Enantiomer of n-pyrrolidinyl phenylpropanol was studied by capillary electrophoresis using b-cyclodextrin polymer as chiral selector. We determined the enantiomeric excess value of n-pyrrolidinyl phenylpropanol with R...Enantiomer of n-pyrrolidinyl phenylpropanol was studied by capillary electrophoresis using b-cyclodextrin polymer as chiral selector. We determined the enantiomeric excess value of n-pyrrolidinyl phenylpropanol with RSD 0.48%.展开更多
Ahn To establish an RP-HPLC method for determination of content of 1-phenylpropanol in its raw material and preparations. Methods Chromatography was carried out on a Dikma DiamonsilTM ODS column, using a mobile phase ...Ahn To establish an RP-HPLC method for determination of content of 1-phenylpropanol in its raw material and preparations. Methods Chromatography was carried out on a Dikma DiamonsilTM ODS column, using a mobile phase of methanol-water （55：45） with a flow rate at 1.0 mL·min^-1. The detection wavelength was 258 nm. Results Under the chromatographic condition, the peaks of 1-phenylpropanol and its related impurities separated completely; noninterference between the principal agent and adjuvants in preparations was performed. The calibration curve was linear over the range of 250 - 750μg·mL^-1 with the correlation coefficient of 0. 999 9. The average recovery was 100.2% （ RSD = 1.35% ）. Conclusion This method is simple, rapid, accurate, specific, and can be used to detect the content and related compounds of phenylpropanol in its raw material and preparations.展开更多
Aim Isolation and structural elucidation of the constituents from the aerial part of Vitis thunbergii . Methods To isolate chemical constituents, column chromatography and HPLC were used. Physico chemical ...Aim Isolation and structural elucidation of the constituents from the aerial part of Vitis thunbergii . Methods To isolate chemical constituents, column chromatography and HPLC were used. Physico chemical characterization and spectroscopic analysis were employed for structural identification. Results Eleven polyphenols were isolated and identified. Conclusion Compound 1 is a new compound and its structure was characterized to be 3,5 dimethoxyl 4 hydroxyl phenylpropanol 9 O β D glycopyranoside.展开更多
将胰蛋白酶酶解牛血清白蛋白产生的多肽与环糊精键合,用羰基咪唑法键合到硅胶上,得到新型多肽键合环糊精硅胶手性固定相.反相液相色谱法拆分手性化合物利胆醇,考察了流动相的pH梯度变化时间、离子强度以及流速对利胆醇手性拆分的影响,...将胰蛋白酶酶解牛血清白蛋白产生的多肽与环糊精键合,用羰基咪唑法键合到硅胶上,得到新型多肽键合环糊精硅胶手性固定相.反相液相色谱法拆分手性化合物利胆醇,考察了流动相的pH梯度变化时间、离子强度以及流速对利胆醇手性拆分的影响,探讨了该新型多肽键合环糊精硅胶手性柱对利胆醇的手性识别机理及利胆醇在该柱上的手性拆分重复性.实验表明:流动相组成为60 mol/L磷酸盐缓冲液;pH梯度为1 min 5.6,10 min 6.4;流速为0.5 mL/min时分离效果最佳.展开更多
文摘Enantiomer of n-pyrrolidinyl phenylpropanol was studied by capillary electrophoresis using b-cyclodextrin polymer as chiral selector. We determined the enantiomeric excess value of n-pyrrolidinyl phenylpropanol with RSD 0.48%.
文摘Ahn To establish an RP-HPLC method for determination of content of 1-phenylpropanol in its raw material and preparations. Methods Chromatography was carried out on a Dikma DiamonsilTM ODS column, using a mobile phase of methanol-water （55：45） with a flow rate at 1.0 mL·min^-1. The detection wavelength was 258 nm. Results Under the chromatographic condition, the peaks of 1-phenylpropanol and its related impurities separated completely; noninterference between the principal agent and adjuvants in preparations was performed. The calibration curve was linear over the range of 250 - 750μg·mL^-1 with the correlation coefficient of 0. 999 9. The average recovery was 100.2% （ RSD = 1.35% ）. Conclusion This method is simple, rapid, accurate, specific, and can be used to detect the content and related compounds of phenylpropanol in its raw material and preparations.
文摘Aim Isolation and structural elucidation of the constituents from the aerial part of Vitis thunbergii . Methods To isolate chemical constituents, column chromatography and HPLC were used. Physico chemical characterization and spectroscopic analysis were employed for structural identification. Results Eleven polyphenols were isolated and identified. Conclusion Compound 1 is a new compound and its structure was characterized to be 3,5 dimethoxyl 4 hydroxyl phenylpropanol 9 O β D glycopyranoside.
文摘将胰蛋白酶酶解牛血清白蛋白产生的多肽与环糊精键合,用羰基咪唑法键合到硅胶上,得到新型多肽键合环糊精硅胶手性固定相.反相液相色谱法拆分手性化合物利胆醇,考察了流动相的pH梯度变化时间、离子强度以及流速对利胆醇手性拆分的影响,探讨了该新型多肽键合环糊精硅胶手性柱对利胆醇的手性识别机理及利胆醇在该柱上的手性拆分重复性.实验表明:流动相组成为60 mol/L磷酸盐缓冲液;pH梯度为1 min 5.6,10 min 6.4;流速为0.5 mL/min时分离效果最佳.
