Purpose of the study was to demonstrate the ability of Pleurotus eryngii （PE） extracts to stimulate the production of nitric oxide （NO） and TNF-α as well as to evaluate the cell viability by the use of chicken pe...Purpose of the study was to demonstrate the ability of Pleurotus eryngii （PE） extracts to stimulate the production of nitric oxide （NO） and TNF-α as well as to evaluate the cell viability by the use of chicken peripheral blood mononuclear cells （PBMCs） and RAW 264.7 macrophages in vitro. The PE contained 341.0 ± 14.5 mg/g DW, 78,2 ± 4.1 Bg/g DW and 38.4 ＋ 3.3 mg/g DW of crude polysaccharide, adenosine and crude triterpenoid contents, respectively. The results of cell viability assay showed that 95,0% of chicken PBMCs and RAW 264.7 macrophages were survived under the treatment of 9.6 mg/mL of PE extracts by microculture tetrazolium assays （MTAs）. Moreover, the cell inhibitory activity did not show the 50% cyto-toxicity effect （ICs0）. In addition, no production by stimulated chicken PBMCs and RAW 264.7 macrophages exhibited individually the linear relations with the concentration of PE extracts, and they were approximately 65 μM and 45 p.M in 1600 μg/mL, respectively. The tendency with the release of TNF-α by RAW 264.7 maerophages also corresponded with concentration of PE extracts. It is concluded, PE extracts act as an immunostimulant for both tested cells but have no serious effect on inhibiting chicken PBMCs viability, and therefore it may be suggested as immune supplements for broilers.展开更多
文摘Purpose of the study was to demonstrate the ability of Pleurotus eryngii （PE） extracts to stimulate the production of nitric oxide （NO） and TNF-α as well as to evaluate the cell viability by the use of chicken peripheral blood mononuclear cells （PBMCs） and RAW 264.7 macrophages in vitro. The PE contained 341.0 ± 14.5 mg/g DW, 78,2 ± 4.1 Bg/g DW and 38.4 ＋ 3.3 mg/g DW of crude polysaccharide, adenosine and crude triterpenoid contents, respectively. The results of cell viability assay showed that 95,0% of chicken PBMCs and RAW 264.7 macrophages were survived under the treatment of 9.6 mg/mL of PE extracts by microculture tetrazolium assays （MTAs）. Moreover, the cell inhibitory activity did not show the 50% cyto-toxicity effect （ICs0）. In addition, no production by stimulated chicken PBMCs and RAW 264.7 macrophages exhibited individually the linear relations with the concentration of PE extracts, and they were approximately 65 μM and 45 p.M in 1600 μg/mL, respectively. The tendency with the release of TNF-α by RAW 264.7 maerophages also corresponded with concentration of PE extracts. It is concluded, PE extracts act as an immunostimulant for both tested cells but have no serious effect on inhibiting chicken PBMCs viability, and therefore it may be suggested as immune supplements for broilers.
