Induction of non-specific toxicities by doxorubicin(DOX) has restricted conventional DOXbased chemotherapy. p H-responsive dextrin nanogels(DNGs) have been fabricated in order to incorporate and deliver DOX to specifi...Induction of non-specific toxicities by doxorubicin(DOX) has restricted conventional DOXbased chemotherapy. p H-responsive dextrin nanogels(DNGs) have been fabricated in order to incorporate and deliver DOX to specific(targeted) sites. However, adequate stability studies of DOX-loaded DNGs are required for selection of storage conditions. The aim of this study was therefore to evaluate the accelerated(25 °C/60% RH) and long-term(5 °C) stability of DNGs prepared with formaldehyde(FDNGs) and glyoxal(GDNGs) as cross-linker by determining the change in their physicochemical properties. The mean diameter decreased with time during long-term storage. The drug content between freshly prepared(initial day) and after storage at 5 °C for 180 days of DOX-loaded FDNGs and DOX-loaded GDNGs was not significantly different(p > 0.05), but decreased after storage under the accelerated condition. The release of DOX from all DNGs was pH-dependent. However, DNGs kept under the accelerated condition showed higher amount of DOX release than those stored at 5 °C and the freshly prepared ones. The results indicate that the stability of DNGs could be improved by their storage at 5 °C.展开更多
As part of a systematic research on the interaction mechanism between polysaccharides and mineral surfaces, ive extend the studies on dextrin adsorption to real minerals such as oxides and salt-type minerals, based on...As part of a systematic research on the interaction mechanism between polysaccharides and mineral surfaces, ive extend the studies on dextrin adsorption to real minerals such as oxides and salt-type minerals, based on previous studies of model systems {surface-modified quartz ). The minerals tested include hematite, rutile, fluorite, calcite, apatite, etc. It is observed that dextrin adsorption on these minerals is strongly pH-depen-dent, with adsorption maxima appearing at pH values around the isoelec-tric points of the minerals, indicating interactions zvith surface metal hy-droxide species. X-ray photoelectron spectroscopy (XPS) measurements re-veal electronic binding energy shifts or Auger parameter changes of miner-al surface elements after dextrin adsorption , pointing to a chemical mecha-nism of interaction. Furthermore, it is observed that the reaction of miner-als with moderate amounts of fatty acids prior to dextrin addition results in an increase in dextrin adsorption, whereas excessive fatty acids do the opposite. Combined with our previous studies, it is concluded from the ex-perimental observations that although surface hydrophobic species alone do not have any effect on dextrin adsorption, they synergistically enhance the adsorption when present together with surface metallic sites.展开更多
Curcumin is a natural polyphenol with anti-oxidative, anti-inflammatory and anti-cancer properties. Its therapeutic potential is substantially hindered by the rather low water solubility and bioavailability, hence the...Curcumin is a natural polyphenol with anti-oxidative, anti-inflammatory and anti-cancer properties. Its therapeutic potential is substantially hindered by the rather low water solubility and bioavailability, hence the need for suitable carriers. In this study, we show that self-assembled nanogels obtained from hydrophobically modified dextrin are effective curcumin nanocarriers. The stability and loading efficiency of curcumin-loaded nanogel depends on the nanogel/curcumin ratio. Higher stability of the formulation is achieved in water than in PBS buffer, as evaluated by dynamic light scattering and fluorescence measurements. The in vitro release profile, using sink conditions, indicates that dextrin nanogel may perform as a suitable carrier for the controlled release of curcumin. Biological activity of curcumin-loaded nanogel in HeLa cell cultures was assessed using the MTS assay.展开更多
Objective:Adhesion barriers have not traditionally been used during laparoscopic cholecystectomy(lapchole),and so there are no data confirming the safety of the dextrin hydrogel adhesion barrier(AdSpray®)for the ...Objective:Adhesion barriers have not traditionally been used during laparoscopic cholecystectomy(lapchole),and so there are no data confirming the safety of the dextrin hydrogel adhesion barrier(AdSpray®)for the use during lap-chole.The aim of this prospective study is to investigate the safety of AdSpray®for elective lap-chole.Methods:Between July 2019 and March 2021,we conducted a prospective study at Saiseikai Kumamoto Hospital to assess the safety of applying AdSpray®around the hepatoduodenal ligament,liver surface,and port area after extraction of the gallbladder during elective lap-chole.We assessed patient morbidity,mortality,and laboratory data on postoperative day 1 and at the outpatient follow-up visit.Results:A total of 100 patients(43 men,57 women)received AdSpray®application during elective lapchole at our institution.The mean patient age was 56.5±14.8 y,and the mean body mass index was 25.1±4.8 kg/m2.Preoperative gallbladder drainage was performed in 3(3.0%)patients as conservative treatment for acute cholecystitis.The mean operative time was 91.7±35.1 min,and the median blood loss was 5 mL(range,5e120 mL).Intraoperative gallbladder perforation was observed in 4.0%of patients(n=4).There was no intraoperative bile duct injury.One(1.0%)patient had a postoperative subhepatic fluid collection without bacterial infection.The mean white blood cell count and C-reactive protein level was significantly elevated on postoperative day 1 but returned to preoperative levels by the time of the follow-up visit.All patients were successfully discharged after surgery,and the median postoperative hospital stay was 2 d(range,2e9 d).Conclusion:Applying AdSpray®during elective lap-chole is safe,with an acceptable rate of postoperative complications.展开更多
Starch ofavocado seeds can be used as an alternative source of starch because it contains 80.1% starch. Natural starch can be made into a modified starch, for example dextrin. Dextrin can be made by using acid or enzy...Starch ofavocado seeds can be used as an alternative source of starch because it contains 80.1% starch. Natural starch can be made into a modified starch, for example dextrin. Dextrin can be made by using acid or enzyme hydrolysis. The purposes of this research were determine the concentration of hydrochloric acid （HCl）, the temperature and heating timein the optimum process of hydrolysis of starch into dextrin and to determine the quality of the resulting product dextrin terms of Indonesian National Standard 01-2593-1992. The methode was used in this research is hydrolisis with hydrochloric acid （HCl）. The results processing into starch avocado seed yield is 23.15%. HCl concentration, temperature and heating time significantly affect the value of dextrose equivalent （DE）, viscosity and part soluble in cold water. There is interaction between HCl concentration, temperature and heating time on the value of DE, viscosity and part soluble in cold water. Optimum conditions of process was obtained at a concentration of 0.15 N HCl, 30 minutes heating time and heating temperature of 90 ℃. Dextrose equivalent （DE） value is 19.61%. The value of solubility in cold water is 90.19%. Viscosity value is 1.61 ° E. Dextrin is not accordance with the standards of quality parameters Indonesian National Standard 01-2593-1992. Dextrin produced should be applied to non-food industry.展开更多
文摘Induction of non-specific toxicities by doxorubicin(DOX) has restricted conventional DOXbased chemotherapy. p H-responsive dextrin nanogels(DNGs) have been fabricated in order to incorporate and deliver DOX to specific(targeted) sites. However, adequate stability studies of DOX-loaded DNGs are required for selection of storage conditions. The aim of this study was therefore to evaluate the accelerated(25 °C/60% RH) and long-term(5 °C) stability of DNGs prepared with formaldehyde(FDNGs) and glyoxal(GDNGs) as cross-linker by determining the change in their physicochemical properties. The mean diameter decreased with time during long-term storage. The drug content between freshly prepared(initial day) and after storage at 5 °C for 180 days of DOX-loaded FDNGs and DOX-loaded GDNGs was not significantly different(p > 0.05), but decreased after storage under the accelerated condition. The release of DOX from all DNGs was pH-dependent. However, DNGs kept under the accelerated condition showed higher amount of DOX release than those stored at 5 °C and the freshly prepared ones. The results indicate that the stability of DNGs could be improved by their storage at 5 °C.
文摘As part of a systematic research on the interaction mechanism between polysaccharides and mineral surfaces, ive extend the studies on dextrin adsorption to real minerals such as oxides and salt-type minerals, based on previous studies of model systems {surface-modified quartz ). The minerals tested include hematite, rutile, fluorite, calcite, apatite, etc. It is observed that dextrin adsorption on these minerals is strongly pH-depen-dent, with adsorption maxima appearing at pH values around the isoelec-tric points of the minerals, indicating interactions zvith surface metal hy-droxide species. X-ray photoelectron spectroscopy (XPS) measurements re-veal electronic binding energy shifts or Auger parameter changes of miner-al surface elements after dextrin adsorption , pointing to a chemical mecha-nism of interaction. Furthermore, it is observed that the reaction of miner-als with moderate amounts of fatty acids prior to dextrin addition results in an increase in dextrin adsorption, whereas excessive fatty acids do the opposite. Combined with our previous studies, it is concluded from the ex-perimental observations that although surface hydrophobic species alone do not have any effect on dextrin adsorption, they synergistically enhance the adsorption when present together with surface metallic sites.
基金This research was supported by Fundacao para a Ciencia e a Tecnologia under grant SFRH/BD/22242/2005.
文摘Curcumin is a natural polyphenol with anti-oxidative, anti-inflammatory and anti-cancer properties. Its therapeutic potential is substantially hindered by the rather low water solubility and bioavailability, hence the need for suitable carriers. In this study, we show that self-assembled nanogels obtained from hydrophobically modified dextrin are effective curcumin nanocarriers. The stability and loading efficiency of curcumin-loaded nanogel depends on the nanogel/curcumin ratio. Higher stability of the formulation is achieved in water than in PBS buffer, as evaluated by dynamic light scattering and fluorescence measurements. The in vitro release profile, using sink conditions, indicates that dextrin nanogel may perform as a suitable carrier for the controlled release of curcumin. Biological activity of curcumin-loaded nanogel in HeLa cell cultures was assessed using the MTS assay.
文摘Objective:Adhesion barriers have not traditionally been used during laparoscopic cholecystectomy(lapchole),and so there are no data confirming the safety of the dextrin hydrogel adhesion barrier(AdSpray®)for the use during lap-chole.The aim of this prospective study is to investigate the safety of AdSpray®for elective lap-chole.Methods:Between July 2019 and March 2021,we conducted a prospective study at Saiseikai Kumamoto Hospital to assess the safety of applying AdSpray®around the hepatoduodenal ligament,liver surface,and port area after extraction of the gallbladder during elective lap-chole.We assessed patient morbidity,mortality,and laboratory data on postoperative day 1 and at the outpatient follow-up visit.Results:A total of 100 patients(43 men,57 women)received AdSpray®application during elective lapchole at our institution.The mean patient age was 56.5±14.8 y,and the mean body mass index was 25.1±4.8 kg/m2.Preoperative gallbladder drainage was performed in 3(3.0%)patients as conservative treatment for acute cholecystitis.The mean operative time was 91.7±35.1 min,and the median blood loss was 5 mL(range,5e120 mL).Intraoperative gallbladder perforation was observed in 4.0%of patients(n=4).There was no intraoperative bile duct injury.One(1.0%)patient had a postoperative subhepatic fluid collection without bacterial infection.The mean white blood cell count and C-reactive protein level was significantly elevated on postoperative day 1 but returned to preoperative levels by the time of the follow-up visit.All patients were successfully discharged after surgery,and the median postoperative hospital stay was 2 d(range,2e9 d).Conclusion:Applying AdSpray®during elective lap-chole is safe,with an acceptable rate of postoperative complications.
文摘Starch ofavocado seeds can be used as an alternative source of starch because it contains 80.1% starch. Natural starch can be made into a modified starch, for example dextrin. Dextrin can be made by using acid or enzyme hydrolysis. The purposes of this research were determine the concentration of hydrochloric acid （HCl）, the temperature and heating timein the optimum process of hydrolysis of starch into dextrin and to determine the quality of the resulting product dextrin terms of Indonesian National Standard 01-2593-1992. The methode was used in this research is hydrolisis with hydrochloric acid （HCl）. The results processing into starch avocado seed yield is 23.15%. HCl concentration, temperature and heating time significantly affect the value of dextrose equivalent （DE）, viscosity and part soluble in cold water. There is interaction between HCl concentration, temperature and heating time on the value of DE, viscosity and part soluble in cold water. Optimum conditions of process was obtained at a concentration of 0.15 N HCl, 30 minutes heating time and heating temperature of 90 ℃. Dextrose equivalent （DE） value is 19.61%. The value of solubility in cold water is 90.19%. Viscosity value is 1.61 ° E. Dextrin is not accordance with the standards of quality parameters Indonesian National Standard 01-2593-1992. Dextrin produced should be applied to non-food industry.
