Simultaneous Determination of 14 β-Receptor Agonists Residues in Mutton by High Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS)

[Objectives]A high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method was established for the determination of 14β-receptor agonist residues in mutton.[Methods]Samples were hydrolyzed byβ-glucuronidase and extracted with 5%acetic acid-acetonitrile(1:99,V/V)solution.An Eclipse plus C 18 column was used for separation,and the MRM mode was used for qualitative analysis,and the external standard method was used for quantitative analysis of matrix standard solutions.[Results]Under the optimal conditions,the retention time of the 14 kinds ofβ-receptor agonists ranged from 1.0 to 9.5 min.When the mass concentration was in the range of 0.05-0.50μg/ml,the linear relationship ofβ-receptor agonists was good,with correlation coefficients(r)≥0.9992.The detection limits of the method were in the range of 0.04-0.87μg/kg,and the quantitative limits were in the range of 0.35-1.86μg/kg.The average recovery values were in the range of 82.8%-108.9%,with RSDs(n=6)in the range of 1.9%-6.7%.[Conclusions]The method is simple,sensitive,reproducible,accurate,and can be used for simultaneous determination of the 14 kinds ofβ-receptor agonist residues in mutton.

穴位三部曲联合康复训练对逼尿肌反射亢进型神经源性膀胱尿动力学的影响

目的 探讨穴位三部曲联合康复训练对逼尿肌反射亢进型神经源性膀胱尿动力学、尿道外括约肌肌电图及相关并发症的影响。方法 选择安徽医科大学附属六安医院2019年2月至2023年2月收治的52例逼尿肌反射亢进型神经源性膀胱患者为研究对象。采用随机数字表法分为对照组(26例)予常规间歇导尿护理联合康复训练,观察组(26例)在对照组的基础上采用穴位三部曲,比较两组干预前后的尿动力学、尿道外括约肌肌电图、并发症。结果 干预后,两组最大尿流率、膀胱顺应性、膀胱容量高于干预前,且观察组高于对照组,差异有统计学意义(P<0.05);干预后,两组最大尿流速率时逼尿肌压力低于干预前,且观察组低于对照组,差异有统计学意义(P<0.05)。干预后,两组肌道外括约肌肌电图检测的排尿前振幅值、常用对数TL值高于干预前,且观察组高于对照组,差异有统计学意义(P<0.05)。干预后,两组排尿中振幅值低于干预前,且观察组低于对照组,差异有统计学意义(P<0.05)。观察组并发症总发生率低于对照组,差异有统计学意义(P<0.05)。结论 艾灸、推拿、穴位贴敷三部曲联合康复训练有助于改善逼尿肌亢进型神经源性膀胱患者的尿动力学,提高膀胱顺应性,降低尿路感染及损伤等相关并发症发生率。

帕立骨化醇治疗血液透析患者继发性甲状旁腺功能亢进的临床研究

目的 探讨血液透析继发甲状旁腺功能亢进患者予帕立骨化醇治疗的效果。方法 选取丹阳市第三人民医院于2020年1月—2022年12月诊治的72例血液透析继发甲状旁腺功能亢进患者为研究对象,以随机数表法分为两组,对照组(n=36)予骨化三醇治疗,观察组(n=36)予帕立骨化醇治疗,治疗12周后评估临床疗效,比较治疗前后钙磷代谢及甲状旁腺素水平,并评估炎症反应状态。结果 观察组总有效率较对照组高,差异有统计学意义(P<0.05)。治疗后观察组血钙(2.31±0.24)mmol/L、血磷(1.84±0.22)mmol/L、甲状旁腺素(365.25±84.61)pg/ml均低于对照组,差异有统计学意义(t=3.635、4.605、7.409,P均<0.05)。治疗后观察组炎症因子水平低于对照组,差异有统计学意义(P<0.05)。结论 对血液透析继发性甲状旁腺功能亢进患者,予帕立骨化醇治疗效果确切,可改善钙磷代谢与甲状旁腺素水平,能减轻炎症反应。

Determination of Ten Kinds of Alpha-2 Agonists Residues in Animal Derived Food by UHPLC-Triple Quadrupole/Composite Linear Ion Trap Mass Spectrometry

[Objectives]The paper was to establish an ultra high performance liquid chromatography-quadrupole/linear ion trap complex mass spectrometry for the determination of 10 kinds ofα2-receptor agonists in animal derived food.[Methods]The samples were extracted with sodium carbonate buffer solution and ethyl acetate,and analyzed by mass spectrometry after solid phase extraction and high performance liquid chromatography separation.[Results]Ten kinds ofα2-receptor agonists showed a good linear relationship in the range of 1-100μg/mL,with the average recovery of over 69%and the relative standard deviation less than 8.32%.The detection limit of 10 kinds of α_(2)-receptor agonists was up to 1μg/kg.[Conclusions]The method has good selectivity and strong anti-interference ability,and can meet the requirements of 10 kinds ofα2-receptor agonists residues in animal derived food.

下斜肌转位联合水平直肌手术治疗下斜肌亢进合并V型斜视的临床研究

目的 探讨下斜肌转位联合水平直肌手术矫正下斜肌亢进合并V型斜视的有效性及安全性。方法 选取2020年11月至2021年9月浙江中医药大学附属嘉兴中医院收治的下斜肌亢进合并V型斜视患者39例为研究对象,根据手术方案不同将其分为联合手术组(n=28)和分次手术组(n=11)。比较两组患者的视力、眼位、V征治愈率、下斜肌亢进矫正率、眼心反射、眼胃反射及并发症。结果 术后1个月,两组患者均恢复第一眼位正位,且V征治愈数和下斜肌亢进矫正数比较差异均无统计学意义(P>0.05),两组患者的视力均恢复至术前水平;两组患者的眼心反射、眼胃反射发生情况比较差异均无统计学意义(P>0.05);两组患者均无炎症反应、结膜下大量出血、眼睑皮下血肿、球后血肿等并发症。结论 下斜肌转位联合水平直肌手术矫正下斜肌亢进合并V型斜视的疗效不劣于常规分次手术,但可减少手术次数,避免二次手术给患者带来的痛苦,值得临床推广应用。

Experimental Study on AT1-receptor-peptide-induced Myocardial Immune Damage in Rat

In order to investigate the immunological damage in rat immunized with AT1-receptor peptide, 18 male Wistar rats were divided into two groups: immunized-group (n=12), each rat was immunized with 150 μg AT 1-receptor petide coupled to bovine serum albumin, together with Freund's adjuvant. Control group (n=6), sham-immunized, 'immunized liquid' was same as immunized-group except AT1-receptor peptide. Systolic blood pressure (SBP) was measured by using the tail-cuff technique, antibody against AT1-receptor peptide detected by using ELISA method, and left ventricular myocardium and renal cortex sections were observed under light and electron microscopy. There was no significant difference in SBP and light microscopic observation of the tissue sections between the immunized-group and control group. The O.D. value of anti-AT1-receptor peptide antiserum was significantly higher in the immunized-group than in the rats before immunization and control group (P<0.01). Positive rate in the immunized-group was 100 %, while 0 % in the control group. Ultramicroscopic morphology showed potential myocardial injury, including: increase in number of mitochondria, swelling of many mitochondria with reduction in number or absence of their cristae and cristolysis, disorder of the cardiac myofibrils, and myofibrillar disruption and myocytolysis. And lysosomes were increased in renal tubular epithelia. The AT1-receptor peptide could induce to generate the antibody against AT1-receptor peptide and lead to myocardial and renal damage in rats.

Efficacy of the H2-receptor antagonist famotidine on chronic spontaneous urticaria in children

Urticaria is a common pediatric skin disorder. Histamine H1-receptor antagonists are effective in chronic as well as acute urticaria. When H1-anti-histamines are ineffective, add-on use of H2-receptor antagonists is thought to give better symptom relief. However, there are few reports on the therapeutic efficacy in pediatric patients. We retrospectively reviewed the medical records of pediatric patients with chronic spontaneous urticaria (csU) who met the following criteria. They were consulted our outpatient clinic between April 2010 and March 2012;were unsuccessfully treated with H1 antihistamines;and were treated with add-on H2-receptor antagonist (famotidine). In six patients who met the inclusion criteria (mean age 6.1 ± 5.1 years), urticaria activity score was significantly decreased from 4.3 ± 0.8 just before administration of famotidine to 1.3 ± 1.0 on the first outpatient visit within 4 weeks after the first administration of famotidine

Electronic Structure of some A3 Adenosine-Receptor Antagonist——A Structure Activity Relationship

DFT quantum chemical computations have been carried out at the B3LYP/6-31G (d) level. Full geometry optimization has been performed and equilibrium geometries for a new series of phenyl thiazoles have been located. Ground state electronic properties, charge density distributions, dipole moments and its components have been calculated and reported. Effect of substituents on the geometry and on the polarization of the studied series of compounds are analyzed and discussed. Some structural features have been pinpointed to underline the affinity and selectivity of the studied compounds as adenosine A3-receptor antagonists. Results of the present work indicate that activity towards A3 receptor sites is directly correlated with both of the polarity and the co-planarity of the thiazole.

下斜肌减弱处理对水平肌加强减弱术患儿视功能、下斜肌功能和创伤恢复的影响

目的分析在水平肌加强减弱术基础上开展下斜肌减弱术治疗儿童斜视伴下斜肌功能亢进的效果。方法选择2019年12月—2021年12月本院收治的64例斜视合并下斜肌功能亢进患儿作为研究对象,随机数表法分为对照组(行水平肌加强减弱术+下斜肌后徙术)和观察组(行水平肌加强减弱术+下斜肌减弱术)两组,每组各32例。记录两组的围术期指标(术中出血量、伤口恢复时间、手术时间),记录术前和术后1个月、3个月垂直斜视角、水平斜视角;评估斜视疗效、下斜肌功能亢进疗效,记录术前和术后3个月两组视功能分级,记录并发症。结果与对照组比较,观察组术中出血量更少,伤口恢复时间更短;术后1-3个月两组垂直斜视角和水平斜视角均降低,且观察组低于对照组;观察组斜视治疗有效率(96.88%)、下斜肌功能亢进治疗有效率(93.75%)均高于对照组(71.88%、71.88%),上述比较差异均有统计学意义(P<0.05)。两组手术时间以及术前和术后3个月视功能分级比较,差异无统计学意义(P>0.05)。结论水平肌加强减弱术+下斜肌减弱术用于儿童斜视伴下斜肌功能亢进治疗中,能改善视功能,进一步提高斜视和下斜肌功能亢进疗效,创伤小,伤口恢复快,安全性高。

Parathyroid carcinoma:Three case reports

BACKGROUND Parathyroid carcinoma(PC)is a rare,slow-growing malignant tumor and a rare cause of primary hyperfunctioning of the parathyroid,with a highly variable clinical course,depending on the aggressiveness of the individual tumor and the degree of hypercalcemia.CASE SUMMARY The aim of this report is to summarize the diagnosis and treatment of three cases of PC and to review and conclude aspects regarding the three collected cases with reference to other relevant cases to explore the value of ultrasound in the diagnosis of PC.All three patients had hypercalcemia,consisting of a high serum calcium level and a high level of parathyroid hormone that was>2-fold(even>30-fold)of the normal upper limit.The ultrasonographic findings of the parathyroid gland showed that the glands were all>30 mm,and the internal echo was uneven.All patients underwent surgery.PC in three cases was confirmed by routine histopathology and immunohistochemistry.CONCLUSION As clinical signs and laboratory results are nonspecific,it is difficult to diagnose PC preoperatively,so imaging examinations are often needed.

围手术期护理在脾动脉栓塞术、经腹全子宫结合双侧输卵管切除术中的应用效果

目的探讨围手术期护理在脾动脉栓塞术、经腹全子宫结合双侧输卵管切除术中的应用效果。方法选取本院收治的60例子宫肌瘤合并脾功能亢进需要进行脾动脉栓塞术、经腹全子宫结合双侧输卵管切除术的患者为研究对象,根据入院就诊号分为两组,各30例。对照组(奇数)采用常规护理,观察组(偶数)采用围手术期护理,比较两组的心理状态、生活质量及护理质量。结果护理后,观察组焦虑自评量表、抑郁自评量表评分得分低于对照组,差异有统计学意义(P<0.05);护理后,观察组社会功能、生理机能、日常活动功能、活力得分高于对照组,差异有统计学意义(P<0.05);观察组基础护理、健康教育、技术操作、风险预防意识、自主服务意识得分高于对照组,差异有统计学意义(P<0.05)。结论针对子宫肌瘤合并脾功能亢进需要进行脾动脉栓塞术、经腹全子宫结合双侧输卵管切除术的患者,开展围手术期护理,效果显著。

A Prospective, Multicentric, Post Marketing Surveillance to Evaluate Efficacy & Safety of Ranitidine HCl (150 & 300 mg IR/CR) in Indian Patients with Gastroesophageal Reflux Disease (PROGRADE)

Purpose: Ranitidine hydrochloride (HCl) remains an important medication for treating acid-peptic ailments such as Gastroesophageal reflux disease (GERD). The main objective of this Post Marketing Surveillance (PMS) clinical study was to test the efficacy and safety of Ranitidine HCl in Indian patients suffering from GERD. Patients and Methods: Data of 2446 patients (1307 males;1121 females) from 21 centers across India were analyzed. Patients received either of the three treatments: Ranitidine HCl 150 mg twice a day (BID) (ARM-A), Ranitidine HCl 300 mg once daily (OD) or BID (ARM-B), and Ranitidine HCl 300 mg OD (ARM-C). Gastroesophageal Reflux Disease Symptom Assessment Scale (GSAS) score and Heartburn Severity score were used to assess the drug’s efficacy. The adverse events reported by patients or investigators were analyzed to assess the safety profile of Ranitidine. Results: Of the 2446 subjects screened, 2428 were enrolled. There was a significant reduction in GSAS scores from baseline to the end of the study visit in all three ARMs. The GSAS scores reduced from 2.02 to 0.23 in ARM-A, 2.01 to 0.24 in ARM-B, and 2.07 to 0.26 in ARM-C patients. In ARM A, 72.82% had 24 hours heartburn-free days, and 66.89% had 7 consecutive heartburn-free days, which was more significant than the other two ARMs. 128 (5.27%) patients reported ADRs due to Ranitidine HCl at different doses. The most frequently reported ADR was constipation (17.18%), followed by oliguria (14.06%), cold (13.28%), and dysuria (12.5%). Of 128 ADRs, 113 (88.28%) were mild, and only 11 (8.59%) ADRs were related to the study drug. No severe ADRs were reported during the study. Conclusion: Ranitidine HCl 150/300 mg tablet was found to be an effective and safe H2-receptor antagonist for treating GERD in Indian Patients.

夏枯草颗粒联合甲巯咪唑片治疗毒性弥漫性甲状腺肿伴甲状腺功能亢进症患者临床研究

目的:观察夏枯草颗粒联合甲巯咪唑片治疗毒性弥漫性甲状腺肿伴甲状腺功能亢进症患者的疗效及对血清成纤维细胞生长因子21(FGF21)、促甲状腺素受体抗体(TR-Ab)水平的影响。方法:选择78例毒性弥漫性甲状腺肿伴甲状腺功能亢进患者为研究对象,随机分为对照组与观察组各39例。对照组接受甲巯咪唑片治疗,观察组在对照组基础上联合夏枯草颗粒治疗。比较2组临床疗效,比较2组治疗前后血清游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺素(TSH)及FGF21、TR-Ab水平。结果:治疗前,2组血清FT3、FT4、TSH水平比较,差异无统计学意义(P>0.05);治疗后,2组血清FT3、FT4水平均降低(P<0.05),TSH水平升高(P<0.05),且观察组FT3、FT4水平低于对照组,TSH水平高于对照组(P<0.05)。治疗前,2组血清FGF21、TR-Ab水平比较,差异无统计学意义(P>0.05);治疗后,2组血清FGF21水平升高(P<0.05),TR-Ab水平降低(P<0.05),且观察组FGF21水平高于对照组(P<0.05),TR-Ab水平低于对照组(P<0.05)。治疗后,观察组治疗总有效率92.31%,高于对照组76.92%(P<0.05)。结论:夏枯草颗粒联合甲巯咪唑片治疗毒性弥漫性甲状腺肿伴甲状腺功能亢进,在改善甲状腺功能方面比单用甲巯咪唑更有优势,且可以调节血清FGF21、TR-Ab水平,提高临床疗效。

环磷酰胺致大鼠免疫抑制和免疫亢进模型的建立与评价

目的用环磷酰胺(cyclophosphamide,Cy)建立大鼠免疫抑制和免疫亢进模型,并从免疫器官、免疫细胞、抗体和细胞因子等方面对该模型进行评价。方法 SD大鼠随机分为A、B、C三大组,所有大鼠腹腔注射100μg鸡卵清白蛋白(ovalbumin,OVA)进行免疫,B组大鼠于免疫OVA后6 h,以不同的方式腹腔注射不同剂量的Cy,C组大鼠于免疫OVA前3 d,以不同的方式腹腔注射不同剂量的Cy,A组为对照组。结果免疫OVA后6 h,一次性注射不同剂量Cy(125、100 mg/kg),能使大鼠产生明显的免疫抑制,免疫OVA前3 d注射不同剂量Cy,超大剂量时(225 mg/kg)依然会引起动物的免疫抑制,小剂量(20 mg/kg)一次注射或者更小剂量(每天5 mg/kg,连续3d)注射在不同时间点均能引起机体免疫亢进。结论为免疫抑制和免疫亢进模型的建立提供方法和数据支持具有重要参考意义。

β受体功能亢进症儿童立位与卧位同步12导联心电图T波及ST段变化的意义

目的探讨β受体功能亢进症儿童立位与卧位同步12导联心电图（12 ECG）T波及ST段变化的意义。方法β受体功能亢进症儿童（研究组）53例。男13例,女40例;年龄3.5~18.0（8.77±2.99）岁。广东中山SR-1000A心电综合自动分析仪描记卧位及立位12 ECG。匹配健康儿童40例为健康对照组。计算立位与卧位心率差及T波、ST段振幅及形态变化。采用SPSS 11.0软件进行统计学分析。结果1.研究组发病年龄高峰为7~11岁。男女比例约为1：3。2.研究组立位较卧位心率增加幅度显著高于健康对照组[（17.96±10.16）次/minvs（9.67±6.97）次/min P〈0.01]。3.T波形态：研究组立位与卧位比较,T波振幅降低主要在Ⅱ、V3、V4、V5、V6导联,T波倒置主要在Ⅲ、aVF导联,T波倒置且振幅增加主要在Ⅲ、V1、V2导联,卧位时T波倒置变为立位时T波直立出现在V1、V2导联。4.T波振幅：研究组立位较卧位T波振幅下降幅度在V1导联大于健康对照组（P〈0.05）,在V2导联显著大于健康对照组（P〈0.01）。5.ST段振幅：立位较卧位变化在研究组及健康对照组间无统计学差异（P〉0.05）。结论儿童β受体功能亢进症发病年龄高峰在学龄期,尤以女性多见,心率明显增加,立位和卧位心电图T波形态和振幅变化明显。

1000例广州地区儿童中医体质调查研究

目的研究广州地区儿童中医体质分布规律,从而为儿童体质调理提供理论依据。方法采用中医体质分类与判定标准,制定《小儿中医体质及健康状况自填式问卷》(家长或监护人填写),并结合舌脉以分析判定儿童中医体质类型。结果调查结果显示平和质占4.5%,阴虚质占50.3%,气虚质占35.2%,痰湿质占20.2%,实热质占67.8%,特禀质占6.1%。其中2种以上兼夹体质占调查对象的85.6%,而兼夹体质中气阴两虚质占24.1%,脾虚肝旺质占33.7%,气虚痰湿质占14.8%,脾虚湿热质占13%。而3岁及3岁以下小儿兼夹体质分布中气阴两虚占26.3%,脾虚肝旺质占24.8%,气虚痰湿质占10.9%,脾虚湿热质占8.9%;3岁以上小儿气阴两虚质占22.6%,脾虚肝旺质占39.7%,气虚痰湿质占17.4%,脾虚湿热质占4.5%。结论广州地区儿童单纯中医体质以实热质、阴虚质、气虚质为主,且无年龄差异;但以2种以上兼夹体质为主,且兼夹体质分布具有年龄差异,其中3岁及3岁以下小儿以气阴两虚质为主,3岁以上小儿以脾虚肝旺质为主。对于3岁以上儿童,除了体质调养之外,应注意心理健康引导。

针灸联合抑亢汤治疗多囊卵巢综合征疗效及对卵巢储备功能的影响

目的分析针灸联合抑亢汤治疗多囊卵巢综合征对患者卵巢储备功能的影响。方法选取医院于2016年2月—2018年8月收治的80例多囊卵巢综合征患者的临床资料进行分析,分为两组。对照组采用抑亢汤治疗,观察组采用针灸联合抑亢汤治疗,分析两组患者治疗后的临床疗效。结果两组患者治疗前中医证候评分组间比较,差异不具有统计学意义(P>0.05)。观察组患者治疗后月经稀发、崩漏、婚久不孕、胸肋胀痛、面部痤疮评分低于对照组(P<0.05)。两组患者治疗前性激素水平、B超检查结果组间比较,差异不具有统计学意义(P>0.05)。观察组患者治疗后雌二醇(E2)水平高于对照组,促黄体生成素(LH)、睾酮(T)水平、卵巢体积、卵泡数低于对照组,但两组患者卵泡刺激素(FSH)水平、子宫体积比较,差异不具有统计学意义(P>0.05)。观察组月经恢复30例、排卵29例、妊娠25例,发生率分别为75.00%(30/40)、72.50%(29/40)、62.50%(25/40),对照组月经恢复28例、排卵25例、妊娠14例,发生率分别为70.00%(28/40)、62.50%(25/40)、35.00%(14/40)。但两组月经恢复、排卵发生情况比较,差异不具有统计学意义(P>0.05)。结论针灸联合抑亢汤治疗多囊卵巢综合征,可改善患者的临床症状、子宫情况及卵巢储备功能,提高妊娠率。

D-二聚体阳性患者在临床科室的分布及其检测临床意义

目的探讨D-二聚体阳性患者在临床各科室的分布及其临床意义。方法选取该院收治的1 003例患者,采用胶乳增强免疫比浊法对其血浆D-二聚体水平进行检测。结果 ICU科、心内科、呼吸内科、骨科、普外科、肝病科、神经外科、妇产科和肿瘤科患者总阳性率为44.1%。同时,弥散性血管内凝血(DIC)、深静脉血栓、肺动脉栓塞、心脑血管疾病、肝脏疾病和恶性肿瘤患者中的D-二聚体阳性者分别为86、34、26、24、18、12例。结论血浆D-二聚体水平的检测可用于血栓性疾病的预防、监控。