本文主要利用三价膦与邻二羰基化合物的Kukhtin-Ramirez反应中间体原位构筑五配体磷类化合物作为配体偶联的反应平台,一步法实现了α-氨基酸酯类化合物的高效合成。该方法创新性地利用了Kukhtin-Ramirez中间体原位转化为五配体磷类化合...本文主要利用三价膦与邻二羰基化合物的Kukhtin-Ramirez反应中间体原位构筑五配体磷类化合物作为配体偶联的反应平台,一步法实现了α-氨基酸酯类化合物的高效合成。该方法创新性地利用了Kukhtin-Ramirez中间体原位转化为五配体磷类化合物,为需要预合成五配体磷类化合物的传统配体偶联反应模式提供了新的探索方向。In this work, the Kukhtin-Ramirez reaction intermediates from trivalent phosphine and α-dicarbonyl compounds were used to in situ construct pentacoordinated phosphorus compounds as the reaction platform of ligand coupling for the synthesis of α-amino carboxylic esters which was realized in one-step. This approach innovates in the in-situ conversion of Kukhtin-Ramirez inter-mediates into pentacoordinated phosphorus compounds, providing a new direction for the traditional ligand coupling reaction mode that requires pre-synthesis of pentacoordinated phosphorus compounds.展开更多
目的:建立一种简单、快速、可同时检测22种氨基酸的色谱方法,并利用此方法对树莓中的游离氨基酸进行检测和分析,为树莓的开发提供科学依据。方法:以2,4-二硝基氟苯为柱前衍生试剂,Hypersil ODS2色谱柱,流动相A为40 mmol/L p H 6.4乙酸...目的:建立一种简单、快速、可同时检测22种氨基酸的色谱方法,并利用此方法对树莓中的游离氨基酸进行检测和分析,为树莓的开发提供科学依据。方法:以2,4-二硝基氟苯为柱前衍生试剂,Hypersil ODS2色谱柱,流动相A为40 mmol/L p H 6.4乙酸钠溶液,流动相B为体积分数50%乙腈-水溶液,梯度洗脱,反相高效液相色谱,二极管阵列检测器,检测波长360 nm。结果:22种氨基酸线性方程的相关系数范围为0.993 1~1.000 0,相对标准偏差在0.95%~4.98%之间,加标回收率为97.10%~103.39%,最低检出限为0.39~2.87μg/m L,定量限为1.29~9.47μg/m L。利用所建方法检测出树莓中含有14种游离氨基酸,其中6种人体必需氨基酸,占总氨基酸含量的29.36%。结论:所建方法操作简便、重复性好,树莓氨基酸含量丰富,有开发价值。展开更多
文摘本文主要利用三价膦与邻二羰基化合物的Kukhtin-Ramirez反应中间体原位构筑五配体磷类化合物作为配体偶联的反应平台,一步法实现了α-氨基酸酯类化合物的高效合成。该方法创新性地利用了Kukhtin-Ramirez中间体原位转化为五配体磷类化合物,为需要预合成五配体磷类化合物的传统配体偶联反应模式提供了新的探索方向。In this work, the Kukhtin-Ramirez reaction intermediates from trivalent phosphine and α-dicarbonyl compounds were used to in situ construct pentacoordinated phosphorus compounds as the reaction platform of ligand coupling for the synthesis of α-amino carboxylic esters which was realized in one-step. This approach innovates in the in-situ conversion of Kukhtin-Ramirez inter-mediates into pentacoordinated phosphorus compounds, providing a new direction for the traditional ligand coupling reaction mode that requires pre-synthesis of pentacoordinated phosphorus compounds.
