Objective:To investigate the role of Mirabilis jalapa root extracts in restoration of glucose homeostasis in alloxan-induced hyperglycemic Wistar albino rats.Methods:Experimental hyperglycemic rats were treated daily ...Objective:To investigate the role of Mirabilis jalapa root extracts in restoration of glucose homeostasis in alloxan-induced hyperglycemic Wistar albino rats.Methods:Experimental hyperglycemic rats were treated daily with 200 and 400 mg/kg of Mirabilis jalapa extracts after initial fasting for 6 h.Two-hour postprandial glucose and changes in body weight were monitored during treatment.After 14 d,the rats were sacrificed and blood was collected for biochemical assessment of serum glucose and insulin levels,lipid profile,and oxidative stress markers.Histopathological examinations of harvested pancreas were also carried out.Results:Mirabilis jalapa root extracts at 200 and 400 mg/kg increased the body weight of hyperglycemic rats.Postprandial glucose levels of the extract-treated hyperglycemic groups progressively declined during treatment compared with the untreated hyperglycemic control group(P<0.05).The lipid profile indices of the untreated negative control group were significantly elevated(P<0.05),which were reversed by treatment with Mirabilis jalapa extracts.The remarkable increases in antioxidant enzyme activities and a significant decrease in malondialdehyde levels were observed in the hyperglycemic group treated with Mirabilis jalapa extracts.Mirabilis jalapa extracts also significantly increased serum insulin levels(P<0.05).In addition,histopathological examinations of the pancreas revealed a significant cell population within the islet nests of the extract-treated hyperglycemic groups.Conclusions:Mirabilis jalapa extract can restore glucose homeostasis and show hypoglycemic and hypolipidemic effects in hyperglycemic rats.Further studies are needed to verify the active components of the plant and the underlying mechanism of action in the future.展开更多
目的探讨运动与中药对糖尿病大鼠胰岛β细胞功能指数(isletβ-cell function index,HOMA-%β)、血糖及氧化应激状态的影响。方法 Wistar雄性大鼠60只,用小剂量链脲佐菌素加高脂饲料的方法建立糖尿病大鼠模型,观察运动、中药丹蛭降糖胶...目的探讨运动与中药对糖尿病大鼠胰岛β细胞功能指数(isletβ-cell function index,HOMA-%β)、血糖及氧化应激状态的影响。方法 Wistar雄性大鼠60只,用小剂量链脲佐菌素加高脂饲料的方法建立糖尿病大鼠模型,观察运动、中药丹蛭降糖胶囊及两者联用对糖尿病大鼠空腹血糖(FBG)、空腹血清胰岛素(FINS)、甘油三酯(TG)、总胆固醇(TC)、胰腺匀浆中的谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、丙二醛(MDA)的影响,并计算HOMA-%β,用多因素逐步回归分析探讨影响HOMA-%β的主要因素。结果与模型组比较,单纯运动或中药干预后,大鼠FBG、TG、TC、胰腺MDA均明显降低(P<0.05,P<0.01),HOMA-%β明显升高(P<0.01),运动组胰腺GSH-Px活性明显升高(P<0.01);运动与中药联用对FBG、TG、HOMA-%β、胰腺SOD、GSH-Px改变有协同作用(P<0.05)。FBG与胰腺SOD、GSH-Px呈显著负相关且有线性回归关系,HOMA-%β与FBG、TG、TC、胰腺MDA水平负相关,与胰腺SOD、GSH-Px水平正相关,且与FBG有线性回归关系。结论运动及丹蛭降糖胶囊可发挥协同作用,能明显改善糖脂代谢、提高胰腺抗氧化水平,从而减轻胰岛β细胞损伤。展开更多
基金Institutional Based Research,(TeTFund/DR&D/CE/UNIV/SOKOTO/RP/VOL 1)Usmanu Danfodiyo University,Sokoto.
文摘Objective:To investigate the role of Mirabilis jalapa root extracts in restoration of glucose homeostasis in alloxan-induced hyperglycemic Wistar albino rats.Methods:Experimental hyperglycemic rats were treated daily with 200 and 400 mg/kg of Mirabilis jalapa extracts after initial fasting for 6 h.Two-hour postprandial glucose and changes in body weight were monitored during treatment.After 14 d,the rats were sacrificed and blood was collected for biochemical assessment of serum glucose and insulin levels,lipid profile,and oxidative stress markers.Histopathological examinations of harvested pancreas were also carried out.Results:Mirabilis jalapa root extracts at 200 and 400 mg/kg increased the body weight of hyperglycemic rats.Postprandial glucose levels of the extract-treated hyperglycemic groups progressively declined during treatment compared with the untreated hyperglycemic control group(P<0.05).The lipid profile indices of the untreated negative control group were significantly elevated(P<0.05),which were reversed by treatment with Mirabilis jalapa extracts.The remarkable increases in antioxidant enzyme activities and a significant decrease in malondialdehyde levels were observed in the hyperglycemic group treated with Mirabilis jalapa extracts.Mirabilis jalapa extracts also significantly increased serum insulin levels(P<0.05).In addition,histopathological examinations of the pancreas revealed a significant cell population within the islet nests of the extract-treated hyperglycemic groups.Conclusions:Mirabilis jalapa extract can restore glucose homeostasis and show hypoglycemic and hypolipidemic effects in hyperglycemic rats.Further studies are needed to verify the active components of the plant and the underlying mechanism of action in the future.
文摘目的探讨运动与中药对糖尿病大鼠胰岛β细胞功能指数(isletβ-cell function index,HOMA-%β)、血糖及氧化应激状态的影响。方法 Wistar雄性大鼠60只,用小剂量链脲佐菌素加高脂饲料的方法建立糖尿病大鼠模型,观察运动、中药丹蛭降糖胶囊及两者联用对糖尿病大鼠空腹血糖(FBG)、空腹血清胰岛素(FINS)、甘油三酯(TG)、总胆固醇(TC)、胰腺匀浆中的谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、丙二醛(MDA)的影响,并计算HOMA-%β,用多因素逐步回归分析探讨影响HOMA-%β的主要因素。结果与模型组比较,单纯运动或中药干预后,大鼠FBG、TG、TC、胰腺MDA均明显降低(P<0.05,P<0.01),HOMA-%β明显升高(P<0.01),运动组胰腺GSH-Px活性明显升高(P<0.01);运动与中药联用对FBG、TG、HOMA-%β、胰腺SOD、GSH-Px改变有协同作用(P<0.05)。FBG与胰腺SOD、GSH-Px呈显著负相关且有线性回归关系,HOMA-%β与FBG、TG、TC、胰腺MDA水平负相关,与胰腺SOD、GSH-Px水平正相关,且与FBG有线性回归关系。结论运动及丹蛭降糖胶囊可发挥协同作用,能明显改善糖脂代谢、提高胰腺抗氧化水平,从而减轻胰岛β细胞损伤。
