为指导辐照熟食加工厂完善HACCP质量管理体系方案,本文阐述了食品危害分析和关键控制点(hazard analysis critical control point,HACCP)质量管理体系工作原理及流程、γ射线辐照灭菌原理及过程。以某熟食食品厂火腿生产工艺为例,通过...为指导辐照熟食加工厂完善HACCP质量管理体系方案,本文阐述了食品危害分析和关键控制点(hazard analysis critical control point,HACCP)质量管理体系工作原理及流程、γ射线辐照灭菌原理及过程。以某熟食食品厂火腿生产工艺为例,通过鱼骨图绘制出熟食火腿在生产、辐照至出厂过程中的控制点与关键控制点,着重分析辐照灭菌部分。通过研究辐照食品的控制点及关键控制点,制定熟食辐照HACCP计划表,确定辐照食品在辐照过程中的关键控制点为辐照实施过程中剂量的控制。推广HACCP质量管理体系的建立,对食品生产厂家的可持续发展起到至关重要的作用,完善HACCP质量管理体系中关于食品进行γ射线辐照灭菌的过程体系文件,能够有效指导和监控食品辐照灭菌流程,提高食品的安全性,保障饮食安全,有必要在食品生产管理领域推广。展开更多
Our previous studies have shown that zein has good biocompatibility and good mechanical properties. The first product from a porous scaffold of zein, a resorbable bone substitute, has passed the biological evaluation ...Our previous studies have shown that zein has good biocompatibility and good mechanical properties. The first product from a porous scaffold of zein, a resorbable bone substitute, has passed the biological evaluation of medical devices （ISO 10993） by the China Food and Drug Administration. However, Class III medical devices need quality monitoring before being placed on the market, and such monitoring includes quality control of raw materials, choice of sterilization method, and evaluation of biocompatibility. In this paper, we investigated four sources of zein through amino acid analysis （AAA） and sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS-PAGE） in order to monitor the composition and purity, and control the quality of raw materials. We studied the effect of three kinds of sterilization method on a porous zein scaffold by SDS-PAGE. We also compared the changes in SDS-PAGE patterns when irradiated with different doses of gamma radiation. We found that polymerization or breakage did not occur on peptide chains of zein during gamma-ray （y-ray） sterilization in the range of 20-30 kGy, which suggested that γ-ray sterilization is suitable for porous zein scaffolds, Regarding cell compatibility, we found a difference between using a 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide （MTT） assay and a cell-counting kit-8 （CCK-8） assay to assess cell proliferation on zein film, and concluded that the CCK-8 assay is more suitable, due to its low background optical density.展开更多
文摘目的:建立天麻药材指纹图谱,考察不同剂量60Co-γ射线辐照灭菌对天麻药材中天麻素、对羟基苯甲醇、巴利森苷A、巴利森苷B、柠檬酸5个有效成分含量的影响。方法:利用HPLC法,采用wondasil C18(250 mm×4.6 mm,5μm)液相色谱柱,以乙腈-0.1%磷酸溶液为流动相,梯度洗脱;流速为1.0 ml·min^(-1);检测波长为220 nm;柱温为30℃,进样量为10μl,建立天麻药材HPLC指纹图谱。分别选择辐照剂量2,6,10 k Gy对天麻进行辐照,比较辐照前后天麻指纹图谱及5个有效成分的变化,并采用SPSS 22.0软件分析5个有效成分辐照前后含量变化。结果:共确定14个共有峰,不同剂量辐照前后天麻药材指纹图谱的相似度为0.918~0.999;辐照剂量不超过6 k Gy时,天麻药材中的天麻素、对羟基苯甲醇、巴利森苷A、巴利森苷B、柠檬酸含量变化无统计学意义(P>0.05)。结论:所建立的天麻指纹图谱及5个成分含量测定方法可作为天麻质控方法,在辐照剂量不超过6 k Gy时,60Co-γ辐照灭菌法可用于天麻药材的灭菌。
文摘为指导辐照熟食加工厂完善HACCP质量管理体系方案,本文阐述了食品危害分析和关键控制点(hazard analysis critical control point,HACCP)质量管理体系工作原理及流程、γ射线辐照灭菌原理及过程。以某熟食食品厂火腿生产工艺为例,通过鱼骨图绘制出熟食火腿在生产、辐照至出厂过程中的控制点与关键控制点,着重分析辐照灭菌部分。通过研究辐照食品的控制点及关键控制点,制定熟食辐照HACCP计划表,确定辐照食品在辐照过程中的关键控制点为辐照实施过程中剂量的控制。推广HACCP质量管理体系的建立,对食品生产厂家的可持续发展起到至关重要的作用,完善HACCP质量管理体系中关于食品进行γ射线辐照灭菌的过程体系文件,能够有效指导和监控食品辐照灭菌流程,提高食品的安全性,保障饮食安全,有必要在食品生产管理领域推广。
文摘Our previous studies have shown that zein has good biocompatibility and good mechanical properties. The first product from a porous scaffold of zein, a resorbable bone substitute, has passed the biological evaluation of medical devices （ISO 10993） by the China Food and Drug Administration. However, Class III medical devices need quality monitoring before being placed on the market, and such monitoring includes quality control of raw materials, choice of sterilization method, and evaluation of biocompatibility. In this paper, we investigated four sources of zein through amino acid analysis （AAA） and sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS-PAGE） in order to monitor the composition and purity, and control the quality of raw materials. We studied the effect of three kinds of sterilization method on a porous zein scaffold by SDS-PAGE. We also compared the changes in SDS-PAGE patterns when irradiated with different doses of gamma radiation. We found that polymerization or breakage did not occur on peptide chains of zein during gamma-ray （y-ray） sterilization in the range of 20-30 kGy, which suggested that γ-ray sterilization is suitable for porous zein scaffolds, Regarding cell compatibility, we found a difference between using a 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide （MTT） assay and a cell-counting kit-8 （CCK-8） assay to assess cell proliferation on zein film, and concluded that the CCK-8 assay is more suitable, due to its low background optical density.
