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Leukocyte immunoglobulin-like receptor B2:A promising biomarker for colorectal cancer 被引量:1
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作者 Wen-Zhuo Zhao Hong-Gang Wang Xiao-Zhong Yang 《World Journal of Gastroenterology》 SCIE CAS 2024年第4期421-423,共3页
According to the latest global cancer statistics,colorectal cancer(CRC)has emerged as the third most prevalent malignant tumor across the globe.In recent decades,the medical field has implemented several levels of CRC... According to the latest global cancer statistics,colorectal cancer(CRC)has emerged as the third most prevalent malignant tumor across the globe.In recent decades,the medical field has implemented several levels of CRC screening tests,encompassing fecal tests,endoscopic examinations,radiological examinations and blood tests.Previous studies have shown that leukocyte immunoglobulin-like receptor B2(LILRB2)is involved in inhibiting immune cell function,immune evasion,and promoting tumor progression in acute myeloid leukemia and nonsmall cell lung cancer.However,its interaction with CRC has not been reported yet.Recently,a study published in the World Journal of Gastroenterology revealed that LILRB2 and its ligand,angiopoietin-like protein 2,are markedly overexpressed in CRC.This overexpression is closely linked to tumor progression and is indicative of a poor prognosis.The study highlights the potential of utilizing the concentration of LILRB2 in serum as a promising biomarker for tumors.However,there is still room for discussion regarding the data processing and analysis in this research. 展开更多
关键词 Colorectal cancer Leukocyte immunoglobulin-like receptor b2 Angiopoietinlike protein 2 Therapeutic target Noninvasive screening biomarker
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血清乳酸脱氢酶、β_(2)-微球蛋白及铁蛋白水平与侵袭性B细胞淋巴瘤患者预后的关系 被引量:1
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作者 彭湃 潘琼 闫晓倩 《癌症进展》 2024年第2期219-221,共3页
目的探讨血清乳酸脱氢酶(LDH)、β_(2)-微球蛋白(β_(2)-MG)及铁蛋白水平与侵袭性B细胞淋巴瘤患者预后的关系。方法选取60例侵袭性B细胞淋巴瘤患者,均接受利妥昔单抗+环磷酰胺+表柔比星+长春新碱+泼尼松(R-CHOP)方案化疗,治疗4个疗程后... 目的探讨血清乳酸脱氢酶(LDH)、β_(2)-微球蛋白(β_(2)-MG)及铁蛋白水平与侵袭性B细胞淋巴瘤患者预后的关系。方法选取60例侵袭性B细胞淋巴瘤患者,均接受利妥昔单抗+环磷酰胺+表柔比星+长春新碱+泼尼松(R-CHOP)方案化疗,治疗4个疗程后评估临床疗效,检测治疗前后患者血清LDH、β_(2)-MG、铁蛋白水平。随访3年,比较不同预后侵袭性B细胞淋巴瘤患者治疗4个疗程后血清LDH、β_(2)-MG、铁蛋白水平,其与患者预后的相关性采用Spearman秩相关分析。结果60例侵袭性B细胞淋巴瘤患者化疗4个疗程后,完全缓解38例,部分缓解7例,疾病稳定4例,疾病进展11例,疾病控制率为81.67%(49/60)。治疗4个疗程后,侵袭性B细胞淋巴瘤患者血清LDH、β_(2)-MG、铁蛋白水平均明显低于治疗前,差异均有统计学意义(P﹤0.01)。随访3年,所有患者均无失访,生存43例,死亡17例。生存患者治疗4个疗程后血清LDH、β_(2)-MG、铁蛋白水平均明显低于死亡患者,差异均有统计学意义(P﹤0.01)。Spearman秩相关分析结果显示,血清LDH、β_(2)-MG、铁蛋白水平与侵袭性B细胞淋巴瘤患者的预后生存均呈负相关(r=-0.718、-0.823、-0.753,P﹤0.05)。结论血清LDH、β_(2)-MG、铁蛋白水平与侵袭性B细胞淋巴瘤患者的预后生存均呈负相关,有助于临床对患者的预后进行客观评价。 展开更多
关键词 侵袭性b细胞淋巴瘤 R-CHOP β_(2)-微球蛋白
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Serotonin receptor 2B induces visceral hyperalgesia in rat model and patients with diarrhea-predominant irritable bowel syndrome
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作者 Zheng-Yang Li Yu-Qing Mao +6 位作者 Qian Hua Yong-Hong Sun Hai-Yan Wang Xuan-Guang Ye Jing-Xian Hu Ya-Jie Wang Miao Jiang 《World Journal of Gastroenterology》 SCIE CAS 2024年第10期1431-1449,共19页
BACKGROUND Serotonin receptor 2B(5-HT2B receptor)plays a critical role in many chronic pain conditions.The possible involvement of the 5-HT2B receptor in the altered gut sensation of irritable bowel syndrome with diar... BACKGROUND Serotonin receptor 2B(5-HT2B receptor)plays a critical role in many chronic pain conditions.The possible involvement of the 5-HT2B receptor in the altered gut sensation of irritable bowel syndrome with diarrhea(IBS-D)was investigated in the present study.AIM To investigate the possible involvement of 5-HT2B receptor in the altered gut sensation in rat model and patients with IBS-D.METHODS Rectosigmoid biopsies were collected from 18 patients with IBS-D and 10 patients with irritable bowel syndrome with constipation who fulfilled the Rome IV criteria and 15 healthy controls.The expression level of the 5-HT2B receptor in colon tissue was measured using an enzyme-linked immunosorbent assay and correlated with abdominal pain scores.The IBS-D rat model was induced by intracolonic instillation of acetic acid and wrap restraint.Alterations in visceral sensitivity and 5-HT2B receptor and transient receptor potential vanilloid type 1(TRPV1)expression were examined following 5-HT2B receptor antagonist adminis-tration.Changes in visceral sensitivity after administration of the TRPV1 antago-INTRODUCTION Irritable bowel syndrome(IBS)is a chronic functional bowel disorder characterized by recurrent abdominal pain with altered bowel habits that affects approximately 15%of the population worldwide[1].IBS significantly impacts the quality of life of patients.Although the pathogenesis of IBS is not completely understood,the role of abnormal visceral sensitivity in IBS has recently emerged[2,3].5-Hydroxytryptamine(5-HT)is known to play a key role in the physiological states of the gastrointestinal tract.Plasma 5-HT levels in IBS with diarrhea(IBS-D)patients were greater than those in healthy controls[4],suggesting a possible role of 5-HT in the pathogenesis of IBS-D.The serotonin receptor 2(5-HT2 receptor)family comprises three subtypes:5-HT2A,5-HT2B,and 5-HT2c.All 5-HT2 receptors exhibit 46%-50%overall sequence identity,and all of these receptors preferentially bind to Gq/11 to increase inositol phosphates and intracellular calcium mobilization[5].5-HT2B receptors are widely expressed throughout the gut,and experimental evidence suggests that the primary function of 5-HT2B receptors is to mediate contractile responses to 5-HT through its action on smooth muscle[6].The 5-HT2B receptor is localized to both neurons of the myenteric nerve plexus and smooth muscle in the human colon.The 5-HT2B receptor mediates 5-HT-evoked contraction of longitudinal smooth muscle[6].These findings suggest that the 5-HT2B receptor could play an important role in modulating colonic motility,which could affect sensory signaling in the gut.Other laboratories have shown that the 5-HT2B receptor participates in the development of mechanical and formalin-induced hyperalgesia[7,8].A 5-HT2B receptor antagonist reduced 2,4,6-trinitrobenzene sulfonic acid(TNBS)and stress-induced visceral hyperalgesia in rats[9,10].However,the role of the 5-HT2B receptor in IBS-D patients and in acetic acid-and wrap restraint-induced IBS-D rat models was not investigated. 展开更多
关键词 Diarrhea-predominant irritable bowel syndrome Serotonin receptor 2b Transient receptor potential vanilloid type-1 Visceral hypersensitivity Abdominal pain
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Down-regulation of pancreatic transcription factors and incretin receptors in type 2 diabetes 被引量:9
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作者 Hideaki Kaneto Taka-aki Matsuoka 《World Journal of Diabetes》 SCIE CAS 2013年第6期263-269,共7页
Type 2 diabetes is one of the most prevalent and serious metabolic diseases.Under diabetic conditions,chronic hyperglycemia and subsequent induction of oxidative stress deteriorate pancreaticβ-cell function,which lea... Type 2 diabetes is one of the most prevalent and serious metabolic diseases.Under diabetic conditions,chronic hyperglycemia and subsequent induction of oxidative stress deteriorate pancreaticβ-cell function,which leads to the aggravation of type 2 diabetes.Although such phenomena are well known as glucose toxicity,its molecular mechanism remains unclear.In this review article,we describe the possible molecular mechanism forβ-cell dysfunction found in type 2 diabetes,focusing on(1)oxidative stress,(2)pancreatic transcription factors(PDX-1 and MafA)and(3)incretin receptors(GLP-1 and GIP receptors).Under such conditions,nuclear expression levels of PDX-1 and MafA are decreased,which leads to suppression of insulin biosynthesis and secretion.In addition,expression levels of GLP-1 and GIP receptors are decreased,which likely contributes to the impaired incretin effects found in diabetes.Taken together,it is likely that downregulation of pancreatic transcription factors(PDX-1and MafA)and down-regulation of incretin receptors(GLP-1 and GIP receptors)explain,at least in part,the molecular mechanism forβ-cell dysfunction found in type 2 diabetes. 展开更多
关键词 Pancreatic b2 -CELLS Oxidative stress Pancreatic duodenal homeobox-1 MAFA Incretin receptor
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Down-regulation of Cardiac Bradykinin B2 Receptors and eNOs mRNA in Rats with Remnant Kidneys
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作者 万槐斌 涂玲 +1 位作者 刘晓晴 邓娟娟 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2005年第3期276-278,共3页
The changes in the expression of cardiac bradykinin B2 receptors (BKB2Rs) and endogenous nitrix oxide synthase (eNOs) mRNA were studied in rats with remnant kidneys. Thirty-two rats were divided into sham-operated and... The changes in the expression of cardiac bradykinin B2 receptors (BKB2Rs) and endogenous nitrix oxide synthase (eNOs) mRNA were studied in rats with remnant kidneys. Thirty-two rats were divided into sham-operated and experimental groups randomly (n=16 in each group). The remnant kidney model was established by 2-stage 5/6 nephrectomy. Blood pressure and serum Cr were measured before operation and 15, 30, 60, 120 days after 5/6 nephrectomy. Eight animals in each group were killed at the first month and 4th month after the operation. The expression of BKB2Rs and eNOs mRNAs was detected by using RT-real time PCR from isolated left ventricle, and their correlation was also analyzed. The results showed that blood pressure and serum Cr were increased significantly 15 days after 5/6 nephrectomy (both P<0.01), and the hypertension and azomia existed constantly till 120 days but had no significant fluctuation. Cardiac BKB2Rs and eNOs mRNA was declined time-dependently (both P<0.05). And there was a close positive correlation between cardiac BKB2Rs and eNOs mRNA (r=0.82, P<0.01). It was suggested that a significant chronic renal failure can be produced at least 15 days after 5/6 nephrotomy and can sustain more than 4 months. The expression of BKB2Rs and eNOs was down-regulated time-dependently in this model, and there was a significant correlation between them. 展开更多
关键词 bradykinin b2 receptors endogenous nitric oxide synthase MYOCARDIUM 5/6 nephretomy
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血清β_(2)-MG联合LDH在弥漫性大B细胞淋巴瘤预后判断中的意义 被引量:11
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作者 郑永青 孙明东 +2 位作者 王瑞芳 王立盼 李砚如 《疑难病杂志》 CAS 2022年第6期582-587,共6页
目的探究血清β_(2)-微球蛋白(β_(2)-MG)联合乳酸脱氢酶(LDH)在弥漫性大B细胞淋巴瘤(DLBCL)患者中的预后价值。方法选择2011年1月—2018年12月山东省滨州市人民医院血液科收治DLBCL患者82例作为DLBCL组,并根据随访3年生存情况分为生存... 目的探究血清β_(2)-微球蛋白(β_(2)-MG)联合乳酸脱氢酶(LDH)在弥漫性大B细胞淋巴瘤(DLBCL)患者中的预后价值。方法选择2011年1月—2018年12月山东省滨州市人民医院血液科收治DLBCL患者82例作为DLBCL组,并根据随访3年生存情况分为生存亚组46例和死亡亚组36例,另选同期于医院治疗的淋巴结反应增生(RLNH)患者43例作为RLNH组。比较2组患者血清β_(2)-MG、LDH水平;Pearson检验分析DLBCL患者血清β_(2)-MG与LDH的相关性;比较血清β_(2)-MG、LDH水平在DLBCL患者不同临床病理特征中的差异;Kaplan-Meier法绘制生存曲线分析血清β_(2)-MG、LDH水平及二者联合与DLBCL患者预后的关系;多因素Logistic回归分析影响DLBCL患者预后的危险因素;受试者工作特征曲线(ROC)分析血清β_(2)-MG、LDH及二者联合对DLBCL患者预后的预测价值。结果DLBCL组血清β_(2)-MG、LDH水平明显高于RLNH组(t/P=6.941/<0.001、6.980/<0.001),DLBCL患者血清β_(2)-MG与LDH呈明显正相关(r/P=0.553/<0.001);DLBCL患者血清β_(2)-MG、LDH水平分别与临床分期、国际预后指数(IPI)评分、美国东部协作肿瘤组体质状态(ECOG PS)评分有关,而LDH还与病理类型有关(P均<0.05);血清β_(2)-MG高表达患者3年生存率明显低于低表达患者(43.90%vs.68.29%,χ^(2)/P=4.952/0.026),血清LDH高表达患者3年生存率明显低于低表达患者(39.02%vs.73.17%,χ^(2)/P=9.705/0.002);死亡亚组临床分期Ⅲ~Ⅳ期、有B症状、IPI评分3~4分、ECOG PS评分>2分、淋巴细胞计数降低、β_(2)-MG高表达、LDH高表达比例明显高于生存亚组(P<0.05)。临床分期Ⅲ~Ⅳ期、有B症状、IPI评分3~4分、β_(2)-MG高表达、LDH高表达是导致DLBCL患者预后不良的独立危险因素[OR(95%CI)=3.864(3.436~4.402)、3.587(3.124~4.086)、4.247(3.724~4.943)、4.165(3.763~4.716)、4.032(3.521~4.482)];血清β_(2)-MG、LDH及二者联合预测DLBCL的ROC曲线下面积分别为0.642、0.775和0.852,二者联合的AUC高于单独预测价值(Z/P=9.421/<0.001、8.246/<0.001)。结论DLBCL患者血清β_(2)-MG、LDH高表达,二者联合在DLBCL患者预后中具有一定的预测价值。 展开更多
关键词 弥漫性大b细胞淋巴瘤 β_(2)-微球蛋白
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氯胺酮多次给药对大鼠肝微粒体内细胞色素P450_2B酶的诱导作用 被引量:7
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作者 代晶 杨万清 廖林川 《成都医学院学报》 CAS 2012年第3期383-385,共3页
目的考察氯胺酮对大鼠肝微粒体内细胞色素P450_2B酶活性的影响。方法以10mg.kg-1.d-1氯胺酮灌胃给予大鼠,连续给药10d。在第11d处死大鼠,以钙离子沉淀法制备肝微粒体。采用Omura和Sato方法测定细胞色素P450含量,采用7-戊氧基试卤灵-O-... 目的考察氯胺酮对大鼠肝微粒体内细胞色素P450_2B酶活性的影响。方法以10mg.kg-1.d-1氯胺酮灌胃给予大鼠,连续给药10d。在第11d处死大鼠,以钙离子沉淀法制备肝微粒体。采用Omura和Sato方法测定细胞色素P450含量,采用7-戊氧基试卤灵-O-去烷基反应测定细胞色素P450_2B酶的活性。结果给药组和对照组细胞色素P450的总含量分别是(0.679±0.216)nmol·mg-1和(0.398±0.109)nmol·mg-1,细胞色素P450_2B酶的活性分别是(13.40±3.15)pmol·min-1.mg-1和(7.04±2.09)pmol·min-1.mg-1。结论氯胺酮能够增加大鼠肝微粒中细胞色素P450含量,并能显著诱导细胞色素P450_2B酶的活性。 展开更多
关键词 P450 细胞色素P450_2b
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PCNA、Bcl-2及EGFR在喉癌组织中的表达及与临床病理特征、生存的关系 被引量:1
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作者 黄海平 李佳宸 《中国现代医学杂志》 CAS 2024年第2期76-82,共7页
目的探讨增殖细胞核抗原(PCNA)、B淋巴细胞瘤-2(Bcl-2)及表皮生长因子受体(EGFR)在喉癌组织中的表达及与临床病理特征、生存的关系。方法选取2017年3月—2020年1月在苏州大学附属第一医院因喉癌行手术治疗的92例患者的喉癌组织及对应癌... 目的探讨增殖细胞核抗原(PCNA)、B淋巴细胞瘤-2(Bcl-2)及表皮生长因子受体(EGFR)在喉癌组织中的表达及与临床病理特征、生存的关系。方法选取2017年3月—2020年1月在苏州大学附属第一医院因喉癌行手术治疗的92例患者的喉癌组织及对应癌旁组织标本。检测癌组织与癌旁组织PCNA mRNA、Bcl-2mRNA、EGFR mRNA相对表达量,多元线性回归分析其癌组织表达与临床病理特征的关系。随访3年,采用Kaplain-Maier曲线分析不同PCNA、Bcl-2、EGFR表达水平患者生存情况差异。结果癌组织PCNA mRNA、Bcl-2 mRNA、EGFR mRNA相对表达量高于癌旁组织(P<0.05)。不同年龄、肿瘤部位患者PCNA mRNA、Bcl-2 mRNA、EGFR mRNA相对表达量比较,差异无统计学意义(P>0.05);低分化,临床分期Ⅲ、Ⅳ期及淋巴结转移患者PCNA mRNA、Bcl-2 mRNA、EGFR mRNA相对表达量分别高于中、高分化,临床分期Ⅰ、Ⅱ期,无淋巴结转移患者(P<0.05)。多元线性回归分析结果显示,肿瘤分化程度、临床分期、淋巴结转移是喉癌组织PCNA mRNA、Bcl-2 mRNA、EGFR mRNA表达的影响因素。Kaplain-Maier曲线分析结果显示,PCNA mRNA高表达患者3年无进展生存率、总生存率分别为59.57%和70.21%,低于低表达患者的80.00%和88.89%(P<0.05);Bcl-2 mRNA高表达患者3年无进展生存率、总生存率分别为60.78%和70.59%,低于低表达患者的80.49%和90.24%(P<0.05);EGFR mRNA高表达患者3年无进展生存率、总生存率分别为59.09%和70.45%,低于低表达患者的79.17%、87.50%(P<0.05)。结论喉癌组织PCNA、Bcl-2、EGFR呈高表达,且其高表达状态与肿瘤分期高、分化程度低、淋巴结转移有关,PCNA、Bcl-2、EGFR表达水平可在一定程度上反映患者预后。 展开更多
关键词 b淋巴细胞瘤-2
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姜黄素提取物对OSF模型大鼠TGF-β_(1)、IL-1和Bcl-2的影响及相关机制探讨 被引量:5
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作者 张琳 谭劲 +4 位作者 刘一平 刘寻 周婷婷 禹洁 黄笑薇 《疑难病杂志》 CAS 2021年第11期1152-1156,共5页
目的探讨姜黄素提取物对口腔黏膜下纤维化(OSF)模型大鼠转化生长因子β_(1)(TGF-β_(1))、白介素1(IL-1)和B淋巴细胞瘤2(Bcl-2)的影响及相关机制。方法2021年3—6月于湖南中医药大学第一附属医院动物研究中心进行实验。选取清洁SPF级大... 目的探讨姜黄素提取物对口腔黏膜下纤维化(OSF)模型大鼠转化生长因子β_(1)(TGF-β_(1))、白介素1(IL-1)和B淋巴细胞瘤2(Bcl-2)的影响及相关机制。方法2021年3—6月于湖南中医药大学第一附属医院动物研究中心进行实验。选取清洁SPF级大鼠60只,随机数字表法选取15只为正常对照组,其余大鼠建立OSF模型,成功42只,随机分成模型组、阳性对照组和姜黄素提取物组,各14只。阳性对照组大鼠皮下注射曲安奈德,姜黄素提取物组大鼠给予姜黄素提取物灌胃,正常对照组和模型组大鼠给予等体积生理盐水灌胃。干预8周后,观察各组大鼠口腔黏膜病理组织学变化,比较开口度情况,检测TGF-β_(1)、IL-1和Bcl-2蛋白表达量及Wnt信号通路因子表达量。结果与模型组比较,阳性对照组、姜黄素提取物组大鼠实验第1、3个月张口度增大(P<0.05),且姜黄素提取物组大于阳性对照组(P<0.05)。与模型组比较,阳性对照组、姜黄素提取物组血清TGF-β_(1)、IL-1、Bcl-2蛋白表达量降低(P<0.05),且姜黄素提取物组低于阳性对照组(P<0.05)。与模型组比较,阳性对照组、姜黄素提取物组β-catenin、APC、Bcl-2 mRNA表达量均降低(P<0.05),且姜黄素提取物组低于阳性对照组(P<0.05)。结论姜黄素提取物可能通过作用于Wnt信号通路,调控β-catenin、APC、Bcl-2 mRNA表达量,降低TGF-β_(1)、IL-1、Bcl-2水平,改善纤维化情况。 展开更多
关键词 β_(1) 1 b淋巴细胞瘤2 Wnt
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EGFR/HER_1和C-erb B-2/HER_2在乳腺癌表达的意义及其相关性 被引量:4
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作者 芦广萍 田亚汀 闫爱春 《现代肿瘤医学》 CAS 2008年第9期1520-1521,共2页
目的:探讨乳腺癌中EGFR和C-erb B-2基因蛋白表达的临床意义。方法:乳腺癌86例,乳腺腺瘤36例,采用免疫组织化学方法,检测EGFR/HER1和C-erb B-2/HER2阳性率,分析它们的相关性。结果:EGFR和C-erb B-2在乳腺癌中的表达高度正相关(r=0.73)。... 目的:探讨乳腺癌中EGFR和C-erb B-2基因蛋白表达的临床意义。方法:乳腺癌86例,乳腺腺瘤36例,采用免疫组织化学方法,检测EGFR/HER1和C-erb B-2/HER2阳性率,分析它们的相关性。结果:EGFR和C-erb B-2在乳腺癌中的表达高度正相关(r=0.73)。它们在乳腺癌中的阳性率明显高于腺瘤(P<0.001)。在乳腺癌中EGFR和C-erb B-2的阳性率分别为56.98%(49/86)和70.93%(61/86),差异无显著性(P>0.05)。在浸润癌和有淋巴结转移组阳性率高于非浸润组和无淋巴结转移组(P<0.001)。结论:EGFR和C-erbB-2在乳腺癌发生中有协同作用,是预后不良的指标。 展开更多
关键词 C—erb b-2
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TRPM7和BTG2在宫颈癌组织中的表达及临床意义
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作者 周立飞 高跃丽 +4 位作者 耿欣 张静亚 耿飞龙 康非 王亚凡 《中国性科学》 2024年第10期100-105,共6页
目的探讨瞬时受体电位M7通道(TRPM7)和B细胞迁移基因2(BTG2)在宫颈癌组织中的表达及临床意义。方法选取2018年5月至2020年5月石家庄市妇幼保健院收治的110例宫颈癌患者作为研究对象,术中取其肿瘤组织及癌旁组织,根据随访情况分为预后良... 目的探讨瞬时受体电位M7通道(TRPM7)和B细胞迁移基因2(BTG2)在宫颈癌组织中的表达及临床意义。方法选取2018年5月至2020年5月石家庄市妇幼保健院收治的110例宫颈癌患者作为研究对象,术中取其肿瘤组织及癌旁组织,根据随访情况分为预后良好组与预后不良组。采用免疫组化法检测癌旁组织和肿瘤组织中TRPM7、BTG2蛋白表达,分析TRPM7、BTG2蛋白表达水平与患者临床病理特征的关系,比较预后不良组与预后良好组肿瘤组织中TRPM7、BTG2蛋白表达,采用Cox回归分析宫颈癌患者预后的影响因素,采用Kaplan-Meier曲线分析TRPM7、BTG2水平与宫颈癌患者预后的关系。结果与癌旁组织比较,肿瘤组织中TRPM7蛋白表达水平升高,BTG2蛋白表达水平降低(P<0.05)。TRPM7、BTG2蛋白表达水平与肿瘤分化程度、淋巴结转移、恶性肿瘤国际临床病理(TNM)分期有关(P<0.05)。与预后良好组比较,预后不良组肿瘤组织中TRPM7蛋白表达水平升高,BTG2蛋白表达水平降低(P<0.05)。TRPM7、TNM分期(Ⅲ期)、肿瘤低分化程度、淋巴结转移是宫颈癌患者预后的危险因素(P<0.05),BTG2是宫颈癌患者预后的保护因素(P<0.05)。TRPM7高表达组3年生存率低于TRPM7低表达组(P<0.05);BTG2高表达组3年生存率高于BTG2低表达组(P<0.05)。结论宫颈癌患者肿瘤组织中TRPM7蛋白表达上调,BTG2蛋白表达下调,均与宫颈癌预后有关。 展开更多
关键词 M7 b细胞迁移基因2
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血清高迁移率族蛋白B1、β_(2)-微球蛋白、乳酸脱氢酶及白细胞介素-6在多发性骨髓瘤中的表达及相关性 被引量:1
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作者 田颖 王根杰 +1 位作者 胡青竹 杨硕 《癌症进展》 2023年第22期2529-2532,共4页
目的 探讨血清高迁移率族蛋白B1(HMGB1)、β_(2)-微球蛋白(β_(2)-MG)、乳酸脱氢酶(LDH)及白细胞介素-6(IL-6)在多发性骨髓瘤中的表达及相关性。方法 选取60例健康体检者和86例多发性骨髓瘤患者,其中31例初诊未治,55例接受硼替佐米+地... 目的 探讨血清高迁移率族蛋白B1(HMGB1)、β_(2)-微球蛋白(β_(2)-MG)、乳酸脱氢酶(LDH)及白细胞介素-6(IL-6)在多发性骨髓瘤中的表达及相关性。方法 选取60例健康体检者和86例多发性骨髓瘤患者,其中31例初诊未治,55例接受硼替佐米+地塞米松+沙利度胺(BDT)方案化疗。比较多发性骨髓瘤患者与体检者、初诊未治与BDT方案治疗不同临床疗效患者、不同临床分期初诊未治多发性骨髓瘤患者血清HMGB1、β_(2)-MG、LDH、IL-6水平。血清HMGB1水平与β_(2)-MG、LDH、IL-6水平的相关性采用Pearson相关分析。结果 多发性骨髓瘤患者血清HMGB1、β_(2)-MG、LDH及IL-6水平均明显高于体检者,差异均有统计学意义(P﹤0.01)。初诊未治及无效患者血清HMGB1、β_(2)-MG、LDH、IL-6水平均明显高于有效患者,无效患者血清HMGB1、β_(2)-MG、LDH、IL-6水平均明显高于初诊未治患者,差异均有统计学意义(P﹤0.01)。临床分期为Ⅱ、Ⅲ期的初诊未治多发性骨髓瘤患者血清HMGB1、β_(2)-MG、LDH、IL-6水平均明显高于Ⅰ期患者,Ⅲ期患者的血清HMGB1、β_(2)-MG、LDH、IL-6水平均明显高于Ⅱ期患者,差异均有统计学意义(P﹤0.01)。Pearson相关分析显示,血清HMGB1水平与β_(2)-MG、LDH、IL-6水平均呈正相关(r=0.902、0.867、0.882,P﹤0.05)。结论 多发性骨髓瘤患者血清HMGB1水平较高,BDT方案治疗后可使其显著降低,且与β_(2)-MG、LDH、IL-6水平均呈正相关,临床可联合HMGB1、β_(2)-MG、LDH、IL-6共同作为评估多发性骨髓瘤疾病严重程度和治疗效果的分子靶点。 展开更多
关键词 高迁移率族蛋白b1 β_(2)-微球蛋白 -6
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1,25(OH)_(2)D_(3)对miR-130b转染的高糖条件下HMC细胞TGF-β1/Smad3及Col-Ⅳ表达的影响 被引量:1
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作者 王琴 高倩 +2 位作者 廖静 沈呈 杨烨 《四川医学》 CAS 2021年第6期541-546,共6页
目的观察高糖环境下1,25(OH)_(2)D_(3)对miR-130b转染肾小球系膜细胞TGF-β1/Smad3、Col-IV表达水平的变化,探索1,25(OH)_(2)D_(3)是否可通过miR-130b改善糖尿病肾病肾脏纤维化的过程。方法HMC细胞传代培养,miR-130b mimics及miR-130b i... 目的观察高糖环境下1,25(OH)_(2)D_(3)对miR-130b转染肾小球系膜细胞TGF-β1/Smad3、Col-IV表达水平的变化,探索1,25(OH)_(2)D_(3)是否可通过miR-130b改善糖尿病肾病肾脏纤维化的过程。方法HMC细胞传代培养,miR-130b mimics及miR-130b inhibitor经LipofectamineTM 2000转染肾小球系膜细胞,依据分组加入1,25(OH)_(2)D_(3)1.0×10^(-8) mol/L,37℃、5%CO_(2)饱和湿度条件下继续培养48 h,共分为6组:①空白对照组;②细胞+无水乙醇组(A组);③细胞+miR-130b mimics+无水乙醇组(B组);④细胞+miR-130b mimics+1,25(OH)_(2)D_(3)组(C组);⑤细胞+miR-130b inhibitor+无水乙醇组(D组);⑥细胞+miR-130b inhibitor+1,25(OH)_(2)D_(3)组(E组)。以实时荧光定量PCR检测细胞miR-130b及TGF-β1、Smad3、Col-IVmRNA的表达水平,以Western blot方法检测细胞TGF-β1、Smad3、Col-IV蛋白的表达水平。采用多组间比较的单因素方差分析及组内两两比较采用LSD、Dunnett s T3法比较各组数据。结果空白对照组与A组细胞miR-130b、TGF-β1、Smad3、Col-IV表达水平,差异无统计学意义(P>0.05),与A组相比,B组miR-130b、TGF-β1、Smad3、Col-IV表达水平明显升高(P<0.05),D组miR-130b、TGF-β1、Smad3、Col-IV表达水平明显降低(P<0.05)。经1,25(OH)_(2)D_(3)治疗发现,与B组相比,C组miR-130b、TGF-β1、Smad3、Col-IV表达水平明显降低(P<0.05),与D组相比,E组miR-130b、TGF-β1、Smad3、Col-IV表达水平明显降低(P<0.05)。结论1,25(OH)_(2)D_(3)可降低miR-130b转染肾小球系膜细胞miR-130b及纤维化相关因子TGF-β1、Smad3、Col-IV的表达水平,1,25(OH)_(2)D_(3)可能通过miR-130b改善糖尿病肾病肾脏纤维化的过程。 展开更多
关键词 1 25(OH)_(2)D_(3) HMC miR-130b转染 TGF-β1/Smad3 Col-
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SNP Identification in α_(2A)-Adrenergic Receptor Gene in Chinese and the Effect on Gene Expression
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作者 袁栎 沈士弼 罗超权 《Journal of Nanjing Medical University》 2003年第6期277-282,共6页
Objective: To scan single nucleotide polymorphism ( SNP ) in Chinese alpha-2Aadrenergic receptor (α_(2A)-AR) gene and study the effects of the SNP on the gene expression.Methods: The complete sequence of α_(2A)-AR g... Objective: To scan single nucleotide polymorphism ( SNP ) in Chinese alpha-2Aadrenergic receptor (α_(2A)-AR) gene and study the effects of the SNP on the gene expression.Methods: The complete sequence of α_(2A)-AR gene was analyzed with automated DNA sequencer to scanSNPs. Genomic DNA was extracted from whole blood and a 239 bp fragment containing the G/Cpolymorphism was amplified with PCR using a pair of. specific primers. PCR-RFLP was used to performthe genotyping of the SNP at the site-1 296 bp of the people in the North of China. Electrophoresismobility shift assay ( EMSA ) was used to study the binding of the 390 bp fragments (- 1 414-1 025bp) with G or C at the site-1 296 bp and nuclear extracts . Results: In our study, two SNPs werefound in α_(2A)-AR gene. Allele frequencies of the SNP at the site-1 296 bp were 0.61 and 0.39 forG and C , and the genotype frequencies were 0.34 , 0.54 and 0.13 for GG, GC and CC respectively fromthe people in the North of China. In the EMSA, a specific binding appeared in the complex ofnuclear extracts and DNA with C at-1 296 bp . Conclusion: Two SNPs exist in α_(2A)-AR gene from thepeople in the North of China , and DNA fragment with allele C of the SNP at the site-1 296 bp couldbind with a specific protein, which could influence the gene expression. 展开更多
关键词 α_(2A)-adrenergic receptor single nucleotide polymorphism gene expression
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慢性乙型肝炎患者HBV DNA载量及血清IL-2R、GP73、miR-21与HBVM表达模式、肝组织病理学改变的相关性 被引量:1
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作者 李述美 刘冰 +3 位作者 彭思璐 刘仁伟 李铃 孙宏 《临床和实验医学杂志》 2024年第10期1017-1021,共5页
目的探讨慢性乙型肝炎患者乙型肝炎病毒(HBV)DNA载量及血清白细胞介素-2受体(IL-2R)、高尔基体蛋白73(GP73)、微小RNA-21(miR-21)与乙型肝炎病毒血清标志物(HBVM)表达模式、肝组织病理学改变的相关性。方法回顾性选取2020年1月至2022年1... 目的探讨慢性乙型肝炎患者乙型肝炎病毒(HBV)DNA载量及血清白细胞介素-2受体(IL-2R)、高尔基体蛋白73(GP73)、微小RNA-21(miR-21)与乙型肝炎病毒血清标志物(HBVM)表达模式、肝组织病理学改变的相关性。方法回顾性选取2020年1月至2022年12月四川绵阳四0四医院(绵阳市第一人民医院)收治的106例慢性乙型肝炎患者作为观察组,另纳入本院125名同期健康体检者为对照组。观察组根据肝脏炎症程度分为炎症较轻组(G_(0)~G_(2)期,n=61)和炎症较重组(G_(3)~G_(4)期,n=45);根据肝纤维化程度分为纤维化较轻组(S_(0)~S_(2)期,n=80)和纤维化较重组(S_(3)~S_(4)期,n=26);根据外周血中是否产生乙肝表面抗体(抗-HBs)分为抗-HBs阳性组(n=77)和抗-HBs阴性组(n=29)。比较观察组与对照组血清IL-2R、GP73、miR-21水平,比较不同肝脏炎症程度、不同肝脏纤维化程度、不同HBVM表达模式的慢性乙型肝炎患者HBV DNA载量、血清IL-2R、GP73、miR-21水平,并采用Spearman相关性分析分析HBV DNA载量、血清IL-2R、GP73、miR-21与肝组织病理学改变的相关性。结果观察组血清IL-2R、GP73、miR-21水平分别为(559.65±46.65)U/mL、(149.87±31.65)ng/mL、1.98±0.65,均高于对照组[(272.54±28.65)U/mL、(48.76±9.65)ng/mL、1.23±0.36],差异均有统计学意义(P<0.05)。炎症较重组HBV DNA载量、血清IL-2R、GP73、miR-21水平分别为(5.98±0.73)loads、(703.54±57.54)U/mL、(243.65±38.65)ng/mL、1.99±0.54,均高于炎症较轻组[(4.65±0.68)loads、(450.65±36.65)U/mL、(80.43±20.34)ng/mL、1.54±0.45],差异均有统计学意义(P<0.05)。纤维化较重组HBV DNA载量、血清IL-2R、GP73、miR-21水平分别为(6.03±0.98)loads、(675.65±58.04)U/mL、(189.65±41.65)ng/mL、2.34±0.34,均高于纤维化较轻组[(4.48±0.79)loads、(522.65±31.54)U/mL、(135.76±26.54)ng/mL、1.31±0.28],差异均有统计学意义(P<0.05)。抗-HBs阳性组和抗-HBs阴性组HBV DNA载量、血清IL-2R、GP73、miR-21水平比较,差异均无统计学意义(P>0.05)。HBV DNA载量、血清IL-2R、GP73、miR-21均与肝脏炎症程度呈正比(r=0.546、0.498、0.657、0.508,P<0.05),与肝脏纤维化程度呈正比(r=0.487、0.506、0.673、0.703,P<0.05)。结论慢性乙型肝炎患者血清中IL-2R、GP73、miR-21表达较高,且患者HBV DNA载量、血清IL-2R、GP73、miR-21水平与肝脏炎症及纤维化程度呈正比,但其与HBVM表达模式无明显相关性。 展开更多
关键词 白细胞介素-2受体 73 微小RNA-21
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1,25(OH)_(2)D_(3)联合NF-κB p65 siRNA保护肺炎链球菌感染的肺泡上皮细胞损伤 被引量:4
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作者 代雯文 妥亚军 +1 位作者 侯滨 王梁 《中国免疫学杂志》 CAS CSCD 北大核心 2021年第23期2838-2844,共7页
目的:探讨1,25(OH)_(2)D_(3)与核转录因子-κB(NF-κB)p65 siRNA单独或联合作用对肺炎链球菌(SP)感染的肺泡上皮细胞增殖、凋亡的影响。方法:分别使用1,25(OH)_(2)D_(3)、NF-κB p65 siRNA及二者联合处理SP感染的肺泡上皮细胞A549,实时... 目的:探讨1,25(OH)_(2)D_(3)与核转录因子-κB(NF-κB)p65 siRNA单独或联合作用对肺炎链球菌(SP)感染的肺泡上皮细胞增殖、凋亡的影响。方法:分别使用1,25(OH)_(2)D_(3)、NF-κB p65 siRNA及二者联合处理SP感染的肺泡上皮细胞A549,实时荧光定量PCR(qRT-PCR)检测NF-κB p65 mRNA表达,Western blot检测NF-κB p65、Bcl2相关X蛋白(Bax)、B细胞淋巴瘤/白血病2(Bcl2)、IL-1β、IL-6、TNF-α、NF-κB p-p65蛋白表达,MTT比色法和流式细胞术分别检测细胞活力与凋亡。结果:SP明显促进A549细胞中NF-κB p65 mRNA、NF-κB p65蛋白表达、细胞凋亡率、Bax、IL-1β、IL-6、TNF-α、NF-κB p-p65表达(P<0.05),显著抑制相对细胞活力、Bcl2表达(P<0.05)。1,25(OH)_(2)D_(3)、NF-κB p65 siRNA以及1,25(OH)_(2)D_(3)联合NF-κB p65 siRNA处理均明显抑制SP感染的A549细胞中NF-κB p65 mRNA、NF-κB p65蛋白表达、细胞凋亡、Bax、IL-1β、IL-6、TNF-α、NF-κB p-p65表达(P<0.05),提高细胞活力、Bcl2表达(P<0.05),并且1,25(OH)_(2)D_(3)联合NF-κB p65 siRNA对SP损伤A549细胞增殖、抑制细胞凋亡的作用更强。结论:1,25(OH)_(2)D_(3)联合NF-κB p65 siRNA对SP感染的肺泡上皮细胞损伤具有保护作用,且效果优于1,25(OH)_(2)D_(3)或NF-κB p65 siRNA单独作用,机制可能与调控炎症因子、NF-κB信号通路有关。 展开更多
关键词 1 25(OH)_(2)D_(3) NF-κb p65
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ZrO_(2)-(NH_(4))_(3)PW_(12)O_(40)光催化降解罗丹明B 被引量:2
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作者 冯少凡 李征 +3 位作者 李芳 郝泽坤 胡乐 李静 《化工环保》 CAS CSCD 北大核心 2022年第2期225-230,共6页
采用水热沉淀法制备了ZrO_(2)-(NH_(4))_(3)PW_(12)O_(40)复合光催化剂。采用XRD、SEM、TEM、UV-Vis DRS和FTIR等技术对催化剂进行了表征,并通过光催化降解罗丹明B(RhB)评价其光催化性能。实验结果表明:ZrO_(2)与(NH_(4))_(3)PW_(12)O_(... 采用水热沉淀法制备了ZrO_(2)-(NH_(4))_(3)PW_(12)O_(40)复合光催化剂。采用XRD、SEM、TEM、UV-Vis DRS和FTIR等技术对催化剂进行了表征,并通过光催化降解罗丹明B(RhB)评价其光催化性能。实验结果表明:ZrO_(2)与(NH_(4))_(3)PW_(12)O_(40)的配比对催化剂的形貌和光催化性能均有影响,当n(Zr)∶n(W)=1∶5时催化剂的分散性好,光催化活性较高;紫外光照射90 min后,RhB的降解率达到96.69%;重复使用4次后,该催化剂仍保持较高的催化活性。 展开更多
关键词 罗丹明b ZrO_(2) (NH_(4))_(3)PW_(12)O_(40)
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Increased endothelin receptor B and G protein coupled kinase-2 in the mesentery of portal hypertensive rats 被引量:7
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作者 Qing-Hong Du Lin Han +3 位作者 Jun-Jie Jiang Peng-Tao Li Xin-Yue Wang Xu Jia 《World Journal of Gastroenterology》 SCIE CAS 2013年第13期2065-2072,共8页
AIM: To elucidate the mechanisms of mesenteric vasodilation in portal hypertension (PHT), with a focus on endothelin signaling. METHODS: PHT was induced in rats by common bile duct ligation (CBDL). Portal pressure (PP... AIM: To elucidate the mechanisms of mesenteric vasodilation in portal hypertension (PHT), with a focus on endothelin signaling. METHODS: PHT was induced in rats by common bile duct ligation (CBDL). Portal pressure (PP) was measured directly via catheters placed in the portal vein tract. The level of endothelin-1 (ET-1) in the mesenteric circulation was determined by radioimmunoassay, and the expression of the endothelin A receptor (ETAR) and endothelin B receptor (ETBR) was assessed by immunofluorescence and Western blot. Additionally, expression of G protein coupled kinase-2 (GRK2) and β-arrestin 2, which influence endothelin receptor sensitivity, were also studied by Western blot. RESULTS: PP of CBDL rats increased significantly (11.89 ± 1.38 mmHg vs 16.34 ± 1.63 mmHg). ET-1 expression decreased in the mesenteric circulation 2 and 4 wk after CBDL. ET-1 levels in the systemic circulation of CBDL rats were increased at 2 wk and decreased at 4 wk. There was no change in ETAR expression in response to CBDL; however, increased expression of ETBR in the endothelial cells of mesenteric arterioles and capillaries was observed. In sham-operated rats, ETBR was mainly expressed in the CD31+ endothelial cells of the arterioles. With development of PHT, in addition to the endothelial cells, ETBR expression was noticeably detectable in the SMA+ smooth muscle cells of arterioles and in the CD31+ capillaries. Following CBDL, increased expression of GRK2 was also found in mesenteric tissue, though there was no change in the level of β-arrestin 2. CONCLUSION: Decreased levels of ET-1 and increased ETBR expression in the mesenteric circulation following CBDL in rats may underlie mesenteric vasodilation in individuals with PHT. Mechanistically, increased GRK2 expression may lead to desensitization of ETAR, as well as other vasoconstrictors, promoting this vasodilatory effect. 展开更多
关键词 PORTAL HYPERTENSION MESENTERY ENDOTHELIN ENDOTHELIN b receptor G protein COUPLED kinase-2
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弥漫大B细胞淋巴瘤患者血清VEGF、β_2-MG、TRAF6、Bcl-6水平对预后的影响 被引量:1
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作者 何吉琼 李亚琼 +1 位作者 杨丽华 乔爱国 《疑难病杂志》 CAS 2015年第7期708-711,共4页
目的观察血清血管内皮生长因子(VEGF)、β_2微球蛋白(β_2-MG)、TNF受体关联因子6(TRAF6)及B细胞淋巴瘤因子-6(Bcl-6)水平对弥漫大B细胞淋巴瘤(DLBCL)患者的预后影响。方法选取2012年4月—2014年4月收治的51例弥漫大B细胞淋巴瘤初治患者... 目的观察血清血管内皮生长因子(VEGF)、β_2微球蛋白(β_2-MG)、TNF受体关联因子6(TRAF6)及B细胞淋巴瘤因子-6(Bcl-6)水平对弥漫大B细胞淋巴瘤(DLBCL)患者的预后影响。方法选取2012年4月—2014年4月收治的51例弥漫大B细胞淋巴瘤初治患者,记录不同性别、年龄、是否侵犯纵隔、是否合并B组症状、乳酸脱氢酶水平及不同治疗效果的患者例数,分析血清VEGF、β_2-MG、TRAF6及Bcl-6水平对患者预后的影响。结果51例DLBCL患者中,VEGF高水平25例,β_2-MG高水平39例,TRAF6阳性11例,Bcl-6阳性8例。不同性别、不同年龄、是否侵犯纵隔、是否合并B组症状、不同临床分期、乳酸脱氢酶是否升高与血清VEGF、β_2-MG高水平及TRAF6、Bcl-6阳性患者例数之间差异不具有统计学意义(P均>0.05),但β_2-MG、TRAF6、Bcl-6高水平/(阳性)与低水平/(阴性)患者治疗效果差异具有统计学意义(x^2=10.455、7.913、5.004,P=0.001、0.005、0.025)。低危组VEGF、β_2-MG、TRAF6及Bcl-6值分别为(446.31±38.82)ng/ml、(194 3.15±178.28)ng/ml、(1.27±0.60)ng/ml、(13.68±7.32)ng/nml,高危组分别为(482.31±37.71)ng/ml、(2 128.32±243.12)ng/ml、(1.72±0.83)ng/ml、(21.93±6.85)ng/ml,2组相比差异均具有统计学意义(t=3.348、3.040、2.174、4.150,P=0.002、0.004、0.035、0.000)。结论弥漫大B细胞淋巴瘤患者的血清VEGF、β_2-MG、TRAF6、Bcl-6水平有助于判断患者预后,对提高DLBCL患者疗效及制定个体化治疗方案具有重要的指导意义,值得在临床上予以推广。 展开更多
关键词 弥漫大b细胞淋巴瘤 β2 TNF6 b细胞淋巴瘤因子-6
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Serum soluble interleukin-2 receptor levels in patients with chronic hepatitis B virus infection and its relation with antiHBc 被引量:9
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作者 Ping Xiao Qing-Feng Chen Yan-Ling Yang Zhen-Hua Guo Hong Chen 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第3期482-484,共3页
AIM: To investigate the relationship between serum soluble interleukin-2 receptor (sIL-2R) level and anti-HBc in patients with chronic hepatitis B virus (HBV) infection. METHODS: Sera from 100 patients with chro... AIM: To investigate the relationship between serum soluble interleukin-2 receptor (sIL-2R) level and anti-HBc in patients with chronic hepatitis B virus (HBV) infection. METHODS: Sera from 100 patients with chronic HBV infection and 30 healthy controls were included in this study. The patients were divided into group A [HBsAg (+), HBeAg (+) and anti-HBc (+), n = 50] and group B [HBsAg (+), HBeAg (+) and anti-HBc (-), n = 50]. sIL-2R levels were determined using ELISA. HBV DNA and alanine aminotransferase (ALT) were also detected. RESULTS: Serum sIL-2R levels were significantly higher in patients with chronic HBV infection than in healthy controls. Moreover, serum sIL-2R levels were significantly higher in patients with HBsAg (+), HBeAg (+) and antiHBc (+) (976.56±213.51×10^3 U/L) than in patients with HBsAg (+), HBeAg (+) and anti-HBc (-) (393.41±189.54 ×10^3 U/L, P〈 0.01). A significant relationship was found between serum sIL-2R and ALT levels (P〈 0.01) in patients with chronic HBV infection, but there was no correlation between sIL-2R and HBV DNA levels. The anti-HBc status was significantly related to the age of patients (P〈 0.01). CONCLUSION: The high sIL-2R level is related to positive anti-HBc in chronic hepatitis B patients. Positive anti-HBc may be related to T-lymphocyte activation and negative anti-HBc may imply immune tolerance in these patients. 展开更多
关键词 Chronic hepatitis b Hepatitis b virus AntiHbc Soluble interleukin-2 receptor Immune tolerance
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