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及物性、语篇类型与立场功能的关联——从“一再”与“再三”的情感差异谈起
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作者 孙嘉铭 《汉语学报》 CSSCI 北大核心 2024年第4期68-79,共12页
“一再”与“再三”是现代汉语中的一对近义词,两者的意义接近,但在感情色彩方面存在明显差异。本文指出“一再”与“再三”的句法语义差别可以从“及物性”角度获得统一解释,其中“一再”所在小句具有低及物性,“再三”所在小句具有高... “一再”与“再三”是现代汉语中的一对近义词,两者的意义接近,但在感情色彩方面存在明显差异。本文指出“一再”与“再三”的句法语义差别可以从“及物性”角度获得统一解释,其中“一再”所在小句具有低及物性,“再三”所在小句具有高及物性。两者的及物性等级不同,会带来明显不同的语用效应,并衍生出不同的立场表达功能。其中,复句关系所构成的话语环境差异是形成“一再”与“再三”立场功能对立的直接诱因。“一再”优势分布的转折复句与反预期具有天然关联,而反预期又与负面立场密切相关。本文的研究表明,负面立场的规约化动因可以追溯至词语的句法语义特征,语义与语用在负面立场的规约化过程中存在密切互动关联。 展开更多
关键词 “一再” “再三” 及物性 语篇类型 立场功能
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“一再”和“再三”的辨析 被引量:11
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作者 周小兵 邓小宁 《汉语学习》 北大核心 2002年第1期61-64,共4页
关键词 中国 汉语 “一再” “再三” 词语 词义
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“一再”和“再三”辨析 被引量:1
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作者 张梅 《和田师范专科学校学报》 2006年第5期240-240,共1页
本文从“一再”和“再三”对动词的要求,对时间的要求,对几种特殊格式的要求几方面,较为细致地辨析了二者的区别,以便帮助民族学生在学习汉语的过程中更好地掌握和使用。
关键词 辨析 民族学生 汉语 “一再” “再三”
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“一再”和“再三”的句法和语义分析 被引量:3
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作者 陆苏静 《现代语文(下旬.语言研究)》 2008年第8期75-77,共3页
“一再”和“再三”两词的语义和用法接近。在句法上,二词存在一些较为显著的差异;在语义上,二词有细微的不同之处。“一再”表示的“一次一次”可以有较长的时间跨度,动作次数之间也可以有较长的时间间隔,而“再三”表示的“一次... “一再”和“再三”两词的语义和用法接近。在句法上,二词存在一些较为显著的差异;在语义上,二词有细微的不同之处。“一再”表示的“一次一次”可以有较长的时间跨度,动作次数之间也可以有较长的时间间隔,而“再三”表示的“一次一次”重在短时间内动作次数的反复。“再三”有表集中量的“多次”义,“一再”没有。 展开更多
关键词 “一再” “再三” “频率副词” 句法差异 语用、语义差异
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“一再”和“再三”的辨析
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作者 王欢 《商情》 2013年第27期94-94,共1页
“一再”和“再三”是现代汉语中的两个副词,二者在使用的过程中,有时可以互换,但在用法上还是有各自的使用范围和特点,并不完全对等。在对外汉语教学中,我们需要对这两个词加以区分,否则就有可能使留学生对二者产生使用上的偏误... “一再”和“再三”是现代汉语中的两个副词,二者在使用的过程中,有时可以互换,但在用法上还是有各自的使用范围和特点,并不完全对等。在对外汉语教学中,我们需要对这两个词加以区分,否则就有可能使留学生对二者产生使用上的偏误。本文试从词汇、句法和语义平面对“一再”和“再三”进行简要考察和辨析。 展开更多
关键词 “一再”“再三”词汇句法语义
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“一A再A”的构式化 被引量:1
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作者 陈勇 余梓瀚 《新疆大学学报(哲学社会科学版)》 CSSCI 2023年第5期143-149,共7页
“一……,再……”在先秦时期十分常见,最初为松散的涉数序列表达形式,明清时期逐渐演变为内部紧凑凝固的构式“一A再A”,表“动作的反复;程度递增”。在历时演变过程中,“省缩”是其构式化的主要诱因,另外还有:构成成分的分裂;变项A的... “一……,再……”在先秦时期十分常见,最初为松散的涉数序列表达形式,明清时期逐渐演变为内部紧凑凝固的构式“一A再A”,表“动作的反复;程度递增”。在历时演变过程中,“省缩”是其构式化的主要诱因,另外还有:构成成分的分裂;变项A的泛化、谓词化及单音化;语境影响,如“对举”形成的语境效应、语境吸收。该构式的构式化机制主要有重新分析、隐喻、韵律节奏、表述的程度化等。 展开更多
关键词 一A再A 构式化 诱因 机制
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“再一个”:从数量到指代再到连接的语义演变——兼论数量范畴的双重演化路径 被引量:1
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作者 吕佩 《新疆大学学报(哲学社会科学版)》 CSSCI 2023年第1期145-150,共6页
文章以“再一个”为例,考察一则在现当代汉语中发生并完成的语义演变现象:从数量范畴到指代范畴再到连接范畴。文章认为,“再一个”从数量范畴演变为连接范畴,中间经过指代范畴。随着语义演变的完成,“再一个”从短语词汇化为一个三音... 文章以“再一个”为例,考察一则在现当代汉语中发生并完成的语义演变现象:从数量范畴到指代范畴再到连接范畴。文章认为,“再一个”从数量范畴演变为连接范畴,中间经过指代范畴。随着语义演变的完成,“再一个”从短语词汇化为一个三音节连词。“再一个”与“再说”的差异主要体现在三个方面:语体、词汇化程度和主观性。汉语中的数量范畴存在双重演化路径:关联化和情态化。 展开更多
关键词 “再一个” 语义演变 关联化 情态化
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“一A再A”构式研究
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作者 陈勇 《临沂大学学报》 2020年第6期100-108,共9页
“一A再A”在汉语中使用频率相当高,基于汉语语料库,文章对该构式作了全面考察,发现:形式特征上,该构式凝固性强,且A的音节主要为单音节,词性通常为动词,少数也可是形容词、名词、量词等;句法功能方面,该构式在句中主要充当谓语、状语... “一A再A”在汉语中使用频率相当高,基于汉语语料库,文章对该构式作了全面考察,发现:形式特征上,该构式凝固性强,且A的音节主要为单音节,词性通常为动词,少数也可是形容词、名词、量词等;句法功能方面,该构式在句中主要充当谓语、状语、定语、补语等;其构式义也较丰富,如“持续或反复”“妥协或退让”“斥责或抱怨”“性状程度的加深”;语用方面,该构式具有一定的贬义倾向。此外,与相仿的构式“一再A”对比,发现它们之间存在较大的差异。 展开更多
关键词 一A再A 结构形式 句法 构式义 语用
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Gender differences in hepatic ischemic reperfusion injury in rats are associated with endothelial cell nitric oxide synthase-derived nitric oxide 被引量:6
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作者 PingLu FangLiu +5 位作者 Chun-YouWang Dao-DaChen ZhongYao YuanTian Jing-HuiZhang Yi-HuaWu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第22期3441-3445,共5页
AIM: This study was designed to examine the hypothesis that gender differences in I/R injury are associated withendothelial cell nitric oxide synthase (eNOS)-derived nitric oxide (NO).METHODS: Wistar rats were randomi... AIM: This study was designed to examine the hypothesis that gender differences in I/R injury are associated withendothelial cell nitric oxide synthase (eNOS)-derived nitric oxide (NO).METHODS: Wistar rats were randomized into seven experimental groups (12 animals per group). Except for the sham operated groups, all rats were subjected to total liver ischemia for 40 min followed by reperfusion. All experimental groups received different treatments 45 min before the laparotomy. For each group, half of the animals (six) were used to investigate the survival; blood samples and liver tissues were obtained in the remaining six animals after 3 h of reperfusion to assess serum NO, alanine aminotransferase (ALT) and TNF-α levels, liver tissuemalondialdehyde (MDA) content, and severity of hepatic I/R injury.RESULTS: Basal serum NO levels in female sham operated (FS) group were nearly 1.5-fold of male sham operated (MS) group (66.7±11.0 μmol/L vs 45.3±10.1μmol/L, P<0.01). Although serum NO levels decreased significantly after hepatic I/R (P<0.01, vs sham operated groups), they were still significantly higher in female rat (F) group than in male rat (M) group (47.8±8.6 μmol/L vs 23.8±4.7 μmol/L, P<0.01). Serum ALT and TNF-α levels, and liver tissue MDA content were significantly lower in F group than in M group (370.5±46.4 U/L, 0.99±0.11 μg/L and 0.57±0.10 μmol/g vs668.7±78.7 U/L, 1.71±0.18 μg/Land 0.86±0.11 μmol/g, respectively, P<0.01). I/R induced significant injury to the liver both in M and F groups (P<0.01 vs sham operated groups). But the degree of hepatocyte injury was significantly milder in F group than in M group (P<0.05 and P<0.01). The median survival time was six days in F group and one day in M group. The overall survival rate was significantly higher in F group than in M group (P<0.05). When compared with male rats pretreated with saline (M group), pretreatment of male rats with 17-β- estradiol (E2) (M+E2 group) significantly increased serum NO levels and significantly decreased serum ALT and TNF-α levels, and liver tissue MDA content after I/R (P<0.01).The degree of hepatocyte injury was significantly decreased and the overall survival rate was significantly improved in M+E2 group than in M group (P<0.01 and P<0.05). TheNOS inhibitor Nw-nitro-L-arginine methyl ester (L-NAME) treatment could completely abolish the protective effects of estrogen in both male and female rats. CONCLUSION: The protective effects afforded to female rats subjected to hepatic I/R are associated with eNOSderived NO. 展开更多
关键词 Gender identity LIVER Reperfusion injury Endothelial constitutive nitric oxide synthase
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Experimental Research and Mathematical Modelling for Coal Reburning in Furnace
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作者 XIANG Jun SU Sheng +3 位作者 SUN Lu-shi SUN Xue-xi ZHANG Zhong-xiao ZHU Ji-mu 《Journal of China University of Mining and Technology》 EI 2006年第3期301-307,共7页
Reburning technology is one of the most cost-effective NOx reduction strategies for coal combustion systems. In this paper, a nitric oxide submodel incorporated into a comprehensive coal combustion model was developed... Reburning technology is one of the most cost-effective NOx reduction strategies for coal combustion systems. In this paper, a nitric oxide submodel incorporated into a comprehensive coal combustion model was developed for predicting NOx reduction in a 93 kW laboratory-scale coal combustion furnace by reburning. This NO submodel, including reburning mechanism, requires the solution of only two transport equations to model the behavior of NO reduction in the reburning process. A number of experiments have been performed in the same furnace, and the experimental data obtained from the optimized reburn configuration was used to validate the model. Measurements and predictions both show above 50% reduction of NO emissions for the optimized reburning process. Profile comparisons show that the predicted temperature and oxygen concentration match well with the measurements, and the general trend of predicted NO concentration is very similar to that measured. The results of this study show that the present nitric oxide submodel depicts quite well the observed behaviour of NO annihilation in the reburning process. It is expected that this usable and computationally economic model represents a useful tool to simulate the gaseous fuel reburning process for the researchers concerned with practical combustors. 展开更多
关键词 coal combustion nitric oxide REBURNING experimental research mathematical modeling
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Urbanization-related warming in local temperature records:a review 被引量:7
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作者 WANG Jun YAN Zhong-Wei 《Atmospheric and Oceanic Science Letters》 CSCD 2016年第2期129-138,共10页
There have long been arguments about the impact of urbanization on local meteorological observations. This letter reviews up-to-date studies of the urbanization-related warming in the observed land surface air tempera... There have long been arguments about the impact of urbanization on local meteorological observations. This letter reviews up-to-date studies of the urbanization-related warming in the observed land surface air temperature series in China. Many previous studies have suggested that, over the past few decades, the local warming due to urbanization could have been about 0.1 °C/10 yr, or even larger. However, based on recently developed homogenized temperature records, the estimated urban bias is smaller. Major uncertainties arise from either the data quality or the techniques used to estimate the urbanization effect. A key example is the ‘observationminus-reanalysis' method, which tends to overestimate the urban signal in this region, partly due to systematic bias in the multi-decadal variability of surface air temperature in the reanalysis data. It is expected that improved numerical modeling with high-resolution information regarding the changing land surface in the region will help to further understand and quantify the effect of urbanization in local temperature records. 展开更多
关键词 URBANIZATION China land surface air temperature HOMOGENIZATION observation-minus-reanalysis method uncertainty
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Northward Movement and Highly-Qualified Cultivation Technology of Super-early Maturing Ratoon Rice
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作者 周晓东 《Agricultural Science & Technology》 CAS 2015年第4期716-718,768,共4页
Objective] The alm was to expIore northward movement and highIy-quali-fied cuItivation technoIogy of super-earIy maturing ratoon rice. [Method] The 2nd-sea-son rice was cuItivated in the test with suitabIe temperature... Objective] The alm was to expIore northward movement and highIy-quali-fied cuItivation technoIogy of super-earIy maturing ratoon rice. [Method] The 2nd-sea-son rice was cuItivated in the test with suitabIe temperature, heat water and fertiIiz-er, on basis of stubbIes of the 1st-season rice. Hence, rice can be harvested twice per year, with short growth periods, and high yields, and the cuItivation is simpIe to be operated, avoiding the peak of disease damages, so that pesticides are not re-quired. [Result] The experiment of northward movement of super-earIy rice achieved success in Shenyang on October 9, 2014, with super-earIy maturing japonica rice 8501. [Conclusion] Northward movement of ratoon rice enjoys a promising prospect in increasing crop yield and guaranteeing food security. 展开更多
关键词 Retoon rice Northward movement Growing in one season and harvest-ing in two seasons High-yleIding cuItivatlon PROSPECT
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Numerical Modeling and Investigation of PEM Fuel Cell for Performance Augmentation
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作者 Abdullah A. Alshorman 《Journal of Energy and Power Engineering》 2014年第5期857-869,共13页
Fuel cell is an important promised source of clean renewable energy that is being under extensive scientific investigation and developments. One important type of fuel cells is PEM (proton exchange membrane fuel cell... Fuel cell is an important promised source of clean renewable energy that is being under extensive scientific investigation and developments. One important type of fuel cells is PEM (proton exchange membrane fuel cell), which is considered in this study. Specifically, this study aimed at building-up of mathematical computerized model to simulate the stages of PEM fuel cell and to investigate the effects of cell design and operation parameters on its general performance. These include membrane thickness, cell area, hydrogen pressure and ionic current density. One-dimensional model has been introduced and appealed to analyze the effects of PEM fuel cell parameters on its overall performance. The results demonstrate that the cell power (and electrical efficiency) reduces as the thickness of cell membrane gets larger. Moreover, the peak point of cell power gets its maximum value at membrane thickness of 0.005 cm and its minimum value at 0.05 cm. However, the optimum value for ionic current density to get relative high cell power and electrical efficiency is equal 0.81 A/cm^2. These findings enhance research efforts toward new design and materials of PEM fuel cell. 展开更多
关键词 Fuel cell MEMBRANE modeling thickness cell power current density.
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Protective effect of nitric oxide on hepatopulmonary syndrome from ischemia-reperfusion injury 被引量:7
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作者 Tong-Jin Diao, Xin Chen +7 位作者 Li-Hua Deng, Han-Xiang ChenI Yan Liang Xiao-Dong Zhao Qing-Hua Wang, Wei-Sheng Yuan Bai-Chun Gao Yong Ye 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第25期3310-3316,共7页
AIM: To evaluate immunological protection of nitric ox- ide (NO) in hepatopulmonary syndrome and probable mechanisms of ischemia-reperfusion (IR) injury in rat liver transplantation, METHODS: Sixty-six healthy m... AIM: To evaluate immunological protection of nitric ox- ide (NO) in hepatopulmonary syndrome and probable mechanisms of ischemia-reperfusion (IR) injury in rat liver transplantation, METHODS: Sixty-six healthy male Wistar rats were randomly divided into three groups (11 donor/recipi-ent pairs). In group 11, organ preservation solution was lactated Ringer's solution with heparin 10 000/μL at 4℃. In groups I and 111, the pLeservation solution added, respectively, L-arginine or Ng-L-arginine methyl ester (L-NAME) (1 mmol/L) based on group 11, and recipients were injected with L-arginine or L-NAME (50 mg/kg) in the anhepatic phase. Grafted livers in each group were stored for 6 h and implanted into recipi- ents. Five rats were used for observation of postopera- tive survival in each group. The other six rats in each group were used to obtain tissue samples, and execut- ed at 3 h and 24 h after transplantation. The levels of alanine aminotransferase (ALT), tumor necrosis factor (TNF)-a and NO metabolites (NOx) were detected, and expression of NO synthase, TNF-α and intercellular adhesion molecule 1 (ICAM-1) was examined by tri- phosphopyridine nucleotide diaphorase histochemical and immunohistochemical staining. RESULTS: By supplementing L-arginine to strengthen the NO pathway, a high survival rate was achieved and hepatic function was improved. One-week sur- viral rate of grafted liver recipients in group I was significantly increased (28.8 4±36.6 d ys 4 4±1.7 d, P 〈 0.01) as compared with groups 11 and Ill. Serum levels of ALT in group ] were 2-7 times less than those in groups 11 and 11I (P 〈 0.01). The cyclic guanosine monophosphate (cGMP) levels in liver tissue and NOx in group I were 3-4 times higher than those of group 11 after 3 h and 24 h reperfusion, while in group ]]], they were significantly reduced as compared with those in group ]1 (P 〈 0.01). The levels of TNF-(z in group I were significantly lower than in group Ⅱ after 3 h and 24 h reperfusion (P 〈 0.01), while being sig- nificantly higher in group Ⅲ than group Ⅱ (P 〈 0.01). Histopathology revealed more severe tissue damage in graft liver and lung tissues, and a more severe in- flammatory response of the recipient after using NO synthase inhibitor, while the pathological damage to grafted liver and the recipient's lung tissues was signifi-cantly reduced in group I after 3 h and 24 h reperfu- sion. A small amount of constitutive NO synthase (cNOS) was expressed in liver endothelial cells after 6 h cold storage, but there was no expression of inducible NO synthase (iNOS). Expression of cNOS was particularly significant in vascular endothelial cells and liver cells at 3 h and 24 h after reperfusion in group Ⅱ but expres- sion of iNOS and ICAM-1 was low in group I. There was diffuse strong expression of ICAM-1 and TNF-α in group Ⅱ at 3 h after reperfusion. CONCLUSION: The NO/cGMP pathway may be critical in successful organ transplantation, especially in treat- ing hepatopulmonary syndrome during cold IR injury in rat orthotopic liver transplantation. 展开更多
关键词 Nitric oxide Nitric oxide synthase Immu-noregulatory Hepatopulmonary syndrome Ischemia-reperfusion injury Orthotopic liver transplantation
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Wind Power Related Policies and Regulations in China--Status, Problems and Solutions
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作者 Qiankun Wang 《Journal of Energy and Power Engineering》 2013年第5期963-971,共9页
This article identifies urgent problems regarding to China's wind power development from the perspective of wind power planning, construction and operation. China's wind power has been experiencing rapid growth sinc... This article identifies urgent problems regarding to China's wind power development from the perspective of wind power planning, construction and operation. China's wind power has been experiencing rapid growth since the enactment of Renewable Energy Law in 2006, but there are also some problems with respect to the coordinated development of wind power and power grids. By learning experience from United States, Germany, Spain and Denmark, some suggestions are systematically put forward for the improvement of wind power related policies and regulations. By taking a neutral position and taking into account interests of all stakeholders, this article has proposed overall, comprehensive and system-wide political and regulatory solutions to the current problems in wind power development. Firstly, unified planning should be implemented. Secondly, administrative procedures and approval process for grid projects should be streamlined and simplified. Thirdly, Mechanism for ancillary service should be set up to motivate conventional units to operate in a more flexible way. Fourthly, peak/valley or even negative feed-in tariff should be introduced stepwise. Fifthly, pilot projects of heating with wind power should be accelerated. 展开更多
关键词 Wind power POLICY regulation.
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Recent advances on smart grid technology and renewable energy integration 被引量:9
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作者 MEI ShengWei CHEN LaiJun 《Science China(Technological Sciences)》 SCIE EI CAS 2013年第12期3040-3048,共9页
With the integration of renewable energy generations and other related changes,the smart grid,or the future electric power system,is confronted with new challenges and opportunities.Therefore,it is a promising subject... With the integration of renewable energy generations and other related changes,the smart grid,or the future electric power system,is confronted with new challenges and opportunities.Therefore,it is a promising subject in electrical engineering and much work has been done on it.This paper reviews the recent advances on the technologies of smart grid and renewable energy integration,from the aspects of modeling,simulation,protection and control,stability,operation,and planning. 展开更多
关键词 smart grid renewable energy integration MODELING SIMULATION protection and control STABILITY operation PLANNING
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One-step synthesis of cobalt,nitrogen-codoped carbon as nonprecious bifunctional electrocatalyst for oxygen reduction and evolution reactions 被引量:8
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作者 Sijie Guo Yanmei Yang +4 位作者 Naiyun Liu Shi Qiao Hui Huang Yang Liu Zhenhui Kang 《Science Bulletin》 SCIE EI CAS CSCD 2016年第1期68-77,共10页
It is highly desired for the development of efficient bifunctional electrocatalyst for both oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) in renewable energy technologies. In this work, cobal... It is highly desired for the development of efficient bifunctional electrocatalyst for both oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) in renewable energy technologies. In this work, cobalt, nitrogen-codoped carbon was prepared by a facile one-step method and demonstrated to exhibit good elec- trocatalytic performance for ORR and OER via a complete four-electron process. Besides, the catalyst prepared at 900 ℃ also displayed excellent stability for both ORR and OER. Furthermore, the origin of catalytic activity was also explored, which was attributed to the synergistic effects of metallic Co and quaternary N. 展开更多
关键词 Carbon quantum dots Bifunctionalcatalyst Electrochemical performance
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nNOS expression of hippocampal neurons in aged rats after brain ischemia/reperfusion and its role in DND development 被引量:7
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作者 杨传红 赖晃文 +2 位作者 詹纯列 肖育华 郑文岭 《Chinese Journal of Traumatology》 CAS 2002年第4期232-236,共5页
Objective: To study the role of neuronal nitric oxide synthase (nNOS) in aged rats hippocampal delayed neuronal death (DND) following brain ischemia. Methods: Models of incomplete brain ischemia were induced by clippi... Objective: To study the role of neuronal nitric oxide synthase (nNOS) in aged rats hippocampal delayed neuronal death (DND) following brain ischemia. Methods: Models of incomplete brain ischemia were induced by clipping common carotid artery. A total of 46 aged SD rats were divided into 8 groups: normal control group (Group A, n=5), sham operation group (Group B, n=5), reperfusion 1, 6, 12, 24, 48, and 96 hours groups after brain ischemia for 30 minutes (Group C, D, E, F, G, and H, n=6/group). The expression of nNOS was examined by immunohistochemistry and neuronal ultrastructural changes were observed by the transmission electron microscopy (TEM) at different time points after reperfusion. Results: Immunohistochemistry showed that nNOS expression in the hippocampal neurons was high in Group E, low expression in Group D, moderate expression in Group F and G. There was nearly no expression of nNOS in Group A, B, C, and H. Ultrastructure of hippocampal neurons was damaged more severely in reperfusion over 24 hours groups. Conclusions: Nitric oxide (NO) may be one of the important factors in inducing DND after ischemia/reperfusion. 展开更多
关键词 Rats Nitric oxide synthase Ischemia reperfusion Hippocampus neurons
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Study of L-arginine-nitric oxide pathway in ischemia-reperfusion injured limbs in rats 被引量:3
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作者 朱立军 黄耀添 裴国献 《Chinese Journal of Traumatology》 CAS 2002年第1期16-20,共5页
Objective: To observe the change of nitric oxide (NO) levels in the blood and the morphological change of the muscles in the limbs of rats during the (IR) injury and after being intervened by L-arginine (L-Arg) and L-... Objective: To observe the change of nitric oxide (NO) levels in the blood and the morphological change of the muscles in the limbs of rats during the (IR) injury and after being intervened by L-arginine (L-Arg) and L-nitroarginine (L-NNA). Methods: Sixty-six male Sprague-Dawley (SD) rats were used and grouped into the normal controls, the sham injury controls, the IR injury group and the intervention groups (L-Arg group and L-NNA group). After 6 hours of ischemia, followed by reperfusion for 3, 12 or 24 hours, the samples in the IR injury group were obtained. The rats in the intervention groups were given L-Arg (100 mmol/L) and L-NNA (10 mmol/L), respectively, through the abdominal cavity. Then the anterior tibial muscle in the right limb was obtained for histological examination, the anterior tibial muscle in the left limb for ultrastructure observation and the blood for assay of NO in all the rats. NO was assayed by indirect measurement of NO 2 -/NO 3 - with Griess method. Results: There was no significant difference of NO between the normal controls and the sham injury controls (P> 0.05). But NO significantly decreased in the IR injury group (P< 0.01), and further decreased with reperfusion (P< 0.01) and reached the lowest point at 12 hours after reperfusion. The level of NO in the L-Arg group was significantly higher than that in the IR injury group (P< 0.01), but was not significantly different from that in the controls (P> 0.05). In the L-NNA group, NO decreased to the undetectable level (P< 0.01). Histological examination and ultrastructure observation showed the muscles were normal in the control groups. After 6 hours of ischemia, the skeletal muscles displayed injuries, and they were most severely injured after 12 hours of reperfusion. In the L-Arg group, the skeletal muscles were less injured, while in the L-NNA group, the injury was similar to that in the IR injury group. Conclusions: When the limbs of the rats sustain IR, NO in the blood decreases. Meanwhile, the muscles in the limbs are injured. When L-Arg is given, NO in the blood is restored and the muscles are protected. When L-NNA completely inhibits NO, no protection of the muscles is shown. 展开更多
关键词 Reperfusion injury LIMBS Nitric oxide
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Protection of carbon monoxide-releasing molecule against lung injury induced by limb ischemia-reperfusion 被引量:9
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作者 周君琳 李钢 +3 位作者 海涌 关立 黄新莉 孙鹏 《Chinese Journal of Traumatology》 CAS 2009年第2期71-76,共6页
Objective: To observe the role and mechanism of CO- releasing molecule (CORM)-2 in lung injury induced by ischemia-reperfusion (IR) of hind limbs in rats. Methods: Arat model of lung injury induced by IR of hind... Objective: To observe the role and mechanism of CO- releasing molecule (CORM)-2 in lung injury induced by ischemia-reperfusion (IR) of hind limbs in rats. Methods: Arat model of lung injury induced by IR of hind limbs was established. A total of 40 Sprague Dawley (SD) rats were randomly divided into 5 groups (n = 8): sham, sham + CORM-2, IR, IR + CORM-2 and IR + dimethyl sulfoxide (DMSO). Rats in the IR group received hind limb ischemia for 2 hours and reperfusion for 2 hours, rats in the sham group underwent sham surgery without infrarenal aorta occlusion, rats in the IR+CORM-2 group and in the sham + CORM-2 group were given CORM-2 (10 μmol/kg intravenous bolus) 5 minutes before reperfusion or at the corresponding time points, while rats in the IR + DMSO group was treated with the same dose of vehicle (DMSO) at the same time. The lung tissue structure, polymorphonuclear neutrophil (PMN) count, wet-to-dry weight ratio (W/D), malondialdehyde (MDA) content, myeloperoxidase (MPO) activity, intercellular adhesion molecule- 1 (ICAM- 1)expression, I κBα degradation and nuclear factor (NF)-κB activity in the lungs were assessed. Results: As compared with the sham group, lung PMNs number, W/D, MDA content, MPO activity, ICAM-1 expression and NF- κB activity significantly increased in the IR group, but the level of I κBα decresed (P〈0.01). Compared with the IR group, lung PMNs number, W/D, MDA content, MPO activity and ICAM- 1 expression significantly decreased in the IR+COMR-2 group (P〈0.01), while the level of IκBα increased. Conclusions: These data demonstrate that CORM-2 attenuates limb IR-induced lung injury through inhibiting ICAM-1 protein expression, NF-κB pathway and the leu- kocytes sequestration in the lungs following limb IR in rats, suggesting that CORM-2 may be used as a therapeutic agent against lung injury induced by limb IR. 展开更多
关键词 Carbon monoxide LUNG Reperfusioninjury Nuclear factor kappa B
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