Regulation of <i>α</i>-Melanocyte-Stimulating Hormone by Testosterone Is Associated with the Onset of Atopic Dermatitis Symptoms during Exercise

Objective: Atopic dermatitis (AD) symptoms are altered with exercise. However, no study has investigated the association between testosterone and AD. The aim of this study was to investigate the role of testosterone by which the strength and weakness of exercise affects the skin symptoms of AD. Methods: Specific pathogen-free (SPF) and conventional NC/Nga mice were used. NC/Nga mice spontaneously developed dermal symptoms similar to AD patients. Two exercises, mild (20 m/min, 60 min) and rigorous (25 m/min, 90 min), were carried out using a treadmill four times every alternate day. Furthermore, we administered testosterone (0, 5, 50, 500, and 5000 pg/mice) to non-exercised conventional NC/Nga mice. On the final day of this experiment, we analyzed the plasma levels of immunoglobulin E (IgE), interleukin (IL)-6, IL-4, IL-13, testosterone and α-melanocyte-stimulating hormone (α-MSH) by ELISA kit. Results: Symptoms manifested by NC/Nga mice were strongly exacerbated upon severe exercise but were ameliorated during mild exercise. Between mild and severe exercised conventional mice, the plasma level of IgE was not changed. On administering an equivalent amount of testosterone, depending on the exercise, AD-like symptoms in non-exercising NC/Nga mice were ameliorated with mild exercise and exacerbated through rigorous exercise. Plasma IL-6, IL-4, and IL-13 levels remained unchanged between +50 pg (mild) and +500 pg (severe) testosterone administration. Plasma α-MSH levels were elevated with +500 pg testosterone but decreased with +50 pg testosterone administration. Conclusion: The present results suggest that exercise largely mimics AD symptoms depending on the α-MSH and testosterone levels.

促甲状腺激素(TSH)检测方法小析

促甲状腺激素(Thyroid stimulating hormone,thyrotropin,TSH)由脑垂体前叶的嗜碱性细胞分泌,以游离的形式存在于血液中,含量很低,性质稳定的一种糖蛋白激素。主要生理功能是促进甲状腺的发育和分泌,从而影响全身的代谢。鉴于TSH检测结果的重要临床意义,其检测方法有很多,主要有放射免疫分析,免疫放射分析等。随着生物、化学以及材料等学科的发展,科学家们正在努力寻求更准确、更便捷、低成本的检测方法,为内分泌疾病的临床诊断提供快速、准确的数据。

PRL细胞腺瘤过度分泌对促性腺激素水平及性腺功能的影响

利用RIA测定PRL细胞腺瘤患者血清中PRL、FSH、LH、T水平,探讨PRL细胞腺瘤过度分泌PRL对促性腺激素(GTH)以及对男性性功能的影响。结果在366例PRL细胞腺瘤患者中,159例(43.4％)血清FSH水平低于正常,148例(40.4％)血清LH水平低于正常,8例(2.18％)血清FSH水平高于正常,10例(2.7％)血清LH水平高于正常。在98例男性性功能低下的PRL细胞腺瘤患者中,血清FSH、LH水平与正常对照及性功能正常的PRL细胞腺瘤患者之间无显著性差异,而血清T水平有明显差异(P<0.01和P<0.05)。另外比较血清T/LH比值发现性功能低下组显著低于正常对照组。结果提示,PRL过渡分泌干扰下丘脑-垂体-性腺轴的功能活动,影响性腺对性激素的合成或转化,导致性功能低下。

桂枝茯苓胶囊联合米非司酮治疗子宫肌瘤(?瘀血阻络?)随机平行对照研究

[目的]观察桂枝茯苓胶囊联合米非司酮治疗子宫肌瘤(瘀血阻络)疗效。[方法]使用随机平行对照方法,将100例门诊患者按随机数字表方法随机分为两组。对照组50例米非司酮,12.5mg/次,1次/d,口服。治疗组50例桂枝茯苓胶囊,月经来潮第3d开始,0.93g/次,3次/d;米非司酮治疗同对照组。连续治疗4个月为1疗程。观测临床症状、月经量、经期、下腹痛、子宫体积、瘤体体积、雌二醇、黄体生成素、卵泡刺激素、不良反应。治疗1疗程(4个月),判定疗效。[结果]治疗组痊愈27例,显效13例,有效10例,无效0例,总有效率100.00%;对照组痊愈12例,显效14例,有效13例,无效11例,总有效率78.00%;治疗组疗效优于对照组(P<0.01)。子宫体积、肌瘤体积、E2、FSH、LH两组均有改善(P<0.01,P<0.05),治疗组改善优于对照组(P<0.01,P<0.05)。不良反应发生率治疗组低于对照组(P<0.05)。[结论]桂枝茯苓胶囊联合米非司酮治疗子宫肌瘤(瘀血阻络),疗效满意,无严重不良反应,值得推广。

桥本甲状腺炎合并甲状腺癌的临床特征分析

目的分析桥本甲状腺炎合并甲状腺癌的临床特征，为正确诊断及治疗提供依据。方法回顾性分析87例桥本甲状腺炎合并甲状腺癌患者和105例桥本甲状腺炎患者的临床资料。结果在年龄〈30、30～39、40～49、50～59和≥60岁患者中，桥本甲状腺炎合并甲状腺癌所占的比例分别为1／3、47．5％（29／61）、51．4％（38／74）、36．0％（18／50）和1／4。在30～39岁和40～49岁患者中，桥本甲状腺炎合并甲状腺癌所占的比例高于≥60岁患者，但差异无统计学意义（x2=0．327、0．418，P〉0．05）。桥本甲状腺炎合并甲状腺癌患者和桥本甲状腺炎患者总甲状腺素（TT4）、促甲状腺激素（TSH）、甲状腺球蛋白抗体（TgAb）、甲状腺过氧化物酶抗体（TPOAb）比较差异有统计学意义（P〈0．05或〈0．01）。多因素Logistic回归分析结果表明，血清TT4减低和TSH、TgAb、TPOAb水平增高与桥本甲状腺炎合并甲状腺癌的发生独立相关（P〈0．05或〈0．01）。结论血清TT4减低，TSH、TgAb、TPOAb增高可增加桥本甲状腺炎合并甲状腺癌的风险。临床工作中，对于30～49岁的男性桥本甲状腺炎患者，应高度怀疑其合并甲状腺癌的可能性。

亚临床甲状腺功能减退患者促甲状腺素与颈部动脉内膜中层厚度和抵抗素相关性分析

目的近年来,亚临床甲状腺功能减退(subclinicalhypothyroidism,SCH)检出率明显增加,而SCH可增加心、脑血管疾病的发生风险。本研究旨在探讨健康查体中SCH患者促甲状腺素(thyroid-stimulatinghormone,TSH)与颈动脉内膜中层厚度(intima-median thickness,IMT)和抵抗素(resistin,RE)的关系。方法选择2016-05-09-2017-08-30泰安市第四人民医院健康查体的铁路职工中初诊未经治疗的SCH患者68例作为研究对象,其中4.7mU/L <TSH<10mU/L)36例为A组,TSH≥10mU/L 32例为B组,另选健康者40名为对照组。分别测定三组体质量指数(body mass index,BMI)、TSH、游离三碘甲状腺原氨酸(free T3,FT3)、游离甲状腺激素(free T4,FT4)、空腹血糖(fasting blood glucose,FBG)、三酰甘油(triglycerid,TG)、总胆固醇(cholesterol,TC)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、IMT和RE。结果 TSH水平A组为(7.46±1.49)mU/L,B组为(12.11±1.03)mU/L,均高于对照组的(2.53±0.81)mU/L,F=41.26,P<0.001。TG水平A组为(2.31±1.02)mmol/L,B组为(2.92±0.91)mmol/L,均高于对照组的(1.60±0.41)mmol/L,F=3.87,P=0.025。TC水平A组为(5.02±0.49)mmol/L,B组为(5.56±0.63)mmol/L,均高于对照组的(4.51±0.52)mmol/L,F=3.90,P=0.023。LDL-C水平A组为(3.16±0.43)mmol/L,B组为(3.81±0.49)mmol/L,均高于对照组的(2.45±0.55)mmol/L,F=3.95,P=0.021。A组IMT为(1.02±0.16)mm,B组为(1.23±0.15)mm,均高于对照组的(0.75±0.09)mm,F=46.71,P<0.001。A组RE为(59.13±6.42)μg/L,B组为(52.69±5.33)μg/L,均低于对照组的(66.05±7.35)μg/L,F=52.61,P<0.001。TSH分别与TC(r=0.293,P<0.05)、TG(r=0.326,P<0.05)、LDL-C(r=0.352,P<0.05)和IMT(r=0.337,P<0.05)呈正相关,而TSH与RE呈负相关(r=-0.766 5,P<0.05)。利用多元逐步回归分析,进入IMT为因变量的回归方程的为TSH(β=0.265,t=2.161,P=0.036),TC(β=0.316,t=5.459,P=0.019);进入RE为因变量的回归方程的为TSH(β=-0.322,t=-2.568,P=0.012)、TC(β=-0.296,t=-2.382,P=0.027)和LDL-C(β=-0.410,t=-3.217,P=0.004)。结论 TSH分别与TC、TG、LDL-C、IMT呈正相关,与RE呈负相关。TSH水平可影响颈动脉IMT、RE和血脂代谢,从而使SCH可通过影响患者脂代谢、免疫炎症反应及血管动脉硬化,增加患者心、脑血管疾病的发生风险。

Changes of Serum Follicular Stimulating Hormone，Luteinizing Hormone and Follicular Sizes during Electroacupuncture for Induction of

Changes in follicular stimulating hormone (FSH) . luteinizing hormone (LH) and follicularsizes were observed in 10 patients with chronic anovulation during electroacupuncture treatment. Sevencases were diagnosed as suffering from polycystic ovary syndrome, 2 from dysfunctional uterine bleeding,and 1 from hypogonadotropic amenorrhea. Among them 8 cases complained of infertility for an average of2. 7years. Ovulation was confirmed by pregnancy or the combination of biphasic basal body temperatureand ultrasonographic signs. During one course of 3 consecutive days of electroacupuncture treatment onacupoints Guanyuan (Ren 4), Zhongji (Ren 3), Zigong (Extra 16) and Sanyingjiao (SP 6). ovulation re-sulted in 5 patients (ovulating group) and 3 of the 4 infertile women became pregnant. Five cases failed toovulate (non-ovulating group) , 3 of them reached a biphasic basal body temperature without ovulatorysigns on ultrasonograph. Serum FSH, LH values and FSH pulse frequency increased significantly during-electroacupuncture treatment in the ovulating group (from 2. 10±0. 42 pulses/4h to 3. 70±1. 64 pulses/4h), but not in the non-ovulating group. No apparent change was found in LH pulse frequency, or in pulseamplitudes for FSH and LH. In the ovulating group, diameters of ovarian follicules markedly increased,but their growth was limited in the non-ovulation group. The results suggest ovulation may be induced byelectroacupuncture via a regulation on the hypothalamic-pituitary function, leading to normal secretion ofFSH and LH.

水解贝壳硬蛋白美白功效的初步研究

研究水解贝壳硬蛋白(Shell protein extract,SPE)对B16细胞内黑色素含量及酪氨酸酶活性的影响。培养B16细胞,构建α-黑素细胞刺激素(α-MSH)诱导细胞的黑色素高表达量细胞模型。SPE作用B16细胞,CCK8法测定细胞活力;Na OH裂解法和L-DOPA氧化法测定细胞内黑色素含量和酪氨酸酶活性的变化。结果显示,SPE在1,0.1 g/L时,对细胞无毒性作用;与阳性对照熊果苷相比,SPE对黑色素含量和酪氨酸酶活性的抑制作用与熊果苷基本一致,表明SPE的美白效果与普遍认可的美白添加剂熊果苷的效果一样。