪法低。结论 :SDS PAGE结合12 展开更多
关键词 重组人碱性成纤维细胞生长因子(rhbFGF) 大鼠 ^^(125)I标记 SDS-聚丙烯酰胺凝胶电泳(DSD-PAGE) ELISA
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First application of plutonium in soil erosion research on terraces
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作者 Yong-Jing Guan Wu Chen +12 位作者 Shen-Zhen Wang Yu-Xin Hua Qiao-Yan Jing Zhi-Yong Liu Chun-Ping Huang De-Yu Wang Hui-Juan Wang Xian-Wen He Mario De Cesare Liang-Jia Cui Hua He Kai-Di Fan Zi-Chen Guo 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2023年第4期41-53,共13页
The spatial distributions of ^(239+240)Pu and ^(137)Cs in soils from Longji Rice Terraces were investigated to evaluate soil erosion.The activity concentrations of ^(239+240)Pu and ^(137)Cs in the surface soils of the... The spatial distributions of ^(239+240)Pu and ^(137)Cs in soils from Longji Rice Terraces were investigated to evaluate soil erosion.The activity concentrations of ^(239+240)Pu and ^(137)Cs in the surface soils of the paddy fields were in the range of 0.089–0.734 and1.80–7.88 mBq/g,respectively.The activities of ^(239+240)Pu and ^(137)Cs showed very similar distribution trends,first increasing and then decreasing with increasing elevation.The 240Pu/239Pu atom ratios in the surface soils ranged from 0.162 to 0.232.The activities of ^(239+240)Pu and ^(137)Cs in the soil cores tended to be uniformly distributed within the plowed layer and declined exponentially below this depth.The mean soil erosion rates of Longji Rice Terraces estimated by ^(239+240)Pu and ^(137)Cs tracer methods were 5.44 t/(ha·a)and 5.16 t/(ha·a),respectively,which demonstrated that plutonium can replace ^(137)Cs as an ideal tracer for soil erosion research in the future.Landform features are the main factors affecting the distribution of plutonium and ^(137)Cs as well as soil erosion in the Longji Rice Terraces. 展开更多
关键词 Soil erosion PLUTONIUM ^^(137)Cs tracer method TERRACES
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