High-resolution ro-vibrational spectroscopy of ^15N2^16O in 1650-3450 cm-1 region is studied using highly enriched isotopologue sample. The positions of more than 7300 lines of ^15N2^16O isotopologue were measured wit...High-resolution ro-vibrational spectroscopy of ^15N2^16O in 1650-3450 cm-1 region is studied using highly enriched isotopologue sample. The positions of more than 7300 lines of ^15N2^16O isotopologue were measured with a typical accuracy of 5.0×10-4 cm-1. The transitions were rovibrationally assigned on the basis of the global effective Hamiltonian model. The band by band analysis allowed for the determination of the rovibrational parameters of a total of 73 bands. 29 of them are newly reported and more rotational transitions have been observed for the others. The maximum deviation of the preidictions of the effective Hamiltonian model is up to 0.70 cm-1 for the ^15N2^16O species.展开更多
夏季休闲是黄土高原旱地小麦常见的蓄纳雨水、恢复地力的措施。随着氮肥用量的增加,一季小麦收获后,旱地土壤剖面累积的硝态氮量不断增加,休闲期间降雨量高,残留硝态氮的去向是值得研究的问题。利用^(15)N标记法研究小麦收获后残留肥料...夏季休闲是黄土高原旱地小麦常见的蓄纳雨水、恢复地力的措施。随着氮肥用量的增加,一季小麦收获后,旱地土壤剖面累积的硝态氮量不断增加,休闲期间降雨量高,残留硝态氮的去向是值得研究的问题。利用^(15)N标记法研究小麦收获后残留肥料氮在黄土高原旱地(陕西长武)夏季休闲期间的去向,即小麦收获后在微区土壤表层(0~15 cm)施入^(15)N标记的硝态氮肥(30 kg hm^(-2)(以纯氮计),约相当于当地小麦一季作物收获后土壤残留肥料氮量),休闲结束后,采集0~200 cm土壤样品,测定了土壤全氮、硝态氮含量及其^(15)N丰度。结果表明,小麦收获(即休闲开始)时0~200 cm土壤剖面硝态氮累积量在205~268 kg hm-2之间(平均244 kg hm^(-2)),累积量较高。夏季休闲期间降水量为157 mm,属欠水年,但休闲结束后,^(15)N标记肥料氮向下迁移已达80 cm土层,下移深度在45~65cm之间,说明,旱地休闲期间硝态氮的淋溶作用不可忽视。夏季休闲结束后,加入的^(15)N标记肥料氮平均损失率为28%,损失机理值得进一步研究。展开更多
Natural nitrogen isotope composition(δ^(15)N) is an indicator of nitrogen sources and is useful in the investigation of nitrogen cycling in organisms and ecosystems. δ^(15)N is also used to study assimilation of ino...Natural nitrogen isotope composition(δ^(15)N) is an indicator of nitrogen sources and is useful in the investigation of nitrogen cycling in organisms and ecosystems. δ^(15)N is also used to study assimilation of inorganic nitrogen. However, the foliar δ^(15)N of intact plants, which is a consequence of nitrate assimilation occurring in the roots and shoots, is not suited for studying nitrate assimilation in cases where nitrate is the sole nitrogen source. In this study, Orychophragmus violaceus(Ov) and Brassica napus(Bn) plantlets, in which nitrate assimilation occurred in the leaves, were used to study the relationship between foliar δ^(15)N and nitrate assimilation.The plantlets were grown in vitro in culture media with different nitrate concentrations, and no root formation occurred for the plantlets during the multiplication stage.Nitrogen isotope fractionation occurred in both the Ov and the Bn plantlets under all treatments. Furthermore, the foliar nitrogen content of both the Ov and Bn plantlets increased with increasing nitrate concentration. Foliar nitrogen isotope fractionation was negatively correlated with foliar nitrogen content for both the Ov and Bn plantlets. Our results suggest that the foliar nitrogen isotope fractionation value could be employed to evaluate nitrate assimilation ability and leaf nitrate reductase activity.Moreover, high external nitrate concentrations couldcontribute to improved foliar nitrogen content and enhanced nitrate assimilation ability.展开更多
Soil water content significantly influenced uptake and distribution of ^15N in both Newhall and Yamasitaka. The content of ^15N uptake in treated plants was less than that in controlled plants, under 20% soil water co...Soil water content significantly influenced uptake and distribution of ^15N in both Newhall and Yamasitaka. The content of ^15N uptake in treated plants was less than that in controlled plants, under 20% soil water content, ^15N was only taken up 16.02% by Newhall and 10.11% by Yamasitaka. The most ^15N was detained in root and old shoots under water stress. Protein concentration in two cultivars significantly decreased by water deficit stress, protein content of Newhall and Yamasitaka in controlled plants was 16.29 mg/g fresh weight and 15.89 mg/g fresh weight, but at 20% of water content, these were 9.60 mg/g fresh weight and 9.02 mg/g fresh weight. Water stress increased concentration of NH3-NH4^＋, Arginine and Proline. Compared with control plants, concentrations of NH3-NH4^＋ in both Newhall and Yamasitaka at 20% water content treatment increased 5.83 fold and 5.71 fold, Arginine increased 197% and 205%, and Proline increased 112% and 132%.展开更多
Nitrogen is one of the essential nutrient elements for plant growth,which plays an important role in the growth and development of sugarcane. The whole growth cycle of sugarcane needs a large amount of nitrogen. Incre...Nitrogen is one of the essential nutrient elements for plant growth,which plays an important role in the growth and development of sugarcane. The whole growth cycle of sugarcane needs a large amount of nitrogen. Increasing the application of nitrogen can improve the yield of sugarcane,but it will also cause environmental pollution. Therefore,how to control or reduce the application of nitrogen fertilizer while continuously increasing sugarcane yield,reduce the increase of sugarcane production cost and environmental pollution caused by excessive application of nitrogen fertilizer has become an important scientific problem faced by sugarcane industry in China.^15N stable isotope labeling technology has been applied to many crops as a nitrogen research tool. In order to better understand the demand of nitrogen fertilizer in soil-cane system,this paper reviewed nitrogen allocation in plants,nitrogen loss,nitrogen recycling and endogenous nitrogen fixation of sugarcane based on^15N stable isotope labeling technology used in the nitrogen uptake and utilization,providing a theoretical basis for the improvement of sugarcane nitrogen use efficiency and the efficient nitrogen fertilizer management of sugarcane.展开更多
基金This work is supported by the National Natural Science Foundation of China （No.20903085）, the NKBRSF 2010CB9230, and RFBR-Russia （No.06-05- 39016）. The support of the Groupement de Recherche International SAMIA （Spectroscopie d＇Absorption des Mol@cules d＇Interet Atmospherique） between CNRS （France）, RFBR （Russia） and CAS （China） is also acknowledged.
文摘High-resolution ro-vibrational spectroscopy of ^15N2^16O in 1650-3450 cm-1 region is studied using highly enriched isotopologue sample. The positions of more than 7300 lines of ^15N2^16O isotopologue were measured with a typical accuracy of 5.0×10-4 cm-1. The transitions were rovibrationally assigned on the basis of the global effective Hamiltonian model. The band by band analysis allowed for the determination of the rovibrational parameters of a total of 73 bands. 29 of them are newly reported and more rotational transitions have been observed for the others. The maximum deviation of the preidictions of the effective Hamiltonian model is up to 0.70 cm-1 for the ^15N2^16O species.
文摘夏季休闲是黄土高原旱地小麦常见的蓄纳雨水、恢复地力的措施。随着氮肥用量的增加,一季小麦收获后,旱地土壤剖面累积的硝态氮量不断增加,休闲期间降雨量高,残留硝态氮的去向是值得研究的问题。利用^(15)N标记法研究小麦收获后残留肥料氮在黄土高原旱地(陕西长武)夏季休闲期间的去向,即小麦收获后在微区土壤表层(0~15 cm)施入^(15)N标记的硝态氮肥(30 kg hm^(-2)(以纯氮计),约相当于当地小麦一季作物收获后土壤残留肥料氮量),休闲结束后,采集0~200 cm土壤样品,测定了土壤全氮、硝态氮含量及其^(15)N丰度。结果表明,小麦收获(即休闲开始)时0~200 cm土壤剖面硝态氮累积量在205~268 kg hm-2之间(平均244 kg hm^(-2)),累积量较高。夏季休闲期间降水量为157 mm,属欠水年,但休闲结束后,^(15)N标记肥料氮向下迁移已达80 cm土层,下移深度在45~65cm之间,说明,旱地休闲期间硝态氮的淋溶作用不可忽视。夏季休闲结束后,加入的^(15)N标记肥料氮平均损失率为28%,损失机理值得进一步研究。
基金supported by the National Key Research and development Program of China (2016YFC0502602)the National Natural Science Foundation of China (U1612441)the project of high-level innovative talents of Guizhou Province [2015(4035)]
文摘Natural nitrogen isotope composition(δ^(15)N) is an indicator of nitrogen sources and is useful in the investigation of nitrogen cycling in organisms and ecosystems. δ^(15)N is also used to study assimilation of inorganic nitrogen. However, the foliar δ^(15)N of intact plants, which is a consequence of nitrate assimilation occurring in the roots and shoots, is not suited for studying nitrate assimilation in cases where nitrate is the sole nitrogen source. In this study, Orychophragmus violaceus(Ov) and Brassica napus(Bn) plantlets, in which nitrate assimilation occurred in the leaves, were used to study the relationship between foliar δ^(15)N and nitrate assimilation.The plantlets were grown in vitro in culture media with different nitrate concentrations, and no root formation occurred for the plantlets during the multiplication stage.Nitrogen isotope fractionation occurred in both the Ov and the Bn plantlets under all treatments. Furthermore, the foliar nitrogen content of both the Ov and Bn plantlets increased with increasing nitrate concentration. Foliar nitrogen isotope fractionation was negatively correlated with foliar nitrogen content for both the Ov and Bn plantlets. Our results suggest that the foliar nitrogen isotope fractionation value could be employed to evaluate nitrate assimilation ability and leaf nitrate reductase activity.Moreover, high external nitrate concentrations couldcontribute to improved foliar nitrogen content and enhanced nitrate assimilation ability.
基金Acknowledgment The authors acknowledge the financial support of the CARS-3 （China Agricultural Research System） and National Natural Science Foundation of China （31071763） and suggestions of Dr. C.J. Lovatt,Professor of Plant Physiology, University of California, Riverside.
文摘Soil water content significantly influenced uptake and distribution of ^15N in both Newhall and Yamasitaka. The content of ^15N uptake in treated plants was less than that in controlled plants, under 20% soil water content, ^15N was only taken up 16.02% by Newhall and 10.11% by Yamasitaka. The most ^15N was detained in root and old shoots under water stress. Protein concentration in two cultivars significantly decreased by water deficit stress, protein content of Newhall and Yamasitaka in controlled plants was 16.29 mg/g fresh weight and 15.89 mg/g fresh weight, but at 20% of water content, these were 9.60 mg/g fresh weight and 9.02 mg/g fresh weight. Water stress increased concentration of NH3-NH4^＋, Arginine and Proline. Compared with control plants, concentrations of NH3-NH4^＋ in both Newhall and Yamasitaka at 20% water content treatment increased 5.83 fold and 5.71 fold, Arginine increased 197% and 205%, and Proline increased 112% and 132%.
基金Supported by National Natural Science Foundation of China(31860350)Guangxi Project(GK AA17202042-6)+2 种基金Earmarked Fund for China Agriculture Research System(CARS-170105)Guangxi Innovation Team Program(gjnytxgxcxtd-03-01)Fund of Guangxi Academy of Agricultural Sciences(GNK2018YT02,2018YM01,2020YM24)。
文摘Nitrogen is one of the essential nutrient elements for plant growth,which plays an important role in the growth and development of sugarcane. The whole growth cycle of sugarcane needs a large amount of nitrogen. Increasing the application of nitrogen can improve the yield of sugarcane,but it will also cause environmental pollution. Therefore,how to control or reduce the application of nitrogen fertilizer while continuously increasing sugarcane yield,reduce the increase of sugarcane production cost and environmental pollution caused by excessive application of nitrogen fertilizer has become an important scientific problem faced by sugarcane industry in China.^15N stable isotope labeling technology has been applied to many crops as a nitrogen research tool. In order to better understand the demand of nitrogen fertilizer in soil-cane system,this paper reviewed nitrogen allocation in plants,nitrogen loss,nitrogen recycling and endogenous nitrogen fixation of sugarcane based on^15N stable isotope labeling technology used in the nitrogen uptake and utilization,providing a theoretical basis for the improvement of sugarcane nitrogen use efficiency and the efficient nitrogen fertilizer management of sugarcane.
