AIM:To investigate the expression of thymidylate synthase(TS)and glutathione-s-transferaseπ(GST-π) in esophageal squamous cell carcinoma and their association with the clinicopathologic characteristics. METHODS:Immu...AIM:To investigate the expression of thymidylate synthase(TS)and glutathione-s-transferaseπ(GST-π) in esophageal squamous cell carcinoma and their association with the clinicopathologic characteristics. METHODS:Immunohistochemical methods were used to detect the expression of TS and GST-πin surgically resected formalin-fixed,paraffin-embedded esophageal squamous cell carcinoma(ESCC)tissue sections from 102 patients(median age,58 years)and in 28 normal esophageal mucosa(NEM)samples.The relationship between TS and GST-πexpression and clinicopathologic factors was examined. RESULTS:The expression of TS and GST-πwas not statistically significantly associated with age of the patients,tumor size,lymph node metastasis,depth of invasion or tumor stage.TS staining was positive in 17.86%of normal esophageal mucosa and in 42.16% of ESCC samples(P<0.05).The expression level of TSwas not only significantly lower in well-differentiated (21.88%)than in poorly-differentiated carcinomas (51.43%,P<0.05),but was also significantly higher in samples from male patients(46.51%)than from female patients(18.75%,P<0.05).GST-πwas positively stained in 78.57%of normal esophageal mucosa and in 53.92%of ESCC samples(P<0.05).The expression level of GST-πwas also significantly higher in welldifferentiated carcinomas(65.63%)than in poorlydifferentiated carcinomas(35.00%,P<0.05). CONCLUSION:The expression of TS and of GST-πmay be used as molecular markers for the characterization of ESCC.Poorly-differentiated cells showed increased expression of TS and reduced expression of GST-π.展开更多
Three novel diketopyrrolopyrrole （DPP） based small organic molecules were synthesized as hole transporting materials for perovskite solar cells. The effects of different donors and zr bridges on the performance of p...Three novel diketopyrrolopyrrole （DPP） based small organic molecules were synthesized as hole transporting materials for perovskite solar cells. The effects of different donors and zr bridges on the performance of perovskite solar cells （PSCs） were discussed. The efficiency of TPADPP-1, TPADPP-2. PTZDPP-2 was 5.10%, 9.85% and 8.16% respectively. Compared to TPADPP-2, the voltage of PTZDPP-2 was higher. Because the electron-donatingability of phenothiazine based donor was larger than that of triphenylamine based donor, the HOMO level of PTZDPP-2 was lower than that of TPADPP-2. The results indicated that the diketopyrrolopyrrole based D-π-A-π-D type small organic molecule might be a promising hole trans- porting material in the perovskite solar cells.展开更多
Objective :To investigate the influence of GST πgenetransferintohumancord blood hematopoie ticstem cellson their drugres is tanceagains tanti tumordrugs invivo .Methods: GST πgenetran sfectionin to human cord blood ...Objective :To investigate the influence of GST πgenetransferintohumancord blood hematopoie ticstem cellson their drugres is tanceagains tanti tumordrugs invivo .Methods: GST πgenetran sfectionin to human cord blood CD 34+cells was carried outusing are trovirusvec torPLJ GST πwith the aido f fibronect in .Succes sfulgenetransfer was confirmed by invitrocolony assay and RT PCR .GST πgenetrans duced human cord blood CD34+cells were the nengrafted in to 4 week old total body irradiated NOD/Scid mice and carboplatin was intraperitoneally admin is tered sequentially at 4 weeks interval 4 week saftereng raftment.Results :Peripheral blood (PB) WBC was significantly higher in GST πmice than control mice after 2 course of car boplat in .Retroviral GST π expression in bone marrow hematopoietic progenitor cells of recipient mice was detected by RT PCR 16 weeks after Xenotransplantation .Conclusion :The transfection of GST π gene could confer ,to some extent ,resistance to cord blood stem cells against carboplatin in vivo .展开更多
Efficient heterojunction organic photovoltaic （OPV） cells are fabricated based on copper tetra-methyl phthalocyanine （CuMePc） as donor and fullerene （C60） as acceptor. The power conversion efficiency of CuMePc/C...Efficient heterojunction organic photovoltaic （OPV） cells are fabricated based on copper tetra-methyl phthalocyanine （CuMePc） as donor and fullerene （C60） as acceptor. The power conversion efficiency of CuMePc/C60 OPV cell （2.52%） is increased by 88% compared with that of the non-peripheral substituted copper phthalocyanine （CuPc）/C60 OPV cell （1.34%）. The introduction of methyl substituent leads to stronger π–π interaction of CuMePc （～ 3.5 ？） than that of CuPc （～ 3.8 ？）. The efficiency improvement is attributed to the enhanced carrier mobility of CuMePc thin film （1.1×10-3 cm2/V·s） and better film morphology by introducing methyl groups into the periphery of CuPc molecule.展开更多
基金Supported by The Key Medical Talent Foundation of Jiangsu Province,China,No.RC2007036
文摘AIM:To investigate the expression of thymidylate synthase(TS)and glutathione-s-transferaseπ(GST-π) in esophageal squamous cell carcinoma and their association with the clinicopathologic characteristics. METHODS:Immunohistochemical methods were used to detect the expression of TS and GST-πin surgically resected formalin-fixed,paraffin-embedded esophageal squamous cell carcinoma(ESCC)tissue sections from 102 patients(median age,58 years)and in 28 normal esophageal mucosa(NEM)samples.The relationship between TS and GST-πexpression and clinicopathologic factors was examined. RESULTS:The expression of TS and GST-πwas not statistically significantly associated with age of the patients,tumor size,lymph node metastasis,depth of invasion or tumor stage.TS staining was positive in 17.86%of normal esophageal mucosa and in 42.16% of ESCC samples(P<0.05).The expression level of TSwas not only significantly lower in well-differentiated (21.88%)than in poorly-differentiated carcinomas (51.43%,P<0.05),but was also significantly higher in samples from male patients(46.51%)than from female patients(18.75%,P<0.05).GST-πwas positively stained in 78.57%of normal esophageal mucosa and in 53.92%of ESCC samples(P<0.05).The expression level of GST-πwas also significantly higher in welldifferentiated carcinomas(65.63%)than in poorlydifferentiated carcinomas(35.00%,P<0.05). CONCLUSION:The expression of TS and of GST-πmay be used as molecular markers for the characterization of ESCC.Poorly-differentiated cells showed increased expression of TS and reduced expression of GST-π.
基金the National Key Research Development Program of China(2016YFA0602900)the National Natural Science Foundation of China(21272079,21572069)+1 种基金the Science and Technology Planning Project of Guangdong Province,China(2013B010405003)the fund from the Guangzhou Science and Technology Project,China(201607010265)
文摘Three novel diketopyrrolopyrrole （DPP） based small organic molecules were synthesized as hole transporting materials for perovskite solar cells. The effects of different donors and zr bridges on the performance of perovskite solar cells （PSCs） were discussed. The efficiency of TPADPP-1, TPADPP-2. PTZDPP-2 was 5.10%, 9.85% and 8.16% respectively. Compared to TPADPP-2, the voltage of PTZDPP-2 was higher. Because the electron-donatingability of phenothiazine based donor was larger than that of triphenylamine based donor, the HOMO level of PTZDPP-2 was lower than that of TPADPP-2. The results indicated that the diketopyrrolopyrrole based D-π-A-π-D type small organic molecule might be a promising hole trans- porting material in the perovskite solar cells.
文摘Objective :To investigate the influence of GST πgenetransferintohumancord blood hematopoie ticstem cellson their drugres is tanceagains tanti tumordrugs invivo .Methods: GST πgenetran sfectionin to human cord blood CD 34+cells was carried outusing are trovirusvec torPLJ GST πwith the aido f fibronect in .Succes sfulgenetransfer was confirmed by invitrocolony assay and RT PCR .GST πgenetrans duced human cord blood CD34+cells were the nengrafted in to 4 week old total body irradiated NOD/Scid mice and carboplatin was intraperitoneally admin is tered sequentially at 4 weeks interval 4 week saftereng raftment.Results :Peripheral blood (PB) WBC was significantly higher in GST πmice than control mice after 2 course of car boplat in .Retroviral GST π expression in bone marrow hematopoietic progenitor cells of recipient mice was detected by RT PCR 16 weeks after Xenotransplantation .Conclusion :The transfection of GST π gene could confer ,to some extent ,resistance to cord blood stem cells against carboplatin in vivo .
基金Project supported by the Special Funds for the Development of Strategic Emerging Industries in Shenzhen City,China(Grant No.JCYJ20120830154526537)Start-up Funding of the South University of Science and Technology of China,and the Strategic Research Grant of the City University of Hong Kong(Grant No.7002724)
文摘Efficient heterojunction organic photovoltaic （OPV） cells are fabricated based on copper tetra-methyl phthalocyanine （CuMePc） as donor and fullerene （C60） as acceptor. The power conversion efficiency of CuMePc/C60 OPV cell （2.52%） is increased by 88% compared with that of the non-peripheral substituted copper phthalocyanine （CuPc）/C60 OPV cell （1.34%）. The introduction of methyl substituent leads to stronger π–π interaction of CuMePc （～ 3.5 ？） than that of CuPc （～ 3.8 ？）. The efficiency improvement is attributed to the enhanced carrier mobility of CuMePc thin film （1.1×10-3 cm2/V·s） and better film morphology by introducing methyl groups into the periphery of CuPc molecule.
