^13C-UBT, histology and RUT were used in diagnosing HP infection in this study (gastric and duodenal ulcer, 65 cases; control, 36 cases). Any two tests of the above three are positive, then HP infection is confirmed. ...^13C-UBT, histology and RUT were used in diagnosing HP infection in this study (gastric and duodenal ulcer, 65 cases; control, 36 cases). Any two tests of the above three are positive, then HP infection is confirmed. Results: The positive rate of ^13C-UBT, histology and RUT are: 81.54%, 83.1%, 84.62%, sensitivity: 96.23%, 86.8%, 84.91%, specificity: 100%, 91.67%, 83.33%. We conclude that ^13C-UBT and histology and accurate in diagnosing HP infection; though the sensitivity and specificity of RUT are lower,展开更多
Background:The efficacy of the 13C-urea breath test(UBT)for diagnosis of Helicobacter pylori(H.pylori)infection in the remnant stomach after surgery is a matter of controversy.We report a simple and accurate method of...Background:The efficacy of the 13C-urea breath test(UBT)for diagnosis of Helicobacter pylori(H.pylori)infection in the remnant stomach after surgery is a matter of controversy.We report a simple and accurate method of 13C-UBT for diagnosis of H.pylori infection in the remnant stomach after gastrectomy.Methods:Eighty patients who had undergone gastrectomy with or without subsequent H.pylori eradication therapy were examined a total of 134 times for H.pylori infection by the 13C-UBT.13C-Urea,10 mg per test,was used in powdered form or in the form of film-coated tablets.Breath samples were collected before and 10,20,and 30 min after ingestion.Mucosal biopsy specimens for bacterial culture and histological examination of the remnant stomach were collected endoscopically after each 13C-UBT test.Results:Factors that confounded the 13C-UBT results in the remnant stomach were oral bacteria,posture,and residual food.Lying horizontally on the left side was the best position,and film-coated tablets indicated no necessity for use of mouthwash.The method of anastomosis had no significant effect on the results of the 13C-UBT.Thirty minutes and a cutoff of 4.5 were optimal conditions for detection of H.pylori in the remnant stomach.Under these conditions,sensitivity,specificity,and accuracy were 79.4%(27/34 cases),95.7%(44/46 cases),and 88.8%(71/80 cases),respectively,in ordinary H.pylori diagnosis,and 100%(2/2 cases),93.3%(14/15 cases),and 94.1%(16/17 cases),respectively,in evaluating eradication at 4 weeks after treatment of H.pylori.Conclusions:Having the patient lie horizontally on the left side,using a film-coated 13C-urea tablet without using mouthwash,and measurement at 30 min provided a simple and accurate method of 13C-urea breath test for detection of H.pylori in the remnant stomach after gastrectomy.展开更多
Background:The 13C-urea breath test(UBT)for detecting Helicobacter pylori infection is a non-invasive method based on the organism’s urease activity.Since its first description,the method has been extensively modifie...Background:The 13C-urea breath test(UBT)for detecting Helicobacter pylori infection is a non-invasive method based on the organism’s urease activity.Since its first description,the method has been extensively modified.However,only the dose of 13C-urea and the measurement equipment are directly related to the cost of the test.Aims:(1)To assess the diagnostic accuracy before eradication therapy of three UBTs using 25,15,and 10 mg of 13C-urea,respectively;and(2)to determine diagnostic performance in the post-eradication setting showing the highest values for sensitivity and specificity with the lowest dose of 13C-urea.Methods:Three hundred consecutive patients were randomised to be tested with one of the three UBTs.All patients underwent upper endoscopy with biopsies.A total of 222 more patients were enrolled to evaluate the second aim.Infected patients were offered treatment and asked to return 4-6 weeks after the end of therapy to perform endoscopic follow up and to carry out 13C-UBT.Results:In the pretreatment setting,13C-UBT 25 mg had a sensitivity of 100%(95%confidence interval(CI)91.8-100)and a specificity of 100%(95%CI 93.7-100);13C-UBT 15 mg had a sensitivity of 96.1%(95%CI 86.8-98.9)and a specificity of 100%(95%CI 92.6-100);and 13C-UBT 10 mg had a sensitivity of 89.1%(95%CI 77-95.3)and a specificity of 100%(95%CI 93.3-100).As the test with the best performance and the lowest dose of 13C-urea was 13C-UBT 15 mg,it was evaluated after treatment.reporting a sensitivity of 100%(95%CI 79.6-100)and a specificity of 98.9%(95%CI 94.3-99.8).Discussion:UBTs using 25 and 15 mg of 13C-urea were both accurate in the diagnosis of H pylori infection in untreated patients.13C-UBT 15mg was also accurate for follow up of patients after treatment.展开更多
文摘^13C-UBT, histology and RUT were used in diagnosing HP infection in this study (gastric and duodenal ulcer, 65 cases; control, 36 cases). Any two tests of the above three are positive, then HP infection is confirmed. Results: The positive rate of ^13C-UBT, histology and RUT are: 81.54%, 83.1%, 84.62%, sensitivity: 96.23%, 86.8%, 84.91%, specificity: 100%, 91.67%, 83.33%. We conclude that ^13C-UBT and histology and accurate in diagnosing HP infection; though the sensitivity and specificity of RUT are lower,
文摘Background:The efficacy of the 13C-urea breath test(UBT)for diagnosis of Helicobacter pylori(H.pylori)infection in the remnant stomach after surgery is a matter of controversy.We report a simple and accurate method of 13C-UBT for diagnosis of H.pylori infection in the remnant stomach after gastrectomy.Methods:Eighty patients who had undergone gastrectomy with or without subsequent H.pylori eradication therapy were examined a total of 134 times for H.pylori infection by the 13C-UBT.13C-Urea,10 mg per test,was used in powdered form or in the form of film-coated tablets.Breath samples were collected before and 10,20,and 30 min after ingestion.Mucosal biopsy specimens for bacterial culture and histological examination of the remnant stomach were collected endoscopically after each 13C-UBT test.Results:Factors that confounded the 13C-UBT results in the remnant stomach were oral bacteria,posture,and residual food.Lying horizontally on the left side was the best position,and film-coated tablets indicated no necessity for use of mouthwash.The method of anastomosis had no significant effect on the results of the 13C-UBT.Thirty minutes and a cutoff of 4.5 were optimal conditions for detection of H.pylori in the remnant stomach.Under these conditions,sensitivity,specificity,and accuracy were 79.4%(27/34 cases),95.7%(44/46 cases),and 88.8%(71/80 cases),respectively,in ordinary H.pylori diagnosis,and 100%(2/2 cases),93.3%(14/15 cases),and 94.1%(16/17 cases),respectively,in evaluating eradication at 4 weeks after treatment of H.pylori.Conclusions:Having the patient lie horizontally on the left side,using a film-coated 13C-urea tablet without using mouthwash,and measurement at 30 min provided a simple and accurate method of 13C-urea breath test for detection of H.pylori in the remnant stomach after gastrectomy.
文摘Background:The 13C-urea breath test(UBT)for detecting Helicobacter pylori infection is a non-invasive method based on the organism’s urease activity.Since its first description,the method has been extensively modified.However,only the dose of 13C-urea and the measurement equipment are directly related to the cost of the test.Aims:(1)To assess the diagnostic accuracy before eradication therapy of three UBTs using 25,15,and 10 mg of 13C-urea,respectively;and(2)to determine diagnostic performance in the post-eradication setting showing the highest values for sensitivity and specificity with the lowest dose of 13C-urea.Methods:Three hundred consecutive patients were randomised to be tested with one of the three UBTs.All patients underwent upper endoscopy with biopsies.A total of 222 more patients were enrolled to evaluate the second aim.Infected patients were offered treatment and asked to return 4-6 weeks after the end of therapy to perform endoscopic follow up and to carry out 13C-UBT.Results:In the pretreatment setting,13C-UBT 25 mg had a sensitivity of 100%(95%confidence interval(CI)91.8-100)and a specificity of 100%(95%CI 93.7-100);13C-UBT 15 mg had a sensitivity of 96.1%(95%CI 86.8-98.9)and a specificity of 100%(95%CI 92.6-100);and 13C-UBT 10 mg had a sensitivity of 89.1%(95%CI 77-95.3)and a specificity of 100%(95%CI 93.3-100).As the test with the best performance and the lowest dose of 13C-urea was 13C-UBT 15 mg,it was evaluated after treatment.reporting a sensitivity of 100%(95%CI 79.6-100)and a specificity of 98.9%(95%CI 94.3-99.8).Discussion:UBTs using 25 and 15 mg of 13C-urea were both accurate in the diagnosis of H pylori infection in untreated patients.13C-UBT 15mg was also accurate for follow up of patients after treatment.
