Lipases have been widely applied in a variety of industrial fields,such as food,pharmaceuticals,biofuels,and biotechnology.Recent years have witnessed a great interest in modifying lipids for the production of triacyl...Lipases have been widely applied in a variety of industrial fields,such as food,pharmaceuticals,biofuels,and biotechnology.Recent years have witnessed a great interest in modifying lipids for the production of triacylglycerols enriched with n-3 polyunsaturated fatty acids(PUFAs).Here,a novel salt-tolerant,organic solvent-stable,and bile salt-activated lipase was purified from golden pompano(Trachinotus ovatus)viscera,which was named as golden pompano lipase(GPL).GPL had a specific activity of 57.2U mg^(-1)with an estimated molecular weight of 14 k Da,exhibited optimal activity at 40℃a nd pH 8.0,and showed K_(m)and V_(max)of 40.16μmol L^(-1)and 769.23μmol L^(-1)min^(-1),respectively.GPL activity was enhanced by Mn^(2+)and sodium deoxycholate.It was active in organic solvents,including methanol,ethanol,chloroform,and hexane.GPL also showed a good salinity tolerance of up to 1 mol L^(-1).n-3PUFA enrichment in the glyceride fraction of golden pompano oil was performed by GPL-catalyzed hydrolysis and yielded a total PUFA concentration of 56.99%.EPA,DHA,and DPA were enriched by 10.4-,3.2-,and 1.8-fold of their initial levels,respectively.This study recognized the industrial applicability of GPL to prepare enriched C_(20-22)n-3 PUFA.展开更多
以茯砖茶为菌源，分离筛选出20株具有3-苯氧基苯甲酸（3-PBA）降解能力的“金花菌”，其中分离的菌株ET1可在7d内完全降解PD培养基中100mg／L的3-PBA，根据其形态特征和ITS序列分析（Gen Bank Accession No．KF317836），将其鉴定为冠...以茯砖茶为菌源，分离筛选出20株具有3-苯氧基苯甲酸（3-PBA）降解能力的“金花菌”，其中分离的菌株ET1可在7d内完全降解PD培养基中100mg／L的3-PBA，根据其形态特征和ITS序列分析（Gen Bank Accession No．KF317836），将其鉴定为冠突散囊菌（Eurotiumcristatum）。动力学研究表明，菌株ET1降解3-PBA的过程符合一级动力学方程，在测试的3-PBA质量浓度、温度、pH值范围内，3-PBA半衰期为1．7～3．2d，远远低于其自然降解半衰期（180d），且该反应的速率受pH值影响较大，受3-PBA质量浓度和温度的影响相对较小。展开更多
广电运通进入全球ATM前十强，德利多富获全球金融发展论坛殊荣，爱立信率先在3GPP定义的所有频段上实现WCDMA呼叫，讯宝科技移动终端帮助GOLDEN STATE OVERNIGHT为客户提供可靠的服务，德州仪器将大规模生产Gen 2芯片，NCR率先通过EMV4....广电运通进入全球ATM前十强，德利多富获全球金融发展论坛殊荣，爱立信率先在3GPP定义的所有频段上实现WCDMA呼叫，讯宝科技移动终端帮助GOLDEN STATE OVERNIGHT为客户提供可靠的服务，德州仪器将大规模生产Gen 2芯片，NCR率先通过EMV4.l.a Level 2认证，展开更多
基金This work was supported by the National Key R&D Programs of China(No.2018YFD0901103)the Program of the Hainan Association for Science and Technology Plans to Youth R&D Innovation(No.QCXM202003)the Hainan Provincial Natural Science Foundation of China(No.2019RC093).
文摘Lipases have been widely applied in a variety of industrial fields,such as food,pharmaceuticals,biofuels,and biotechnology.Recent years have witnessed a great interest in modifying lipids for the production of triacylglycerols enriched with n-3 polyunsaturated fatty acids(PUFAs).Here,a novel salt-tolerant,organic solvent-stable,and bile salt-activated lipase was purified from golden pompano(Trachinotus ovatus)viscera,which was named as golden pompano lipase(GPL).GPL had a specific activity of 57.2U mg^(-1)with an estimated molecular weight of 14 k Da,exhibited optimal activity at 40℃a nd pH 8.0,and showed K_(m)and V_(max)of 40.16μmol L^(-1)and 769.23μmol L^(-1)min^(-1),respectively.GPL activity was enhanced by Mn^(2+)and sodium deoxycholate.It was active in organic solvents,including methanol,ethanol,chloroform,and hexane.GPL also showed a good salinity tolerance of up to 1 mol L^(-1).n-3PUFA enrichment in the glyceride fraction of golden pompano oil was performed by GPL-catalyzed hydrolysis and yielded a total PUFA concentration of 56.99%.EPA,DHA,and DPA were enriched by 10.4-,3.2-,and 1.8-fold of their initial levels,respectively.This study recognized the industrial applicability of GPL to prepare enriched C_(20-22)n-3 PUFA.
文摘以茯砖茶为菌源，分离筛选出20株具有3-苯氧基苯甲酸（3-PBA）降解能力的“金花菌”，其中分离的菌株ET1可在7d内完全降解PD培养基中100mg／L的3-PBA，根据其形态特征和ITS序列分析（Gen Bank Accession No．KF317836），将其鉴定为冠突散囊菌（Eurotiumcristatum）。动力学研究表明，菌株ET1降解3-PBA的过程符合一级动力学方程，在测试的3-PBA质量浓度、温度、pH值范围内，3-PBA半衰期为1．7～3．2d，远远低于其自然降解半衰期（180d），且该反应的速率受pH值影响较大，受3-PBA质量浓度和温度的影响相对较小。
