The genetic diversities of 110 pummelo germplasms and 12 of their relatives were analyzed by SSR and AFLP methods. Approximately 99.1% of the 335 SSR loci were polymorphic, and 9.85 alleles per SSR locus were identifi...The genetic diversities of 110 pummelo germplasms and 12 of their relatives were analyzed by SSR and AFLP methods. Approximately 99.1% of the 335 SSR loci were polymorphic, and 9.85 alleles per SSR locus were identified. The gene diversity values changed from 0.1939 to 0.9073, and 46 SSR polymorphic bands were scored. 72% of the 343 AFLP loci were polymorphic, and 82 polymorphic loci per AFLP were identified. Heterozygosity changed from 0.21863 to 0.28445, and 44 AFLP polymorphic bands were scored. The UPGMA result showed that 122 pummelo genotypes and their relatives could be divided into eight groups, and the pummelo genotypes composed mainly of Shatian pummelo varieties group, Wendan pummelo vareties group and a huge hybrid pummelo varieties group. The classification result was expected to widen the genetic background of pummelos using various target varieties.展开更多
以易发生汁胞粒化的老龄树和不易发生汁胞粒化的适龄树的琯溪蜜柚(Citrus grand is(L.) Osbeck‘Guanxi-miyou’)果实为材料,研究了果实成熟过程中汁胞粒化发生与细胞壁代谢的关系。结果表明:老龄树果实的汁胞粒化指数随着果实成熟而上...以易发生汁胞粒化的老龄树和不易发生汁胞粒化的适龄树的琯溪蜜柚(Citrus grand is(L.) Osbeck‘Guanxi-miyou’)果实为材料,研究了果实成熟过程中汁胞粒化发生与细胞壁代谢的关系。结果表明:老龄树果实的汁胞粒化指数随着果实成熟而上升。在汁胞粒化发生过程中,汁胞维持较低的细胞壁降解酶[果胶甲酯酶(PE)、多聚半乳糖醛酸酶(PG)、纤维素酶(Cx)]活性,保持较高的细胞壁物质(原果胶、纤维素、半纤维素)含量;尤其在汁胞粒化的起动阶段和加快阶段,纤维素、半纤维素含量极显著增加。相反,适龄树果实的汁胞粒化指数在果实成熟过程中变化不大,汁胞中细胞壁降解酶活性较高,促进原果胶、纤维素、半纤维素等细胞壁物质的降解,保持较低的细胞壁物质含量,使汁胞发育正常、柔软多汁。这说明PE、PG、Cx活性和原果胶、纤维素、半纤维素含量与琯溪蜜柚汁胞粒化密切相关。展开更多
【目的】探讨酸雨胁迫对‘琯溪蜜柚’叶片抗氧化酶活性和光合作用的影响。【方法】以p H值5.6为对照,采用p H 2.5和p H 4.5模拟酸雨对1 a(年)生‘琯溪蜜柚’进行胁迫,研究酸雨对蜜柚叶片氧化伤害、抗氧化酶系统、气体交换参数和叶片结...【目的】探讨酸雨胁迫对‘琯溪蜜柚’叶片抗氧化酶活性和光合作用的影响。【方法】以p H值5.6为对照,采用p H 2.5和p H 4.5模拟酸雨对1 a(年)生‘琯溪蜜柚’进行胁迫,研究酸雨对蜜柚叶片氧化伤害、抗氧化酶系统、气体交换参数和叶片结构的影响。【结果】p H 2.5和p H 4.5模拟酸雨胁迫处理3个月,没有造成叶片可见伤害,叶片上表皮细胞排列整齐紧密,但p H 2.5模拟酸雨胁迫处理显著降低了叶肉中栅栏组织发育;p H 2.5模拟酸雨胁迫处理1 d后,叶片SOD和CAT活性的升高减少O2-和H2O2的积累,降低过氧化伤害,p H 2.5模拟酸雨胁迫处理3 d之后引起叶片膜脂过氧化;p H 4.5模拟酸雨胁迫处理6 d后虽然3种抗氧化系统酶活性显著高于对照,但不足以清除酸雨胁迫引起的活性氧,引起膜脂过氧化;模拟酸雨胁迫处理初期,2种处理均显著抑制光合速率,酸雨处理90 d后,p H 2.5酸雨显著抑制‘琯溪蜜柚’叶片光合速率,推测是由于氧化胁迫、栅栏组织发育等非气孔调节因素引起的,p H 4.5酸雨处理下叶片具有较强自我修复能力,其光合速率没有受到显著抑制。【结论】‘琯溪蜜柚’具有较强的酸雨抗性,p H 4.5中度酸雨胁迫对植株生长影响不显著,p H 2.5重度酸雨胁迫显著抑制叶片光合作用。展开更多
基金The project was financed by the National Natural Science Foundation of China(NSFC30070528)the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of Ministry of Education,P.R.China.We are thankful to the Citrus Research Institute,Chinese Academy of Agricultural Sciences+1 种基金Horticultural Institute,Jiangxi Academy of Agricultural Sciencesand Pummelo Germplasms Repository of Meixian County,Guangdong Province for providing part of plant materials.
文摘The genetic diversities of 110 pummelo germplasms and 12 of their relatives were analyzed by SSR and AFLP methods. Approximately 99.1% of the 335 SSR loci were polymorphic, and 9.85 alleles per SSR locus were identified. The gene diversity values changed from 0.1939 to 0.9073, and 46 SSR polymorphic bands were scored. 72% of the 343 AFLP loci were polymorphic, and 82 polymorphic loci per AFLP were identified. Heterozygosity changed from 0.21863 to 0.28445, and 44 AFLP polymorphic bands were scored. The UPGMA result showed that 122 pummelo genotypes and their relatives could be divided into eight groups, and the pummelo genotypes composed mainly of Shatian pummelo varieties group, Wendan pummelo vareties group and a huge hybrid pummelo varieties group. The classification result was expected to widen the genetic background of pummelos using various target varieties.
文摘以易发生汁胞粒化的老龄树和不易发生汁胞粒化的适龄树的琯溪蜜柚(Citrus grand is(L.) Osbeck‘Guanxi-miyou’)果实为材料,研究了果实成熟过程中汁胞粒化发生与细胞壁代谢的关系。结果表明:老龄树果实的汁胞粒化指数随着果实成熟而上升。在汁胞粒化发生过程中,汁胞维持较低的细胞壁降解酶[果胶甲酯酶(PE)、多聚半乳糖醛酸酶(PG)、纤维素酶(Cx)]活性,保持较高的细胞壁物质(原果胶、纤维素、半纤维素)含量;尤其在汁胞粒化的起动阶段和加快阶段,纤维素、半纤维素含量极显著增加。相反,适龄树果实的汁胞粒化指数在果实成熟过程中变化不大,汁胞中细胞壁降解酶活性较高,促进原果胶、纤维素、半纤维素等细胞壁物质的降解,保持较低的细胞壁物质含量,使汁胞发育正常、柔软多汁。这说明PE、PG、Cx活性和原果胶、纤维素、半纤维素含量与琯溪蜜柚汁胞粒化密切相关。
文摘【目的】探讨酸雨胁迫对‘琯溪蜜柚’叶片抗氧化酶活性和光合作用的影响。【方法】以p H值5.6为对照,采用p H 2.5和p H 4.5模拟酸雨对1 a(年)生‘琯溪蜜柚’进行胁迫,研究酸雨对蜜柚叶片氧化伤害、抗氧化酶系统、气体交换参数和叶片结构的影响。【结果】p H 2.5和p H 4.5模拟酸雨胁迫处理3个月,没有造成叶片可见伤害,叶片上表皮细胞排列整齐紧密,但p H 2.5模拟酸雨胁迫处理显著降低了叶肉中栅栏组织发育;p H 2.5模拟酸雨胁迫处理1 d后,叶片SOD和CAT活性的升高减少O2-和H2O2的积累,降低过氧化伤害,p H 2.5模拟酸雨胁迫处理3 d之后引起叶片膜脂过氧化;p H 4.5模拟酸雨胁迫处理6 d后虽然3种抗氧化系统酶活性显著高于对照,但不足以清除酸雨胁迫引起的活性氧,引起膜脂过氧化;模拟酸雨胁迫处理初期,2种处理均显著抑制光合速率,酸雨处理90 d后,p H 2.5酸雨显著抑制‘琯溪蜜柚’叶片光合速率,推测是由于氧化胁迫、栅栏组织发育等非气孔调节因素引起的,p H 4.5酸雨处理下叶片具有较强自我修复能力,其光合速率没有受到显著抑制。【结论】‘琯溪蜜柚’具有较强的酸雨抗性,p H 4.5中度酸雨胁迫对植株生长影响不显著,p H 2.5重度酸雨胁迫显著抑制叶片光合作用。
