目的分析ERβ2、ERα46在甲状腺乳头状癌(PTC)的表达状态、临床价值及意义。方法选择手术完整切除的甲状腺乳头状癌40份、正常甲状腺组织40份、结节性甲状腺肿40份。采用免疫组织化学技术(Immunohistochemistry,I H C)检测组织中E Rβ2...目的分析ERβ2、ERα46在甲状腺乳头状癌(PTC)的表达状态、临床价值及意义。方法选择手术完整切除的甲状腺乳头状癌40份、正常甲状腺组织40份、结节性甲状腺肿40份。采用免疫组织化学技术(Immunohistochemistry,I H C)检测组织中E Rβ2、 E Rα46表达情况,收集P T C患者术前淋巴结转移情况、临床分期及5年生存率。结果 E Rβ2、ERα46在甲状腺乳头状癌阳性表达率分别为92.5%、70.0%,2种受体在甲状腺乳头状癌和良性病变中的阳性表达差异均有统计学意义(P <0.05)。ERβ2、ERα46诊断乳头状癌的灵敏度分别为92.5%、70.0%,特异度分别为72.5%、78.8%;E Rβ2、 E Rα46的表达与甲状腺乳头状癌的临床分期及淋巴结转移有关,差异均有统计学意义(P <0. 05), 5年生存率、死亡患者ERβ2、ERα46阳性细胞率的差异无统计学意义(P>0.05)。结论 ERβ2、ERα46在甲状腺乳头状癌中表达增强,在病理诊断中有一定的价值,但对于判断远期预后的情况不明显。展开更多
Background Estrogen is involved in suppression of colon cancer development and exerts its function via estrogen receptors α and β （ERa, ERβ）. The recently identified ERα46 resulted from exon 1-deletion from the ...Background Estrogen is involved in suppression of colon cancer development and exerts its function via estrogen receptors α and β （ERa, ERβ）. The recently identified ERα46 resulted from exon 1-deletion from the 66-kDa full length form of ERa66 is devoid of the transactivation domain AF-1, whose function remains largely unknown. Methods In this study, we compared the expression of ERa46 mRNA in 32 normal colorectal tissues and their matched colorectal cancer tissues by real-time quantitative polymerase chain reaction （PCR）. Human colon adenocarcinoma cell HT-29, that has low endogenous expression of ERα46, was transfected with ERα46-expression vector; methyl thiazolyl tetrazolium （MTT） assay, flow cytometry, DNA fragmentation and TUNEL staining were used to evaluate the proliferation and apoptosis status of the cells in the presence of 17β-oestradiol. Results Higher ERα46 mRNA levels were observed in normal colorectal tissues than in the corresponding cancer tissues. ERα46-transfected cells showed a significantly decreased growth rate than control cells and an accumulation of cells in the G0/1 phase and a reduced proportion of cells in G2/M phase after exposed to 10^-8 mol/L 17β-oestradiol. There were also more positive TUNEL stained cells in ERα46-transfected cells than the control cells in the presence of 17β-oestradiol （P 〈0.05）. Conclusions These data suggest that ERα46 may be involved in the development and/or progression of colorectal cancer via mediating growth inhibition and apoptosis of cancer cells in the presence of 17β-oestradiol.展开更多
文摘目的分析ERβ2、ERα46在甲状腺乳头状癌(PTC)的表达状态、临床价值及意义。方法选择手术完整切除的甲状腺乳头状癌40份、正常甲状腺组织40份、结节性甲状腺肿40份。采用免疫组织化学技术(Immunohistochemistry,I H C)检测组织中E Rβ2、 E Rα46表达情况,收集P T C患者术前淋巴结转移情况、临床分期及5年生存率。结果 E Rβ2、ERα46在甲状腺乳头状癌阳性表达率分别为92.5%、70.0%,2种受体在甲状腺乳头状癌和良性病变中的阳性表达差异均有统计学意义(P <0.05)。ERβ2、ERα46诊断乳头状癌的灵敏度分别为92.5%、70.0%,特异度分别为72.5%、78.8%;E Rβ2、 E Rα46的表达与甲状腺乳头状癌的临床分期及淋巴结转移有关,差异均有统计学意义(P <0. 05), 5年生存率、死亡患者ERβ2、ERα46阳性细胞率的差异无统计学意义(P>0.05)。结论 ERβ2、ERα46在甲状腺乳头状癌中表达增强,在病理诊断中有一定的价值,但对于判断远期预后的情况不明显。
基金This work was supported by grants from the National Natural Science Foundation of China （No. 30772510）, Ministry Health of the People＇s Republic of China （No. WKJ2006-2-008）, and Department of Science and Technology of Zhejiang Province （No. 2007C24011）.
文摘Background Estrogen is involved in suppression of colon cancer development and exerts its function via estrogen receptors α and β （ERa, ERβ）. The recently identified ERα46 resulted from exon 1-deletion from the 66-kDa full length form of ERa66 is devoid of the transactivation domain AF-1, whose function remains largely unknown. Methods In this study, we compared the expression of ERa46 mRNA in 32 normal colorectal tissues and their matched colorectal cancer tissues by real-time quantitative polymerase chain reaction （PCR）. Human colon adenocarcinoma cell HT-29, that has low endogenous expression of ERα46, was transfected with ERα46-expression vector; methyl thiazolyl tetrazolium （MTT） assay, flow cytometry, DNA fragmentation and TUNEL staining were used to evaluate the proliferation and apoptosis status of the cells in the presence of 17β-oestradiol. Results Higher ERα46 mRNA levels were observed in normal colorectal tissues than in the corresponding cancer tissues. ERα46-transfected cells showed a significantly decreased growth rate than control cells and an accumulation of cells in the G0/1 phase and a reduced proportion of cells in G2/M phase after exposed to 10^-8 mol/L 17β-oestradiol. There were also more positive TUNEL stained cells in ERα46-transfected cells than the control cells in the presence of 17β-oestradiol （P 〈0.05）. Conclusions These data suggest that ERα46 may be involved in the development and/or progression of colorectal cancer via mediating growth inhibition and apoptosis of cancer cells in the presence of 17β-oestradiol.
