With the assistance of the symbolic computation system Maple,rich higher order polynomial-type conservation laws and a sixth order t/x-dependent conservation law are constructed for a generalized seventh order nonline...With the assistance of the symbolic computation system Maple,rich higher order polynomial-type conservation laws and a sixth order t/x-dependent conservation law are constructed for a generalized seventh order nonlinear evolution equation by using a direct algebraic method.From the compatibility conditions that guaranteeing the existence of conserved densities,an integrable unnamed seventh order KdV-type equation is found.By introducing some nonlinear transformations,the one-,two-,and three-solition solutions as well as the solitary wave solutions are obtained.展开更多
Aim To quantitatively determine five nucleosides and nucleobases, including cytidine, uridine, guanosine, adenosine and uracil in different parts of Panax notoginseng. Methods Separation was performed on a Zorbax SB-A...Aim To quantitatively determine five nucleosides and nucleobases, including cytidine, uridine, guanosine, adenosine and uracil in different parts of Panax notoginseng. Methods Separation was performed on a Zorbax SB-Aq column using a gradient elution with mobile phase of 8 mmol^L-1 ammonium acetate aqueous solution （A） and methanol （B）. The assay was carried out at a flow rate of 1 mL·min^-1 at 25 ℃ with the diode-array detection at 260 nm. Results Cytidine, uridine, guanosine, adenosine and uracil had good linearity in the ranges of 1.79 - 57.40 μg·mL^-1 （r^2 = 1.0000）, 3.30 - 105.60 μg·mL^-1 （r^2 = 1.0000）, 3.09 - 98.80 μg·mL^ -1（r^2 = 0.9999）, 2.77 - 88.60 μg·mL^-1 （r^2 = 1.0000） and 0.38 - 12.30 μg·mL ^-1 （r^2 = 1.0000） with average recoveries of 93.9%, 96.5%, 92.7%, 93.2% and 98.8%, respectively. The content of cytidine, uridine, guanosine, adenosine and uracil in different parts of P. notogingeng were significantly different. Conclusion This is the first report on quantitative determination of nucleosides and nucleobases in P notoginseng.展开更多
To establish a method for determining five saponins(notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ammonium glycyrrhizinate) in Glycyrrhizae, Notoginseng and Ginseng, the high performance liq...To establish a method for determining five saponins(notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ammonium glycyrrhizinate) in Glycyrrhizae, Notoginseng and Ginseng, the high performance liquid chromatography with diode array detector(HPLC-DAD) method was applied to an Inertsil ODS-SP column(4.6 mm×250 mm, 5 μm) with a mobile phase consisting of acetonitrile-0.05% phosphoric acid in a gradient elution manner. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelengths were 203 nm and 237 nm, respectively. The linear ranges were 0.700,0—7.000,0 μg for R1(r=1.000,0), 0.751,1— 7.511,4 μg for Rg1(r=1.000,0), 0.677,2—6.771,6 μg for Re(r=1.000,0), 0.733,9—7.339,1 μg for Rb1(r= 1.000,0), and 0.540,0—5.399,8 μg for ammonium glycyrrhizinate(r=0.999,9), respectively. In addition, their average recoveries were 100.28%, 105.83%, 104.09%, 99.36% and 98.54%, respectively. The relative standard deviations(RSDs) of precision, reproducibility and recovery were all less than 1.5%. The results indicate that the method is simple, accurate and reproducible so that it can be used for the simultaneous determination of the five saponins in Chinese patent medicines containing the three kinds of herbs.展开更多
Objective To compare the different therapeutic effect between acupuncture at Shiqizhui （十七椎 EX-B 8） only and multi-acupoints on dysmenorrhea. Methods Thirty-eight cases were randomly divided into asingle acupoin...Objective To compare the different therapeutic effect between acupuncture at Shiqizhui （十七椎 EX-B 8） only and multi-acupoints on dysmenorrhea. Methods Thirty-eight cases were randomly divided into asingle acupoint group and a multi-acupoints group, 19 cases in each group. The single acupoint group was treated by acupuncture at Shfqizhui （十七椎EX-B 8） only, and the multi-acupoints group by acupuncture at Shlqizhui （十七椎EX-B 8）, Sanyinjiao （三阴交SP 6）, Diji （地机 SP 8）, Ciliao （次髎 BL 32）. They were all treated from the first day when sudden intense pain occured, one time each day, for 3 days in each menstrual cycle, the treatment of three menstrual cycles. The therapeutic effect and Visual Analogue Scale （VAS） were compared and the scores of general frequency and severity of dysmenorrhea by using Cox Menstrual Symptom Scale （CMSS） were evaluated. Results The cured rate was 68.4% （13/19） and the effective rate was 31.6% （6/19） in the single acupoint group, being similar to 78.9% （15/19） and 21.1% （4/19） in the muhi-acupoints group （P〉0.05）. VAS and the scores of general frequency and severity of dysmenorrhea were all significantly decreased after treatment in both groups （all P〈0.001）, with no significant difference between the two groups （all P〉0.05）. Conclusion Acupuncture at Shiqizhui （十七椎 EX-B 8） only can be as effective as selecting multi-acupoints to cure primary dysmenorrhea.展开更多
文摘With the assistance of the symbolic computation system Maple,rich higher order polynomial-type conservation laws and a sixth order t/x-dependent conservation law are constructed for a generalized seventh order nonlinear evolution equation by using a direct algebraic method.From the compatibility conditions that guaranteeing the existence of conserved densities,an integrable unnamed seventh order KdV-type equation is found.By introducing some nonlinear transformations,the one-,two-,and three-solition solutions as well as the solitary wave solutions are obtained.
文摘Aim To quantitatively determine five nucleosides and nucleobases, including cytidine, uridine, guanosine, adenosine and uracil in different parts of Panax notoginseng. Methods Separation was performed on a Zorbax SB-Aq column using a gradient elution with mobile phase of 8 mmol^L-1 ammonium acetate aqueous solution （A） and methanol （B）. The assay was carried out at a flow rate of 1 mL·min^-1 at 25 ℃ with the diode-array detection at 260 nm. Results Cytidine, uridine, guanosine, adenosine and uracil had good linearity in the ranges of 1.79 - 57.40 μg·mL^-1 （r^2 = 1.0000）, 3.30 - 105.60 μg·mL^-1 （r^2 = 1.0000）, 3.09 - 98.80 μg·mL^ -1（r^2 = 0.9999）, 2.77 - 88.60 μg·mL^-1 （r^2 = 1.0000） and 0.38 - 12.30 μg·mL ^-1 （r^2 = 1.0000） with average recoveries of 93.9%, 96.5%, 92.7%, 93.2% and 98.8%, respectively. The content of cytidine, uridine, guanosine, adenosine and uracil in different parts of P. notogingeng were significantly different. Conclusion This is the first report on quantitative determination of nucleosides and nucleobases in P notoginseng.
基金Supported by Plan Issue of Shanghai Science &Technology Committee(No.13401901100)
文摘To establish a method for determining five saponins(notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ammonium glycyrrhizinate) in Glycyrrhizae, Notoginseng and Ginseng, the high performance liquid chromatography with diode array detector(HPLC-DAD) method was applied to an Inertsil ODS-SP column(4.6 mm×250 mm, 5 μm) with a mobile phase consisting of acetonitrile-0.05% phosphoric acid in a gradient elution manner. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelengths were 203 nm and 237 nm, respectively. The linear ranges were 0.700,0—7.000,0 μg for R1(r=1.000,0), 0.751,1— 7.511,4 μg for Rg1(r=1.000,0), 0.677,2—6.771,6 μg for Re(r=1.000,0), 0.733,9—7.339,1 μg for Rb1(r= 1.000,0), and 0.540,0—5.399,8 μg for ammonium glycyrrhizinate(r=0.999,9), respectively. In addition, their average recoveries were 100.28%, 105.83%, 104.09%, 99.36% and 98.54%, respectively. The relative standard deviations(RSDs) of precision, reproducibility and recovery were all less than 1.5%. The results indicate that the method is simple, accurate and reproducible so that it can be used for the simultaneous determination of the five saponins in Chinese patent medicines containing the three kinds of herbs.
基金Supported by Chinese Medicine Project of "Eleventh Five-Year" National Technology Support Program: 2006 BAI 12 B 06
文摘Objective To compare the different therapeutic effect between acupuncture at Shiqizhui （十七椎 EX-B 8） only and multi-acupoints on dysmenorrhea. Methods Thirty-eight cases were randomly divided into asingle acupoint group and a multi-acupoints group, 19 cases in each group. The single acupoint group was treated by acupuncture at Shfqizhui （十七椎EX-B 8） only, and the multi-acupoints group by acupuncture at Shlqizhui （十七椎EX-B 8）, Sanyinjiao （三阴交SP 6）, Diji （地机 SP 8）, Ciliao （次髎 BL 32）. They were all treated from the first day when sudden intense pain occured, one time each day, for 3 days in each menstrual cycle, the treatment of three menstrual cycles. The therapeutic effect and Visual Analogue Scale （VAS） were compared and the scores of general frequency and severity of dysmenorrhea by using Cox Menstrual Symptom Scale （CMSS） were evaluated. Results The cured rate was 68.4% （13/19） and the effective rate was 31.6% （6/19） in the single acupoint group, being similar to 78.9% （15/19） and 21.1% （4/19） in the muhi-acupoints group （P〉0.05）. VAS and the scores of general frequency and severity of dysmenorrhea were all significantly decreased after treatment in both groups （all P〈0.001）, with no significant difference between the two groups （all P〉0.05）. Conclusion Acupuncture at Shiqizhui （十七椎 EX-B 8） only can be as effective as selecting multi-acupoints to cure primary dysmenorrhea.
