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舒芬太尼超前镇痛在喉癌气管切开中的应用 被引量:2
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作者 武毅彬 朱毅 《山西医药杂志》 CAS 2014年第12期1410-1412,共3页
超前镇痛是指阻止外周损伤冲动传向中枢,在伤害刺激作用于机体之前通过给予一些药物来防止中枢神经敏化达到消除或减轻术后疼痛,并可以减少术后镇痛药物用量[1]。阿片类药物作用于μ受体,以超前镇痛的方式有效地阻止痛觉过敏的发生。舒... 超前镇痛是指阻止外周损伤冲动传向中枢,在伤害刺激作用于机体之前通过给予一些药物来防止中枢神经敏化达到消除或减轻术后疼痛,并可以减少术后镇痛药物用量[1]。阿片类药物作用于μ受体,以超前镇痛的方式有效地阻止痛觉过敏的发生。舒芬太尼是芬太尼的N-4位衍生物,主要作用于μ阿片受体。其亲脂性约为芬太尼的2倍,对其临床应用已有大量报道,但其用于超前镇痛方面的研究还比较少见。喉癌手术是头颈外科的常见手术类型。 展开更多
关键词 超前镇痛 术后镇痛药物 喉癌手术 痛觉过敏 气管切开 伤害刺激 术后疼痛 手术类型 阿片类药物 μ受
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Human μ-opioid receptor overexpressed in Sf9 insect cells functionally coupled to endogenous G_(i/o) proteins 被引量:3
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作者 WEI QIANG DE HE ZHOU +5 位作者 QING XIANG SHEN JIE CHEN LI WEI CHEN TIE LIN WANG GANG PEI ZHI QIANG CHI(e-mail:dhzhou@mail.shcnc.ac.cn)(1 Shanghai Institute of Materia Medica,2 Shanghai Institute of Cell Biology,Shanghai Academy of Life Sciences, Chinese Acad 《Cell Research》 SCIE CAS CSCD 2000年第2期93-102,共10页
Human μ-opioid receptor (HμOR) with a tag of six consecutive histidines at its carboxyl terminus had been expressed in recombinant baculovirus infected Sf9 insect cells.The maximal binding capacity for the [3H] di... Human μ-opioid receptor (HμOR) with a tag of six consecutive histidines at its carboxyl terminus had been expressed in recombinant baculovirus infected Sf9 insect cells.The maximal binding capacity for the [3H] diprenorphine and [3H]ohmefentanyl (Ohm) were 9.1± 0.7 and 6.52±0.23 nmol/g protein, respectively. The [3H] diprenorphine or [3H] Ohm binding to the receptor expressed in Sf9 cells was strongly inhibited by μ-selective agonists [D-Ala2], N-methylPhe4, glyol5]enkephalin (DAGO), Ohm, and morphine, but neither by δ nor by K selective agonist. Na+ (100 mM) and GTP (50 μM) could reduce HμOR agonists etorphine and Ohm affinity binding to the overexpressed HμOR. μ-selective agonists DAGO and Ohm effectively stimulated [35S]GTPγS binding (EC50 = 2.7nM and 6.9 nM) and inhibited forskolin- stimulated cAMP accumulation (IC50 = 0.9 nM and 0.3 nM). The agonist-dependent effects could be blocked by opioid antagonist naloxone or by pretreatment of cells with pertussis toxin (PTX). These results demonstrated that HμOR overexpressed in Sf9 insect cells functionally coupled to endogenous Gi/o proteins. 展开更多
关键词 Human μ-opioid receptor (HμOR) Sf9 insect cells pertussis toxin (PTX) endogenous G_(i/o) proteins
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Changes of mu and kappa opioid receptors in cathartic colon of rat
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作者 刘宝华 莫平 +2 位作者 贾后军 李春穴 张胜本 《Journal of Medical Colleges of PLA(China)》 CAS 2004年第5期282-284,共3页
Objective: To observe the changes of mu and kappa opioid receptors in the cathartic colon of rat, and to clarify that whether opioid receptors accounts for the occurrence of slow transit constipation (STC). Methods: T... Objective: To observe the changes of mu and kappa opioid receptors in the cathartic colon of rat, and to clarify that whether opioid receptors accounts for the occurrence of slow transit constipation (STC). Methods: The cathartic colon model of rat was made by feeding with laxatives. The activity of mu and kappa opioid receptors in the cathartic colon of rat was measured by radio-ligand binding assay. Results: Compared with the control group, the maximal binding capacity (Bmax) and affinity(Kd) of mu opioid receptor in cathartic colon group were significantly increased (207.00±22.90 fmol/mg·p vs 82.00±14.23 fmol/mg·p, P < 0.01;3.30±0.45 mmol/L vs 2.40±0.57 mmol/L,P < 0.05). The maximal binding capacity of kappa opioid receptor also showed a great increase (957.00±102.41 fmol/mg·p vs 459.00±52.41 fmol/mg·p, P<0.01), but no significant difference of affinity was found between the two groups. Conclusion: The mu and kappa opioid receptors may be involved in the functional disorders of cathartic colon. 展开更多
关键词 cathartic colon mu opioid receptor kappa opioid receptor
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