The influence of different NH 4 +/NO 3 - ratios on nitrogen metabolism of cotton was studied under controlled hydroponics.The results showed that compared with single nitrate nutrition,solutions with 25/75,50/50,75/25...The influence of different NH 4 +/NO 3 - ratios on nitrogen metabolism of cotton was studied under controlled hydroponics.The results showed that compared with single nitrate nutrition,solutions with 25/75,50/50,75/25 and 100/0 of NH 4 +/NO 3 - significantly increased the soluble protein accumulation in leaves and roots of cotton,and the maximum content of soluble protein in leaves and roots appeared respectively in the solution with 50/50 and 75/25 of NH 4 +/NO 3 -.The soluble protein content in roots was increased with the increase of NH + 4 percentage,but was slightly less in the solution of 100/0 than 75/25,which was probably related to the excess NH + 4 limiting boot metabolism.With the increase of NH + 4 percentage,the nitrate content in petiole and the nitrate reductase activity in functional blade declined,but ammoniac nitrogen content increased in every organ of cotton.These results showed that foreign nitrogen affected the nitrogen metabolism of cotton in a different way,and the nitrogen absorption by cotton was probably related to different forms of foreign nitrogen.展开更多
目的:以扁桃体隐窝内甲型链球菌(HS)灭活菌株刺激IgA肾病(IgAN)患者和非肾炎患者扁桃体淋巴细胞,观察未刺激及刺激后CD4+CD25+细胞和分泌J链IgA细胞数量,探讨IgAN的发病机制。方法:(1)收集37例IgAN患者及37例非肾炎慢性扁桃体炎患者手...目的:以扁桃体隐窝内甲型链球菌(HS)灭活菌株刺激IgA肾病(IgAN)患者和非肾炎患者扁桃体淋巴细胞,观察未刺激及刺激后CD4+CD25+细胞和分泌J链IgA细胞数量,探讨IgAN的发病机制。方法:(1)收集37例IgAN患者及37例非肾炎慢性扁桃体炎患者手术摘除的扁桃体;(2)分离鉴定两组患者扁桃体隐窝内细菌及分离培养扁桃体淋巴细胞;(3)以分离最多的灭活菌株HS体外刺激扁桃体淋巴细胞72h;(4)以流式细胞仪检测扁桃体淋巴细胞CD4+CD25+细胞数,以原位杂交技术检测J链mRNA表达,以免疫荧光及荧光原位杂交技术同步分析分泌J链IgA细胞。结果:(1)两组患者均有甲型链球菌,且甲型链球菌在分离的细菌中是最多的。两组患者的细菌谱和细菌量无统计学差异。(2)未刺激、非肾炎患者HS(HS-controls)、IgAN患者HS(HS-IgAN)刺激后CD4+CD25+细胞数[(0.98±0.204)% vs (3.58±0.554)%,P<0.05,(1.37±0.214)% vs (5.78±0.562)%,P<0.05,and(1.43±0.202)% vs (6.05±0.521)%,P<0.05],IgAN组与非肾炎组比较,前者均显著低于后者。HS对IgAN组CD4+CD25+细胞的刺激指数(stimulation index,SI)显著低于非肾炎组(P均<0.05)。(3)未刺激、HS-controls、HS-IgAN刺激后J链mRNA阳性IgA细胞[(11.9±3.1)% vs (6.5±1.5)%,P<0.05,(33.5±5.7)% vs (13.9±1.2)%,P<0.05,and(35.1±6.2)% vs (13.9±1.2)%,P<0.01],IgAN组与非肾炎组比较,前者均显著高于后者。HS对IgAN组J链mRNA阳性IgA细胞的SI显著高于非肾炎组(P均<0.01)。(4)HS对CD4+CD25+细胞的SI与对分泌J链IgA细胞的SI呈显著性负相关(P均<0.01)。结论:IgAN患者扁桃体CD4+CD25+细胞减少和分泌J链IgA细胞增多可能与IgAN的发病机制有关。展开更多
文摘The influence of different NH 4 +/NO 3 - ratios on nitrogen metabolism of cotton was studied under controlled hydroponics.The results showed that compared with single nitrate nutrition,solutions with 25/75,50/50,75/25 and 100/0 of NH 4 +/NO 3 - significantly increased the soluble protein accumulation in leaves and roots of cotton,and the maximum content of soluble protein in leaves and roots appeared respectively in the solution with 50/50 and 75/25 of NH 4 +/NO 3 -.The soluble protein content in roots was increased with the increase of NH + 4 percentage,but was slightly less in the solution of 100/0 than 75/25,which was probably related to the excess NH + 4 limiting boot metabolism.With the increase of NH + 4 percentage,the nitrate content in petiole and the nitrate reductase activity in functional blade declined,but ammoniac nitrogen content increased in every organ of cotton.These results showed that foreign nitrogen affected the nitrogen metabolism of cotton in a different way,and the nitrogen absorption by cotton was probably related to different forms of foreign nitrogen.
文摘目的:以扁桃体隐窝内甲型链球菌(HS)灭活菌株刺激IgA肾病(IgAN)患者和非肾炎患者扁桃体淋巴细胞,观察未刺激及刺激后CD4+CD25+细胞和分泌J链IgA细胞数量,探讨IgAN的发病机制。方法:(1)收集37例IgAN患者及37例非肾炎慢性扁桃体炎患者手术摘除的扁桃体;(2)分离鉴定两组患者扁桃体隐窝内细菌及分离培养扁桃体淋巴细胞;(3)以分离最多的灭活菌株HS体外刺激扁桃体淋巴细胞72h;(4)以流式细胞仪检测扁桃体淋巴细胞CD4+CD25+细胞数,以原位杂交技术检测J链mRNA表达,以免疫荧光及荧光原位杂交技术同步分析分泌J链IgA细胞。结果:(1)两组患者均有甲型链球菌,且甲型链球菌在分离的细菌中是最多的。两组患者的细菌谱和细菌量无统计学差异。(2)未刺激、非肾炎患者HS(HS-controls)、IgAN患者HS(HS-IgAN)刺激后CD4+CD25+细胞数[(0.98±0.204)% vs (3.58±0.554)%,P<0.05,(1.37±0.214)% vs (5.78±0.562)%,P<0.05,and(1.43±0.202)% vs (6.05±0.521)%,P<0.05],IgAN组与非肾炎组比较,前者均显著低于后者。HS对IgAN组CD4+CD25+细胞的刺激指数(stimulation index,SI)显著低于非肾炎组(P均<0.05)。(3)未刺激、HS-controls、HS-IgAN刺激后J链mRNA阳性IgA细胞[(11.9±3.1)% vs (6.5±1.5)%,P<0.05,(33.5±5.7)% vs (13.9±1.2)%,P<0.05,and(35.1±6.2)% vs (13.9±1.2)%,P<0.01],IgAN组与非肾炎组比较,前者均显著高于后者。HS对IgAN组J链mRNA阳性IgA细胞的SI显著高于非肾炎组(P均<0.01)。(4)HS对CD4+CD25+细胞的SI与对分泌J链IgA细胞的SI呈显著性负相关(P均<0.01)。结论:IgAN患者扁桃体CD4+CD25+细胞减少和分泌J链IgA细胞增多可能与IgAN的发病机制有关。
