[Objectives] This study was conducted to analyze the differences in procyanidine content in grapes caused by different producing areas. [Methods]With Muscat Hamburg grape from different producing areas as an experimen...[Objectives] This study was conducted to analyze the differences in procyanidine content in grapes caused by different producing areas. [Methods]With Muscat Hamburg grape from different producing areas as an experimental material,procyanidine content was determined by n-butanol-hydrochloric acid colorimetry. [Results] The procyanidins contents in different parts of the same variety ranked as grape seed > grape skin > grape flesh. The procyanidins contents in grapes from different producing areas were in order of Changli County,Hebei Province > Dazeshan Town,Shandong Province > Hangu District,Tianjin City.[Conclusions]The procyanidins contents in Muscat Hamburg grapes from different producing areas differ to a certain degree.展开更多
Monoterpenes are typical aroma components of muscat grape cultivars,providing pleasant floral and fruity aromas to grapes and wines.However,the molecular mechanism of monoterpene biosynthesis between muscat and non-mu...Monoterpenes are typical aroma components of muscat grape cultivars,providing pleasant floral and fruity aromas to grapes and wines.However,the molecular mechanism of monoterpene biosynthesis between muscat and non-muscat grape remains unclear.Here,the muscat grape cultivar‘Jumeigui’and the non-muscat grape cultivar‘Kyoho’were chosen as plant materials for a comprehensive transcriptome and metabolite analysis.The gas chromatography-mass spectrometry(GC-MS)analysis demonstrated that a total of 27 and 23 monoterpene compounds were identified and quantified in the‘Jumeigui’and‘Kyoho’grape,respectively.‘Jumeigui’grape accumulated significantly higher concentrations of monoterpenes than‘Kyoho’grape.Furthermore,geraniol,linalool,geranic acid,and β-citronellol might be important odorants contributing to the floral character of the‘Jumeigui’grape due to the high levels odor activity values(OAVs).Transcriptome analysis demonstrated that the expression profiles of VvDXS,VvGGPPS.SSU1,Vv TPS-b/g showed a positive correlation with monoterpene accumulation in grapes.In addition,the expression patterns of the genes involved in jasmonic acid(JA)synthesis and signal were also positively correlated with monoterpene accumulation.All these results will help guide the functional verification of candidate genes related to monoterpene biosynthesis,as well as identify the master transcriptional and hormonal regulators of this pathway in grapes.展开更多
基金Supported by the Key Research and Development Foundation of Tianjin City(17YFXTZC00040)Agriculture Product Quality and Security Detection Foundation of Ministery of Agriculture(181821301092362005)Tianjin Science and Technology Program(17JCYBJC29800,16PTZSTG00020)
文摘[Objectives] This study was conducted to analyze the differences in procyanidine content in grapes caused by different producing areas. [Methods]With Muscat Hamburg grape from different producing areas as an experimental material,procyanidine content was determined by n-butanol-hydrochloric acid colorimetry. [Results] The procyanidins contents in different parts of the same variety ranked as grape seed > grape skin > grape flesh. The procyanidins contents in grapes from different producing areas were in order of Changli County,Hebei Province > Dazeshan Town,Shandong Province > Hangu District,Tianjin City.[Conclusions]The procyanidins contents in Muscat Hamburg grapes from different producing areas differ to a certain degree.
基金supported by the National Natural Science Foundation of China(Grant No.32002005)the National Natural Science Foundation of Shandong Province(Grant No.ZR2020QC149)+2 种基金the Breeding Plan of Shandong Provincial Qingchuang Research Team(2019)the science-technology benefiting people project of Qingdao(Grant No.21-1-4-ny-14-nsh)the Highlevel Scientific Research Foundation of Qingdao Agricultural University(Grant Nos.665/1118011,665/1119002)。
文摘Monoterpenes are typical aroma components of muscat grape cultivars,providing pleasant floral and fruity aromas to grapes and wines.However,the molecular mechanism of monoterpene biosynthesis between muscat and non-muscat grape remains unclear.Here,the muscat grape cultivar‘Jumeigui’and the non-muscat grape cultivar‘Kyoho’were chosen as plant materials for a comprehensive transcriptome and metabolite analysis.The gas chromatography-mass spectrometry(GC-MS)analysis demonstrated that a total of 27 and 23 monoterpene compounds were identified and quantified in the‘Jumeigui’and‘Kyoho’grape,respectively.‘Jumeigui’grape accumulated significantly higher concentrations of monoterpenes than‘Kyoho’grape.Furthermore,geraniol,linalool,geranic acid,and β-citronellol might be important odorants contributing to the floral character of the‘Jumeigui’grape due to the high levels odor activity values(OAVs).Transcriptome analysis demonstrated that the expression profiles of VvDXS,VvGGPPS.SSU1,Vv TPS-b/g showed a positive correlation with monoterpene accumulation in grapes.In addition,the expression patterns of the genes involved in jasmonic acid(JA)synthesis and signal were also positively correlated with monoterpene accumulation.All these results will help guide the functional verification of candidate genes related to monoterpene biosynthesis,as well as identify the master transcriptional and hormonal regulators of this pathway in grapes.
