高分辩气相色谱法手性固定相拆分α-羟基酮光学异构体

使用高分辩气相色谱法环糊精接枝聚硅氧烷 (二环〔2 ,6 二 O 戊基 3 O 己烯基 ( 5 )〕五环〔2 ,6 二 O 戊基 3 O 甲基〕 β 环糊精 聚硅氧烷 )作手性固定相 ,实现了α 羟基酮的有效拆分 ,并测定了一系列光学活性 3 羟基 2 丁酮的对映体过剩值 (e .e值 )。结果表明 ,所选取的手性固定相对测定此类不对称催化反应产物的光学产率 ,评价催化体系的手性选择性均具有较好的效果。

培养条件对赭曲霉菌球形成和坎利酮11α羟基化的影响

菌丝粗壮、分布均匀的菌球可提高赭曲霉11α羟基化的效率。控制赭曲霉菌球直径的平均大小可以通过选择不同的培养温度、装液量、以及转速等因素而达到。通过正交分析，确定培养温度为30℃，按孢子悬液浓度为10。个／mL接种，装液量为50mL／250mL三角瓶，摇床转速为200r／min时，菌球达到最佳直径2～3rnrn之间的百分比最大。在此条件下，以10g/L的坎利酮投料，最终转化率高达91．26％。

α羟基环十五酮及3-羟基,甲基环十五酮合成麝香酮的方法

麝香酮是一种名贵的香料,用途广泛但来源稀缺。近半个世纪以来麝香酮的化学合成方法日渐丰富,但实现工业化的很少。从α羟基环十五酮和3-羟基,甲基环十五酮的属性研究中,总结出插入甲基法合成麝香酮的优越性。

深层培养中赭曲霉菌球对坎利酮11α羟化反应影响的研究

菌球的松散程度和菌球直径通过调节发酵培养基的初始pH和接种量来实现。实验结果证明直径越小,结构越松散的菌球可以获得高11α羟基坎利酮转化率。7L罐可以形成颗粒小,且松散的菌球。终了转化率92%,且节省了发酵时间。

深层培养中赭曲霉菌球催化坎利酮11α羟化反应条件的研究

研究了氮源、碳源对11α羟基坎利酮转化率的影响。从赭曲霉菌球形态的角度解释了菌球直径、结构与11α羟基坎利酮转化率之间的关系。发现外部菌丝粗壮,分布均匀,内部菌丝虽有分化,但分化不明显的菌球能获得高11α羟基坎利酮转化率。

Technologies of Seperating and Exactly 11-α-Hydroxy-Canerenone from Biotransformation Broth

[Objective] The aim was to investigate the effect of four extraction meth- ods on extraction ratio of 11-α-Hydroxy-Canerenone from biotransformation broth. [Method] Previous reported method, soaking, elution method and homogenate meth- ods were used. [Result] Results showed that more than 90% of the biotransformed 11-α-Hydroxy-Canerenone existed in the broth supernatant and mycelia surface. Elu- tion and soaking methods were effective for 11-α-Hydroxy-Canerenone extraction. Extraction yields of 96.0% and 98.8% were obtained by eluting with 400 ml of ethy- lacetate and soaking with ethylacetate for 90 min, respectively. [Conelusion] The method is simple and efficient, showing industrial application potential.

Br?nsted acidic ionic liquid catalyzed synthesis of benzo[a]carbazole from renewable acetol and 2-phenylindoles in a biphasic system

An efficient metal-free strategy for the synthesis of pharmaceutically relevant benzo[α]carbazoles from the derivatives of readily available 2-phenylindole and bio-renewable acetol in an aqueous biphasic system was developed. This protocol employed a sulfone-containing Bronsted acidic ionic liquid as the catalyst, which could be used for five times without a noticeable decrease in its activity and selectivity. Various substituted 2-phenylindoles and α-hydroxyketones participated in the reaction smoothly, with water as the sole byproduct. Mechanistically, the reaction involved the conventional carbon-nucleophile-induced Heyns-type rearrangement and downstream intramolecular olefination.

Synthesis of α-hydroxy ketones by copper（Ⅰ）-catalyzed hydration of propargylic alcohols: CO2 as a cocatalyst under atmospheric pressure

Inexpensive and efficient Cu(Ⅰ) catalysis is reported for the synthesis of α-hydroxy ketones from propargylic alcohols, CO2, and water via tandem carboxylative cyclization and nucleophilic addition reaction. Notably, hydration of propargylic alcohols can be carried out smoothly under atmospheric CO2 pressure, generating a series of α-hydroxy ketones efficiently and selectively. This strategy shows great potential for the preparation of valuable α-hydroxy ketones by using CO2 as a crucial cocatalyst under mild conditions.

赭曲霉的高密度培养对坎利酮转化的影响

目的:研究赭曲霉高密度培养的发酵培养基及条件,实现坎利酮的高转化。方法:选取廉价易得的培养基成分并进行优化,同时对发酵条件进行优化,得到了最优发酵培养基配方及培养条件。结果:发酵培养基最优配方为:葡萄糖20g/L,玉米浆20g/L,酵母膏20g/L,K2HPO4 2.5g/L。种子液最佳培养时间为24 h,发酵培养基初始pH 5.8,接种量为8%,装液量200mL/1 000mL,摇床转速为180 r/min,28℃,底物投料时间24 h,发酵结束时间72 h。结论:将该工艺在7 L发酵罐中放大,菌体密度达到25.36g/L,11α羟基坎利酮的转化率为86.1%。