This study presents an unexpected finding that the cis isomer of β-thio-Asp exhibits higher ligation ac-tivity than the trans isomer.This discovery sheds light on the intricate nature of native chemical ligation and ...This study presents an unexpected finding that the cis isomer of β-thio-Asp exhibits higher ligation ac-tivity than the trans isomer.This discovery sheds light on the intricate nature of native chemical ligation and highlights the importance of factors beyond the steric effects of the side chain in modulating ligation activity.展开更多
基金the CAMS Innovation Fund for Medical Sciences (CIFMS,No.2021-I2M-1-026)the National Key R & D Program of China (No.2018YFE0111400)+5 种基金the NIH Research Project Grant Program (No.R01 EB025892)the National Natural Science Foundation of China (the Training Program of the Major Research Plan,No.91853120)the National Major Scientific and Technological Special Project of China (Nos.2018ZX09711001-005 and 2018ZX09711001-013)the State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medicathe Biomedical High Performance Computing Platform,Chinese Academy of Medical Sciences,the Chinese Academy of Medical SciencesPeking Union Medical College for funding and support
文摘This study presents an unexpected finding that the cis isomer of β-thio-Asp exhibits higher ligation ac-tivity than the trans isomer.This discovery sheds light on the intricate nature of native chemical ligation and highlights the importance of factors beyond the steric effects of the side chain in modulating ligation activity.
