This work presents the charge-coupled device(CCD)photometric survey of the old open cluster NGC 188.Timeseries V-band photometric observations were conducted for ten nights in 2017 January using the Nanshan Onemeter W...This work presents the charge-coupled device(CCD)photometric survey of the old open cluster NGC 188.Timeseries V-band photometric observations were conducted for ten nights in 2017 January using the Nanshan Onemeter Wide-field Telescope to search for variable stars in the field of the cluster.A total of 25 variable stars,including one new variable star,were detected in the target field.Among the detected variables,16 are cluster member stars,and the others are identified as field stars.The periods,radial velocities,effective temperatures,and classifications of the detected variables are discussed in this work.Most of the stars’effective temperatures are between 4200 and 6600 K,indicating their spectral types are G or K.The newly discovered variable is probably a W UMa system.In this study,a known cluster variable star(V21=V0769 Cep)is classified as an EA-type variable star based on the presence of an 0.5 mag eclipse in its light curve.展开更多
BACKGROUND Altered miR-188-3p expression has been observed in various human cancers.AIM To investigate the miR-188-3p expression,its roles,and underlying molecular events in gastric cancer.METHODS Fifty gastric cancer...BACKGROUND Altered miR-188-3p expression has been observed in various human cancers.AIM To investigate the miR-188-3p expression,its roles,and underlying molecular events in gastric cancer.METHODS Fifty gastric cancer and paired normal tissues were collected to analyze miR-188-3p and CBL expression.Normal and gastric cancer cells were used to manipulate miR-188-3p and CBL expression through different assays.The relationship between miR-188-3p and CBL was predicted bioinformatically and confirmed using a luciferase gene reporter assay.A Kaplan-Meier analysis was used to associate miR-188-3p or CBL expression with patient survival.A nude mouse tumor cell xenograft assay was used to confirm the in vitro data.RESULTS MiR-188-3p was found to be lower in the plasma of gastric cancer patients,tissues,and cell lines compared to their healthy counterparts.It was associated with overall survival of gastric cancer patients(P<0.001),tumor differentiation(P<0.001),lymph node metastasis(P=0.033),tumor node metastasis stage(I/II vs III/IV,P=0.024),and American Joint Committee on Cancer stage(I/II vs III/IV,P=0.03).Transfection with miR-188-3p mimics reduced tumor cell growth and invasion while inducing apoptosis and autophagy.CBL was identified as a direct target of miR-188-3p,with its expression antagonizing the effects of miR-188-3p on gastric cancer(GC)cell proliferation by inducing tumor cell apoptosis and autophagy through the inactivation of the Akt/mTOR signaling pathway.The in vivo data confirmed antitumor activity via CBL downregulation in gastric cancer.CONCLUSION The current data provides ex vivo,in vitro,and in vivo evidence that miR-188-3p acts as a tumor suppressor gene or possesses antitumor activity in GC.展开更多
基金the Resource sharing platform construction project of Xinjiang Uygur Autonomous Region(No.PT2306)the Chinese Academy of Sciences(CAS)“Light of West China”Program(No.2020-XBQNXZ-016,2022-XBQNXZ-016)。
文摘This work presents the charge-coupled device(CCD)photometric survey of the old open cluster NGC 188.Timeseries V-band photometric observations were conducted for ten nights in 2017 January using the Nanshan Onemeter Wide-field Telescope to search for variable stars in the field of the cluster.A total of 25 variable stars,including one new variable star,were detected in the target field.Among the detected variables,16 are cluster member stars,and the others are identified as field stars.The periods,radial velocities,effective temperatures,and classifications of the detected variables are discussed in this work.Most of the stars’effective temperatures are between 4200 and 6600 K,indicating their spectral types are G or K.The newly discovered variable is probably a W UMa system.In this study,a known cluster variable star(V21=V0769 Cep)is classified as an EA-type variable star based on the presence of an 0.5 mag eclipse in its light curve.
基金Supported by the National Natural Science Funds of China,No.81974448Guangdong Medical Research Foundation,No.B2019126Shenzhen Science and Technology Innovation Commission,No.JCYJ20210324135005013.
文摘BACKGROUND Altered miR-188-3p expression has been observed in various human cancers.AIM To investigate the miR-188-3p expression,its roles,and underlying molecular events in gastric cancer.METHODS Fifty gastric cancer and paired normal tissues were collected to analyze miR-188-3p and CBL expression.Normal and gastric cancer cells were used to manipulate miR-188-3p and CBL expression through different assays.The relationship between miR-188-3p and CBL was predicted bioinformatically and confirmed using a luciferase gene reporter assay.A Kaplan-Meier analysis was used to associate miR-188-3p or CBL expression with patient survival.A nude mouse tumor cell xenograft assay was used to confirm the in vitro data.RESULTS MiR-188-3p was found to be lower in the plasma of gastric cancer patients,tissues,and cell lines compared to their healthy counterparts.It was associated with overall survival of gastric cancer patients(P<0.001),tumor differentiation(P<0.001),lymph node metastasis(P=0.033),tumor node metastasis stage(I/II vs III/IV,P=0.024),and American Joint Committee on Cancer stage(I/II vs III/IV,P=0.03).Transfection with miR-188-3p mimics reduced tumor cell growth and invasion while inducing apoptosis and autophagy.CBL was identified as a direct target of miR-188-3p,with its expression antagonizing the effects of miR-188-3p on gastric cancer(GC)cell proliferation by inducing tumor cell apoptosis and autophagy through the inactivation of the Akt/mTOR signaling pathway.The in vivo data confirmed antitumor activity via CBL downregulation in gastric cancer.CONCLUSION The current data provides ex vivo,in vitro,and in vivo evidence that miR-188-3p acts as a tumor suppressor gene or possesses antitumor activity in GC.
