迷迭香叶中三萜酸类成分及其抑菌活性分析

Triterpene acids components in leaves of Rosmarinus officinalis Linn.were separated and identified by HPLC,silica gel column chromatography and reversed-phase C18 semi-preparative liquid chromatography method.And taking the minimum inhibitory concentration(MIC)as the index,the inhibitory activity of each compound was determined.The results show that 4 triterpene acids compounds are isolated from ethanol extracts of leaves of R.officinalis;according to the peak characteristics of reference substances,compound 2,3 and 4 are identified as betulinic acid,oleanolic acid and ursolic acid,respectively;based on physicochemical constants and spectral data,compound 1 is identified asΔ^20(30)-ene-ursolic acid.Ursolic acid,Δ^20(30)-ene-ursolic acid and oleanolic acid have a certain inhibitory activity to Gram-positive strains(methicillin-resistant Staphylococcus aureus,MRSA),in which,the MIC value of ursolic acid to MRSA is 8.000-128.000μg·mL-1,and its inhibitory activity is the strongest;however,none of 4 compounds shows inhibitory activity to Gram-negative strain(Escherichia coli S002).

小驳骨中萜类化学成分研究

目的研究小驳骨Gendarussa vulgaris地上部位的萜类化学成分。方法采用硅胶、Sephadex LH-20、反相ODS、大孔吸附树脂AB-8等柱色谱方法以及半制备HPLC法进行分离纯化,根据化合物理化性质和波谱数据进行结构鉴定。结果从小驳骨药材95%乙醇总提取物中共分离得到10个萜类化合物,分别鉴定为驳骨萜A(1)、4,4,14α-三甲基孕甾-8-烯-3β,20α-二醇(2)、华木酮(3)、过氧化麦角甾醇(4)、熊果酸(5)、齐墩果酸(6)、3β-羟基-11α,12α-环氧齐墩果-28,13β-内酯(7)、当药苷(8)、马钱子苷(9)、脱水莫诺苷元(10)。结论化合物1为未见文献报道的新化合物,命名为驳骨萜A;化合物2为新天然产物,并首次对其氢谱和碳谱数据进行归属;化合物3~5、7~10均为首次从该属植物中分离得到。