The Hu-IFN-αgene,which was transducted into downstream promoter of β-actin gene of common carp（Cyprinus carpio）,was recombined by DNA recombination technology.These recombined genes were injected into 1-2 cell fer...The Hu-IFN-αgene,which was transducted into downstream promoter of β-actin gene of common carp（Cyprinus carpio）,was recombined by DNA recombination technology.These recombined genes were injected into 1-2 cell fertilized eggs of grass carp（Ctenophatyngodon idellus） by micro-injection technology,we gained transgenetic fish by molecular detection methods.In order to analyse the genetic expression of tran-Hu-IFN-α gene gynogensis F1,which male individualization were gained by raising methyl-testosterone,molecular genetic marker technology was used.In our research,30 random primers were picked out from 48 and were used into RAPD-PCR,the result indicated that 1 169 clear,steady and repeated DNA finger printing bands were achieved.On the basis of gentic distance matrix among tran-Hu-IFN-α gene gynogenesis F1 group,the genetic relationship of gynogenesis F1 were analysed by UPGMA,the results showed the genetic patterns are close between the 3# male gynogenesis F1 and the the 23# female of gynogenesis F1,5# and 27#,2# and 28#,2# and 30#.The data indicated that these group could be served as parent of tran-Hu-IFN-α grass carp（Ctenophatyngodon idellus） pure line.展开更多
文摘The Hu-IFN-αgene,which was transducted into downstream promoter of β-actin gene of common carp（Cyprinus carpio）,was recombined by DNA recombination technology.These recombined genes were injected into 1-2 cell fertilized eggs of grass carp（Ctenophatyngodon idellus） by micro-injection technology,we gained transgenetic fish by molecular detection methods.In order to analyse the genetic expression of tran-Hu-IFN-α gene gynogensis F1,which male individualization were gained by raising methyl-testosterone,molecular genetic marker technology was used.In our research,30 random primers were picked out from 48 and were used into RAPD-PCR,the result indicated that 1 169 clear,steady and repeated DNA finger printing bands were achieved.On the basis of gentic distance matrix among tran-Hu-IFN-α gene gynogenesis F1 group,the genetic relationship of gynogenesis F1 were analysed by UPGMA,the results showed the genetic patterns are close between the 3# male gynogenesis F1 and the the 23# female of gynogenesis F1,5# and 27#,2# and 28#,2# and 30#.The data indicated that these group could be served as parent of tran-Hu-IFN-α grass carp（Ctenophatyngodon idellus） pure line.
