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规模化猪场母猪“三炎”防治措施 被引量:1
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作者 胡勇 杨士洪 李永华 《兽医导刊》 2014年第11X期92-92,共1页
母猪“子宫内膜炎、阴道炎、乳炎”简称“三炎”,成为规模化猪场的常见多发病。结合临床实践发现,“三炎”之间存在某种必然联系,掌握母猪三炎综合症的发病机制和病理特点,制定相应的预防控制措施,在实践中取得了理想的预防控制效... 母猪“子宫内膜炎、阴道炎、乳炎”简称“三炎”,成为规模化猪场的常见多发病。结合临床实践发现,“三炎”之间存在某种必然联系,掌握母猪三炎综合症的发病机制和病理特点,制定相应的预防控制措施,在实践中取得了理想的预防控制效果。同时,发现母猪“三炎”综合症药物治疗的统一规律,总结治疗“三炎”的处方,对规模化猪场防治和净化母猪“三炎”综合征具有极好的现实意义。 展开更多
关键词 规模化 猪场 母猪 “三炎” 综合征 防制措施
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三黄消炎方结合银敷料湿性换药治疗糖尿病足感染(多重耐药菌)临床观察
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作者 陈向红 何红梅 +1 位作者 赵娜 王建丽 《辽宁中医杂志》 CAS 2023年第6期82-84,共3页
目的观察三黄消炎方结合银敷料湿性换药治疗糖尿病足感染(多重耐药菌)临床效果。方法研究纳入86例糖尿病足感染(多重耐药菌)患者(2019年12月—2021年6月收治),将患者以随机数字表法分成对照组与观察组,各43例,对照组患者银敷料湿性换药... 目的观察三黄消炎方结合银敷料湿性换药治疗糖尿病足感染(多重耐药菌)临床效果。方法研究纳入86例糖尿病足感染(多重耐药菌)患者(2019年12月—2021年6月收治),将患者以随机数字表法分成对照组与观察组,各43例,对照组患者银敷料湿性换药治疗治疗,观察组患者三黄消炎方结合银敷料湿性换药治疗。观察疗效、治疗前后中医证候(创面疼痛、溃破腐烂、皮肤暗紫、身热口干、便秘溲赤等)积分变化及创面面积变化、白细胞介素-1(IL-1)和肿瘤坏死因子-α(TNF-α)的水平变化、不良反应。结果观察组有效率为93.02%(40/43),对照组为76.74%(33/43),观察组有效率更高(P<0.05);治疗前,两组患者中医证候(创面疼痛、溃破腐烂、皮肤暗紫、身热口干、便秘溲赤等)积分、创面面积、IL-1及TNF-α水平比较无统计学意义(P>0.05)。治疗后各组患者中医证候(创面疼痛、溃破腐烂、皮肤暗紫、身热口干、便秘溲赤等)积分、创面面积、IL-1及TNF-α水平均改善,且观察组水平优于对照组(P<0.05);两组均无不良反应。结论三黄消炎方结合银敷料湿性换药治疗糖尿病足感染(多重耐药菌)临床效果显著,患者创面愈合好,安全可靠,值得应用。 展开更多
关键词 糖尿病足感染(多重耐药菌) 黄消 银敷料湿性换药 创面面积 中医证候积分 疗效
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黄马酊联合三黄消炎方对糖尿病足溃疡创面修复的作用分析 被引量:7
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作者 赵莉娟 周莎 +1 位作者 冷静 余晓云 《中华中医药学刊》 CAS 北大核心 2021年第10期159-162,共4页
目的探讨黄马酊联合三黄消炎方对糖尿病足溃疡创面修复的作用。方法选择医院于2017年12月—2019年12月收治的糖尿病足溃疡患者98例,依据随机数字表法随机分为观察组49例与对照组49例。对照组患者给予黄马酊治疗,观察组在对照组基础上结... 目的探讨黄马酊联合三黄消炎方对糖尿病足溃疡创面修复的作用。方法选择医院于2017年12月—2019年12月收治的糖尿病足溃疡患者98例,依据随机数字表法随机分为观察组49例与对照组49例。对照组患者给予黄马酊治疗,观察组在对照组基础上结合三黄消炎方治疗。两组疗程均为4周。比较两组治疗疗效,创面愈合情况,治疗前后溃疡面积、炎症因子、血清血管内皮生长因子(VEGF)、基质蛋白酶组织抑制剂(TIMP-1)、基质金属蛋白酶-2(MMP-2)水平变化,及不良反应情况。结果观察组总有效率(95.92%,47/49)高于对照组(81.63%,40/49)(P<0.05)。观察组创面愈合率高于对照组(P<0.05)。两组治疗后溃疡面积较治疗前减少(P<0.05);观察组治疗后溃疡面积小于对照组(P<0.05)。两组治疗后血清TNF-α、IL-1和ICAM-1水平较治疗前降低(P<0.05);观察组治疗后血清TNF-α、IL-1和ICAM-1水平低于对照组(P<0.05)。两组治疗后血清VEGF和TIMP-1水平较治疗前升高,血清MMP-2水平较治疗前降低(P<0.05);观察组治疗后血清VEGF和TIMP-1水平高于对照组,血清MMP-2水平低于对照组(P<0.05)。两组均未发生明显不良反应。结论黄马酊联合三黄消炎方对糖尿病足溃疡创面修复疗效良好,不仅可缩小创面面积,且可减轻炎症反应,下调MMP-2表达,及上调TIMP-1和VEGF表达。 展开更多
关键词 黄马酊 黄消 糖尿病足溃疡 创面修复
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Chylous ascites secondary to hyperlipidemic pancreatitis with normal serum amylase and lipase 被引量:24
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作者 Fahmi Yousef Khan Issa Matar 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第3期480-482,共3页
A 54-year old man with a family history of hyperlipidemia was admitted with a 12 h history of severe generalized abdominal pain associated with nausea, vomiting and abdominal distension. Examination of the abdomen rev... A 54-year old man with a family history of hyperlipidemia was admitted with a 12 h history of severe generalized abdominal pain associated with nausea, vomiting and abdominal distension. Examination of the abdomen revealed tenderness in the periumblical area with shifting dullness. Serum pancreatic amylase was 29 IU/L and lipase 44 IU/L, triglyceride 36.28 mmol/L. Ultrasound showed ascites. CT of the abdomen with contrast showed inflammatory changes surrounding the pancreas consistent with acute pancreatitis. Ultrasound (US) guided abdomen paracentesis yielded a milky fluid with high triglyceride content consistent with chylous ascites. The patient was kept fasting and intravenous fluid hydration was provided. Meperidine was administered for pain relief. On the following days the patient’s condition improved and he was gradually restarted on a low-fat diet, and fat lowering agent (gemfibrozil) was begun, 600 mg twice a day. On d 14, abdomen US was repeated and showed fluid free peritoneal cavity. The patient was discharged after 18 d of hospitalization with 600 mg gemfibrozil twice a day. At the time of discharge, the fasting triglyceride was 4.2 mmol/L. After four weeks the patient was seen in the clinic, he was well. 展开更多
关键词 AMYLASE Chylous ascites Hyperlipidemic pancreatitis
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Large-vessel thrombosis in intestinal Behet's disease complicated with myelodysplastic syndrome and trisomy 8 被引量:2
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作者 Huang-Chi Chen Ying-Ming Chiu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第10期1137-1140,共4页
Behet's disease is characterized by recurrent oral ulcers, genital ulcers, uveitis and skin lesions. Myelodysplastic syndrome (MDS) is characterized by problems due to ineffective hematopoiesis. Several studies ha... Behet's disease is characterized by recurrent oral ulcers, genital ulcers, uveitis and skin lesions. Myelodysplastic syndrome (MDS) is characterized by problems due to ineffective hematopoiesis. Several studies have identified a relationship between MDS and Behet's disease, especially intestinal Behet's disease. Trisomy 8 seems to play an important role in these disorders as well. The present case was a 24-year-old woman who had a huge tonsil ulcer with initial symptoms of odynophagia and intermittent fever. We also noted folliculitis on her upper back. Five days later, she began to experience diarrhea and abdominal pain. Abdominal computed tomography and subsequent surgery revealed ileum perforation and enterocolitis with multiple ulcers. Later, she was admitted again for a vulvar suppurative ulcer and suspicious Bartholin's cyst infection. The patient's clinical presentations met the criteria for Behet's disease. Six months after the bowel perforation event, we noted the development of pancytopenia in a routine laboratory examination. All the examinations led to the diagnosis of MDS with trisomy 8. The most unusual finding was that multiple large vessel thrombi developed during follow-up. Previous studies have suggested that trisomy 8 in MDS leads to concurrent intestinal Behet's disease. Moreover, the inflammatory and immune genes related to thrombus formation are overexpressed in cases of MDS with trisomy 8. Trisomy 8 must play a role in thrombosis. Further studies are needed to help clarify the pathophysiology and pathogenesis of these disorders. 展开更多
关键词 Behcet's disease Myelodysplastic syndrome Trisomy 8 Intestinal ulcers THROMBOSIS
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Diagnostic evaluation of acute pancreatitis in two patients with hypertriglyceridemia 被引量:12
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作者 Yoshifumi Okura KozoHayashi +3 位作者 TetsujiShingu GoroKajiyama Yoshiyuki Nakashima Keijiro Saku 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第24期3691-3695,共5页
We present two diagnostically challenging cases of acute pancreatitis with hypertriglyceridemia accompanied with chylomicronemia caused with a deficiency of lipoprotein lipase and with the presence of type Ⅴ hyperlip... We present two diagnostically challenging cases of acute pancreatitis with hypertriglyceridemia accompanied with chylomicronemia caused with a deficiency of lipoprotein lipase and with the presence of type Ⅴ hyperlipidemia. Both cases suffered from acute abdomen following the ingestion of fatty food and revealed the increase in parameters of inflammation without significant elevation of serum amylase levels.The imaging examination of ultrasonography could not detect significant findings of acute pancreatitis and a computer tomography scan eventually confirmed the findings of acute pancreatitis.Both cases responded to a low fat diet and administration of a cholecystokinin receptor antagonist,exhibiting a relief of abdominal symptoms.As in the present cases with acute abdomen following the ingestion of fatty food,the identification of serum hypertriglyceridemia and an abdominal computer tomography scan might be useful in establishing the diagnosis of acute pancreatitis and in developing the therapeutic regimen,when hypertriglyceridemia interferes with the evaluation of pancreatic enzyme activities and ultrasound examination provides poor pancreatic visualization. 展开更多
关键词 Acute Disease ADULT FEMALE Humans HYPERTRIGLYCERIDEMIA MALE Middle Aged PANCREATITIS TRIGLYCERIDES
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Protective effect of ilomastat on trinitrobenzenesulfonic acid-induced ulcerative colitis in rats 被引量:1
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作者 Ying-De Wang Wei Wang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第37期5683-5688,共6页
AIM: To evaluate the protective effects of ilomastat, an exogenous matrix metalloproteinase (MMP) inhibitor, on trinitrobenzenesulfonic acid (TNB)-induced ulcerative colitis (UC) in rats. METHODS: Male SD rats... AIM: To evaluate the protective effects of ilomastat, an exogenous matrix metalloproteinase (MMP) inhibitor, on trinitrobenzenesulfonic acid (TNB)-induced ulcerative colitis (UC) in rats. METHODS: Male SD rats were randomly divided into model group, protective groups A and B, and normal control group. Rats in the model group received only intra-colonic TNB. Rats in the protective groups A and B received intra-peritoneal ilomastat of 10 mg/kg and 20 mg/kg, respectively, beside TNB. Rats in the normal control group received only intra-colonic normal saline. After 3 wk, segments of colon were obtained. RT-PCR and immunohistochemistry were used to examine the expression of MMP-1 and TIMP-1. Hematoxylin-eosin (HE) staining was used for pathological study. RESULTS: The model of UC was successfully induced in rats. Inflammation of colonic mucosa greatly improved in protective groups A and B. Expression of MMP-1 and TIMP-1 in the model group, protective groups A and B was significantly higher than that in the normal control group (P 〈 0.0001) with MMP-1 expression increased more significantly than TIMP-1 expression. Expression of MMP-1 in protective groups A and B was significantly lower than that in the model group (P 〈 0.0001). Expression of MMP-1 in protective group B was significantly lower than that in protective group A (P 〈 0.0001). CONCLUSION: IIomastat improves TNB-induced UC in rats by inhibiting the MMP-1 activity. 展开更多
关键词 Ulcerative colitis Trinitrobenzenesulfonic acid Matrix metalloproteinase-1 Tissue inhibitors of matrix metalloproteinase-1 Ilomastat
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Protective effect of notoginsenoside and tanshinone IIA on inflammation-related colorectal cancer mice and the inhibition effect on COX-2 expression 被引量:2
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作者 CAO Wen ZHOU Xiaoqing 《Digital Chinese Medicine》 2021年第1期54-63,共10页
Objective To explore the preventive effects and possible mechanisms of action of notoginsenoside(NGS)and tanshinone IIA(TSN)in inflammation-related colorectal cancer(IRCC)in mice.Methods Eighty-eight male C57BL/6 mice... Objective To explore the preventive effects and possible mechanisms of action of notoginsenoside(NGS)and tanshinone IIA(TSN)in inflammation-related colorectal cancer(IRCC)in mice.Methods Eighty-eight male C57BL/6 mice were randomly assigned to 11 groups(n=8 each group).Azomethane oxide+dextran sulfate(AOM+DSS)model control(model),NGS lowdose(l-NGS),NGS medium-dose(m-NGS),NGS high-dose(h-NGS),TSN low-dose(l-TSN),TSN medium-dose(m-TSN),TSN high-dose(h-TSN),(NGS+TSN)low-dose[l-(NGS+TSN)],(NGS+TSN)medium-dose[m-(NGS+TSN)],(NGS+TSN)high-dose[h-(NGS+TSN)],and blank groups were established.The first 10 groups were intraperitoneally injected with AOM to induce inflammatory colon cancer,whereas the blank group was intraperitoneally injected with 0.9%NaCl solution.The first 10 groups drank a 2.5%sodium DSS aqueous solution continuously from day 5 for three cycles(one cycle:five days,every three weeks),and the blank group was allowed free access to water.Drug groups were administered NGS(low,medium,or high dose),TSN(low,medium,or high dose),or NGS+TSN(low,medium,or high dose),and the model and blank groups were administered saline by lavage until the end of the experiment.The general activity,body weight,and survival rate of and incidence of adenocarcinoma in mice were detected and the expression of cyclooxygenase 2(COX-2)was detected by immunohistochemistry.Results(1)The survival rate of mice with IRCC in the h-NGS,m-TSN,h-TSN,m-(NGS+TSN),and h-(NGS+TSN)groups was significantly increased than that in other groups(P<0.05).(2)The incidence of tumors in the h-(NGS+TSN),m-TSN,and l-NGS groups was significantly lower than that in the model group(P<0.05).(3)The expression level of COX-2 in tumor tissues of mice in the m-(NGS+TSN)and h-(NGS+TSN)groups was significantly lower than that in the model group(P<0.05).Conclusion Tumor formation was inhibited by m-TSN and h-(NGS+TSN)treatments in mice with IRCC,and h-(NGS+TSN)treatment inhibited the COX-2 pathway. 展开更多
关键词 Colorectal cancer Cyclooxygenase-2 (COX-2) INFLAMMATION Notoginsenoside Tanshinone ⅡA
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Effect of admission hypertriglyceridemia on the episodes of severe acute pancreatitis 被引量:61
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作者 Li-Hui Deng Ping Xue Qing Xia Xiao-Nan Yang Mei-Hua Wan 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第28期4558-4561,共4页
AIM: To investigate the effect of admission hypertriglyceridemia (HTG) on the episodes of severe acute pancreatitis (SAP). METHODS: One hundred and seventy-six patients with SAP were divided into HTG group (n =... AIM: To investigate the effect of admission hypertriglyceridemia (HTG) on the episodes of severe acute pancreatitis (SAP). METHODS: One hundred and seventy-six patients with SAP were divided into HTG group (n = 45) and control group (n = 131) according to admission triglyceride (TG) ≥ 5.65 mmol/L and 〈 5.65 retool/L, respectively. Demographics, etiology, underlying diseases, biochemical parameters, Ranson' s score, acute physiology and chronic heath evaluation Ⅱ (APACHE Ⅱ) score, Balthazar's computed tomography (CT) score, complications and mortality were compared. Correlation between admission TG and 24-h APACHE Ⅱ score was analyzed. RESULTS: SAP patients with HTG were younger (40.8 ± 9.3 years vs 52.6 ± 13.4 years, P 〈 0.05) with higher etiology rate of overeating, high-fat diet (40.0% vs 14.5%, P 〈 0.05) and alcohol abuse (46.7% vs 23.7%, P 〈 0.01), incidence rate of hypocalcemia (86.7% vs 63.4%, P 〈 0.01) and hypoalbuminemia (84.4% vs 60.3%, P 〈 0.01), 24-h APACHE Ⅱ score (13.6 ± 5.7 vs 10.7 ± 4.6, P 〈 0.01) and admission serum glucose (17.7 ± 7.7 vs 13.4 ± 6.1, P 〈 0.01), complication rate of renal failure (51.1% vs 16.8%, P 〈 0.01), shock (37.9% vs 14.5%, P 〈 0.01) and infection (37.4% vs 18.3%, P 〈 0.01) and mortality (13.1% vs 9.1%, P 〈 0.01). Logistic regression analysis showed a positive correlation between admission TG and 24-h APACHE Ⅱ score (r = 0.509, P = 0.004).CONCLUSION: The clinical features of SAP patients with HTG are largely consistent with previous studies, HTG aggravates the episodes of SAP. 展开更多
关键词 Clinical study HYPERTRIGLYCERIDEMIA Severe acute pancreatitis Clinical features OUTCOME
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Curcumin-attenuated trinitrobenzene sulphonic acid induces chronic colitis by inhibiting expression of cyclooxygenase-2 被引量:8
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作者 Hua liang Chang-Sheng Deng Ming Zhang Jian Xia 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第24期3848-3853,共6页
AIM: To explore the possible mechanisms of curcumin in rat colitis induced by trinitrobenzene sulfonic (TNBS) acid. METHODS: Rats with TNBS acid-induced colitis were treated with curcumin (30 mg/kg or 60 mg/kg pe... AIM: To explore the possible mechanisms of curcumin in rat colitis induced by trinitrobenzene sulfonic (TNBS) acid. METHODS: Rats with TNBS acid-induced colitis were treated with curcumin (30 mg/kg or 60 mg/kg per day ip). Changes of body weight and histological scores as well as survival rate were evaluated. Leukocyte infiltration was detected by myeloperoxidase (MPO) activity assay. The expression of cyclooxygenase-2 (COX-2) was detected by RT-PCR and Western blot. Inflammation cytokines were determined by RT-PCR. Local concentration of prostaglandin E2 (PGE2) in colon mucosa was determined by ELISA. RESULTS: Curcumin improved survival rate and histological image, decreased the macroscopic scores and MPO activity. Also curcumin reduced the expression of COX-2 and inflammation cytokines. In addition, treatment with curcumin increased the PGE2 level. CONCLUSION: Curcumin has therapeutic effects on TNBS acid-induced colitis, the mechanisms seem to be related to COX-2 inhibition and PGE2 improvement. 展开更多
关键词 COLITIS CURCUMIN CYCLOOXYGENASE-2 PGE2 TNBS acid
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Topical negative pressure in managing severe peritonitis: A positive contribution? 被引量:1
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作者 Amin Ibrahim Amin Irshad A Shaikh 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第27期3394-3397,共4页
AIM: To assess the use of topical negative pressure (TNP) in the management of severe peritonitis. METHODS: This is a four-year prospective analysis from January 2005 to December 2008 of 20 patients requiring TNP ... AIM: To assess the use of topical negative pressure (TNP) in the management of severe peritonitis. METHODS: This is a four-year prospective analysis from January 2005 to December 2008 of 20 patients requiring TNP following laparotomy for severe peritonitis. RESULTS: There were 11 males with an average age of (59.3 ± 3.95) years. Nine had a perforated viscus, five had anastomotic leaks, three had iatrogenic bowel injury, and a further three had severe pelvic inflammatory disease. TNP and the VAC Abdominal Dressing System were initially used. These were changed every two to three days. Abdominal closure was achieved in 15/20 patients within 4.53 ± 1.64 d. One patient required relaparotomy due to residual sepsis. Two patients with severe faecal peritonitis due to perforated diverticular disease received primary anastomosis at second look laparotomy, as sepsis and their general condition improved. In the remaining 5/20 cases, the abdomen was lee open due to bowel oedema and or abdominal wall oedema. Dressing was switched to TNP and VAC GranuFoam . Three of the five patients returned a few months later for abdominal wall reconstruction and restoration of intestinal continuity. Two patients developed intestinal fistulae. All 20 patients survived.CONCLUSION: The use of TNP is safe. Further studies are needed to assess its value in managing these difficult cases. 展开更多
关键词 Severe peritonitis Open abdomen Topicalnegative pressure VAC Abdominal Dressing System VAC GranuFoam
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Insulin and heparin in treatment of hypertriglyceridemia-induced pancreatitis 被引量:24
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作者 Pankaj Jain Ramesh Roop Rai +2 位作者 Harsh Udawat Sandeep Nijhawan Amit Mathur 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第18期2642-2643,共2页
TO THE EDITOR We read with great interest the case report, “Hypertriglyceridernia -induced pancreatitis: A case-based review” by Gan eta]11 in the November 2006 issue of World Journal of Gastroenterology. We agree ... TO THE EDITOR We read with great interest the case report, “Hypertriglyceridernia -induced pancreatitis: A case-based review” by Gan eta]11 in the November 2006 issue of World Journal of Gastroenterology. We agree that in acute setting, pancreatitis due to hyper- triglyceridemia (HTG) should be ruled out as it is a treat-able and preventable condition. It needs to be treated conservatively along with measures to lower the triglyceride level. The various modalities to treat hypertriglyceridemia are plasmapheresis, insulin and heparin, purified apo C Ⅱ, and fibric acid derivatives^[2-5]. Plasmapheresis and purified apo C Ⅱ infusion are not easily available. There is limited literature about the efficacy of intravenous insulin and heparin, both of which can enhance lipoprotein lipase activity. 展开更多
关键词 GASTROENTEROLOGY
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Juvenile ferric iron prevents microbiota dysbiosis and colitis in adult rodents 被引量:4
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作者 Chourouk Ettreiki Pascale Gadonna-Widehem +3 位作者 Irène Mangin Mose Coёffier Carine Delayre-Orthez Pauline M Anton 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第21期2619-2629,共11页
AIM: To assess whether juvenile chronic ferric iron ingestion limit colitis and dysbiosis at adulthood in rats and mice. METHODS: Two sets of experiments were designed. In the first set, recently weaned mice were eith... AIM: To assess whether juvenile chronic ferric iron ingestion limit colitis and dysbiosis at adulthood in rats and mice. METHODS: Two sets of experiments were designed. In the first set, recently weaned mice were either orally administered ferrous (Fe2+) iron salt or ferric (Fe3+) microencapsulated iron for 6 wk. The last week of experiments trinitrobenzene sulfonic acid (TNBS) colitis was induced. In the second set, juvenile rats received the microencapsulated ferric iron for 6 wk and were also submitted to TNBS colitis during the last week of experiments. In both sets of experiments, animals were sacrificed 7 d after TNBS instillation. Severity of the inflammation was assessed by scoring macroscopic lesions and quantifying colonic myeloperoxidase (MPO) activity. Alteration of the microflora profile was estimated usingquantitative polymerase chain reaction (qPCR) by measuring the evolution of total caecal microflora, Bacteroidetes, Firmicutes and enterobacteria. RESULTS: Neither ferrous nor ferric iron daily exposures at the juvenile period result in any effect in control animals at adulthood although ferrous iron repeated administration in infancy limited weight gain. Ferrous iron was unable to limit the experimental colitis (1.71 ± 0.27 MPO U/mg proteinvs 2.47 ± 0.22 MPO U/mg protein in colitic mice). In contrast, ferric iron significantly prevented the increase of MPO activity (1.64 ± 0.14 MPO U/mg protein) in TNBS-induced colitis. Moreover, this positive effect was observed at both the doses of ferric iron used (75 and 150 mg/kg per day po - 6 wk). In the study we also compared, in both rats and mice, the consequences of chronic repeated low level exposure to ferric iron (75 mg/kg per day po - 6 wk) on TNBS-induced colitis and its related dysbiosis. We confirmed that ferric iron limited the TNBS-induced increase of MPO activity in both the rodent species. Furthermore, we assessed the ferric iron incidence on TNBS-induced intestinal microbiota dysbiosis. At first, we needed to optimize the isolation and quantify DNA copy numbers using standard curves to perform by qPCR this interspecies comparison. Using this approach, we determined that total microflora was similar in control rats and mice and was mainly composed of Firmicutes and Bacteroidetes at a ratio of 10/1. Ferric juvenile administration did not modify the microflora profile in control animals. Total microflora numbers remained unchanged whichever experimental conditions studied. Following TNBS-induced colitis, the Firmicutes/Bacteroidetes ratio was altered resulting in a decrease of the Firmicutes numbers and an increase of the Bacteroidetes numbers typical of a gut inflammatory reaction. In parallel, the subdominant population, the enterobacteria was also increased. However, ferric iron supplementation for the juvenile period prevented the increase of Bacteroidetes and of enterobacteria numbers consecutive to the colitis in both the studied species at adulthood.CONCLUSION: Rats and mice juvenile chronic ferric iron ingestion prevents colitis and dysbiosis at adulthood as assessed by the first interspecies comparison. 展开更多
关键词 Chronic ferric iron supplementation Experimental colitis Microflora dysbiosis Rat Mice
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Ameliorative effects of bombesin and neurotensin on trinitrobenzene sulphonic acid-induced colitis,oxidative damage and apoptosis in rats 被引量:14
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作者 Alper Akcan Sebahattin Muhtaroglu +5 位作者 Hulya Akgun Hizir Akyildiz Can Kucuk Erdogan Sozuer Alper Yurci Namik Yilmaz 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第8期1222-1230,共9页
AIM:To investigate the effects of bombesin (BBS) and neurotensin (NTS) on apoptosis and colitis in an ulcerative colitis model. METHODS:In this study, a total of 50 rats were divided equally into 5 groups. In the cont... AIM:To investigate the effects of bombesin (BBS) and neurotensin (NTS) on apoptosis and colitis in an ulcerative colitis model. METHODS:In this study, a total of 50 rats were divided equally into 5 groups. In the control group, no colitis induction or drug administration was performed. Colitis was induced in all other groups. Following the induction of colitis, BBS, NTS or both were applied to three groups of rats. The remaining group (colitis group) received no treatment. On the 11th d after induction of colitis and drug treatment, blood samples were collected for TNF-α and IL-6 level studies. Malondialdehyde (MDA), carbonyl, myeloperoxidase (MPO) and caspase-3 activities, as well as histopathological findings, evaluated in colonic tissues. RESULTS:According to the macroscopic and microscopic findings, the study groups treated with BBS, NTS and BBS + NTS showed significantly lower damage and inflammation compared with the colitis group (macroscopic score, 2.1 ± 0.87, 3.7 ± 0.94 and 2.1 ± 0.87 vs 7.3 ± 0.94;microscopic score, 2.0 ± 0.66, 3.3 ± 0.82 and 1.8 ± 0.63 vs 5.2 ± 0.78, P < 0.01). TNF-α and IL-6 levels were increased significantly in all groups compared with the control group. These increases were significantly smaller in the BBS, NTS and BBS + NTS groups compared with the colitis group (TNF-α levels, 169.69 ± 53.56, 245.86 ± 64.85 and 175.54 ± 42.19 vs 556.44 ± 49.82;IL-6 levels, 443.30 ± 53.99, 612.80 ± 70.39 and 396.80 ± 78.43 vs 1505.90 ± 222.23, P < 0.05). The colonic MPO and MDA levels were significantly lower in control, BBS, NTS and BBS + NTS groups than in the colitis group (MPO levels, 24.36 ± 8.10, 40.51 ± 8.67 and 25.83 ± 6.43 vs 161.47 ± 38.24;MDA levels, 4.70 ± 1.41, 6.55 ± 1.12 and 4.51 ± 0.54 vs 15.60 ± 1.88, P < 0.05). Carbonyl content and caspase-3 levels were higher in the colitis and NTS groups than in control, BBS and BBS + NTS groups (carbonyl levels, 553.99 ± 59.58 and 336.26 ± 35.72 vs 209.76 ± 30.92, 219.76 ± 25.77 and 220.34 ± 36.95;caspase-3 levels, 451.70 ± 68.27 and 216.20 ± 28.17 vs 28.60 ± 6.46, 170.50 ± 32.37 and 166.50 ± 30.95, P < 0.05). CONCLUSION:The results of this study suggest BBS and NTS, through their anti-inflammatory actions, support the maintenance of colonic integrity and merit consideration as potential agents for ameliorating colonic inflammation. 展开更多
关键词 BOMBESIN NEUROTENSIN COLITIS APOPTOSIS
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Atherosclerosis lesion is accelerated by persistent systemic inflammation but attenuated by saponins from Panax Notoginseng in rabbits
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作者 Jia Yi Li Xiaohui Liu Ya Zhang Haigang 《Journal of Medical Colleges of PLA(China)》 CAS 2008年第1期38-44,共7页
Objective: To explore the roles of persistent systemic inflammation in atherosclerosis and the effects of saponins of Panax Notoginseng (PNS) on this process in rabbits. Methods: Thirty rabbits were divided random... Objective: To explore the roles of persistent systemic inflammation in atherosclerosis and the effects of saponins of Panax Notoginseng (PNS) on this process in rabbits. Methods: Thirty rabbits were divided randomly and equally into 6 groups, i.e., control, high-fat diet, inflammation, aspirin, PNS and simple-inflammation group. All the animals except that in control group and simple-inflammation group were fed with high-fat diet for 8 weeks. Based on that, rabbits in inflammation, aspirin and PNS groups were treated with zymosan injection (10 mg/kg, i.p.). Normal saline was given to rabbits in control group. Besides zymosan injection, animals in aspirin and PNS group were administrated with aspirin (12 mg/kg, i.g.) and PNS (120 mg/kg, i.g.) respectively. The animals in simple-inflammation group were treated with zymosan injection (10mg/kg, i.p.) and fed with normal diet. The atherosclerosis lesion in aortas was observed by Sudan IV staining. Serum total cholesterol, triglyceride (TG), TNF-α and activity of post-heparin lipoprotein lipase (LPL) were measured at the end of the 4th and 8th week after an overnight fast. Results: Compared with high-fat diet group, the area of atherosclerosis lesion, serum TG and TNF-α were markedly increased in rabbits of inflammation group, and the activity of LPL was decreased remarkably. Serum TNF-α level was negatively correlated with the activity of post-heparin LPL (r=0.708, P〈0.01). The area of atherosclerosis, serum TG and TNF-α were decreased in aspirin and PNS group compared with that in inflammation group, and the activity of LPL was increased remarkably. Compared with control group, serum TG and TNF-α were markedly increased in simple-inflammation group, while LPL activity was decreased. Atherosclerotic lesion did not occur in simple-inflammation group. Conclusion: Persistent systemic inflammation could accelerate the formation of atherosclerosis lesion in aortas, which partly depend on the decreasing of the activity of post-heparin lipoprotein lipase. PNS could improve the changes caused by inflammation. 展开更多
关键词 Atherosclerosis Saponins of panax notoginseng Lipoprotein lipase Inflammation
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Prophylactic effect of glyceryl trinitrate on post-endoscopic retrograde cholangiopancreatography pancreatitis:A randomized placebo-controlled trial 被引量:8
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作者 Jian-Yu Hao Dong-Fang Wu Yue-Zeng Wang Ying-Xin Gao Hai-Po Lang Wei-Zhen Zhou 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第3期366-368,共3页
AIM:To examine the prophylactic effect of glyceryl trinitrate on post-endoscopic retrograde cholangiopancreatography(ERCP) pancreatitis and hyperamylasemia.METHODS:Patients scheduled for ERCP were randomly divided int... AIM:To examine the prophylactic effect of glyceryl trinitrate on post-endoscopic retrograde cholangiopancreatography(ERCP) pancreatitis and hyperamylasemia.METHODS:Patients scheduled for ERCP were randomly divided into study group and placebo group.Patients in study group and placebo group were treated with 5 mg glyceryl trinitrate and 100 mg vitamin C,respectively,5 min before endoscopic maneuvers.RESULTS:A total of 74 patients were enrolled in the final analysis.Post-ERCP pancreatitis occurred in 3 patients(7.9%) of the study group and 9 patients(25%) in the placebo group(P = 0.012).Hyperamylasemia occurred in 8 patients of the study group(21.1%) and 13 patients(36.1%) of the placebo group(P = 0.037).CONCLUSION:Glyceryl trinitrate before ERCP can effectively prevent post-ERCP and hyperamylasemia. 展开更多
关键词 Glyceryl trinitrate CHOLANGIOPANCREATOGRAPHY Endoscopic retrograde PANCREATITIS
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Effects of Changtai granules, a traditional compound Chinese medicine, on chronic trinitrobenzene sulfonic acid-induced colitis in rats 被引量:5
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作者 Yong-Bing Cao,Jun-Dong Zhang,Lan Yan,De-Jun Wang,Xin-Ming Jia,Ping-Hui Gao,Ming-He Cheng,Zheng Xu,Yan Wang,Yuan-Ying Jiang,Department of Pharmacology,School of Pharmacy,Second Military Medical University,Shanghai 200433,China Ya-Ying Diao,Department of Pharmacy,Changhai Hospital,Second Military Medical University,Shanghai 200433,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第23期3539-3543,共5页
AIM: To study the effects of Changtai granules (CTG), a traditional compound Chinese medicine, on chronic trinitrobenzene sulfonic acid-induced colitis in rats. METHODS: Healthy adult Sprague-Dawley (SD) rats of both ... AIM: To study the effects of Changtai granules (CTG), a traditional compound Chinese medicine, on chronic trinitrobenzene sulfonic acid-induced colitis in rats. METHODS: Healthy adult Sprague-Dawley (SD) rats of both sexes, weighing 250-300 g, were employed in the present study. The rat colitis models were induced by 2, 4,6-trinitrobenzene sulfonic acid (TNBS) enemas at a concentration of 100 mg/kg in 50% ethanol. The experimental animals were randomly divided into dexamethasone (DX) treatment, CTG treatment, and model control groups, which were intracolicly treated daily with DX (0.2 mg/kg), CTG at doses of 2.9, 5.7 and 11.4 g crude drug/kg, and the equal amount of saline respectively from 6 h following induction of the colitis in rats inflicted with TNBS to the end of study. A normal control group of rats treated without TNBS but saline enema was also included in the study. After 3 wk of treatment, the animals were assessed for colonal inflammatory and ulcerative responses with respect to mortality, frequency of diarrhea, histology and myeloperoxidase activity (MPO).RESULTS: The therapeutic effect of CTG on ulcerative colitis (UC) was better than DX. CTG effectively inhibited the activity of granulocytes, macrophages and monocytes in a dosedependent manner. Also it reduced MPO and formation of inflammation in colonic mucosal tissue. Furthermore, administration of CTG significantly prevented body mass loss and death, and decreased frequency of diarrhea in UC rats, when compared with the model control group rats.CONCLUSION: CTG would prove to be an ideal drug for chronic UC, and is warranted to be studied further. 展开更多
关键词 Ulcerative colitis Changtai granules 2 4 6-Trinitrobenzene sulfonic acid MYELOPEROXIDASE
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Correlation between expression of cyclooxygenase-2 and the presence of inflammatory cells in human primary hepatocellular carcinoma: Possible role in tumor promotion and angiogenesis 被引量:21
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作者 Melchiorre Cervello Daniela Foderà +4 位作者 Ada Maria Florena Maurizio Soresi Claudio Tripodo Natale D'Alessandro Giuseppe Montalto 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第30期4638-4643,共6页
AIM: To investigate the association of cyclooxygenase-2 (COX-2) expression with angiogenesis and the number and type of inflammatory cells (macrophages/Kupffer cells; mast cells) within primary hepatocellular car... AIM: To investigate the association of cyclooxygenase-2 (COX-2) expression with angiogenesis and the number and type of inflammatory cells (macrophages/Kupffer cells; mast cells) within primary hepatocellular carcinoma (HCC) tissues and adjacent non-tumorous (NT) tissues. METHODS: Immunohistochemistry for COX-2, CD34, CD68 and mast cell tryptase (MCT) was performed on 14 well-characterized series of liver-cirrhosis-associated HCC patients. COX-2 expression and the number of inflammatory cells in tumor lesions and surrounding liver tissues of each specimen were compared. Moreover, COX-2, CD34 staining and the number of inflammatory cells in areas with different histological degrees within each tumor sample were comparatively analyzed. RESULTS: The percentage of COX-2 positive cells was significantly higher in NT tissues than in tumors. COX-2 expression was higher in well-differentiated HCC than in poorly-differentiated tissues. Few mast cells were observed within the tumor mass, whereas a higher number was observed in the surrounding tissue, especially in peri-portal spaces of NT tissues. Abundant macrophages/ Kupffer cells were observed in NT tissues, whereas the number of cells was significantly lower in the tumor mass. However, a higher cell number was observed in the welldifferentiated tumor and progressively decreased in relation to the differentiation grade. Within the tumor, a positive correlation was found between COX-2 expression and the number of macrophages/Kupffer cells and mastcells. Moreover, there was a positive correlation between CD34 and COX-2 expression in tumor tissues. Comparison between well- and poorly-differentiated HCC showed that the number of CD34-positive cells decreased with dedifferentiation. However, COX-2 was the only independent variable showing a positive correlation with CD34 in a multivariate analysis. CONCLUSION: The presence of inflammatory cells and COX-2 expression in liver tumor suggests a possible relationship with tumor angiogenesis. COX-2 expressing cells and the number of macrophages/Kupffer cells and mast cells decrease with progression of the disease. 展开更多
关键词 COX-2 HCC ANGIOGENESIS Mast cells Macro-phages
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Effect of interferon and ribavirin combined with amantadine in interferon and ribavirin non-responder patients with chronic hepatitis C (genotype 1) 被引量:1
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作者 Dilek Oguz Bahattin Cicek +5 位作者 Levent Filik Bulent Odemis Mesut Kilic Engin Altintas Neslihan Zengin Emin Altiparmak 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第4期580-583,共4页
AIM: To evaluate the efficacy of amantadine plus interferonalpha and ribavirin in non-responder patients with chronic hepatitis C.METHODS: Twenty-six non-responder patients received the regimen of IFN-α-2a at a dose ... AIM: To evaluate the efficacy of amantadine plus interferonalpha and ribavirin in non-responder patients with chronic hepatitis C.METHODS: Twenty-six non-responder patients received the regimen of IFN-α-2a at a dose of 6 million units three times a week, 1 000-1 200 mg of ribavirin daily, and 200 mg of amantadine daily in divided doses over 48 wk. After the end of treatment, at the 72nd wk, a sustained viral response rate was determined.RESULTS: An early (after 12 wk of therapy) response was seen in 34.6% (9/26) of patients. Response rate at the 24th wk was 42.3% (11/26). End of treatment response (ETR) was 53.8% (14/26). Sustained viral response (SVR) was 42.3% (11/26). There was a statistically significant difference between 0 and 12 wk (P = 0.04), 0 and 24 wk (P = 0.01), 0 and 48 wk (P = 0.00), and 0 and 72 wk (P = 0.001). No patient had severe adverse effects during the treatment.CONCLUSION: Combination regimen of interferon-α,ribavirin and amantadine can enhance sustained viral response on IFN-α and ribavirin non-responder patients with HCV. Triple therapy with amantadine should be evaluated in further studies. 展开更多
关键词 Chronic hepatitis C IFN-α-2a RIBAVIRIN
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Preventive and therapeutic effects of NF-kappaB inhibitor curcumin in rats colitis induced by trinitrobenzene sulfonic: acid 被引量:19
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作者 Yan-TingJian Guo-FengMai +3 位作者 Ji-DeWang Ya-LiZhang Rong-ChengLuo Yong-XinFang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第12期1747-1752,共6页
AIM: To ascertain the molecule mechanism of nuclear factor-KB (NF-κB) inhibitor curcumin preventive and therapeutic effects in rats' colitis induced by trinitrobenzene sulfonic acid (TNBS). METHODS: Sixty rats wi... AIM: To ascertain the molecule mechanism of nuclear factor-KB (NF-κB) inhibitor curcumin preventive and therapeutic effects in rats' colitis induced by trinitrobenzene sulfonic acid (TNBS). METHODS: Sixty rats with TNBS-induced colitis were treated with 2.0% curcumin in the diet. Thirty positive control rats were treated with 0.5% sulfasalazine (SASP). Thirty negative control rats and thirty model rats were treated with general diet. Changes of body weight together with histological scores were evaluated. Survival rates were also evaluated. Cell nuclear NF-κB activity in colonic mucosa was evaluated by using electrophoretic mobility shift assay. Cytoplasmic IκB protein in colonic mucosa was detected by using Western Blot analysis. Cytokine messenger expression in colonic tissue was assessed by using semiquantitative reverse-transcription polymerase chain reaction. RESULTS: Treatment with curcumin could prevent and treat both wasting and histopathologic signs of rats with TNBS-induced intestinal inflammation. In accordance with these findings, NF-κB activation in colonic mucosa was suppressed in the curcumin-treated groups. Degradations of cytoplasmic IκB protein in colonic mucosa were blocked by curcumin treatment. Proinflammatory cytokine messenger RNA expression in colonic mucosa was also suppressed. CONCLUSION: This study shows that NF-κB inhibitor curcumin could prevent and improve experimental colitis in murine model with inflammatory bowel disease (IBD). The findings suggest that NF-κB inhibitor curcumin could be a potential target for the patients with IBD. 展开更多
关键词 IBD CURCUMIN TNBS NF-κB
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