A protocol is presented for genetically engineering loblolly pine (Pinus taeda L.) using particle bombardment. This protocol enabled the routine transformation of loblolly pine plants that were previously difficult to...A protocol is presented for genetically engineering loblolly pine (Pinus taeda L.) using particle bombardment. This protocol enabled the routine transformation of loblolly pine plants that were previously difficult to transform. Mature zygotic embryos were used to be bombarded and to generate organogenic callus and transgenic regenerated plants. Plasmid pB48.215 DNA contained a synthetic Bacillus thuringiensis (B.t.) cryIAc coding sequence flanked by the double cauliflower mosaic virus (CaMV) 35S promoter and nopaline synthase (Nos) terminator sequences, and the selectable marker gene, neomycin phosphotransferase II (nptII) controlled by the promoter of the nopaline synthase gene was introduced into loblolly pine tissues by particle bombardment. The transformed tissues were proliferated and selected by kanamycin resistance conferred by the introduced NPTII gene. Shoot regeneration was induced from the kanamycin-resistant callus, and transgenic plantlets were then produced. The presence of the introduced genes in the transgenic loblolly pine plants was confirmed by polymerase chain reactions (PCR) analysis, by Southern blot analysis, and insect feeding assays. The recovered transgenic plants were acclimatized and then established in soil.展开更多
The study aims to ascertain the hypothesis on the rich rotifer biodiversity of the floodplain lakes （beels） of the Brahmaputra river basin and to use these metazoans to assess trophic status or to characterize habit...The study aims to ascertain the hypothesis on the rich rotifer biodiversity of the floodplain lakes （beels） of the Brahmaputra river basin and to use these metazoans to assess trophic status or to characterize habitat variations of wetlands. The plankton samples collected from four beels of lower Assam revealed 160 Rotifera species belonging to 35 genera and 19 families. The richness is of biodiversity value as -38.0% and -57.0% of the rotifer species known till date from India and northeast India （NEI）, respectively. One species each is new to the Oriental region and NEI, and three species are new to Assam; 23 species merit global biogeography interest and several exhibit distribution values in the Indian sub-region. The diverse Lecanidae 〉 Brachionidae 〉 Lepadellidae 〉 Trichocercidae and speciose littoral-periphytic Lecane〉 Lepadella〉Trichocerca, and richness of Brachionus spp. following removal of aquatic macrophytes are noteworthy. Overall rotifer composition showed homogeneity amongst beels while lower monthly richness and community similarities affirmed heterogeneity within individual beels. We propose L/B quotient based on Lecane： Brachionus species ratios to characterize habitat variations of the sampled wetlands. Slfide^ek＇s B/T quotient based on Brachionus： Trichocerca species ratios affirmed general ＇meso-trophic＇ status of different beels. Our results provided little insight on the influence of individual abiotic factors but the canonical correspondence analyses asserted higher cumulative influence of ten abiotic parameters on Rotifera richness in each beel.展开更多
Let B be a Banach space in UMD with an unconditional basis. The boundedness of the θ (t)_type singular integral operators in L p B(R n),(1≤p<+∞) and H 1 B(R n) spaces are discussed.
文摘A protocol is presented for genetically engineering loblolly pine (Pinus taeda L.) using particle bombardment. This protocol enabled the routine transformation of loblolly pine plants that were previously difficult to transform. Mature zygotic embryos were used to be bombarded and to generate organogenic callus and transgenic regenerated plants. Plasmid pB48.215 DNA contained a synthetic Bacillus thuringiensis (B.t.) cryIAc coding sequence flanked by the double cauliflower mosaic virus (CaMV) 35S promoter and nopaline synthase (Nos) terminator sequences, and the selectable marker gene, neomycin phosphotransferase II (nptII) controlled by the promoter of the nopaline synthase gene was introduced into loblolly pine tissues by particle bombardment. The transformed tissues were proliferated and selected by kanamycin resistance conferred by the introduced NPTII gene. Shoot regeneration was induced from the kanamycin-resistant callus, and transgenic plantlets were then produced. The presence of the introduced genes in the transgenic loblolly pine plants was confirmed by polymerase chain reactions (PCR) analysis, by Southern blot analysis, and insect feeding assays. The recovered transgenic plants were acclimatized and then established in soil.
文摘The study aims to ascertain the hypothesis on the rich rotifer biodiversity of the floodplain lakes （beels） of the Brahmaputra river basin and to use these metazoans to assess trophic status or to characterize habitat variations of wetlands. The plankton samples collected from four beels of lower Assam revealed 160 Rotifera species belonging to 35 genera and 19 families. The richness is of biodiversity value as -38.0% and -57.0% of the rotifer species known till date from India and northeast India （NEI）, respectively. One species each is new to the Oriental region and NEI, and three species are new to Assam; 23 species merit global biogeography interest and several exhibit distribution values in the Indian sub-region. The diverse Lecanidae 〉 Brachionidae 〉 Lepadellidae 〉 Trichocercidae and speciose littoral-periphytic Lecane〉 Lepadella〉Trichocerca, and richness of Brachionus spp. following removal of aquatic macrophytes are noteworthy. Overall rotifer composition showed homogeneity amongst beels while lower monthly richness and community similarities affirmed heterogeneity within individual beels. We propose L/B quotient based on Lecane： Brachionus species ratios to characterize habitat variations of the sampled wetlands. Slfide^ek＇s B/T quotient based on Brachionus： Trichocerca species ratios affirmed general ＇meso-trophic＇ status of different beels. Our results provided little insight on the influence of individual abiotic factors but the canonical correspondence analyses asserted higher cumulative influence of ten abiotic parameters on Rotifera richness in each beel.
文摘Let B be a Banach space in UMD with an unconditional basis. The boundedness of the θ (t)_type singular integral operators in L p B(R n),(1≤p<+∞) and H 1 B(R n) spaces are discussed.
