A method was developed to quickly determine genistin, genistein, kaempferol and rutin in Fructus sophorae by β -CDmodified capillary zone electrophoresis. Based on the investigation of the effects of buffer pH and co...A method was developed to quickly determine genistin, genistein, kaempferol and rutin in Fructus sophorae by β -CDmodified capillary zone electrophoresis. Based on the investigation of the effects of buffer pH and concentration, β -CD concentration,SDS concentration, capillary temperature, the analytical conditions were optimized. Under the optimized conditions, the three analytes were well separated in less than 6 min. A good linear relationship between the peak area and concentration was found in the 2 ～ 400g/mL,2 ～ 300g/mL 2 ～ 200g/mL and 5 ～ 500g/mL concentration range for genistin, genistein, kaempferol and rutin, respectively. The detection limits were 0.8g/mL, 0.6g/mL, 0.6g/mL and 1.4g/mL for genistin, genistein, kaempferol and rutin, respectively.展开更多
文摘A method was developed to quickly determine genistin, genistein, kaempferol and rutin in Fructus sophorae by β -CDmodified capillary zone electrophoresis. Based on the investigation of the effects of buffer pH and concentration, β -CD concentration,SDS concentration, capillary temperature, the analytical conditions were optimized. Under the optimized conditions, the three analytes were well separated in less than 6 min. A good linear relationship between the peak area and concentration was found in the 2 ～ 400g/mL,2 ～ 300g/mL 2 ～ 200g/mL and 5 ～ 500g/mL concentration range for genistin, genistein, kaempferol and rutin, respectively. The detection limits were 0.8g/mL, 0.6g/mL, 0.6g/mL and 1.4g/mL for genistin, genistein, kaempferol and rutin, respectively.
