To provide ＂more reasonable, more saving and more efficient＂ water and fertilizer application proposals, taking ‘Yujiao 5＇ as the experimental material, the effects of different irrigation times and nitrogen appli...To provide ＂more reasonable, more saving and more efficient＂ water and fertilizer application proposals, taking ‘Yujiao 5＇ as the experimental material, the effects of different irrigation times and nitrogen application treatments on the leaf area index and yield of wheat were studied using three-factor split plot method. The results showed that irrigation times, nitrogen application rate and the ratio of basa to topdressed nitrogen respectively had significant effects on the leaf area index, the yield and component factors of wheat. Under the treatment of W1（irrigation before sowing）, the leaf area index showed a positive linear correlation with nitrogen application rate; under the treatments of W2（irrigation before sowing and at jointing stage） and W3（irrigation before sowing, at jointing stage and at grain filling stages）,the leaf area index showed a positive linear correlation with nitrogen application rate at the jointing stage, booting stage and heading stage; 20 d after heading, the leaf area index showed a quadric curve relationship with nitrogen application rate at these stages, and the LAI of N3R2 was the highest. Under different irrigation times,the yield, ear number and kernels per ear showed quadric curve relationship with nitrogen application rate, 1 000-seed weight showed the trend of linear decrease with the increase of nitrogen application rate. Under the treatment combination of irrigation before sowing, at jointing stage and at grain filling stage, nitrogen application rate at 240 kg/hm^2 and the ratio of basal to topdressed nitrogen of 5：5, the grain yield（8 609.60 kg/hm^2）, ear number（688.2×104/hm^2） and kernel number per ear（37.9 grains） reached the highest value at W3N3R2, and the grain yield of W3N3R2 increased by 144.8% compared to the W1N0. In conclusion, in Eastern Henan where the rainfall is insufficient at the late growth stage of wheat, the irrigation-saving space in wheat production is relatively small, but the nitrogen-saving space is relatively large.展开更多
采用PCR-SSCP技术分析了小尾寒羊肌细胞生成素Myo G基因外显子2和生肌决定基因Myo D 5'侧翼区的多态性,结果表明:Myo D 5'侧翼区在小尾寒羊中检测到2种基因型(BB、AB),BB和AB基因型频率分别为0.975 0和0.025 0,A和B等位基因频...采用PCR-SSCP技术分析了小尾寒羊肌细胞生成素Myo G基因外显子2和生肌决定基因Myo D 5'侧翼区的多态性,结果表明:Myo D 5'侧翼区在小尾寒羊中检测到2种基因型(BB、AB),BB和AB基因型频率分别为0.975 0和0.025 0,A和B等位基因频率分别为0.012 5和0.987 5;小尾寒羊的Myo G外显子2不存在多态性。对Myo D 5'侧翼区的BB基因型测序分析发现,其与牛的Myo D1基因序列(XM_592330.2)相比,发生了2处突变,分别是第960位发生了T→C突变、第972位发生了C→A突变。展开更多
目的观察正常人肝细胞系LO_2和肝癌细胞系SMMC-7721中miR-1247-5p的表达,研究去甲基化药物5-杂氮-2'脱氧胞苷(5-Aza-CdR)对肝癌细胞系SMMC-7721中miR-1247-5p表达及其基因启动子区Cp G岛甲基化水平的影响。方法用不同浓度(0、5和10...目的观察正常人肝细胞系LO_2和肝癌细胞系SMMC-7721中miR-1247-5p的表达,研究去甲基化药物5-杂氮-2'脱氧胞苷(5-Aza-CdR)对肝癌细胞系SMMC-7721中miR-1247-5p表达及其基因启动子区Cp G岛甲基化水平的影响。方法用不同浓度(0、5和10μmol/L)去甲基化药物5-杂氮-2'脱氧胞苷处理SMMC-7721细胞,用甲基化特异性PCR法检测miR-1247-5p基因启动子区甲基化水平;用SYBR Green qReal Time PCR法检测miR-1247-5p的表达。结果与正常人肝细胞系LO_2相比,肝癌细胞系SMMC-7721中miR-1247-5p表达降低(P<0.05)且其基因启动子区Cp G岛甲基化水平高;经去甲基化药物干预后miR-1247-5p的表达较对照组有明显上调(P<0.01),且其基因启动子区Cp G岛甲基化水平降低。结论 miR-1247-5p基因甲基化调控可能参与了肝癌的发生。展开更多
基金Supported by the National Project of Transformation Fund for Agricultural Science and Technology Achievements:Pilot Production and Demonstration of New Wheat Variety and the Propagation of Breeder Seed(2013GB2D000300)The Special Fund of National Modern Agricultural Industry Technology System(CARS-03)~~
文摘To provide ＂more reasonable, more saving and more efficient＂ water and fertilizer application proposals, taking ‘Yujiao 5＇ as the experimental material, the effects of different irrigation times and nitrogen application treatments on the leaf area index and yield of wheat were studied using three-factor split plot method. The results showed that irrigation times, nitrogen application rate and the ratio of basa to topdressed nitrogen respectively had significant effects on the leaf area index, the yield and component factors of wheat. Under the treatment of W1（irrigation before sowing）, the leaf area index showed a positive linear correlation with nitrogen application rate; under the treatments of W2（irrigation before sowing and at jointing stage） and W3（irrigation before sowing, at jointing stage and at grain filling stages）,the leaf area index showed a positive linear correlation with nitrogen application rate at the jointing stage, booting stage and heading stage; 20 d after heading, the leaf area index showed a quadric curve relationship with nitrogen application rate at these stages, and the LAI of N3R2 was the highest. Under different irrigation times,the yield, ear number and kernels per ear showed quadric curve relationship with nitrogen application rate, 1 000-seed weight showed the trend of linear decrease with the increase of nitrogen application rate. Under the treatment combination of irrigation before sowing, at jointing stage and at grain filling stage, nitrogen application rate at 240 kg/hm^2 and the ratio of basal to topdressed nitrogen of 5：5, the grain yield（8 609.60 kg/hm^2）, ear number（688.2×104/hm^2） and kernel number per ear（37.9 grains） reached the highest value at W3N3R2, and the grain yield of W3N3R2 increased by 144.8% compared to the W1N0. In conclusion, in Eastern Henan where the rainfall is insufficient at the late growth stage of wheat, the irrigation-saving space in wheat production is relatively small, but the nitrogen-saving space is relatively large.
文摘采用PCR-SSCP技术分析了小尾寒羊肌细胞生成素Myo G基因外显子2和生肌决定基因Myo D 5'侧翼区的多态性,结果表明:Myo D 5'侧翼区在小尾寒羊中检测到2种基因型(BB、AB),BB和AB基因型频率分别为0.975 0和0.025 0,A和B等位基因频率分别为0.012 5和0.987 5;小尾寒羊的Myo G外显子2不存在多态性。对Myo D 5'侧翼区的BB基因型测序分析发现,其与牛的Myo D1基因序列(XM_592330.2)相比,发生了2处突变,分别是第960位发生了T→C突变、第972位发生了C→A突变。
文摘目的观察正常人肝细胞系LO_2和肝癌细胞系SMMC-7721中miR-1247-5p的表达,研究去甲基化药物5-杂氮-2'脱氧胞苷(5-Aza-CdR)对肝癌细胞系SMMC-7721中miR-1247-5p表达及其基因启动子区Cp G岛甲基化水平的影响。方法用不同浓度(0、5和10μmol/L)去甲基化药物5-杂氮-2'脱氧胞苷处理SMMC-7721细胞,用甲基化特异性PCR法检测miR-1247-5p基因启动子区甲基化水平;用SYBR Green qReal Time PCR法检测miR-1247-5p的表达。结果与正常人肝细胞系LO_2相比,肝癌细胞系SMMC-7721中miR-1247-5p表达降低(P<0.05)且其基因启动子区Cp G岛甲基化水平高;经去甲基化药物干预后miR-1247-5p的表达较对照组有明显上调(P<0.01),且其基因启动子区Cp G岛甲基化水平降低。结论 miR-1247-5p基因甲基化调控可能参与了肝癌的发生。
