目的观察雌二醇对去卵巢骨质疏松大鼠骨组织Wnt/β-catenin信号通路的影响,探讨其防治绝经后骨质疏松症的作用机制。方法将80只雌性SD大鼠随机分为对照组、假手术组、去势组、药物组,构建绝经后骨质疏松大鼠模型,检测骨组织骨密度(bone ...目的观察雌二醇对去卵巢骨质疏松大鼠骨组织Wnt/β-catenin信号通路的影响,探讨其防治绝经后骨质疏松症的作用机制。方法将80只雌性SD大鼠随机分为对照组、假手术组、去势组、药物组,构建绝经后骨质疏松大鼠模型,检测骨组织骨密度(bone mineral density,BMD)和生物力学指标,运用qRT-PCR和免疫组化分别检测骨组织Wnt、β-catenin、BMP-2的mRNA和蛋白表达,通过HE染色观察骨组织形态学。结果去势组股骨BMD、生物力学指标较对照组和假手术组均显著降低(P<0.05),药物组股骨BMD、生物力学指标较去势组均显著升高(P<0.05)。去势组骨组织Wnt、β-catenin、BMP-2的mRNA和蛋白表达较对照组和假手术组均显著降低(P<0.05),药物组骨组织Wnt、β-catenin、BMP-2的mRNA和蛋白表达较去势组均显著升高(P<0.05)。去势组骨小梁明显减少,排列稀疏不规则,连接不完整,骨髓腔变大,有大量纤维组织。药物组骨小梁数量减少不明显,粗细均匀稍致密,排列尚规则,连续性完整性较好。结论雌二醇通过Wnt/β-catenin信号通路作用于BMP-2,增加了去卵巢骨质疏松大鼠骨密度,提高了骨生物力学性能,能够改善骨组织结构,发挥了抗骨质疏松作用。展开更多
AIM: To observe the binding activity of beta-2-glycoprotein I (β2GPI) to hepatitis B surface antigen (HBsAg) and the possible roles of β2GPI in hepatitis B virus (HBV) infection.METHODS: The rationale of ELISA metho...AIM: To observe the binding activity of beta-2-glycoprotein I (β2GPI) to hepatitis B surface antigen (HBsAg) and the possible roles of β2GPI in hepatitis B virus (HBV) infection.METHODS: The rationale of ELISA methods and ELISAbased research method and ligand-blotting technique were used to detect the specific interaction of β2GPI with HBsAg.RESULTS: With the increase of rHBsAg, the binding of β2GPI to rHBsAg elevated, and these changes had statistic significance. When we added non- biotinlyated β2GPI, the OD value significantly decreased though they still were positively relevant to rHBsAg, suggesting non- biotinlyated β2GPI competed with biotinlyated β2GPI to saturate the binding sites on rHBsAg. Meanwhile BSA was used as negative control to substitute for rHBsAg coating the plates.The results indicated no interaction between β2GPI and BSA,suggesting the affinity of β2GPI to rHBsAg was specific. The ligand blotling indicated that β2GPI might bind to rHBsAg no matter whether it was under reduced condition or not.CONCLUSION: The binding of β2GPI to HBsAg suggests that β2GPI may be a carrier of HBV and that β2GPI may play important roles in HBV infection.展开更多
文摘目的观察雌二醇对去卵巢骨质疏松大鼠骨组织Wnt/β-catenin信号通路的影响,探讨其防治绝经后骨质疏松症的作用机制。方法将80只雌性SD大鼠随机分为对照组、假手术组、去势组、药物组,构建绝经后骨质疏松大鼠模型,检测骨组织骨密度(bone mineral density,BMD)和生物力学指标,运用qRT-PCR和免疫组化分别检测骨组织Wnt、β-catenin、BMP-2的mRNA和蛋白表达,通过HE染色观察骨组织形态学。结果去势组股骨BMD、生物力学指标较对照组和假手术组均显著降低(P<0.05),药物组股骨BMD、生物力学指标较去势组均显著升高(P<0.05)。去势组骨组织Wnt、β-catenin、BMP-2的mRNA和蛋白表达较对照组和假手术组均显著降低(P<0.05),药物组骨组织Wnt、β-catenin、BMP-2的mRNA和蛋白表达较去势组均显著升高(P<0.05)。去势组骨小梁明显减少,排列稀疏不规则,连接不完整,骨髓腔变大,有大量纤维组织。药物组骨小梁数量减少不明显,粗细均匀稍致密,排列尚规则,连续性完整性较好。结论雌二醇通过Wnt/β-catenin信号通路作用于BMP-2,增加了去卵巢骨质疏松大鼠骨密度,提高了骨生物力学性能,能够改善骨组织结构,发挥了抗骨质疏松作用。
基金the National Natural Science Foundation of China, No.30070338
文摘AIM: To observe the binding activity of beta-2-glycoprotein I (β2GPI) to hepatitis B surface antigen (HBsAg) and the possible roles of β2GPI in hepatitis B virus (HBV) infection.METHODS: The rationale of ELISA methods and ELISAbased research method and ligand-blotting technique were used to detect the specific interaction of β2GPI with HBsAg.RESULTS: With the increase of rHBsAg, the binding of β2GPI to rHBsAg elevated, and these changes had statistic significance. When we added non- biotinlyated β2GPI, the OD value significantly decreased though they still were positively relevant to rHBsAg, suggesting non- biotinlyated β2GPI competed with biotinlyated β2GPI to saturate the binding sites on rHBsAg. Meanwhile BSA was used as negative control to substitute for rHBsAg coating the plates.The results indicated no interaction between β2GPI and BSA,suggesting the affinity of β2GPI to rHBsAg was specific. The ligand blotling indicated that β2GPI might bind to rHBsAg no matter whether it was under reduced condition or not.CONCLUSION: The binding of β2GPI to HBsAg suggests that β2GPI may be a carrier of HBV and that β2GPI may play important roles in HBV infection.
