The clinical study of ultrasound-guided intra-hysteromyoma injection of ethyl alcohol absolute combined with HIFU in the treatment of uterine fibroids

Objective The aim of this study was to investigate the energy deposition in the target area of high intensity focused ultrasound （HIFU） with ultrasound-guided intra-hysteromyoma injection of ethyl alcohol absolute Methods Eighty patients with hysteromyomas were randomly divided into two groups： group HIFU （group H） and group HIFU combined with ullrasound-guided inlra-hysteromyoma injectionn of ethyl alcohol absolute （group E ＋ H）. Patients in group E ＋ H received an ullrasound-guided injection of absolute ethyl alcohol 1 h before HIFU treatment. The irradiation time, irradiation dose, energy efficiency factor （radiation energy required for ablation of a myoma per unit of volume）, grey variation, pain score, and adverse reactions were compared between the two groups. An independent sample t-test was used with a two- tailed P-value of 〈 0.05. Results The irradiation time, irradiation dose, and energy efficiency factor were significantly lower in group E ＋ H than those in group H （P 〈 0.05）. The hysteromyomas of patients in group E＋H appeared as dominantly hyperechoic masses, and those of patients in group H appeared as hyperechoic tissue （non- mass）. The incidence of adverse reactions and pain score were higher in group H than those in group E ＋ H （P 〈 0.05）. Conclusion Ultrasound-guided inlra-hysteromyoma injection of ethyl alcohol absolute can enhance the energy deposition of HIFU in the uterine fibroids, improve the treatment efficiency, shorten the treatment time, reduce the treatment dose, and reduce the pain and complications for the patients.

Inhibition of EGFR Suppresses Ethyl Alcohol and Tobacco Cell Effects on Growth of Human Oral Keratinocytes and Human Papillomavirus 16 Entry as a Function of Furin

Background: Reported are increased risks for malignant transformation in human oral keratinocytes (HOK) from ethyl alcohol (ETOH), tobacco products or human papilloma virus oncogenic subtype 16 (HPV 16) infections. We examined various HOK cell responses to these factors to show inhibitors of epidermal growth factor receptor (EGFR) also inhibits furin;proprotein convertase (FC) and HPV 16 entry in HOK. Methods: Immortalized HOK by HPV 16 (HPV 16B) or human telomerase (hTERT);primary foreskin keratinocytes (NHFK), primary HOK, buccal keratinocytes (NHBK) and oral SCC-25 were treated with dibenz[a,l]pyrene (DBP), anthraquinone;nitrosamine (NNAL) or ethyl alcohol (ETOH) and acetaldehyde (AA). ETOH was tested for synthesis of malondialdehyde (MDA) and alcohol dehydrogenase expression (ADH). ETOH, and PAH were evaluated by Western immunoblot for oncogene changes, and phosphorylated EGFR expression. Inhibition of EGFR by WZ4002 and Erlotinib and/or carcinogens effect on HPV 16 entry were studied. A green fluorescent pseudovirus (PsV);chloromethylketone (CMK) an inhibitor of furin activity and Western immunoblot of furin cell distribution further characterized HPV 16 entry. Results: ETOH (10 μM) increased expression of phosphorylated EGFR and HPV 16 entry through furin activity, and membrane, nuclear and cytoskeletal accumulations. CMK suppressed HPV 16 entry and blockage of ADH while aldehyde dehydrogenase (ALDH) enhanced HPV 16 entry. Similarly PAH, DBP (4-8 nM), anthraquinone (98 nM) and NNAL (6.9 μM) enhanced HPV 16 entry through furin activity and membrane, nuclear and cytoskeletal accumulations. Furthermore, WZ4002 and Erlotinib suppressed expressions of phosphorylated EGFR, FC activity, and HPV 16 entry. ETOH and DBP treatments also enhanced expressions of protease activated receptor-1 (PAR-1), and p21waf1 while depressed p16 and p27KIP1 expressions in HOK/HPV 16B cells. Conclusion: EGFR inhibitors are candidates for suppression of alcohol and tobacco effects on EGFR phosphorylated expression;keratinocyte growth, and HPV 16 entry and prevention treatment for HPV related diseases.

Isobaric Vapor–Liquid Equilibrium for tert-Butyl Alcohol + Water + Propane-1,3-Diol + 1-Ethyl-3-Methylimidazolium Chloride at 101.3 kPa

In this study, we used a mixture of organic liquid propane-1,3-diol and ionic liquid 1-ethyl-3-methylimidazolium chloride([emim]Cl) as the entrainer to separate tert-butyl alcohol(TBA) + water. We measured the isobaric vapor–liquid equilibrium(VLE) for the quaternary system TBA + water + propane-1,3-diol + [emim]Cl at 101.3 kPa, and found the VLE data to be well correlated with the nonrandom two-liquid model. These results show that the mixed solvent of propane-1,3-diol + [emim]Cl can increase the relative volatility of TBA to water and break the azeotropic point. We found no notable synergetic effect between them, and observed that the liquid mixed solvent of propane-1,3-diol and [emim]Cl had lower viscosity than [emim]Cl, which makes it a promising entrainer for separating the TBA + water azeotrope in industrial applications.

Dosimeter Film Based on Ethyl Violet-Bromophenol Blue Dyed Poly(Vinyl Alcohol)

Dyed poly(vinyl alcohol) (PVA) films, prepared by a simple technique of casting aqueous solutions of PVA containing a mixture of Ethyl violet and bromophenol blue (EV-BPB) on a horizontal glass plate, are useful as routine high-dose dosimeter in the 1 - 30 kGy range. The color of films changes from violet to yellow when exposed to gamma radiation. The response of this dosimeter depends up on the concentration of chloral hydrate (CH) in the polymer material. The radiation chemical yield (G-value) of PVA dyed film was calculated and found to increase by increasing concentration of chloral hydrate. Post-irradiation storage on the response of the films are discussed. The overall combined uncertainty (at 2σ) associated with measurement of response (ΔA mm-1) at 600 nm for dose range 1 - 15 kGy is 5.6%.

由苯甲醇合成苯甲酸乙酯综合制备实验的课程思政设计

将苯甲酸合成与苯甲酸乙酯制备两个经典实验改造为综合实验,并以改进合成过程实现绿色、高效转化为案例,引导学生通过查阅文献,综合分析合成过程,指出原合成方法存在的问题和不足,提出改进的建议,在此过程中,引导学生将法律法规、实验规范、学科伦理、绿色理念、环保和可持续发展理念等融入实验的设计和实施全过程,使学生在受到基本操作训练的同时得到素质的全面提升。

“跨界药王”评选大赛

借用拟人化和第一人称的手法,以比赛中的主持人介绍和自我介绍的形式对10种不同的曾经或仍然具有其他用途的药物进行简要介绍。

活性炭过滤对黄酒品质的影响

黄酒生产贮存过程中酒体经常会产生异杂味和少许沉淀等。活性炭因其强大的吸附能力,广泛应用于酒类的后期处理。该实验将黄酒经过酒类活性炭过滤,不仅去除了异杂味,且对于黄酒中的一些非喜好代谢物质产生了吸附作用,例如杂醇、生物胺、嘌呤、氨基甲酸乙酯等物质。结果表明,活性炭添加量越大,对以上物质的吸附量就越大,但对原酒样的色度和风味损失也越大。最佳吸附条件为:活性炭添加量10 g/L,吸附温度35℃、吸附时间9 h。此时黄酒感官评价得分85分(初始82分),总酸降低27.96%,且氨基酸态氮损失不大。杂醇降低14.22%,生物胺降低31.10%,嘌呤降低27.78%,氨基甲酸乙酯降低30.77%,酯类风味物质保持较好。该研究可对黄酒工业化生产过程中品质的控制提供一种便捷有效的方法。

血浆脂肪酸乙酯与酒精性急性胰腺炎炎症和疾病严重程度的相关性研究

目的分析血浆脂肪酸乙酯(FAEE)与酒精性急性胰腺炎(AAP)炎症和疾病严重程度的相关性。方法回顾性分析2017年1月至2022年1月该院收治的227例AAP患者及213例非酒精性急性胰腺炎(AP)组患者数据。根据Bathazar计算机断层扫描严重指数将AAP患者的疾病严重程度分为轻度、中度、重度。通过气相色谱分析患者血浆FAEE和游离脂肪酸(NEFA)水平。结果AAP组患者血浆FAEE(P<0.001)和NEFA(P=0.019)水平相较于非酒精性AP组更高。然而,经受试者工作特征(ROC)曲线分析,血浆FAEE对鉴别诊断AAP与非酒精性AP有良好效能,曲线下面积(AUC)为0.926(95%CI:0.901~0.951)。中度[158.77(127.01~230.55)nmol/L]和重度[274.49(208.32~309.31)nmol/L]AAP患者血浆FAEE水平高于轻度患者[126.15(84.37~166.52)nmol/L],而且重度患者较中度患者更高(H=64.069,P<0.001)。血浆FAEE水平>146.61 nmol/L为重度AAP发生的危险因素(P<0.05),且其对重度AAP有较好的预测价值,AUC为0.786(95%CI:0.705~0.867)。Spearman秩相关分析结果显示,AAP患者血浆FAEE水平与白细胞介素(IL)-1β、IL-6及NEFA水平呈正相关(rS=0.298、0.475、0.302,均P<0.001)。结论血浆FAEE有可能成为AAP鉴别诊断和预测疾病严重程度的潜在生物标志物。

传统黄酒的风味成分及品质控制研究进展

黄酒作为中国传统酿造酒,营养丰富,口感香醇。不同的黄酒具有不同的风味特征,通过对黄酒风味物质的分析能够探究不同黄酒的品质特征。醇类、酯类、酚类、酸类以及醛类等挥发性风味物质是决定黄酒香气特征与品质的重要因素。然而,黄酒中过量的高级醇、氨基甲酸乙酯、甲醇和生物胺等成分对黄酒品质存在潜在的不利影响,并且在去除的过程中也会一定程度上影响黄酒的风味物质。该文介绍了黄酒中的各种特征风味物质,阐述了黄酒品质的主要控制方法,同时总结了影响黄酒品质的主要因素控制及其对风味的影响,并对黄酒的现存问题和发展前景进行了阐述和展望。

Mucosal permeability to lipopolysaccharides in the colon in chronic alcoholic rats

AIMS To evaluate the effects of chronic alcohol abuse on the mucosal permeability to lipopolysaccharide in the colon in rats. METHODS Escherichia coil lipopolysaccharide (LPS,20 μg/ml) was injected into the colon of chronic alcoholic rats (n=10) and the rats were supplied with Lieber diets every other day for 6 weeks. Before LPS injection and 5,10,20,30 minutes after injection, blood samples from the portal vein were obtained and contents of LPS in the blood were measured. The dis- tribution of LPS in the colon tissues was observed with a confocal laser scanning microscope by immunofluo- rescent technique using a monoclonal antibody specific to the lipid A region of LPS. Normal rats were used as controls (n=6). RESULTS Before LPS injection in the colon,LPS levels in the blood of portal vein of chronic alcoholic rats were significantly higher than those of normal con- trols (3.56±0.67 pg/ml,vs 2.45±0.15 pg/ml,P <0.01). At 5,10,20,30 minutes after injection of LPS,LPS contents were significantly higher than those before LPS injection (173.56±23.45 pg/ml,154.78 ±20.57 pg/ml,43.89±8.67 pg/ml,45.38± 7.89 pg/mls vs 3.56±0.67 pg/ml,P<0.01 respectively). Most mucosal cells showed strong posi- tive reactions to LPS in the rats of chronic alcohol abuse,but no significant changes of LPS contents in blood from the portal vein and fluorescent reactions to LPS in mucosal cells of normal rats were found after LPS injection. CONCLUSIONS Chronic alcohol abuse resulted in a significant increase of permeability to LPS in colon mu- cosal cells in rats.

乙醇-水体系的间歇萃取精馏研究

使用加盐间歇萃取精馏的方法制取无水乙醇,探究在以丙三醇为萃取剂的情况下,回流比、无水氯化钙质量和原料乙醇质量分数对乙醇水溶液分离效果的影响。研究结果表明,加入无水氯化钙的质量较少时,会降低产品乙醇的质量分数。

不同大孔吸附树脂和酒用活性炭对浓香型白酒中醇酯的影响

目前浓香型白酒普遍存在己酸乙酯不足、乳酸乙酯或乙酸乙酯偏高现象,严重影响白酒品质。该研究比较五种大孔吸附树脂(极性DA-201、中极性DM-301、弱极性AB-8、非极性D-101、非极性D4006)和三种碘值与比表面积相同的酒用活性炭(0.5-1 mm颗粒1#、1-2 mm颗粒2#、4-8 mm片状颗粒3#)在不同时间(24 h、48 h、72 h)下对白酒中醇酯的吸附作用。结果表明,两类材料均对酒体中己酸乙酯、丁酸乙酯、乙缩醛和乙酸乙酯表现出吸附能力;树脂类对醇类物质的含量吸附前后基本无变化;活性炭对醇类物质的吸附含量有微量增长。五种大孔吸附树脂中,弱极性树脂AB-8整体吸附能力更强,但极性树脂DA-201乙酸乙酯吸附价值更高,72 h时,DA-201的己酸乙酯吸附作用(吸附率24.34%、吸附量3.33 mg/g)约为AB-8的50%,二者乙酸乙酯吸附能力相当(吸附率36.39%、吸附量13.60 mg/g)。三种酒用活性炭中,片状颗粒的整体吸附效果最佳,随吸附时间的延长而增加,72 h时,片状颗粒3#己酸乙酯吸附率16.60%、吸附量170.10 mg/g,乙酸乙酯吸附率36.65%、吸附量1022.88 mg/g,优于大孔吸附树脂,表明定向制备活性炭是有效转化白酒醇酯异常指标为正常指标的一条途径。

水工混凝土浸渍型防护涂料抗冰拔性能试验研究

高寒地区的水利大坝等水工混凝土建筑物在结冰期间易出现建筑外层与覆冰层发生抗冰拔破坏的现象,降低了建筑物的耐久性和服役寿命。通过防护涂料中端羟基硅油(PDMS)掺杂不同含量正硅酸乙酯(TEOS)后对水泥基抗冰拔防护性能影响的试验,以及不同浓度梯度的异丙醇(IPA)掺量对涂料渗透性和耐腐蚀性影响的试验,研发出一种以TEOS、PDMS、IPA和乙醇(EtOH)为主要成分,用于高寒地区水利大坝迎水面的浸渍型防护涂料,并与其他品种涂料进行抗冰拔能力对比试验。结果表明:涂料的抗冰拔性能随TEOS用量的增加先增高后降低,TEOS在涂料中质量分数为21%时效果最好;添加18%(质量分数)的IPA可有效减少涂料在使用过程中被污染而导致使用寿命缩短的现象;所研发的防护涂料在与同类型涂料进行抗冰拔与水接触角对比试验中取得了较为良好的效果,接触角为121.5°;提出的检测水泥基与冰层之间冰拔力的试验方法,可以作为检验水泥基涂料抗冰拔能力的一种途径。

Alcoholic pancreatitis:New insights into the pathogenesisand treatment

Acute pancreatitis is a necro-inflammatory disease of the exocrine pancreas that is characterized by inappropriate activation of zymogens, infiltration of the pancreas by inflammatory cells, and destruction of the pancreatic exocrine cells. Acute pancreatitis can progress to a severe life-threatening disease. Currently there is no pharmacotherapy to prevent or treat acute pancreatitis. One of the more common factors associated with acute pancreatitis is alcohol abuse. Although commonly associated with pancreatitis alcohol alone is unable to cause pancreatitis. Instead, it appears that alcohol and its metabolic by-products predispose the pancreas to damage from agents that normally do not cause pancreatitis, or to more severe disease from agents that normally cause mild pancreatic damage. Over the last 10 to 20 years, a tremendous amount of work has defined a number of alcohol-mediated biochemical changes in pancreatic cells. Among these changes are: Sustained levels of intracellular calcium, activation of the mitochondrial permeability transition pore, endoplasmic reticulum stress, impairment in autophagy, alteration in the activity of transcriptional activators, and colocalization of lysosomal and pancreatic digestive enzymes. Elucidation of these changes has led to a deeper understanding of the mechanisms by which ethanol predisposes acinar cells to damage. This greater understanding has revealed a number of promising targets for therapeutic intervention. It is hoped that further investigation of these targets will lead to the development of pharmacotherapy that is effective in treating and preventing the progression of acute pancreatitis.

Liquid Chromatography-Tandem Mass Spectrometry Assay to Detect Ethyl Glucuronide in Human Fingernail: Comparison to Hair and Gender Differences

Over the past decade, the use of hair specimens for the long-term detection of the alcohol biomarker ethyl glucuronide has been increasing in popularity and usage. We evaluated the usefulness of fingernail clippings as a suitable alterna-tive to hair for ethyl glucuronide detection. A liquid chromatography-tandem mass spectrometry method for the detection of ethyl glucuronide in fingernail clippings was fully validated and used to analyze the hair and/or fingernail specimens of 606 college-aged study participants. The limit of detection was 2 pg/mg, the limit of quantitation was 8 pg/mg and the method was linear from 8 to 2000 pg/mg. Intra- and inter-assay imprecision studies at three different concentrations (20, 40, 200 pg/mg) were all within 7.8% and all intra- and inter-assay bias studies at these levels were within 115.1% of target concentration. Ethyl glucuronide levels in fingernail (mean = 29.1 ± 55.6 pg/mg) were higher than ethyl glucuronide levels in hair (mean = 9.48 ± 22.3 pg/mg) and a correlation of the matched pairs was observed (r = 0.552, P < 0.01, n = 529). Evaluating each gender separately revealed that the correlation of male fingernail to male hair was large and significant (r = 0.782, P < 0.01, n = 195) while female hair to female fingernail was small yet sig-nificant (r = 0.249, P < 0.01, n = 334). The study results demonstrated that fingernail may be a suitable alternative to hair for ethyl glucuronide detection and may be the preferred sample type due to the lack of a gender bias.

Biomarkers for detection of alcohol consumption in liver transplantation

Alcoholic liver disease is an established, yet controversial, indication for liver transplantation. Although an abstinence period of up to 6 mo prior to transplantation is mandatory, alcohol relapse after transplantation is a common event. In case of recurrence of heavy drinking, graft survival is significantly impaired. Guidelines on detection and surveillance of alcohol consumption in this patient cohort are lacking. This review summarizes the challenge of patient selection as well as the current knowledge on established and novel alcohol biomarkers with special focus on liver transplant candidates and recipients.

Vapor-Liquid Equilibrium of Ethyl Acetate+C_nH_(2n+1)OH(n=1,2,3) Binary Systems at 0.3 MPa

Vapor-liquid equilibrium data for the binary systems of ethyl acetate + methanol,ethyl acetate + ethanol and ethyl acetate + 1-propanol at 0.3 MPa were determined.The experimental data were verified with the point-to-point test of van Ness(1973).All these systems present an azeotropic point at 0.3 MPa that increases in ester composition for longer alcohol chains.The UNIFAC in different versions and ASOG prediction models were applied.

The Detection of 1-Palmitoyl-2-oleoyl-<i>sn</i>-glycero-3-phosphoethanol and Ethyl Glucuronide in Human Umbilical Cord

In utero exposure to ethanol continues to be a significant public health issue and neonatal healthcare professionals are in need of objective means to identify exposed newborns. The aim of this study was to fully validate two methods for the detection of two direct alcohol biomarkers, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanol (POPE) and ethyl glucuronide (EtG), in umbilical cord and apply the assays to a group of authentic specimens. The limits of detections were 2 and 1 ng/g for POPE and ETG and the limits of quantitation were 4 and 3 ng/g, respectively. Inter and intra-day precision and accuracy measurements were within 15%. The assays were applied to 308 authentic specimens where we detected POPE in five (1.6%) specimens and EtG in twelve (3.9%) specimens. The mean concentrations were 11.4 ng/g ± 9.4 ng/g and 127.2 ± 227.7 ng/g for POPE and EtG, respectively. This study suggested that umbilical cord was a suitable specimen type for the identification of newborns exposed to ethanol in the womb and the prevalence of POPE and EtG detected in umbilical cord were consistent with the prevalence of self-reported binge drinking reported by the National Birth Defect Prevention Study (NBDPS) and Behavioral Risk Factor Surveillance System (BRFSS). Further studies are required to fully describe the association between the observed concentrations of POPE and EtG in umbilical cord to the level of maternal consumption of ethanol.

膨胀止血材料在战创伤急救中的应用探索

目的探讨膨胀止血材料在战创伤急救中的应用价值。方法体外物理性能评价中,将聚乙烯醇(PVA)和聚醚酯-氨基甲酸乙酯(PEEC)分别置于生理盐水、抗凝兔血及生理盐水与抗凝兔血1∶1混合液中,记录2种膨胀止血材料的体积、重量变化及体积压缩一半时所需压强。体内止血性能评价中,构建大鼠股动静脉损伤出血模型和兔肝损伤出血模型,观察无菌纱布、PVA和PEEC 3种止血材料对股动静脉损伤出血和肝损伤出血的止血效果;观察记录术后1 h和术后24 h总出血量、取出止血材料后1 min出血量、取出止血材料时粘连程度和难易程度。结果在3种模拟体液中PVA的体积变化系数、体积变化速率均大于PEEC,重量变化系数小于PEEC(P均<0.01),在生理盐水和抗凝兔血中PVA体积压缩一半所需的压强小于PEEC体积压缩一半所需的压强(P均<0.05)。术后1 h,2个动物模型实验中取出PVA时的粘连程度和取出难易程度均优于无菌纱布和PEEC;取出止血材料后1 min内仅无菌纱布组动物见少量出血,PVA组、PEEC组动物均未见活动性出血。在股动静脉损伤出血模型中PVA组的术后1 h总出血量低于PEEC组(P<0.01),在肝损伤出血模型中术后1 h总出血量PVA组高于PEEC组(P<0.01)。术后24 h,2个动物模型实验中取出PVA时的粘连程度和取出难易程度均优于无菌纱布,PEEC大部分被降解,无法取出;取出止血材料后1 min内,无菌纱布、PVA、PEEC组动物均未见活动性出血。在股动静脉损伤出血模型中无菌纱布组与PVA组的24 h总出血量差异无统计学意义(P>0.05),在肝损伤出血模型中PVA组的24 h总出血量高于无菌组纱布组(P<0.01),在2个动物模型中PEEC组术后24 h总出血量均见少量出血,无法计算出血量。在股动静脉损伤出血模型中,PVA组止血材料附近肌肉组织周边浸润的炎症细胞少于无菌纱布组和PEEC组。结论PVA和PEEC均可通过吸收包括血液在内的体液使自身体积膨大控制股动静脉损伤出血和肝损伤出血,为战创伤救治新装备的研发提供了新的选择。

基于铁死亡和自噬研究酒精的致肝脏细胞损伤作用

目的:探索不同浓度酒精作用不同时间对肝细胞、肝星状细胞和肝母细胞瘤细胞铁死亡和自噬的作用及机制。方法:采用不同浓度(0~800 mmol/L)酒精处理AML12小鼠肝细胞株24、48和72 h,处理JS-1小鼠肝星状细胞株和HepG2人肝母细胞瘤细胞株24和48 h;CCK-8法检测细胞活力;油红O染色检测细胞脂质沉积;乳酸脱氢酶(LDH)释放测定细胞损伤水平;Western blot检测细胞活化相关蛋白α-平滑肌肌动蛋白(α-SMA),细胞因子转化生长因子β1(TGF-β1),胶原沉积相关蛋白Ⅰ型胶原(Col Ⅰ),铁死亡相关蛋白溶质载体家族7成员11(SLC7A11)、谷胱甘肽过氧化物酶4(GPX4)和铁蛋白重链1(FTH1),以及自噬相关蛋白微管相关蛋白1轻链3(LC3)蛋白水平。结果:(1)酒精抑制AML12细胞活力与时间的延长和浓度的升高相关,脂质沉积呈浓度和时间依赖性增加(P<0.05),酒精作用24 h后LDH释放显著增多(P<0.01),FTH1和SLC7A11蛋白表达在24 h显著下调(P<0.01),LC3-Ⅱ/LC3-Ⅰ比值随时间延长而升高(P<0.01)。(2)50、100和150 mmol/L酒精处理24 h后JS-1细胞活力增强,而酒精处理48 h后细胞活力则受到显著抑制;50和100 mmol/L酒精处理JS-1细胞24和48 h后TGF-β1、α-SMA和SLC7A11蛋白表达均显著上调(P<0.05),各浓度酒精处理后Col Ⅰ蛋白表达均显著上调(P<0.01),GPX4和FTH1表达及LC3-Ⅱ/LC3-Ⅰ比值随时间延长而降低(P<0.01)。(3)酒精对HepG2细胞活力的抑制作用随时间延长而增强,细胞脂质沉积呈浓度和时间依赖性增加(P<0.01),FTH1和GPX4蛋白表达随时间延长而下调(P<0.01),随酒精浓度增加而下调(P<0.05),酒精处理组SCL7A11蛋白表达和LC3-Ⅱ/LC3-Ⅰ比值与对照组相比均显著降低。结论:(1)酒精抑制AML12细胞活力时,铁死亡减弱,自噬增强。(2)低浓度酒精通过增强自噬促进JS-1细胞的活化增殖,而高浓度酒精抑制其活化增殖,且增强SLC7A11下调所致的铁死亡;酒精处理的JS-1细胞自噬强度随时间延长而减弱,铁死亡则依赖时间积累而增强。(3)酒精通过铁死亡增强和自噬受损而抑制HepG2细胞活力和促进脂质沉积。