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SEPT9基因甲基化水平在膀胱尿路上皮病变鉴别诊断中的应用
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作者 王更芳 周晓莉 +1 位作者 郭媛 饶秋 《临床与实验病理学杂志》 CAS 北大核心 2024年第4期373-379,共7页
目的探讨SEPT9基因甲基化水平在尿路上皮病变良、恶性鉴别诊断中的作用,并尝试通过联合使用SEPT9甲基化检测降低膀胱镜活检组织的假阴性率。方法对160例经病理诊断的尿路上皮病变进行SEPT9甲基化检测,分析其辅助诊断良、恶性病变的敏感... 目的探讨SEPT9基因甲基化水平在尿路上皮病变良、恶性鉴别诊断中的作用,并尝试通过联合使用SEPT9甲基化检测降低膀胱镜活检组织的假阴性率。方法对160例经病理诊断的尿路上皮病变进行SEPT9甲基化检测,分析其辅助诊断良、恶性病变的敏感性和特异性;比较癌和癌旁组织间SEPT9基因甲基化水平;在12例首次膀胱镜活检为炎症或良性病变而后续活检、电切为癌的病理组织中分别检测SEPT9基因甲基化水平。结果SEPT9基因甲基化水平鉴别诊断尿路上皮良、恶性病变的敏感性、特异性分别为98%(88/90)和94%(66/70)。47%(7/15)的癌旁组织中SEPT9基因甲基化检测结果为阳性,显示癌旁组织表观遗传修饰异常;12例首次膀胱镜活检为炎症或良性病变的组织中SEPT9基因甲基化检测阳性率为67%(8/12),再次活检、电切病理证实为癌的组织SEPT9甲基化检测阳性率为100%(12/12)。结论SEPT9基因甲基化检测可以辅助尿路上皮病变良、恶性鉴别诊断,并可能有助于减少膀胱镜活检组织的假阴性率。 展开更多
关键词 尿路上皮病变 活检组织 鉴别诊断 sept9甲基化
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SEPT12基因突变导致弱畸精子症引起男性不育
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作者 郭森朝 于慧 +6 位作者 顾梦 吴宝燕 李阔阔 汤冬冬 贺小进 曹云霞 吕明荣 《安徽医科大学学报》 CAS 北大核心 2024年第6期939-946,共8页
目的研究septins基因家族成员SEPT12在人类精子发生过程的作用及其对精子运动力和精子超微结构的影响。方法对收集的375例弱畸精子症患者外周血提取DNA进行全外显子测序(WES),筛选出一例携带SEPT12复合杂合突变的特发性不育患者,行Sange... 目的研究septins基因家族成员SEPT12在人类精子发生过程的作用及其对精子运动力和精子超微结构的影响。方法对收集的375例弱畸精子症患者外周血提取DNA进行全外显子测序(WES),筛选出一例携带SEPT12复合杂合突变的特发性不育患者,行Sanger测序验证,家系共分离分析。用苏木精-伊红染色(HE)及扫描电镜(SEM)分析患者精子形态畸形,透射电镜(TEM)分析精子超微结构缺陷。然后,通过Western blot及免疫荧光(IF)分析突变对其蛋白水平及位置的影响及缺陷结构标志物位置及水平的变化情况。结果在1位弱畸精子症患者中筛选并鉴定SEPT12基因复合杂合突变c.C332A(p.T111K)和c.406_416 del TGCTCGTATTG(p.q 136 VFS*39)。Sanger测序验证,符合家系共分离遗传模式。突变导致其蛋白表达缺失、精子活力降低和精子形态畸形,主要包括短尾、卷尾、精子头部不规则。精子超微结构显示精子鞭毛中段与主段连接处的环缺失、精子头部顶体膜脱落、核空泡。精子鞭毛中段线粒体鞘排列紊乱、中央二联管缺失、微管双联体缺失及部分放射轴辐缺失。Western blot及IF结果显示SEPT家族相关蛋白SEPT4蛋白水平降低,SEPT6蛋白不变,顶体相关蛋白ACTL7A和ACROSIN蛋白缺失,线粒体及轴丝相关蛋白TOMM20,SPAG6和RSPH3蛋白水平显著降低。结论SEPT12基因突变引起SEPT12蛋白缺失,导致精子鞭毛中段与主段连接处环缺失,引起精子顶体、线粒体鞘及鞭毛组装异常。 展开更多
关键词 男性不育 弱畸精子症 sept12 全外显子测序
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超声微泡介导miR-545-3p通过调节SEPT2抑制乳腺癌的恶性进展
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作者 李雯静 张强 《现代肿瘤医学》 CAS 2024年第7期1222-1228,共7页
目的:研究超声微泡介导miR-545-3p通过调节胞裂蛋白2(septin 2,SEPT2)抑制乳腺癌恶性进展的机制。方法:以实时荧光定量PCR和免疫印迹实验检测人正常乳腺上皮细胞MCF-10A、人乳腺癌细胞MCF-7、HS-578T及HCC-1937细胞中miR-545-3p、SEPT2... 目的:研究超声微泡介导miR-545-3p通过调节胞裂蛋白2(septin 2,SEPT2)抑制乳腺癌恶性进展的机制。方法:以实时荧光定量PCR和免疫印迹实验检测人正常乳腺上皮细胞MCF-10A、人乳腺癌细胞MCF-7、HS-578T及HCC-1937细胞中miR-545-3p、SEPT2表达。体外培养MCF-7细胞并随机分为对照组、空微泡组、miR-545-3p微泡组、miR-545-3p+SEPT2过表达微泡组,分组处理后以实时荧光定量PCR和免疫印迹实验检测各组细胞miR-545-3p、SEPT2表达;以CCK-8、集落形成实验与流式细胞实验检测各组细胞增殖与凋亡;以细胞划痕、Transwell侵袭实验检测各组细胞迁移与侵袭。于裸鼠腹壁皮下接种MCF-7细胞构建其移植瘤模型并以同样方法分组,经分组处理后检测各组裸鼠肿瘤体积与质量。结果:与MCF-10A细胞相比,MCF-7、HS-578T及HCC-1937细胞中miR-545-3p表达降低(P<0.05),SEPT2 mRNA与蛋白表达升高(P<0.05)。与对照组相比,miR-545-3p微泡组细胞miR-545-3p表达、凋亡率升高(P<0.05),细胞SEPT2 mRNA与蛋白表达、细胞活力、集落形成率、迁移率、侵袭数、裸鼠肿瘤体积与质量降低(P<0.05);与miR-545-3p微泡组相比,miR-545-3p+SEPT2过表达微泡组细胞凋亡率降低(P<0.05),细胞SEPT2 mRNA与蛋白表达、细胞活力、集落形成率、迁移率、侵袭数、裸鼠肿瘤体积与质量升高(P<0.05)。结论:超声微泡介导miR-545-3p可通过降低SEPT2表达而减弱乳腺癌细胞增殖、迁移与侵袭,促使其凋亡,并抑制裸鼠移植瘤生长,最终抑制乳腺癌细胞恶性进展。 展开更多
关键词 超声微泡 miR-545-3p sept2 乳腺癌 恶性进展
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Analysis of the Influence of Clothing on Characterization from Symbolism: Taking The Scarlet Letter, Daisy Miller, and Dry September as Examples
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作者 HU Ya-ling 《Journal of Literature and Art Studies》 2023年第12期978-982,共5页
In the novels, the clothing worn by the characters are symbols made and used by the authors to express the characters’ personality and deepen the theme, and grasping their symbolism helps to enter into the interior o... In the novels, the clothing worn by the characters are symbols made and used by the authors to express the characters’ personality and deepen the theme, and grasping their symbolism helps to enter into the interior of the novels and understand their essence. This paper will analyze and interpret the clothing of typical female characters in The Scarlet Letter, Daisy Miller and Dry September from the perspective of symbolism, so as to illustrate how the symbolism of clothing, as a kind of symbols, shapes the image of the main characters. 展开更多
关键词 SYMBOLISM CLOTHING CHARACTERIZATION The Scarlet letter Daisy Miller Dry september
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Letters and Visits System Optimization and Implementation
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作者 Mu Tao Xi Chen Dan Qian 《Journal of Software Engineering and Applications》 2024年第9期731-739,共9页
The letters and visits system plays a vital role in government work, serving as a crucial tool for supervising law enforcement and administrative conduct, ensuring public officials’ integrity, and promoting governanc... The letters and visits system plays a vital role in government work, serving as a crucial tool for supervising law enforcement and administrative conduct, ensuring public officials’ integrity, and promoting governance by law. As Chinese citizens’ political awareness grows, the volume of letters and visits has increased steadily. This paper reviews the current state of letters and visits information construction, identifies challenges and problems in system integration, presents integration ideas for existing systems, and proposes an innovative approach to letters and visits system integration. This research aims to provide valuable insights and guidance for other units undertaking similar system integration efforts. 展开更多
关键词 letters and Visits INFORMATIZATION System Integration
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Cheyne-Stokes Respiration in Heart Failure Is Not a Therapeutic Target: A Lesson from CANPAP, SERVE-HF and ADVENT-HF Trials (Letter to the Editor)
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作者 Fumihiko Yasuma Junichiro Hayano 《Health》 2024年第6期561-564,共4页
Letter to the Editor.
关键词 letter to the Editor
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Letter to the Editor: Reverse T3;a Reliable Diagnostic Test for Discerning between Euthyroid Sick and Central Hypothyroidism
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作者 Udaya M. Kabadi Sarah Exley 《Open Journal of Endocrine and Metabolic Diseases》 2024年第2期73-74,共2页
关键词 letter ISM Sick
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A Letter to Mr.Tan Kah Kee
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作者 颜宇轩 叶彩霞(指导) 《疯狂英语(初中天地)》 2024年第10期66-68,共3页
Dear Mr.Tan Kah Kee,I am a student from Southern Fujian in the 21st century.And I am writing this letter to express my deep admiration for your patriotism and outstanding contribution.You are a true patriot.
关键词 And letter letter
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A Letter to My Pen Pal Mike
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作者 沈一凡 张超(指导) 《中学生英语》 2024年第5期7-7,共1页
Dear Mike,I am a middle school student this new term.I love my new school.It is big and clean.There are many green trees in it.And there is a big garden in front of the playground,too.Our classroom is big,too.I have 4... Dear Mike,I am a middle school student this new term.I love my new school.It is big and clean.There are many green trees in it.And there is a big garden in front of the playground,too.Our classroom is big,too.I have 45 classmates.They are all very nice.We have some good teachers,too.Our English teacher is Mr.Zhang. 展开更多
关键词 And letter CLASSROOM
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A Letter to Qian Xuesen
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作者 温欣澎 闫明(指导) 《疯狂英语(初中天地)》 2024年第10期69-69,共1页
Dear Grandpa Qian,As a middle school student,I am deeply touched by your patriotism(爱国精神)and dedication(奉献精神).In 1949,when the People’s Republic of China was founded and everything remained to be developed.Yo... Dear Grandpa Qian,As a middle school student,I am deeply touched by your patriotism(爱国精神)and dedication(奉献精神).In 1949,when the People’s Republic of China was founded and everything remained to be developed.You gave up a well-paid job in the United States and returned to China at the risk of your life. 展开更多
关键词 letter GRAND ISM
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SEPT12基因新突变致弱畸精子症男性不育1例
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作者 刘红 周永红 +2 位作者 成艳君 杨宇霞 李雪梅 《汕头大学医学院学报》 2023年第2期120-121,共2页
1病例资料患者男,31岁,夫妻同居3年,性生活规律,每月5~7次,同房时阴茎勃起正常,阴道内射精。配偶平素月经规则,周期约28 d。经期7d,经量正常,各项基础内分泌检测正常。近3年未采取避孕措施而未育,于2018年5月到南方医科大学附属深圳市... 1病例资料患者男,31岁,夫妻同居3年,性生活规律,每月5~7次,同房时阴茎勃起正常,阴道内射精。配偶平素月经规则,周期约28 d。经期7d,经量正常,各项基础内分泌检测正常。近3年未采取避孕措施而未育,于2018年5月到南方医科大学附属深圳市妇幼保健院就诊。生殖系统检查显示,患者生殖器外形和功能正常,左、右睾丸体积均约为12 mL,质地正常。 展开更多
关键词 sept12基因 弱畸精子症 男性不育 精子环
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CircSEPT9调节miR-650/HSPB1轴对胶质瘤细胞恶性生物学行为影响的探讨 被引量:1
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作者 任成波 赵丽霞 +5 位作者 杜晓猛 李锦秋 邹念东 王聪 宋晓 张志林 《中国临床新医学》 2023年第8期842-849,共8页
目的探讨环状RNA(CircRNA)SEPT9调节miR-650/热休克蛋白B1(HSPB1)轴对胶质瘤细胞恶性生物学行为的影响。方法通过实时定量聚合酶链式反应(qRT-PCR)、Western blot法检测胶质瘤组织、癌旁组织、人类正常神经胶质细胞HEB及胶质瘤细胞系LN1... 目的探讨环状RNA(CircRNA)SEPT9调节miR-650/热休克蛋白B1(HSPB1)轴对胶质瘤细胞恶性生物学行为的影响。方法通过实时定量聚合酶链式反应(qRT-PCR)、Western blot法检测胶质瘤组织、癌旁组织、人类正常神经胶质细胞HEB及胶质瘤细胞系LN18、SW1088、U251中CircSEPT9、miR-650、HSPB1蛋白表达水平。将U251细胞分为对照组(NC组)、si-NC组、si-CircSEPT9组、mimic NC组、miR-650 mimic组、si-CircSEPT9+inhibitor NC组、si-CircSEPT9+miR-650 inhibitor组、si-CircSEPT9+pcDNA组、si-CircSEPT9+pcDNA-HSPB1组。采用qRT-PCR法检测CircSEPT9、miR-650表达水平。采用Western blot法检测HSPB1、细胞周期蛋白D1(Cyclin D1)、Caspase-3、基质金属蛋白酶(MMP)-2、MMP-9蛋白表达水平。通过双荧光素酶基因实验验证CircSEPT9与miR-650、miR-650与HSPB1的关系。结果在胶质瘤组织和细胞中,CircSEPT9和HSPB1蛋白呈高表达,miR-650呈低表达,且在U251细胞中CircSEPT9以及HSPB1蛋白表达水平最高,miR-650表达水平最低,差异有统计学意义(P<0.05)。沉默CircSEPT9或过表达miR-650均可抑制U251细胞增殖、侵袭、迁移及Cyclin D1、MMP-2、MMP-9蛋白表达水平,促进细胞凋亡及Caspase-3蛋白表达。下调miR-650或过表达HSPB1均可减弱沉默CircSEPT9对U251细胞的作用影响。CircSEPT9与miR-650、miR-650与HSPB1存在靶向关系。结论沉默CircSEPT9可能通过海绵化上调miR-650来抑制HSPB1表达,进而抑制U251细胞增殖、迁移与侵袭,促进细胞凋亡。 展开更多
关键词 环状RNA sept9 miR-650 热休克蛋白B1 胶质瘤 生物学行为
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CA125、HE4和mSEPT9对子宫内膜癌临床诊断价值的研究 被引量:6
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作者 吴健亮 闫洪超 《徐州医科大学学报》 CAS 2023年第1期54-58,共5页
目的评估血清中人附睾蛋白4(HE4)、糖类抗原125(CA125)、尿液中SEPT9基因甲基化(mSEPT9)水平在诊断子宫内膜癌的临床价值。方法选取2021年11月—2022年5月于徐州医科大学附属医院确诊为子宫内膜癌的患者40例作为研究组。选取同期子宫内... 目的评估血清中人附睾蛋白4(HE4)、糖类抗原125(CA125)、尿液中SEPT9基因甲基化(mSEPT9)水平在诊断子宫内膜癌的临床价值。方法选取2021年11月—2022年5月于徐州医科大学附属医院确诊为子宫内膜癌的患者40例作为研究组。选取同期子宫内膜良性病变患者39例作为对照组。采用化学发光法检测2组患者血清CA125、HE4水平。采用实时荧光定量PCR法检测2组患者尿液mSEPT9 Ct值。结果研究组血清CA125、HE4水平显著高于对照组(P<0.05)。根据ROC曲线,将血清CA125、HE4和尿液mSEPT9 Ct值的截断值分别设为14.65 kU/L,52.44 pmol/L,38.95。研究组CA125、HE4、mSEPT9阳性率显著高于对照组,差异有统计学意义(P<0.05)。CA125、HE4、mSEPT9的曲线下面积均>0.5,具有临床诊断意义,mSEPT9单项检测特异度明显高于HE4及CA125。CA125水平与病理分期、病理分级、淋巴脉管转移相关(P<0.05);HE4水平与年龄、肌层浸润深度相关(P<0.05);mSEPT9阳性率与手术病理分期、肌层浸润深度、淋巴及脉管转移相关(P<0.05)。结论尿液mSEPT9检测在子宫内膜癌筛查中具有较大潜力,为子宫内膜癌筛查提供新的思路。 展开更多
关键词 子宫内膜癌 sept 9甲基化 肿瘤标志物 联合检测
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SEPT9甲基化检测在结直肠癌早期诊断中价值的研究进展 被引量:2
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作者 董优优(综述) 陈昌国(审校) 《国际检验医学杂志》 CAS 2023年第20期2548-2553,共6页
结直肠癌是我国常见恶性消化道肿瘤之一,其发病率逐年上升且预后不好,病死率较高。早发现、早诊断可有效降低结直肠癌致死率。目前,临床用于结直肠癌诊断及监测的肿瘤标志物有癌胚抗原(CEA)、糖类抗原19-9(CA199)、糖类抗原242(CA242)等... 结直肠癌是我国常见恶性消化道肿瘤之一,其发病率逐年上升且预后不好,病死率较高。早发现、早诊断可有效降低结直肠癌致死率。目前,临床用于结直肠癌诊断及监测的肿瘤标志物有癌胚抗原(CEA)、糖类抗原19-9(CA199)、糖类抗原242(CA242)等,这些标志物在结直肠癌早期诊断中的临床价值不够理想。因此,探索新的、早期诊断价值更高的肿瘤标志物用于结直肠癌筛查是当前研究的热点之一。SEPT9是一种抑癌基因,SEPT9基因甲基化检测对结直肠癌早期诊断有较高的特异度和灵敏度,该文就SEPT9基因甲基化检测在结直肠癌早期诊断中的研究进展进行综述。 展开更多
关键词 结直肠癌 sept9 甲基化 肿瘤标志物 早期诊断
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Letter from Ms.Cordula Gabel-Obermaier Executive for IOFF-Subspecialty Fellowships (IOFF-IJO Subspecialty Fellowship for Dr.Pinar Kosekahya)
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《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第3期F0003-F0003,共1页
DearProf.Xiu-Wen Hu,I hope this find you well.My sincere best wishes for a happy and peaceful new year!Dr.Pinar Kosekahya from Turkey was the lucky recipient of the IJO 2022 funds thank you so much for the generous co... DearProf.Xiu-Wen Hu,I hope this find you well.My sincere best wishes for a happy and peaceful new year!Dr.Pinar Kosekahya from Turkey was the lucky recipient of the IJO 2022 funds thank you so much for the generous contribution. 展开更多
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Appreciation letter from Dr.Pinar Kosekahya from Turkey the Icuky recipient of the IJO 2022 funds
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作者 Xiu-Wen Hu 《国际眼科杂志》 CAS 北大核心 2023年第4期F0003-F0003,共1页
I am Dr.Pinar Kosekahya from Ankara,Turkey.I wanted to personally thank you and the organization you represent,for sponsoring me in an IOFF-Subspeciality Fellowship.I am very grateful for this opportunity and I want y... I am Dr.Pinar Kosekahya from Ankara,Turkey.I wanted to personally thank you and the organization you represent,for sponsoring me in an IOFF-Subspeciality Fellowship.I am very grateful for this opportunity and I want you to know that I will not take it for granted. 展开更多
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Appreciation letter from Dr.Pinar Kosekahya from Turkey the lcuky recipient of the IJO 2022 funds
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作者 Pinar Kosekahya 《国际眼科杂志》 CAS 北大核心 2023年第3期F0003-F0003,共1页
To Dear Prof.Xiu-Wen Hu International Journal of Ophthalmology Press,China I am Dr.Pinar Kosekahya from Ankara,Turkey.I wanted to personally thank you and the organization you represent,for sponsoring me in an IOFF-Su... To Dear Prof.Xiu-Wen Hu International Journal of Ophthalmology Press,China I am Dr.Pinar Kosekahya from Ankara,Turkey.I wanted to personally thank you and the organization you represent,for sponsoring me in an IOFF-Subspeciality Fellowship.I am very grateful for this opportunity and I want you to know that I will not take it for granted.Once I was accepted into the International Ophthalmology Fellowship Foundation,I became overwhelmed with excitement.My country was in an economic crisis due to Covid-19 pandemic and I was very worried that I would miss this chance if I can not get a sponsorship.I was extremely happy when dear Cordula informed me about my Educational Grant. 展开更多
关键词 letter thank TURKEY
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Appreciation letter from Dr.Pinar Kosekahya from Turkey the lcuky recipient of the IJO 2022 funds
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《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第4期F0002-F0002,共1页
To Dear Prof.Xiu-Wen Hu International Journal of Ophthalmology Press,China I am Dr.Pinar Kosekahya from Ankara,Turkey.I wanted to personally thank you and the organization you represent,for sponsoring me in an IOFF-Su... To Dear Prof.Xiu-Wen Hu International Journal of Ophthalmology Press,China I am Dr.Pinar Kosekahya from Ankara,Turkey.I wanted to personally thank you and the organization you represent,for sponsoring me in an IOFF-Subspeciality Fellowship.I am very grateful for this opportunity and I want you to know that I will not take it for granted. 展开更多
关键词 letter TURKEY thank
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Appreciation letter from Dr.Pinar Kosekahya from Turkey the lcuky recipient of the IJO 2022 funds
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作者 Xiu-Wen Hu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第2期F0002-F0002,共1页
To Dear Prof.Xiu-Wen Hu International Journal of Ophthalmology Press,China I am Dr.Pinar Kosehahya Bran Ankara,Turkey.I wanted to personally thank you and the organization you represent,for sponsoring me in an IOFF-Su... To Dear Prof.Xiu-Wen Hu International Journal of Ophthalmology Press,China I am Dr.Pinar Kosehahya Bran Ankara,Turkey.I wanted to personally thank you and the organization you represent,for sponsoring me in an IOFF-Subspeciality Fellowship.I am very grateful for this opportunity and I want you to know that I will not take it for granted.Once was accepted into the Intetrnational Ophthalmology Fellowship Foundation,I became overwhelmed with excitement.My country was in an economie crisis due to Covid-19 pandemic and I was ver worried that I would miss this chance if I can not get a sponsorship.I was extremely happy when dear Cordula informed me about my Educational Grant. 展开更多
关键词 letter thank TURKEY
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Appreciation letter from Dr. Pinar Kosekahya from Turkey the lcuky recipient of the IJO 2022 funds
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作者 Pinar Kosekahya 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第3期F0002-F0002,共1页
ToDearProf.Xiu-Wen Hu International Journal of Ophthalmology Press,China I am Dr.Pinar Kosekahya from Ankara,Turkey.I wanted to personally thank you and the organization you represent,for sponsoring me in an IOFF-Subs... ToDearProf.Xiu-Wen Hu International Journal of Ophthalmology Press,China I am Dr.Pinar Kosekahya from Ankara,Turkey.I wanted to personally thank you and the organization you represent,for sponsoring me in an IOFF-Subspeciality Fellowship.I am very grateful for this opportunity and I want you to know that I will not take it for granted.Once I was accepted into the International Ophthalmology Fellowship Foundation,I became overwhelmed with excitement.My country was in an economic crisis due to Covid-19 pandemic and I was very worried that I would miss this chance if I can not get a sponsorship.I was extremely happy when dear Cordula informed me about my Educational Grant. 展开更多
关键词 letter TURKEY thank
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